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peanotter
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Re: CLONE WARS [Re: A.k.a]
#26562564 - 03/28/20 05:53 AM (4 years, 3 months ago) |
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So if we're not supposed to clone a clone (per RR), how would we narrow down the specific phenotype we're after?
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,540
Loc: Terrapin station
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Re: CLONE WARS [Re: A.k.a]
#26562672 - 03/28/20 08:26 AM (4 years, 3 months ago) |
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Quote:
A.k.a said: lol that kk tub looks like an alien nest with all the myc tentacles and fat egg pins.
Looks like they’ll be nice and beefy.
I wish I could get my old AA+ genetics back. They in my sig. They were the same way. Got really fat bottom. Pins looked like them ape pins.
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A.k.a
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Quote:
peanotter said: So if we're not supposed to clone a clone (per RR), how would we narrow down the specific phenotype we're after?
I’ve been trying to get a solid answer.
So far I think you clone the one you like, grow it out and take a print from one that looks similar, grow that and it should have a good chance of more of that pheno and then you clone and repeat.
However multiple people have said they clone a clone of a clone of a clone no problem.
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LAGM2020     
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Buddaking
Genetics



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Re: CLONE WARS [Re: A.k.a] 1
#26562824 - 03/28/20 09:46 AM (4 years, 3 months ago) |
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When I get a good clone I just work out each sector till I Isolate the genitic trait I desire. Ya end up with a lot of petri's so labeling & space to slow em down in cold storage is important. Once I find the "one" I go back & clear out the fridge of unwanted & make atleast 3 slants to fall back on.
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 AMU Q & A
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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I lost / abandoned my original project but these both came from an albino cambo streak plate. One really fat pin and one really long pin. Going to run them side by side and see what’s good.

I’ve gotta get my cold storage going.. Juggling cultures around is a bitch.
-------------------- Tek's I use LAGM2020
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,540
Loc: Terrapin station
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Quote:
Hobbit GDF said: Ok so I'm working on a few and font have pics of some. But I do of my "king kong" APE clone. First time me fruiting this clone. I also have another ape clone and a swab of one fruiting. Idk if the swab shoebox will make apes. They look like it so far.
Here's "KingKong" ...


And here is the first shoebox of the KK

Nice fat pins. I just need to get them clustered. Maybe cross with a more clustering culture of ape?
I'm working on Melmak 
Pe6. This is clone tub 
Aa+ . 
Golden halo. 
Tailfeathers & white TF (no parent pic) LPEU (no pic)(I'm excited about this one), the fruit were huge and the mono flushed amazingly. Costa rican (clone sucked inch tall). 
mystery variety -clone tub, that turned out well. 
Well that's what I have to show anyhow.
Ok harvested the ape clone sb. Wet weight was only 188g. Sharpie for size scale.
 None was as big as parent. I cloned it again. How many times can I do that? I will just find out. I'll restart from next set spores.
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A.k.a
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Keeping this thing going.
So for lagm I did my first toc grows.
Each shoebox had a great cluster that I cloned.

Both were spawned 7 and 4 days ago.
The older one was knotting on day 6 and the other one I ran out of coir and didn’t add a top layer til the end of the second day. 16 hours after spawning it was already popping rhizo off the exposed grains. Added the coir and today, two days later, it’s to the surface on about 70%.
High hopes for both these guys.
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LAGM2020     
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trubblesome
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Re: CLONE WARS [Re: A.k.a]
#26599271 - 04/13/20 11:29 PM (4 years, 2 months ago) |
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Nice work Hobbit and Aka
I'll finally be spawning my "Nip B+" tomorrow. Shoebox. Will print, grow and hopefully get a larger nip fruit from that batch to clone and so on until I have a reliably nip cap'd culture.
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woofwoof
such mushrooms!



Registered: 01/04/19
Posts: 1,127
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What traits is everybody after for cloning? Large fruit? Potency? Canopies? Canopies are surface condition dependent or can clones recreate canopies?
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A.k.a
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Potency speed and yield for me.
So these are the clones spawned on the 6th and 9th, the younger one top layered on the 11th.

More advanced one is the cluster still on the sub in the earlier pic.
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trubblesome
Stranger



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Re: CLONE WARS [Re: A.k.a]
#26602976 - 04/15/20 01:18 PM (4 years, 2 months ago) |
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Okay, spawned Nip B+ and Big GT today. These are the deepest shoeboxes I've ever done. spawned 1:2.5 with a half quart casing layer. forgot to get pics of the jars pre-spawn.
Nip B+ original:

The Clone boxes:

I hit the jackpot on clusters with this PESA tub. Unfortunately I also hit the jackpot on mold. I caught it before it went green but I cut this whole section out - the casing wasn't colonizing and the tub began to get a mildew smell. I cut it out and put it in a bowl and on close examination, what appeared to have been knots was the beginning of some kind of mold growing. I put the bowl outside and the next day it was green, maybe trich
Anyway I think I bought myself enough time to get a decent harvest as well as get a couple clones from these clusters. I think the clusters came from how I chose the mycelium from the plates but I'll leave that for another post. I will probably do something similar with the clones I gather from this tub...
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Camera93
We got dicks like Jesus



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Re: CLONE WARS [Re: A.k.a]
#26602989 - 04/15/20 01:21 PM (4 years, 2 months ago) |
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Quote:
A.k.a said: Potency speed and yield for me.
So these are the clones spawned on the 6th and 9th, the younger one top layered on the 11th.

More advanced one is the cluster still on the sub in the earlier pic.
How are you testing for potency? I keep getting ready to ask about potency on TOC but I know I'll get "the cube is a cube/have to clone and isolate for consistent potency/ it's all multi-spore and random hit&miss w/o isolating"
but if someone cares to toss their anecdotal evidence into the mix for me  (in comparison to rustywhytes)**
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
Edited by Camera93 (04/15/20 01:22 PM)
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woofwoof
such mushrooms!



Registered: 01/04/19
Posts: 1,127
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I am hoping to do a clone of my RW that are about to pin. I suppose cloning for potency is gunna be hard because there is no way to know until you clone them then harvest and dose.
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A.k.a
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lol Idk either really.
I guess yeah wait til you find one that does well and eat some hoping it’s acceptable.
I’ve heard of people taking a tissue sample then eating the mushroom also but I’m not sure how reliable it is.
My main question is with ms grows you could pick two random shrooms and one could be five times stronger than the other. So is a clone grow the same just with a smaller range in potency?
It doesn’t seem like they would all be the same strength unless the culture was super narrowed down.
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trubblesome
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Re: CLONE WARS [Re: A.k.a] 1
#26606172 - 04/16/20 04:38 PM (4 years, 2 months ago) |
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Okay here we go. Woke up to this

Picked 8 of the largest clustered shrooms, and then one fat side pin for cloning. side pin turned out to be in a cluster too. So here begin my PESA culture projects.

These are the mushrooms I picked. Labeled in rough order of size 1-7, corresponding with the labels on the dishes. I didn't think to arrange them and label by size until after I was all up in the SAB. When I chose mycelium to inoculate the master jar with for this grow, I took from a plate that held three different transfers from the 3 fastest rhizo growths on the previous plate. when I inoculated the master jar, I took a wedge from each intersection of these growths, in the place where I could see that the mycelium was compatible and clearly linking. I'm hoping that this selection method and the genetics isolated together had something to do with the clustering. So I'm going to do something similar with the clones. I took clones by taking a small prism shaped piece out of each mushroom's cap tissue:

I make my slices in the order and direction indicated in the photo - Slice 1 and 2 are done first because it's easier to cut across the grain when you do them first. Slice 3 and 4 are angled toward each other so that the resulting piece of tissue is kind of a prism shape and is removed easily if not when I make my last slice, then by simply poking it with the tip of the scalpel blade. To get to this point, I simply grab the mushroom at the base of the stem and rip longways. It usually is happy to break right down the middle. Halfway through I adjust the way I hold it so that the cap is upward, this way nothing from my hands or on the outside of the stem can fall in to the open middle portion of the cap once I split it open. keep the cap elevated until you make your cut, at which point only the scalpel should move above it. I figure this way increases the odds of not carrying stray cube spores or anything wack like mold and bacteria with it.

I placed the cuttings on their own halves of split petri dishes. I think I may isolate "Fat Boy" on its own and do a solo shoebox with it to prove its genetics. The rest are going to be transferred to combo dishes to find matching pairs once I get something clean from each.
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A.k.a
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Coming along nicely.
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trubblesome
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Re: CLONE WARS [Re: A.k.a]
#26624495 - 04/24/20 11:18 AM (4 years, 2 months ago) |
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clone dishes looking good, clean as fuck, which is a relief since I had a bad SAB session last week and had to make a ton of transfers last night to get away from shit on dishes. I also realized I could just drop all these in to their own jars or in to one bag and mix the genetics at spawn or inoculation rather than waste time dicking around with enough plates to have every combo covered. so I'll probably do that. I'm gonna drop fat boy in its own grain jar at least.

The clone shoeboxes are about to pop. guessing I'll have a pinset going monday. hopefully the nip was genetic. I don't even remember what "Big GT" looked like, so hopefully it's just fucking big and has a nice cap for printing.
Nip B+:

Big GT:
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A.k.a
Stranger



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Oh yeah those are ready for pins.
Forgot to update here but my toc clones were eh. One decent at like 300 I think and the other like 180.

They came from such good clusters too.
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Wolfenstein
Learning & Yearning


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Re: CLONE WARS [Re: A.k.a]
#26624723 - 04/24/20 01:03 PM (4 years, 2 months ago) |
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Ah shit buddy you're getting me all sorts of excited
-------------------- Anything and everything I say, write, and/or imply is purely false and is only done so with the intent to enhance my acting career. Molon Labe As above, so below
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trubblesome
Stranger



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I'd been having some trouble with FAE and humidity in the room my shroomies are in, started to get some side pins on the Nip B+, so I said fuck it and plopped both shoeboxes in the fruiting tent for my edibles since I don't have any fruiting right now and i've never done it with the actives.

Only problem is once I kicked the humidifier back on the temp dropped 10 degrees to around 60 and I didn't realize it til late at night. made some tweaks and no change the next morning. more tweaks and no change somehow. finally just rerouted the air flow with the plant tent and got it right today. but they had a couple days there in the low 60s that kinda stalled 'em out a bit. I worried that I fucked up the clone somehow too as they weren't very nipply but the nip came out a bit once the veil broke and the cap started to broaden:


Still not as nipply as the original. they are my favorite size though, 4 inches or so, fatter around than a sharpie, dense fruits. they'll probably look good dried. they're not the nipple clones of my dreams though, so back to the drawing board there. Plucked the ones with broken veils and left the rest to develop in better conditions.
so far "big GT" is just a kind of solid, meaty shroom that's about 4" high, but how much of that is from the temp drop who knows. We'll see how the rest of the developing shrooms do now that things are back to 70.
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