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InvisibleYogiBear
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Registered: 08/24/19
Posts: 845
YogiBear's Agar Help Line 🆘❗️❗️❗️
    #26447616 - 01/23/20 04:26 AM (4 years, 6 days ago)

HELP!! LOL

I'm going to be focusing all my efforts on my agar work this year and will need my own thread because I get so lost trying to keep up with the other agar threads due to the shear volume of questions and answers.

ALL I ASK IS THAT YOU PLEASE
USE THE QUOTE FEATURE WITH EVERY REPLY
SO I KNOW WHAT PLATE YOU ARE REFERRING TO

Please forgive the poor photo quality at the
moment better agar is on the way

😎👍👍

You guys are awesome and I thank you
in advance for any and all help.


Edited by YogiBear (01/26/20 07:45 PM)


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InvisibleYogiBear
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PE6 Thumb Print [Re: YogiBear]
    #26447622 - 01/23/20 04:36 AM (4 years, 6 days ago)

Best foot forward...

I was harvesting some PE6 a while back and had spore covered gloves so I said why not do a thumb print on agar to see what would happen. I had lots of black specks and white specks all over and I grabbed a white one asap and this is my results of my T1

New lighting and agar will help very soon with Picts.

Can you see anything bad? Where should I grab from?

I'm thinking 1 and 3 are good candidates



Edited by YogiBear (01/23/20 06:20 AM)


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OfflineMycoBrainz
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Re: PE6 Thumb Print [Re: YogiBear]
    #26447730 - 01/23/20 07:11 AM (4 years, 6 days ago)

Let it fruit in dish,  then take the little mushroom as a transfer. This will save you much time trying to isolate culture. Here's a example of what it looks like second transfer on my dish.
https://files.shroomery.org/files/20-004/978863923-20200119_090303.jpg


--------------------
:stoned:


PFC x Creeper


Lets Get Stoned


Edited by MycoBrainz (01/23/20 07:12 AM)


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OfflinecronicrFacebook
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
    #26448238 - 01/23/20 01:48 PM (4 years, 6 days ago)

This thread was moved from the user's journal.

Reason:
:super:


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OfflineMLPismyOPSEC
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Re: PE6 Thumb Print [Re: YogiBear]
    #26448630 - 01/23/20 05:18 PM (4 years, 6 days ago)

Quote:

YogiBear said:
I'm thinking 1 and 3 are good candidates






The picture is a little blurry but 1 and 3 look great to me, another day or two will give you a better idea!


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InvisibleYogiBear
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Re: PE6 Thumb Print [Re: MLPismyOPSEC]
    #26448978 - 01/23/20 08:45 PM (4 years, 6 days ago)

Quote:

MLPismyOPSEC said:
Quote:

YogiBear said:
I'm thinking 1 and 3 are good candidates






The picture is a little blurry but 1 and 3 look great to me, another day or two will give you a better idea!




Thanks for the info guys. Much appreciated.

Will post more Picts of other plates soon. They are much worse but Definitely worth a shot for my beginner efforts for practice anyway.

More to come ...


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InvisibleYogiBear
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: cronicr]
    #26448999 - 01/23/20 09:03 PM (4 years, 6 days ago)

Thanks cron 😎👍👍


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InvisibleComebackKid
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
    #26449013 - 01/23/20 09:15 PM (4 years, 6 days ago)

:threadmonitor:
                          :thumbup:


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InvisibleYogiBear
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: ComebackKid]
    #26449594 - 01/24/20 08:57 AM (4 years, 5 days ago)

First official PE6 clone as guided by CBK

Split stipe up to cap and dug out 2 pieces.



EDIT
See how I did that everyone... If this doesn't work I can blame CBK 🤣😆😛


Edited by YogiBear (01/24/20 07:56 PM)


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InvisibleYogiBear
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
    #26451359 - 01/25/20 07:19 AM (4 years, 4 days ago)

Rusty Whyte Plate 16 days old from swab

Part of my LAGM2020 grow that has been neglected

Looks clean to me but what do I know 🤪

Just grab anything??? I have no idea where to put numbers for reference



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InvisiblemushboyMDiscord
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
    #26451365 - 01/25/20 07:23 AM (4 years, 4 days ago)

Just cut anywhere. Save the plate tho. Soon it will pin and you can grab some easy clones


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InvisibleYogiBear
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: mushboy]
    #26452942 - 01/26/20 05:23 AM (4 years, 3 days ago)

Quote:

mushboy said:
Just cut anywhere. Save the plate tho. Soon it will pin and you can grab some easy clones




😎👍👍

Plate saved...



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OfflineeLeSDenes
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
    #26452951 - 01/26/20 05:36 AM (4 years, 3 days ago)

Yeah def keep the old plate for pin clones, I made many and they are usually very rhizomorph looking on the plates and very easy to isolate


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InvisibleYogiBear
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
    #26452956 - 01/26/20 05:41 AM (4 years, 3 days ago)

Next plate is PESA 🥰

Looking great. What would you guys suggest???

Looks like pins aren't far off.



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InvisibleYogiBear
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: eLeSDenes]
    #26452958 - 01/26/20 05:41 AM (4 years, 3 days ago)

Quote:

eLeSDenes said:
Yeah def keep the old plate for pin clones, I made many and they are usually very rhizomorph looking on the plates and very easy to isolate




Duly noted 😎👍👍


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InvisibleYogiBear
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
    #26453054 - 01/26/20 07:23 AM (4 years, 3 days ago)

Quote:

mushboy said:
Just cut anywhere. Save the plate tho. Soon it will pin and you can grab some easy clones




Going to do the same for this PESA IN 3 spots.

Quote:

YogiBear said:
Next plate is PESA 🥰

Looking great. What would you guys suggest???

Looks like pins aren't far off.






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Offlinetrubblesome
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
    #26453064 - 01/26/20 07:28 AM (4 years, 3 days ago)

if it were me doing three transfers i’d do these:



someone else might prefer others but that well differentiated band from 7:30ish-9 o’clock would be a winner from just about everywhere on it i think


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InvisibleYogiBear
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
    #26453189 - 01/26/20 09:11 AM (4 years, 3 days ago)

Quote:

YogiBear said:
Quote:

mushboy said:
Just cut anywhere. Save the plate tho. Soon it will pin and you can grab some easy clones




Going to do the same for this PESA IN 3 spots.

Quote:

YogiBear said:
Next plate is PESA 🥰

Looking great. What would you guys suggest???

Looks like pins aren't far off.









3x T1



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InvisibleYogiBear
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: trubblesome]
    #26453191 - 01/26/20 09:12 AM (4 years, 3 days ago)

Quote:

trubblesome said:
if it were me doing three transfers i’d do these:



someone else might prefer others but that well differentiated band from 7:30ish-9 o’clock would be a winner from just about everywhere on it i think




I did 2 of those and 1 different. This pict in different light and 3 days later showed something in one of these spots.

Got my new overhead light now and can see things better 👍


Edited by YogiBear (01/26/20 07:23 PM)


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InvisibleYogiBear
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
    #26464601 - 02/02/20 03:28 AM (3 years, 11 months ago)

Where do you guys think I should pull my T2s from on these?

Just grab and go?

Thanks in advance...

Rusty Whyte


PESA


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InvisibleLadysKnight
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
    #26464733 - 02/02/20 06:56 AM (3 years, 11 months ago)

Dodge the bacteria all around pesa. RW looks like mold.


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InvisibleYogiBear
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: LadysKnight]
    #26465650 - 02/02/20 05:10 PM (3 years, 11 months ago)

Quote:

LadysKnight said:
Dodge the bacteria all around pesa. RW looks like mold.




Thank you but I'm not sure how to apply this advise to my plates...



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InvisiblemushboyMDiscord
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
    #26465727 - 02/02/20 06:03 PM (3 years, 11 months ago)

id take them out of the bags for the pic. the rw looks like rw which ime looks iffy:lol:

the pesa has bacterial splooge action.

take better pics ya old geezer:oldman:


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InvisibleYogiBear
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Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: mushboy]
    #26465744 - 02/02/20 06:10 PM (3 years, 11 months ago)

Quote:

mushboy said:
id take them out of the bags for the pic. the rw looks like rw which ime looks iffy:lol:

the pesa has bacterial splooge action.

take better pics ya old geezer:oldman:




LOL

10-4 to the better picts

I'm doing 6 random transfers from each just to see what happens.


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