|
YogiBear



Registered: 08/24/19
Posts: 845
|
YogiBear's Agar Help Line 🆘❗️❗️❗️
#26447616 - 01/23/20 04:26 AM (4 years, 6 days ago) |
|
|
HELP!! LOL
I'm going to be focusing all my efforts on my agar work this year and will need my own thread because I get so lost trying to keep up with the other agar threads due to the shear volume of questions and answers.
ALL I ASK IS THAT YOU PLEASE USE THE QUOTE FEATURE WITH EVERY REPLY SO I KNOW WHAT PLATE YOU ARE REFERRING TO
Please forgive the poor photo quality at the moment better agar is on the way 😎👍👍
You guys are awesome and I thank you in advance for any and all help.
Edited by YogiBear (01/26/20 07:45 PM)
|
YogiBear



Registered: 08/24/19
Posts: 845
|
|
Best foot forward...
I was harvesting some PE6 a while back and had spore covered gloves so I said why not do a thumb print on agar to see what would happen. I had lots of black specks and white specks all over and I grabbed a white one asap and this is my results of my T1
New lighting and agar will help very soon with Picts.
Can you see anything bad? Where should I grab from?
I'm thinking 1 and 3 are good candidates
Edited by YogiBear (01/23/20 06:20 AM)
|
MycoBrainz
Mycotic



Registered: 03/14/15
Posts: 615
Loc: West Coast
Last seen: 4 months, 10 days
|
Re: PE6 Thumb Print [Re: YogiBear]
#26447730 - 01/23/20 07:11 AM (4 years, 6 days ago) |
|
|
Let it fruit in dish, then take the little mushroom as a transfer. This will save you much time trying to isolate culture. Here's a example of what it looks like second transfer on my dish. https://files.shroomery.org/files/20-004/978863923-20200119_090303.jpg
--------------------
PFC x Creeper Lets Get Stoned
Edited by MycoBrainz (01/23/20 07:12 AM)
|
cronicr


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
#26448238 - 01/23/20 01:48 PM (4 years, 6 days ago) |
|
|
This thread was moved from the user's journal.
Reason:
|
MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
Last seen: 10 days, 10 hours
|
Re: PE6 Thumb Print [Re: YogiBear]
#26448630 - 01/23/20 05:18 PM (4 years, 6 days ago) |
|
|
Quote:
YogiBear said: I'm thinking 1 and 3 are good candidates

The picture is a little blurry but 1 and 3 look great to me, another day or two will give you a better idea!
|
YogiBear



Registered: 08/24/19
Posts: 845
|
|
Quote:
MLPismyOPSEC said:
Quote:
YogiBear said: I'm thinking 1 and 3 are good candidates

The picture is a little blurry but 1 and 3 look great to me, another day or two will give you a better idea!
Thanks for the info guys. Much appreciated.
Will post more Picts of other plates soon. They are much worse but Definitely worth a shot for my beginner efforts for practice anyway.
More to come ...
|
YogiBear



Registered: 08/24/19
Posts: 845
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: cronicr]
#26448999 - 01/23/20 09:03 PM (4 years, 6 days ago) |
|
|
Thanks cron 😎👍👍
|
ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
#26449013 - 01/23/20 09:15 PM (4 years, 6 days ago) |
|
|
|
YogiBear



Registered: 08/24/19
Posts: 845
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: ComebackKid]
#26449594 - 01/24/20 08:57 AM (4 years, 5 days ago) |
|
|
First official PE6 clone as guided by CBK
Split stipe up to cap and dug out 2 pieces.

EDIT See how I did that everyone... If this doesn't work I can blame CBK 🤣😆😛
Edited by YogiBear (01/24/20 07:56 PM)
|
YogiBear



Registered: 08/24/19
Posts: 845
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
#26451359 - 01/25/20 07:19 AM (4 years, 4 days ago) |
|
|
Rusty Whyte Plate 16 days old from swab
Part of my LAGM2020 grow that has been neglected
Looks clean to me but what do I know 🤪
Just grab anything??? I have no idea where to put numbers for reference
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,274
Loc: where?
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
#26451365 - 01/25/20 07:23 AM (4 years, 4 days ago) |
|
|
Just cut anywhere. Save the plate tho. Soon it will pin and you can grab some easy clones
|
YogiBear



Registered: 08/24/19
Posts: 845
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: mushboy]
#26452942 - 01/26/20 05:23 AM (4 years, 3 days ago) |
|
|
Quote:
mushboy said: Just cut anywhere. Save the plate tho. Soon it will pin and you can grab some easy clones
😎👍👍
Plate saved...
|
eLeSDenes
Mycelium Expander




Registered: 05/25/11
Posts: 955
Last seen: 1 year, 11 months
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
#26452951 - 01/26/20 05:36 AM (4 years, 3 days ago) |
|
|
Yeah def keep the old plate for pin clones, I made many and they are usually very rhizomorph looking on the plates and very easy to isolate
|
YogiBear



Registered: 08/24/19
Posts: 845
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
#26452956 - 01/26/20 05:41 AM (4 years, 3 days ago) |
|
|
Next plate is PESA 🥰
Looking great. What would you guys suggest???
Looks like pins aren't far off.
|
YogiBear



Registered: 08/24/19
Posts: 845
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: eLeSDenes]
#26452958 - 01/26/20 05:41 AM (4 years, 3 days ago) |
|
|
Quote:
eLeSDenes said: Yeah def keep the old plate for pin clones, I made many and they are usually very rhizomorph looking on the plates and very easy to isolate
Duly noted 😎👍👍
|
YogiBear



Registered: 08/24/19
Posts: 845
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
#26453054 - 01/26/20 07:23 AM (4 years, 3 days ago) |
|
|
Quote:
mushboy said: Just cut anywhere. Save the plate tho. Soon it will pin and you can grab some easy clones
Going to do the same for this PESA IN 3 spots.
Quote:
YogiBear said: Next plate is PESA 🥰
Looking great. What would you guys suggest???
Looks like pins aren't far off.

|
trubblesome
Stranger



Registered: 11/09/19
Posts: 406
Last seen: 10 months, 17 days
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
#26453064 - 01/26/20 07:28 AM (4 years, 3 days ago) |
|
|
if it were me doing three transfers i’d do these:

someone else might prefer others but that well differentiated band from 7:30ish-9 o’clock would be a winner from just about everywhere on it i think
|
YogiBear



Registered: 08/24/19
Posts: 845
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
#26453189 - 01/26/20 09:11 AM (4 years, 3 days ago) |
|
|
Quote:
YogiBear said:
Quote:
mushboy said: Just cut anywhere. Save the plate tho. Soon it will pin and you can grab some easy clones
Going to do the same for this PESA IN 3 spots.
Quote:
YogiBear said: Next plate is PESA 🥰
Looking great. What would you guys suggest???
Looks like pins aren't far off.

3x T1
|
YogiBear



Registered: 08/24/19
Posts: 845
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: trubblesome]
#26453191 - 01/26/20 09:12 AM (4 years, 3 days ago) |
|
|
Quote:
trubblesome said: if it were me doing three transfers i’d do these:

someone else might prefer others but that well differentiated band from 7:30ish-9 o’clock would be a winner from just about everywhere on it i think
I did 2 of those and 1 different. This pict in different light and 3 days later showed something in one of these spots.
Got my new overhead light now and can see things better 👍
Edited by YogiBear (01/26/20 07:23 PM)
|
YogiBear



Registered: 08/24/19
Posts: 845
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
#26464601 - 02/02/20 03:28 AM (3 years, 11 months ago) |
|
|
Where do you guys think I should pull my T2s from on these?
Just grab and go?
Thanks in advance...
Rusty Whyte
PESA
|
LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,670
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
#26464733 - 02/02/20 06:56 AM (3 years, 11 months ago) |
|
|
Dodge the bacteria all around pesa. RW looks like mold.
|
YogiBear



Registered: 08/24/19
Posts: 845
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: LadysKnight]
#26465650 - 02/02/20 05:10 PM (3 years, 11 months ago) |
|
|
Quote:
LadysKnight said: Dodge the bacteria all around pesa. RW looks like mold.
Thank you but I'm not sure how to apply this advise to my plates...
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,274
Loc: where?
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: YogiBear]
#26465727 - 02/02/20 06:03 PM (3 years, 11 months ago) |
|
|
id take them out of the bags for the pic. the rw looks like rw which ime looks iffy
the pesa has bacterial splooge action.
take better pics ya old geezer
|
YogiBear



Registered: 08/24/19
Posts: 845
|
Re: YogiBear's Agar Help Line 🆘❗️❗️❗️ (moved) [Re: mushboy]
#26465744 - 02/02/20 06:10 PM (3 years, 11 months ago) |
|
|
Quote:
mushboy said: id take them out of the bags for the pic. the rw looks like rw which ime looks iffy
the pesa has bacterial splooge action.
take better pics ya old geezer
LOL
10-4 to the better picts
I'm doing 6 random transfers from each just to see what happens.
|
|