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DNA_Trip
Stranger

Registered: 10/02/19
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Re: Injecting sterile water? [Re: A.k.a]
#26437428 - 01/16/20 07:52 PM (4 years, 1 month ago) |
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Yeah and your popcorn isn’t completely covered and colonized like my grains...
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Elrik
Soulflyer


Registered: 08/29/19
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Re: Injecting sterile water? [Re: DNA_Trip]
#26437635 - 01/16/20 10:46 PM (4 years, 1 month ago) |
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Quote:
DNA_Trip said:I soaked my grains for 24 hours, and then boiled for 10 minutes and let the steam evaporate.
For me this has always been a recipe for too-dry grain. I experimented around a little and the best modification I came up with for wheat is to soak at pasteurization temperature for several hours, bring to a gentle boil and keep it there just until grains start to burst [about 10 minutes, seemingly longer with coffee water], then turn off the heat and let it cool while stirring a few times. I've tried rinsing and drying with a towel and I've tried not doing that, to date not doing it hasn't harmed anything beyond being a little harder to shake once colonized. Cooling in the cooking water will let it suck up lots of water and fully hydrate. 400 grams wheat, 1 gram gypsum, and 4 cups water will leave behind just 1/2 to 1 cup of extra liquid after the cool [which can be fortified with some MEA and used for agar].
Oh, be prepared to use less grain per jar. My 60 gram size jars went down to a maximum of 52.5g and my pint jars went from comfortably holding 125g to barely being shakable at 110g.
-------------------- The Nook
Edited by Elrik (01/16/20 10:48 PM)
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Funguys95
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Re: Injecting sterile water? [Re: DNA_Trip]
#26437740 - 01/17/20 01:24 AM (4 years, 1 month ago) |
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I think some myc just takes longer to grow.. I’ve got 2 jars that have been going at least 3 weeks and still aren’t 100% colonized and the rest all colonized super fast. Ironically the fast myc was in the dryer jars than the other two I have. To answer the original question I would think adding water at this stage would just ad the possibility of contam. This is one of my jars after 3 weeks
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cronicr



Registered: 08/07/11
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Re: Injecting sterile water? [Re: Funguys95]
#26437759 - 01/17/20 01:59 AM (4 years, 1 month ago) |
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U filled them too much your supposed to shake
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,284
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Re: Injecting sterile water? [Re: cronicr]
#26437770 - 01/17/20 02:17 AM (4 years, 1 month ago) |
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I was trying to figure out what was off about it and couldn’t good eye
Faht
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SpunkyMonkey88
Stranger



Registered: 10/08/19
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Re: Injecting sterile water? [Re: cronicr]
#26437878 - 01/17/20 06:06 AM (4 years, 1 month ago) |
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Quote:
Funguys95 said: I think some myc just takes longer to grow.. I’ve got 2 jars that have been going at least 3 weeks and still aren’t 100% colonized and the rest all colonized super fast. Ironically the fast myc was in the dryer jars than the other two I have. To answer the original question I would think adding water at this stage would just ad the possibility of contam. This is one of my jars after 3 weeks

Quote:
cronicr said: U filled them too much your supposed to shake
Ya that jar would be done by now if he could've/would've shook it at 30%
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Funguys95
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I shook a few times but by 65% or so it was just a solid white brick, Almost impossible to break up. And that jar is actually 4 1/2 weeks.. and yeah I never keep in mind how much the grain will rise when it’s cooked..
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Psychgro
Mr. Stranger


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Re: Injecting sterile water? [Re: Funguys95]
#26444683 - 01/21/20 11:24 AM (4 years, 27 days ago) |
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DNA, any updates on your jars/ course of action??
-------------------- Looking to learn all about it
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DNA_Trip
Stranger

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Re: Injecting sterile water? [Re: Psychgro]
#26445606 - 01/22/20 04:24 AM (4 years, 26 days ago) |
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I spawned each jar into their own shoebox 3 days ago, but it doesn’t look like any of them are really taking over and colonizing the substrate. It’s not really proliferating and taking over. My last few always took off within a couple of days devouring the coir with rhizo shooting all through the top of the substrate and you can watch the colonization of the substrate through the sides. Right now the grains are just sitting there, they’re white, but it doesn’t look like any mycelium is spreading or colonizing through...
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trubblesome
Stranger



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Re: Injecting sterile water? [Re: DNA_Trip]
#26445665 - 01/22/20 05:57 AM (4 years, 26 days ago) |
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have you got stuff for agar/started plates yet?
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DNA_Trip
Stranger

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Well I don’t have a laminar flow hood, and still using a SAB can even get contaminated as almost as open air, so I don’t know if it’s worth putting in the time to make some rather than just buying some. I looked some up, and you can already buy a pack of LME plates so I’m thinking about doing that instead.
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A.k.a
Stranger



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Re: Injecting sterile water? [Re: DNA_Trip]
#26445784 - 01/22/20 08:06 AM (4 years, 26 days ago) |
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Dude SAB is more than adequate. It’s your fault if you’re having problems with it, my main thing was remembering not to move anything over something clean.
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LAGM2020     
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trubblesome
Stranger



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Re: Injecting sterile water? [Re: A.k.a]
#26445794 - 01/22/20 08:14 AM (4 years, 26 days ago) |
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you definitely don't need a flow hood. many labs pour plates in open air with proper precautions and good sterile technique. an SAB is more than adequate.
There are also "no-pour" teks where you just mix the agar and nutrients, pour them in to a container that can withstand the pressure cooker and sterilize it right in the dish in the PC.
Here's the classic: Pasty Plates you could use simpler nutrients and agar powder if you want though, you don't have to do the potato flakes/karo etc. you could just do light malt extract and agar powder. people do them at agar 15 g/L and LME from 7.5 g/L up to 15 g/L
but either way, buying them pre made or making them on your own, working with agar is going to make your cultivation much less stressful.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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My how to do agar videos are filmed in a SAB... I did the agar work while talking. If you can't get good results with a SAB a FH will be no improvement since it doesn't fix your technique
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DNA_Trip
Stranger

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Re: Injecting sterile water? [Re: bodhisatta]
#26445977 - 01/22/20 09:51 AM (4 years, 26 days ago) |
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Yeah I’m trying to decide which is the best. There are so many TEKs. Since I’m a beginner I’m trying to find out which one is the easiest and best for me. I was thinking of just buying some already made ones from Amazon. Or would you not recommend that?
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A.k.a
Stranger



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Re: Injecting sterile water? [Re: DNA_Trip]
#26445981 - 01/22/20 09:54 AM (4 years, 26 days ago) |
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All you need is the tub and either a torch and a knife/box cutter or a coffee can you can heat up and push through.
I did mine by getting the box cutter blade red hot and cutting circles out. I could only do like an inch at a time before re torching or the plastic would crack. Still only took like ten mins.
There’s no reason to buy one pre made.
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LAGM2020     
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trubblesome
Stranger



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Re: Injecting sterile water? [Re: A.k.a]
#26445984 - 01/22/20 09:55 AM (4 years, 26 days ago) |
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i think they're talking about pre made plates
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Even worse lol
Premade plates is like buying half ate food. It's ridiculous
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DNA_Trip
Stranger

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Re: Injecting sterile water? [Re: bodhisatta]
#26446005 - 01/22/20 10:01 AM (4 years, 26 days ago) |
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Why is that BOD? To buy pre made agar plates?
Edited by DNA_Trip (01/22/20 10:02 AM)
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,755
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Re: Injecting sterile water? [Re: DNA_Trip]
#26446039 - 01/22/20 10:20 AM (4 years, 26 days ago) |
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Edited by LadysKnight (01/22/20 10:27 AM)
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