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Offlinetedoro
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Registered: 02/06/15
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Re: Best Liquid Inoculant (LI) for Inoculating grains FAST. [Re: Rapjack]
    #26446032 - 01/22/20 10:15 AM (4 years, 7 days ago)

Quote:

Rapjack said:
The surest way to gain speed and performance imo  is to focus on cloning and culture work. Have seen clone grows on this site that went from plant to harvest in only 17 days.




Boy I'm feelin that. I just started anew with spores because of a piggybacking contam and my MS mycelia is slow.

But while that is true, I am looking at my perfectly prepped winter wheat berries in a bag and can't help but think that I can get an agar plate to leap faster than I am currently doing. I wonder if a bit of coffee added to my berries pre-PC'ing could coat the husks with some goodness...


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Offlinespiritlands
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Registered: 06/21/10
Posts: 1,616
Loc: Flag
Last seen: 1 month, 27 days
Re: Best Liquid Inoculant (LI) for Inoculating grains FAST. [Re: Shr00merN00ber]
    #26446229 - 01/22/20 12:02 PM (4 years, 7 days ago)

Quote:

Shr00merN00ber said:
Can I ask, why did you wrap your jar lids? Parafilm?





Call it paranoia/preventative maintenance. Jars come out of the PC and go in the shmuvbox , I work and then seal them in the shmuvbox . When I get ready to use them I peel off the press-n-seal and alcohol wipe around the lid, in the shmuvbox . The lid and seal never gets touched by hands or open air until I break the seal under flow. I handle my jars a lot and I just don't want anything to be able to get in that gap around the lid. Not necessary but I'm having consistent success and doing this isn't going to hurt anything so I'mma keep doing it.


^^These are AA+ started Saturday from one slurried pint jar. Multispore. There's 4 more gallons as well. Started the gallon jar on Friday. My 'jane doe' project, multispore from a pint pf cake. There's six more gallons of 'jane doe' as well.


↑↑The pint with the ? is the one I slurried to 4 gallons plus what you see in the first pic. I just forgot to write the date hence the '?'.


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Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:




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OfflineShr00merN00ber
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Registered: 12/19/18
Posts: 232
Last seen: 6 months, 12 days
Re: Best Liquid Inoculant (LI) for Inoculating grains FAST. [Re: spiritlands]
    #26446424 - 01/22/20 01:40 PM (4 years, 7 days ago)

Thanks for the info and explanation; makes sense.  :nodofunderstanding: Maybe I will adopt the same strategy. Cheers!


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Offlinetedoro
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Registered: 02/06/15
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Re: Best Liquid Inoculant (LI) for Inoculating grains FAST. [Re: Shr00merN00ber]
    #26449675 - 01/24/20 09:55 AM (4 years, 5 days ago)

I was thinking.... what if I left one of my LI syringes at room temp for 3-5 days, then nocced my grains.

pros: my agar might get more gelatinous and stick to the grains more.

pros: The mycelia might grow (sorta like LC does in the broth)

cons: Could get contamed and couldn't see it, and sorta defeats the purpose of LI.


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Extras: Filter Print Post Top
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