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OfflineHoneyBadger9321
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Registered: 08/07/18
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MS to agar - noob question
    #26443293 - 01/20/20 02:47 PM (4 years, 9 days ago)

Its my second attempt at agar. And it is the second time i see no growth or contamination on my plated. I have no idea why. Ive followed the c10 agar TEK.

So my question would be, does my tap water HP levels have anything to do with it.

Secondly, how long does it usually take before you see and growth or contamination.

And thirdly, where do you store your agar (what temp.)?


Cheers


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“Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Heres Tom with the Weather.”
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OfflineKmacmo
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Re: MS to agar - noob question [Re: HoneyBadger9321]
    #26443405 - 01/20/20 04:01 PM (4 years, 9 days ago)

How did you inoculate the plates?

Could try distilled or spring water your PH could be the issue.

Spores can take upto 3 weeks to germinate, contamination has to be introduced it won't just appear.. Bad sterile practise could do that

Anywhere from 18-28c will be fine temp, higher end being preferred for a speedy growth

Can store them anywhere once there sealed, I stack em on a shelf out of direct light


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OfflineHoneyBadger9321
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Re: MS to agar - noob question [Re: Kmacmo]
    #26443453 - 01/20/20 04:37 PM (4 years, 9 days ago)

I've inoculated directly from the MS solution into a syringe and onto the agar. For the agar i used  (per. 500ml: 10g agar-agar, 10g potato dextrose and 3.5g dextrose)...and some food coloring.  Its been 68h since the inoculation, Im keeping them at 20C in the dark.

How much do you think the tap water is a factor? Should i bother testing the PH level, or just switch to distilled?


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“Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Heres Tom with the Weather.”
― Bill Hicks



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OfflineNewbie2626
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Re: MS to agar - noob question [Re: HoneyBadger9321]
    #26444289 - 01/21/20 05:09 AM (4 years, 8 days ago)

If it has only been 68 hours, you are just not being patient. Give it some more time. Spore can germinate in a day or 2 or a week or two depending on the age of the spore etc. I would say if you don't see any growth in 2 weeks, start over. 

I have in all the years not once ph balanced my agar and I haven't had any issues with germination. I don't use straight tap water, I use filtered water, bottled water or distilled water

I keep the room where all my agar plates are at 25C and that seems to be the best in my experience. That is what works for me


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OfflineMr.Wizard
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Re: MS to agar - noob question [Re: Newbie2626]
    #26444544 - 01/21/20 09:51 AM (4 years, 8 days ago)

Quote:

Newbie2626 said:
If it has only been 68 hours, you are just not being patient. Give it some more time. Spore can germinate in a day or 2 or a week or two depending on the age of the spore etc.




I agree - you just need to wait longer. I inoc'd my first 3 plates on the 15th, and 3 more on the 16th and only have growth on 1 out of six. I'm pleased.


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OfflineHoneyBadger9321
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Re: MS to agar - noob question [Re: HoneyBadger9321]
    #26477966 - 02/10/20 04:53 AM (3 years, 11 months ago)

So here is the update after 24 days:

PIC 1:


PIC 2:


PIC 3:


PIC 4:


Thoughts?


--------------------
“Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Heres Tom with the Weather.”
― Bill Hicks



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InvisiblebodhisattaMDiscordReddit
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Re: MS to agar - noob question (moved) [Re: HoneyBadger9321]
    #26478869 - 02/10/20 04:15 PM (3 years, 11 months ago)

This thread was moved from Advanced Mycology.

Reason:
Not advanced


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OfflineKmacmo
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Re: MS to agar - noob question (moved) [Re: bodhisatta]
    #26480399 - 02/11/20 02:55 PM (3 years, 11 months ago)

Pic one looks best take some cuts from that one, pic 3 looks bacterial but I could be wrong Ofcourse


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InvisibleInfiniteDreams
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Registered: 10/25/19
Posts: 1,224
Re: MS to agar - noob question (moved) [Re: Kmacmo]
    #26480723 - 02/11/20 05:41 PM (3 years, 11 months ago)

Seconded, toss all the plates except for the first one.  Take one sample from each of those larger colonies, you could get 6 or 7 total if you want.


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