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Kitestormrising
Christian Funk Dweller


Registered: 12/20/19
Posts: 15
Loc: New Orleans, Louisiana
Last seen: 3 years, 5 months
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Bunch ~O~ Random Questions HELP!
#26443201 - 01/20/20 01:49 PM (4 years, 9 days ago) |
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Hey guys. OK so here is the deal. I am planning on building me a hybrid still air box that is fitting with a HEPA flow hood inside of it. Call it.. the best of both worlds. But wait.. theres more! I want to put 1 or 2 UV-C lights inside of the box.. just shining down on the work area. My first question is this. Does UV-C light harm and or kill spores, mycellium, or even mushrooms.. at all? OR.. would all of those things.. the spores, the Mycellium, and any mushrooms.. be A-OK? I say Mycellium and mushrooms too because I am ALSO intending to hang some UV-C lights inside my grow chamber.. which is probably gonna be of fairly decent size. Like.. 6 and a half feet wide, 4 feet deep, and almost 8 feet tall.. with shelves galore in it that would be holding those large sized aluminum trays. So like.. probably around 40 or so trays.. maybe more.. growing inside of the room I intend to build. I just wanted to make sure that some pretty bright UV-C lights hanging all over the place.. even if they only come on for about 15 minutes at a time every hour or two.. I just wanna make sure that they aren't gonna be killing or even just somehow harming any of my Mycellium and or mushrooms. So!.. what say you guys?
-------------------- ~Donald Trump is the Antichrist that was prophecied to come in the book of Revelation~ (Not joking)
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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I'm not trying to be rude but I only got through about half your post because IME SAB's are very efficient in their own. so any lights, ets are just unnecessary and/or counter productive ...
Ok not what your looking to hear but that's the fact of it... Did like 5 months of agar work and hardly had any plates contaminate while pouring petri's...which tells me the contamination I had on my culture plates was from things other than the air...
I think those UV lights would be bad for the mycelium though I'm not certain...I would just keep it simple man... you'll thank yourself later..
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Don't hybridize shit. Use a SAB or build a flow hood. They aren't interchangeable. Given the resources you are likely willing to use I'd vote flow hood. You will not regret it. SAB works but I'm not even sure I could continue this hobby at this scale if I was using one honestly.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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trubblesome
Stranger



Registered: 11/09/19
Posts: 406
Last seen: 10 months, 17 days
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Re: Bunch ~O~ Random Questions HELP! [Re: Smartattack]
#26443280 - 01/20/20 02:39 PM (4 years, 9 days ago) |
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UV lights at the intensity they would need to be to do anything to kill contaminants would harm you as well.
just keep it simple. flow hoods and SABs work on similar principles but are at odds with each other. I'm not sure why or how you would combine the two.
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Kitestormrising
Christian Funk Dweller


Registered: 12/20/19
Posts: 15
Loc: New Orleans, Louisiana
Last seen: 3 years, 5 months
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Re: Bunch ~O~ Random Questions HELP! [Re: trubblesome]
#26443593 - 01/20/20 05:53 PM (4 years, 9 days ago) |
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OK.. look guys I know OK? I get it. But I ONLY NEED TO KNOW.. will the UV-C harm/kill my MYCELLIUM AND OR MY MUSHROOMS? It isnt gonna be hurting me because I will be shielded. I ONLYYY need to know.. ONLY.. if it can or will harm the MYCELLIUM OR MUSHROOMS. That is ALL. Does anyone know this at all by chance? I am gonna be shielded.. no harm to me. Forget anything and everythinggg else. JUST need to know.. will it hurt or even kill my MYCELLIUM OR MUSHROOMS???
-------------------- ~Donald Trump is the Antichrist that was prophecied to come in the book of Revelation~ (Not joking)
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Sorry but I think all anyone is going to tell you is that this is fail.
We see what you are trying to do here, it is fail.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,274
Loc: where?
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Re: Bunch ~O~ Random Questions HELP! [Re: Smartattack]
#26443631 - 01/20/20 06:13 PM (4 years, 9 days ago) |
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A Sab with a flowhood inside? Worst of both worlds.
Uv lights is extra icing on the noobcake. Wrong tool for the wrong job.
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Bph
Stranger



Registered: 10/11/18
Posts: 1,466
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Re: Bunch ~O~ Random Questions HELP! [Re: mushboy] 1
#26443719 - 01/20/20 07:11 PM (4 years, 9 days ago) |
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Your good. I hear uv increase potancy too so best of three world's
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Barrel
Stranger
Registered: 10/27/18
Posts: 90
Loc: USA
Last seen: 3 years, 8 months
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Re: Bunch ~O~ Random Questions HELP! [Re: Bph]
#26443835 - 01/20/20 08:24 PM (4 years, 9 days ago) |
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Please, take a step back and try to use logic.
A SAB works by keeping air out, and the air that's in it still. A flow hood works by pushing clean air in a straight line away from your work without anything disturbing the flow but your work. How would these work together?
UVGI sterilizes by denaturing the DNA of microorganisms. Mold and yeast, which are fungi, are a common fungi cultivation contaminant. Fungi have DNA. If you're trying to kill contaminant fungi, why wouldn't it kill your cultivated fungi?
You're using trays as a means to isolate groups of fungi. Isolation is one of the best ways to mitigate contamination and cross-contamination. Why would you put isolated groups of fungi into the same grow chamber?
40 trays take 40 times the effort of a single tray. Someone that's experienced in cultivating mushrooms would have a high success rate from experience, making 40 times the effort yield 40 times the results. Someone inexperienced in cultivating mushrooms relies heavily on luck to attain results when they don't study the work of those that are experienced. Why would you try to maximize the probability of failure?
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: Bunch ~O~ Random Questions HELP! [Re: Barrel]
#26443848 - 01/20/20 08:32 PM (4 years, 8 days ago) |
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I feel like you got some good advise, UV Bad...
If you plan on fruiting in aluminium trays though make sure to line them with something. I have had mushrooms eat the trays I used.. There are some pictures in my profile of the aluminum trays and the mushrooms eventually consumed the metal.
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Bph
Stranger



Registered: 10/11/18
Posts: 1,466
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Re: Bunch ~O~ Random Questions HELP! [Re: Sockadin]
#26444281 - 01/21/20 04:53 AM (4 years, 8 days ago) |
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I don't think he really wants advice. I swear sometimes I think people go out of their way to make a post that's controversial just so they can. I don't waste my time giving advise to people who clearly don't want it and are possibly just trolling.
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Kitestormrising
Christian Funk Dweller


Registered: 12/20/19
Posts: 15
Loc: New Orleans, Louisiana
Last seen: 3 years, 5 months
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Re: Bunch ~O~ Random Questions HELP! [Re: Barrel]
#26444397 - 01/21/20 07:31 AM (4 years, 8 days ago) |
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the best advice i got from anyone is this one speaking about how uv can kill the mycellium. THANK YOU! TThats all i wanted to know. kudos to u dude. as far as a flow hood sab goes.. it would work because i would have exaust blowing the bad air out. so therefore.. all that COULD be in there.. is clean air everywhere. This person is the one i was waiting for. THANK U THANK U THANK U
-------------------- ~Donald Trump is the Antichrist that was prophecied to come in the book of Revelation~ (Not joking)
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,268
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Some ppl just gotta learn the hard way best of luck to ya though!
Faht
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trubblesome
Stranger



Registered: 11/09/19
Posts: 406
Last seen: 10 months, 17 days
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Quote:
Kitestormrising said: the best advice i got from anyone is this one speaking about how uv can kill the mycellium. THANK YOU! TThats all i wanted to know. kudos to u dude. as far as a flow hood sab goes.. it would work because i would have exaust blowing the bad air out.
I'm still not sure you're understanding. that's no longer a STILL air box. it's a vertical flow cabinet. They either have a perforated floor so that air can be drawn downward in to a plenum and back up to the top where it's forced through the HEPA filter again, or else it has an even, wide opening so that air flow is even and laminar. here
just blowing air through a hepa filter in to a still air box that has 2 holes for the air to move out of will create turbulent air and make anything that's on your hands or your tools swirl around in the air and land on the shit you're trying to keep clean. all anybody is saying is that it's a lot of extra time money and effort to achieve the same goal that something simpler would.
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,670
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Re: Bunch ~O~ Random Questions HELP! [Re: trubblesome]
#26444456 - 01/21/20 08:43 AM (4 years, 8 days ago) |
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Where would we be without pioneers tho eh? People willing to turn their nose up at solid advice and march headfirst into assured failure. Love to see people try crazy shit at their own expense. Someone will learn something from this.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,274
Loc: where?
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Re: Bunch ~O~ Random Questions HELP! [Re: LadysKnight]
#26444460 - 01/21/20 08:46 AM (4 years, 8 days ago) |
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MrBovineJoni
Glip-Glop

Registered: 11/28/19
Posts: 264
Last seen: 13 days, 13 hours
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Re: Bunch ~O~ Random Questions HELP! [Re: LadysKnight]
#26444495 - 01/21/20 09:17 AM (4 years, 8 days ago) |
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Quote:
LadysKnight said: Where would we be without pioneers tho eh? People willing to turn their nose up at solid advice and march headfirst into assured failure. Love to see people try crazy shit at their own expense. Someone will learn something from this.
There is a big difference between pioneering and ignorance 
When one understands why the process/method X works and then tries to improve it based on evidence and some understanding it is good.
When someone(like OP here) asks for an advice for a very specific thing and most of what he wrote makes people question if he is a troll or genuinely misguided budding mycologist it can get difficult.
It is very clear he/she lacks basic understanding of why some things happen(or are done) certain way and wants to introduce relatively advanced changes and just completely ignores advice from people who have years if not decades of experience over his own misinformed ideas(mixing of SAB and FH principles together gets mentioned at least once a week in this forum by someone new who thinks they know shit but that immediately shows they are complete beginners (or just wrong)).
It is not even clear what he/she wants to change with this "modification"? Will it improve sterile technique? Well a lot of people get it all done with just SABs (FH is kind of for larger scale and/or convenience) - however, there are use cases, where SAB is still preferred over FH.
If there are way more than acceptable contaminants from person's SAB work ..learn to improve the sterile work instead of adding FH and UV lights in a SAB which is wasteful at the best scenario and completely unusable in the worst.
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Re: Bunch ~O~ Random Questions HELP! [Re: MrBovineJoni]
#26444600 - 01/21/20 10:29 AM (4 years, 8 days ago) |
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So the advice you got about your special specific question is taken to your heart. But those who predict all the other fails, your like "nah, they just dicks"?
Should be some dope teks coming from this research.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,670
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Re: Bunch ~O~ Random Questions HELP! [Re: MrBovineJoni]
#26444671 - 01/21/20 11:18 AM (4 years, 8 days ago) |
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Didn't Columbus think he was sailing to India? Bon voyage, young explorer!
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MrBovineJoni
Glip-Glop

Registered: 11/28/19
Posts: 264
Last seen: 13 days, 13 hours
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Re: Bunch ~O~ Random Questions HELP! [Re: LadysKnight]
#26444811 - 01/21/20 12:50 PM (4 years, 8 days ago) |
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Quote:
LadysKnight said: Didn't Columbus think he was sailing to India? Bon voyage, young explorer!
yes, but he knew how boats/ships work and had many sea voyages before he rediscovered North America
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