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OfflinePrietenul
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Registered: 11/22/19
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LC from antibiotic agar / antibiotic agar to grains?
    #26443132 - 01/20/20 12:55 PM (4 years, 9 days ago)

Hi,

I have streaked some cube spores to antibiotic agar. According to the vendor the antibiotic is an Aminoglycoside, so I suspect it's Gentamicin. (It's PC resistant)

Can the myc from those plates be used to inoculate grain jars or LC directly or should I transfer to non-antibiotic plates first?

If it's a problem, would the "puncture LC method" (i.e. poking the myc with a syringe and squirting into LC jar) be a viable option? I mean, it would be only a minuscule amount of agar that lands in the broth.


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