|
superwhitedolemite
Coincidentia oppositorum



Registered: 10/30/19
Posts: 50
Last seen: 3 years, 11 months
|
Re: Agar Spore Transfer: How am I doing? [Re: A.k.a]
#26441418 - 01/19/20 09:45 AM (4 years, 29 days ago) |
|
|
Man, you're killin' it! I want to have all of these problems. Haha!
I'll go with shoeboxes then - what you're saying makes perfect sense. As I up my skills, maybe then move to a bigger tub.
rido, thanks for the link!
I've updated the OP with much better pictures. Would appreciate feedback from those that recommended I do this. Thanks!
Here are some follow-ups from this morning as well. I'm watching them change by the hour!
I rolled the dice to see what would happen. I carved up the cleanest looking plate - gen 1, no transfer - and threw the colonized agar into a few rye grain jars I prepped the other night. Two days in and it's actually looking pretty solid, no?
Thanks again, all.
|
jbgtaa
extraterrestrial



Registered: 06/09/19
Posts: 1,785
Last seen: 3 years, 3 months
|
|
Thankfully aka hopped in here...
All of those plates look fine but need more transfers. You’re not going to get canopies from any culture like that unless you have extremely optimal surface conditions. Furthermore, don’t waste too much time on MS (multispore cultures), just take a few edges, that look similar, grain them, and then spawn it all andfind a good clone. Clusters are always good clones. Then your working with some predictable and real.
-------------------- If the thunder don't get ya, the lightning will. In another time's forgotten space, your eyes looked through your mother's face. Trade List Forever giving away prints. PM at anytime for a free print.
|
trubblesome
Stranger



Registered: 11/09/19
Posts: 406
Last seen: 11 months, 5 days
|
|
just keep a close eye on these. ten transfers is a lot but at least one is recommended. show us what tubs you have and we can help you find a tek that more closely matches them.
--------------------
|
superwhitedolemite
Coincidentia oppositorum



Registered: 10/30/19
Posts: 50
Last seen: 3 years, 11 months
|
Re: Agar Spore Transfer: How am I doing? [Re: trubblesome]
#26441447 - 01/19/20 10:02 AM (4 years, 29 days ago) |
|
|
Quote:
jbgtaa said: Thankfully aka hopped in here...
All of those plates look fine but need more transfers. You’re not going to get canopies from any culture like that unless you have extremely optimal surface conditions. Furthermore, don’t waste too much time on MS (multispore cultures), just take a few edges, that look similar, grain them, and then spawn it all andfind a good clone. Clusters are always good clones. Then your working with some predictable and real.
Interesting. So, is it the concentration of the mycelium, or the robustness/health, that makes it more viable on each transfer? I noticed the first transfers I did are much denser and growing in a radial pattern.
Like I said, I just whipped up 3 jars and threw some in there, just to see what would happen. I'll stick these in 3 small tubs when they're ready and see how it goes. Strictly as an experiment to satisfy my curiosity.
I often like to learn things the hard way as well. Life-long affliction.
Quote:
trubblesome said: just keep a close eye on these. ten transfers is a lot but at least one is recommended. show us what tubs you have and we can help you find a tek that more closely matches them.
Fair enough. No tubs yet, this will be my first run. Will definitely throw a thread out there once they're started. All I've succeeded in doing to date, is 11 cakes a la PF Tek. That's where all the spores for this new round of stuff came from.
Edited by superwhitedolemite (01/19/20 10:03 AM)
|
jbgtaa
extraterrestrial



Registered: 06/09/19
Posts: 1,785
Last seen: 3 years, 3 months
|
|
I really can’t speak on isolating/culturing multispore cultures. I find it to be a waste of time and the returns are usually diminishing considering you have no clue what you’re working with.
When you take a clone, you’ll see what I’m talking about. Similar rhizomorphs, radial patterning, less “piled” mycelium. Check my gallery there’s some solid GT clone cultures, and some not so solid, but youll see how it differs from MS.
-------------------- If the thunder don't get ya, the lightning will. In another time's forgotten space, your eyes looked through your mother's face. Trade List Forever giving away prints. PM at anytime for a free print.
|
trubblesome
Stranger



Registered: 11/09/19
Posts: 406
Last seen: 11 months, 5 days
|
|
Quote:
superwhitedolemite said:
My thoughts exactly - a tub is just a big cake. I'm only producing for my own use, so I don't need massive yields and giant grows. I bought a bunch of small tubs - larger than shoeboxes but not by much. Pure part-time hobbyist here.
these tubs
you should keep it all in one thread (this one) instead of starting a new one. so many people reference previous threads theyve made about their projects and assume everyone knows everything theyve done up to that point in their grow and can tell them exactly why this or that is happening. keeping it in one thread will make it easier for people to help pinpoint problems down the road
--------------------
|
superwhitedolemite
Coincidentia oppositorum



Registered: 10/30/19
Posts: 50
Last seen: 3 years, 11 months
|
Re: Agar Spore Transfer: How am I doing? [Re: jbgtaa]
#26441472 - 01/19/20 10:17 AM (4 years, 29 days ago) |
|
|
Quote:
jbgtaa said: I really can’t speak on isolating/culturing multispore cultures. I find it to be a waste of time and the returns are usually diminishing considering you have no clue what you’re working with.
When you take a clone, you’ll see what I’m talking about. Similar rhizomorphs, radial patterning, less “piled” mycelium. Check my gallery there’s some solid GT clone cultures, and some not so solid, but youll see how it differs from MS.
Sorry, relatively new to all the terminology. That is to say; multispore culture is the result of transferring spores to agar?
I've gotten a lot of comments about how ugly my plates are, so I'm trying to ascertain if that's just preference or if the end result is really radically different. I'm very new, so I'm at the stage where if I get anything to grow and it's not contaminated, I'm super stoked about it. Haha.
Will check out your stuff. I've made one cloning attempt so far that went hilariously wrong - 100% bacterial shitstorm. The fatal mistake was cutting into the mushroom and using the same blade to immediately transfer the sample. I'm definitely going to give it another try on the next flush.
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 1 hour, 55 minutes
|
|
I had trouble with bacteria my first few clones also.
Gotta glove up then rip the stem in half in the SAB and make sure the blade doesn’t touch the outside of the stem.
My method at this point is to cut a square shape pretty deep in the center tissue then cut the square in half diagonally downward so a nice little triangle shape comes loose.
I also accidentally discovered the tiniest piece of clone tissue will grow fine. I had a little speck fall off onto a plate and it grew out same as the actual transfer.
--------------------
LAGM2020     
|
superwhitedolemite
Coincidentia oppositorum



Registered: 10/30/19
Posts: 50
Last seen: 3 years, 11 months
|
Re: Agar Spore Transfer: How am I doing? [Re: A.k.a]
#26441488 - 01/19/20 10:36 AM (4 years, 29 days ago) |
|
|
Quote:
A.k.a said: I had trouble with bacteria my first few clones also.
Gotta glove up then rip the stem in half in the SAB and make sure the blade doesn’t touch the outside of the stem.
My method at this point is to cut a square shape pretty deep in the center tissue then cut the square in half diagonally downward so a nice little triangle shape comes loose.
I also accidentally discovered the tiniest piece of clone tissue will grow fine. I had a little speck fall off onto a plate and it grew out same as the actual transfer.
Noted, thanks! Yeah, we were laughing about that. All that prep, cleaning, etc. and my fatal flaw was cutting into the stem, then cutting the sample out. Everything I did from that moment forward was a waste of time and money. Lol!
Admittedly, I got a little cocky after zero contam on my first attempt with PF, and assumed I was invincible. I took nearly zero precautions on that grow and didn't even have a SAB. I got the impression at that point, that this stuff would be easy.
|
trubblesome
Stranger



Registered: 11/09/19
Posts: 406
Last seen: 11 months, 5 days
|
|
i mean, you can use the same blade, just flame sterilize it
--------------------
|
superwhitedolemite
Coincidentia oppositorum



Registered: 10/30/19
Posts: 50
Last seen: 3 years, 11 months
|
Re: Agar Spore Transfer: How am I doing? [Re: trubblesome]
#26442657 - 01/20/20 05:56 AM (4 years, 28 days ago) |
|
|
Right, yeah. That's the one thing I didn't do. Makes sense but the video I followed didn't, so I thought nothing of it.
|
LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,755
|
|
Quote:
superwhitedolemite said: Right, yeah. That's the one thing I didn't do. Makes sense but the video I followed didn't, so I thought nothing of it.
Also, like aka said, tear it open, don't cut into it. When you cut in from the outside you are pushing contams into the inside.
|
superwhitedolemite
Coincidentia oppositorum



Registered: 10/30/19
Posts: 50
Last seen: 3 years, 11 months
|
Re: Agar Spore Transfer: How am I doing? [Re: LadysKnight]
#26449897 - 01/24/20 12:21 PM (4 years, 24 days ago) |
|
|
Right, gotcha. I suppose it'd be just as easy to push contams into the flesh of the stem by ripping it open, without being super careful?
Excited to try cloning again now that I've had more success w/ agar plates. I'll study up before the next attempt. Thanks!
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 1 hour, 55 minutes
|
|
As long as you tear it it should be clean. Cutting it you push the blade through the dirty skin and slide it through the clean stuff, which could spread bacteria kind of like butter onto bread.
I pinch the base of the stem to crack it then pull it apart like string cheese, so I’m only ever touching the outside of the base of the stem, then take the sample from the middle/top part. The thicker they are the easier it is to cut a clean piece out.
--------------------
LAGM2020     
Edited by A.k.a (01/24/20 12:32 PM)
|
superwhitedolemite
Coincidentia oppositorum



Registered: 10/30/19
Posts: 50
Last seen: 3 years, 11 months
|
Re: Agar Spore Transfer: How am I doing? [Re: A.k.a]
#26449950 - 01/24/20 12:43 PM (4 years, 24 days ago) |
|
|
Round of pics from yesterday. Wish I had time to get more today since everything is so much further along! Bumped the temp in my room the last two days and man, what a difference!
I circled some potential trouble spots. Newer plates (round 2 transfer) look much better so may just ditch the first round. Maybe that's not mold but why risk it, right?
Jars are going nuts. I think I'm maybe 2-3 days away from spawning to tubs. Shook em up once already and they might need it again today.
Some questions I asked initially but probably got lost in the shuffle:
What do I do with plates that I can't use right now? I have more healthy agar plates than I'm going to be able to utilize. Can they be safely refrigerated? Will the mycelium die? If they can be stored, for how long?
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 1 hour, 55 minutes
|
|
Are those the jars you’re talking about?
You can store plates at room temp for months, but I don’t think you’ll get much use out of those. Did you pour that agar or is it premade?
None of the plates look normal but it kind of seems like something going on with the agar.
--------------------
LAGM2020     
Edited by A.k.a (01/24/20 01:27 PM)
|
superwhitedolemite
Coincidentia oppositorum



Registered: 10/30/19
Posts: 50
Last seen: 3 years, 11 months
|
Re: Agar Spore Transfer: How am I doing? [Re: A.k.a]
#26450054 - 01/24/20 01:41 PM (4 years, 24 days ago) |
|
|
I made the agar - was my first attempt. Yes, these jars are also my first attempt at those.
I mentioned earlier on that I had transferred to these jars directly from a first-gen plate, no transfers. They seem to be doing well! If they don't, no worries, it was only an experiment.
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 1 hour, 55 minutes
|
|
Right on.
Well unless they were shaken within a day or so of the pictures they still have a lot of colonizing to do, gotta be solid white.
What was your agar recipe? I’ve been making very soft plates lately and it seems to be affecting the growth now, I’m getting a lot of clear growth at the edges kind of like the myc is growing in the agar rather than on it.
Might be something similar going on with yours.
--------------------
LAGM2020     
|
superwhitedolemite
Coincidentia oppositorum



Registered: 10/30/19
Posts: 50
Last seen: 3 years, 11 months
|
Re: Agar Spore Transfer: How am I doing? [Re: A.k.a]
#26450411 - 01/24/20 05:42 PM (4 years, 24 days ago) |
|
|
Oh, for sure. These pics are from yesterday. As of today, about 50% of the rye is visibly covered again. It seems to really be picking up all of a sudden.
It’s definitely weird how the myc seems to dig into the agar, then sprout up and move outward again. I hadn’t even considered it might be the recipe. I followed this one to the letter:
What’s your preferred recipe and approach?
|
|