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Offlineogdoadtoad
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Registered: 12/04/18
Posts: 9
Last seen: 3 years, 9 months
Agar Assistance.
    #26439961 - 01/18/20 10:05 AM (4 years, 11 days ago)

Hi could anyone help me ID these plates? I did a spore swab a few weeks ago and i think that I could transfer them soon but I would like a second opinion. Thanks !
Plate 1:





Plate 2:





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OfflineGrowtech
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Registered: 11/09/19
Posts: 165
Last seen: 2 years, 5 months
Re: Agar Assistance. [Re: ogdoadtoad]
    #26440008 - 01/18/20 10:49 AM (4 years, 11 days ago)

Looks like a crazy amount of bacteria to me.  But you could probably get something viable off the 3rd plate after a few more transfers.


Edited by Growtech (01/18/20 10:50 AM)


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Offlineogdoadtoad
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Registered: 12/04/18
Posts: 9
Last seen: 3 years, 9 months
Re: Agar Assistance. [Re: Growtech]
    #26440025 - 01/18/20 11:01 AM (4 years, 11 days ago)

Does the middle in the plates look okay for transfer?

Edit: The Orange/yellow is most probably the agar itself if by bacteria you mean that part of the plate. It's been there from the start and hasn't spread.


Edited by ogdoadtoad (01/18/20 11:12 AM)


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OfflineA.k.aM
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Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 7 hours, 28 minutes
Trusted Cultivator
Re: Agar Assistance. [Re: ogdoadtoad]
    #26440052 - 01/18/20 11:16 AM (4 years, 11 days ago)

Dude...



Ok I’m confused did you put like an agar paste in there instead of pouring?

The second picture of plate one there’s a small amount of clean looking myc poking out.

I thought the orange stuff was all bacteria I’ve never seen a plate made like yours.


--------------------
LAGM2020


Edited by A.k.a (01/18/20 11:19 AM)


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Offlineogdoadtoad
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Registered: 12/04/18
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Last seen: 3 years, 9 months
Re: Agar Assistance. [Re: A.k.a]
    #26440097 - 01/18/20 11:41 AM (4 years, 11 days ago)

The plates (potato dextrose) were ordered online, they were advertised as sterile and the seller had a good score. I have no idea how they were made, initially the agar before inoculation was cloudy white with a slight tint but after one week it turned orange. I had bacteria on my first attempt and it definitely didn't look anything like this. Those plates from the first attempts had actual colonies of bacteria (orange and yellow dots and circles)


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OfflineGrowtech
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Registered: 11/09/19
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Last seen: 2 years, 5 months
Re: Agar Assistance. [Re: ogdoadtoad]
    #26440107 - 01/18/20 11:49 AM (4 years, 11 days ago)

So you bought sterile pre-poured agar plates that the seller didn't even bother to pour the whole plate?  Do you by chance have any more of those dishes you could post a picture of before you did anything with them (as they came from the seller)?

Just trying to figure out if they look that gross because they came that way, or if it's contamination.  Definitely not what I would expect from someone making a business out of selling pre-poured agar dishes.


--------------------
Mycolo.gyGenetics & yield tracking system


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Invisiblefilthyknees
no coincidence
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Posts: 6,283
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Re: Agar Assistance. [Re: Growtech]
    #26440113 - 01/18/20 11:52 AM (4 years, 11 days ago)

Did you wrap your plates after swabbing them?


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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InvisibleSmartattack
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Re: Agar Assistance. [Re: filthyknees]
    #26440121 - 01/18/20 11:59 AM (4 years, 11 days ago)

Wtf  :eek: :eek:


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


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Offlineogdoadtoad
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Registered: 12/04/18
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Re: Agar Assistance. [Re: filthyknees]
    #26440148 - 01/18/20 12:21 PM (4 years, 11 days ago)

Quote:

Growtech said:
So you bought sterile pre-poured agar plates that the seller didn't even bother to pour the whole plate?  Do you by chance have any more of those dishes you could post a picture of before you did anything with them (as they came from the seller)?

Just trying to figure out if they look that gross because they came that way, or if it's contamination.  Definitely not what I would expect from someone making a business out of selling pre-poured agar dishes.




Yes i have some more plates which i keep refrigerated since they arrived. Here are two




Quote:

filthyknees said:
Did you wrap your plates after swabbing them?



Yep, i did.


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InvisibleLadysKnight
Hello Ladies
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Registered: 10/09/15
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Re: Agar Assistance. [Re: Smartattack]
    #26440157 - 01/18/20 12:26 PM (4 years, 11 days ago)

Yeah those come complete with bacteria from the seller.
You should make your own plates.
Is your SAB pink?
Is that perlite in the bottom?


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Invisiblefilthyknees
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Registered: 03/08/13
Posts: 6,283
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Re: Agar Assistance. [Re: LadysKnight]
    #26440175 - 01/18/20 12:38 PM (4 years, 11 days ago)

It's a start all over moment. YouTube 'lets grow mushrooms agar' and consider DIY.

Folks here are happy to help with agar, and pm me if you would like fresh prints.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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