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ogdoadtoad
Stranger
Registered: 12/04/18
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Last seen: 3 years, 9 months
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Agar Assistance.
#26439961 - 01/18/20 10:05 AM (4 years, 11 days ago) |
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Hi could anyone help me ID these plates? I did a spore swab a few weeks ago and i think that I could transfer them soon but I would like a second opinion. Thanks ! Plate 1:


Plate 2:

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Growtech
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Registered: 11/09/19
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Looks like a crazy amount of bacteria to me. But you could probably get something viable off the 3rd plate after a few more transfers.
Edited by Growtech (01/18/20 10:50 AM)
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ogdoadtoad
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Re: Agar Assistance. [Re: Growtech]
#26440025 - 01/18/20 11:01 AM (4 years, 11 days ago) |
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Does the middle in the plates look okay for transfer?
Edit: The Orange/yellow is most probably the agar itself if by bacteria you mean that part of the plate. It's been there from the start and hasn't spread.
Edited by ogdoadtoad (01/18/20 11:12 AM)
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 7 hours, 28 minutes
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Dude...
Ok I’m confused did you put like an agar paste in there instead of pouring?
The second picture of plate one there’s a small amount of clean looking myc poking out.
I thought the orange stuff was all bacteria I’ve never seen a plate made like yours.
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LAGM2020     
Edited by A.k.a (01/18/20 11:19 AM)
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ogdoadtoad
Stranger

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Last seen: 3 years, 9 months
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Re: Agar Assistance. [Re: A.k.a]
#26440097 - 01/18/20 11:41 AM (4 years, 11 days ago) |
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The plates (potato dextrose) were ordered online, they were advertised as sterile and the seller had a good score. I have no idea how they were made, initially the agar before inoculation was cloudy white with a slight tint but after one week it turned orange. I had bacteria on my first attempt and it definitely didn't look anything like this. Those plates from the first attempts had actual colonies of bacteria (orange and yellow dots and circles)
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Growtech
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Registered: 11/09/19
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Last seen: 2 years, 5 months
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So you bought sterile pre-poured agar plates that the seller didn't even bother to pour the whole plate? Do you by chance have any more of those dishes you could post a picture of before you did anything with them (as they came from the seller)?
Just trying to figure out if they look that gross because they came that way, or if it's contamination. Definitely not what I would expect from someone making a business out of selling pre-poured agar dishes.
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Agar Assistance. [Re: Growtech]
#26440113 - 01/18/20 11:52 AM (4 years, 11 days ago) |
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Did you wrap your plates after swabbing them?
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Wtf
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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ogdoadtoad
Stranger

Registered: 12/04/18
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Last seen: 3 years, 9 months
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Quote:
Growtech said: So you bought sterile pre-poured agar plates that the seller didn't even bother to pour the whole plate? Do you by chance have any more of those dishes you could post a picture of before you did anything with them (as they came from the seller)?
Just trying to figure out if they look that gross because they came that way, or if it's contamination. Definitely not what I would expect from someone making a business out of selling pre-poured agar dishes.
Yes i have some more plates which i keep refrigerated since they arrived. Here are two


Quote:
filthyknees said: Did you wrap your plates after swabbing them?
Yep, i did.
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,670
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Yeah those come complete with bacteria from the seller. You should make your own plates. Is your SAB pink? Is that perlite in the bottom?
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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It's a start all over moment. YouTube 'lets grow mushrooms agar' and consider DIY.
Folks here are happy to help with agar, and pm me if you would like fresh prints.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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