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Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag

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OfflineSynKyd
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Re: EAT LIQUID INOCULATES™ [Re: Hillbillyjohn]
    #26438625 - 01/17/20 01:45 PM (4 years, 1 month ago)

Liking this idea, just have to ask have you tried doing larger grain jars?  Seems with this speed you could use the half gallon jars without stalling out.


Quote:

eatyualive said:
I’d smash myself in the face if I had to cut 20 wedges for a2g. Liquids I pour like a beer and it takes me about 10 minutes for 30 quarts.




I pour beer in my mouth so.........

Quote:

Hillbillyjohn said:
Damn I read the title and thought I was gonna get to see some fucktard chugging a jug of lc haha.
.




Same, same! 

:cheers:


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: SynKyd]
    #26438667 - 01/17/20 02:10 PM (4 years, 1 month ago)

So here is my trial with the blender lid above. This is extremely low and slow leap off than I’m used to. Normally my jar would be 70-100% colonized by day 8.




What agar recipe are you using? I sincerely think leap off has more to do with texture and thickness of your inoculant than it does the actual amount of mycelia. For instance with pf jar slurries, the inoculant is so thick it literally sticks to every piece of grain after you shake it. So the jars colonize in 3-4 days to 100% with less than 1 cc of inoculant. Whereas you see in my example above I used a standard 1/3 of a regular petri plate and the leap off was slower. Granted I did use less inoculant due to this lid having leftovers and not allowing me to use 10% volume of the inoculant.

In addition, cornmeal plates are 2nd fastest to brf slurries. They are very thick and muddy when poured and it sticks to the grain better. You have Lcs which may slip past the grain and not catch as well.

I used pasty plates in the op. Even with the almost 90% colonized plate, it is still about the same size of mycelia I’m using from 1/3 of a Normal petri plate since they are much larger. The pasty recipe is potato flakes, karo syrup and agar. Sugar definitely helps in leap off. And I have to say, I used to use popcorn grain and leap off was tremendous. I highly believe the difference you are seeing may have something to do with starches and sugars. But let me know what your using?

Another big tip is shaking the jars for at least one minute minimum to distribute the inoculant even. I have jars that take much longer in my batches when I don’t shake them initially as even. Technique is key.

I’m working on some videos or gifs of the shake and pour so you can clarify what I am doing.


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EAT GETS SHIT DONE


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Edited by eatyualive (01/17/20 02:27 PM)


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Offlinetedoro
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26438732 - 01/17/20 03:05 PM (4 years, 1 month ago)



I use 11g to 400 ml water of this malt extract agar. Its what I came up with for just soft enough for LI and some more aggressive growth.

I rather like standard plates, and would like to continue using them if possible.... can I learn to use cornmeal plates with standard plates? Would that be a good move?


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26438740 - 01/17/20 03:11 PM (4 years, 1 month ago)

Nah just use that. Add a little karo syrup to your mix specifically for plates you are using for LI and see if that helps.

Also I used to use popcorn grain as spawn. It has a lot of extra starches on the grain compared to most. But compared to wbs and oats it has much faster leap off from standard g2g.

Are you free pouring?

Also another thing, in jars I feel it’s easier to shake and get even distribution of your inoculant after a shake compared to a bag. If that makes any sense.


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Offlinetedoro
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26438744 - 01/17/20 03:14 PM (4 years, 1 month ago)

If I make 400ml batches, where would you start at with the amount of Karo? and what would be things to look out for? Contams if too much? No improvement if too little?


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Offlinetedoro
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26438752 - 01/17/20 03:17 PM (4 years, 1 month ago)

I'm also wondering if during my syringe noc'ing, if I couldn't squirt it around more evenly, assisting in the mixup.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26438755 - 01/17/20 03:18 PM (4 years, 1 month ago)

So I generally use the standard pasty recipe of 1 cup water to 2 tablespoons agar and 2 teaspoons of potato flakes. I just eye one big glob of karo syrup in that mix. This makes 8 plates.

Maybe change up your recipe only for what you are liquid inoculating to see if there is a noticeable difference. This seems to work really well for me. You could probably add a big glob squeeze out of the bottle to that volume.

Are you using the syringe to inject into bags? I think it may also be that you are using bags and I’m using jars. So when I shake really well, my Inoculant is touching every piece of grain with a good shake.


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Offlinetedoro
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26438763 - 01/17/20 03:22 PM (4 years, 1 month ago)

Will do. I'll report back. This is exciting to think I could get my leapoff to look half as good as yours. Doing a ton of agar work on Sunday.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Offlinetedoro
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26438771 - 01/17/20 03:28 PM (4 years, 1 month ago)

Eats,

I just did some rough measurements and it looks like a pastyplate has over an ounce of agar where my plates I pour as thin as possible... maybe a 1/3 of an ounce. Maybe I'd be better served pouring them thicker so my LI is thicker. I make 20 plates with that 400ml.


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--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Edited by tedoro (01/17/20 03:30 PM)


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26438809 - 01/17/20 03:50 PM (4 years, 1 month ago)

Yes I would agree to try that as well. The agar is sticky and helps the mycelia kinda catch on to the grain. My LI looks like chunky soup when I pour it compared to a plate I used with a standard agar plate. The thicker the better. Take a look.

This is a pasty plate blended to LI.



This is a cornmeal plate LI



This is a pf jar slurry. As you can see it’s solid like mud and coats all the grain upon inoculation. This means it has far more inoculation points thus colonizes twice as fast.



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EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


Edited by eatyualive (01/17/20 03:58 PM)


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Offlinetedoro
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26438815 - 01/17/20 03:53 PM (4 years, 1 month ago)

This is great. I have something to aim for. Many thanks for the time.


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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OfflineA.k.aM
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26438908 - 01/17/20 05:00 PM (4 years, 1 month ago)

Will any oster base work or is it a certain model?

Cutting colonization time by like 2/3s with this rig might be the greatest cult tool ever.


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LAGM2020


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
    #26438918 - 01/17/20 05:05 PM (4 years, 1 month ago)

Yes any stainless steel oster blender base should work unless its some fancy smoothie kinda thing. Dont pc the black plastic bottom piece because it will melt. I used to simply wipe it with iso and use the regular lid and free pour. Then when i was on this forum, i was told it was bad technique. Seemed to work for me for years. But the gasket can get tangled up after a blend so the pour lid is a cleaner and an easier way of pouring and evenly distributing your LI to jars or bags.


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EAT GETS SHIT DONE


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26440401 - 01/18/20 03:05 PM (4 years, 30 days ago)

For reference. This is day 9 on both sets of jars side by side.

Stropharia: normal plate blended LI. I dont know the agar mix but it isnt the same as the mix below. Im also trying this new lid that doesnt dispense as much liquid when pouring. I had to shake these jars to speed them up.



Stropharia: pasty plate blended LI. Normal pouring lid in op. You can see the jars above that were shaken in this pic on the left.


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EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


Edited by eatyualive (01/18/20 03:36 PM)


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Offlinetedoro
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26440494 - 01/18/20 04:26 PM (4 years, 30 days ago)



Excited to make a bit sweeter of a leapoff LI stew. Gunna mark the sweetened malt agar with a color.


--------------------
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
    #26445196 - 01/21/20 08:32 PM (4 years, 26 days ago)

Im updating the op with some pour test gifs. This is the pour test with the spout flush with the bottom of the pour lid.



Also updated this with some gifs of the inoculation, lid swap, and swirl technique.

8) With my right hand, I lift the pour lid and with my left hand I lift the blender jar lid. I swap them at the exact same time so exposure is minimal. Now your jars are ready to inoculate. This is a quick time lapse of the lid swap.(im using my new LI pour lids see below.)



9) I then load my grain jars into the SAB. I wipe each one down all over with an iso paper towel before it is placed into the SAB. I loosen each lid by hand. I let the air settle prior to doing any work.



12) Remove the plug before the pour. I Lightly swirl the liquid inoculate jar before each pour as the mycelia slurry settles. With my left hand I lift the grain jar lid, with my right hand I do a quick turn for a split second and then close the grain jar lid. While I do all this, the grain jar lid is held directly above the grain jar opening to act as a shield from anything falling from above. Grain exposure is minimal during the pour and basically the pour is done underneath the grain jar lid. With these new lids sheon it has a small latch that closes between each pour for convenience. There is about 1cc-3cc volume distributed into each jar from the quick  flick of the wrist. Put the lid back on loosely. We will tighten all the lids at the end.

Swirl technique

Inoculation


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EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


Edited by eatyualive (01/22/20 05:55 AM)


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InvisiblefahtsterM
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26445444 - 01/22/20 12:00 AM (4 years, 26 days ago)

Loving the gifs!

Faht


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Re: EAT LIQUID INOCULATES™ [Re: fahtster]
    #26445454 - 01/22/20 12:14 AM (4 years, 26 days ago)

:takingnotes:


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LAGM2020


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Invisibleeatyualive
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Re: EAT LIQUID INOCULATES™ [Re: staytrippy420]
    #26445664 - 01/22/20 05:56 AM (4 years, 26 days ago)

Yeah i have an entire video of the cut, drop and inoculation but i dont wanna deal with youtbe ect. They are far too long to make 100 gifs lol


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Offlinetedoro
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
    #26455719 - 01/27/20 06:17 PM (4 years, 21 days ago)



Noc'd up eight wheat bags today with thickly poured, karo sweetened soft agar I blended and sucked up in a syringe. This is exciting. 45cc in each. some with 1/2 plate, some with a whole plate. My last bags with thin, no karo plates colonized in 8 days.

You can see the chunks of agar on the berries. I was able to suck up almost all of the broth.

I mixed the bags for 2 whole minutes each. taped the heat sealed corners to avoid a tear.


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Edited by tedoro (01/27/20 06:23 PM)


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