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InvisibleSpunkyMonkey88
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Re: Injecting sterile water? [Re: DNA_Trip]
    #26437000 - 01/16/20 03:22 PM (4 years, 1 month ago)

Quote:

DNA_Trip said:
I don't even know where to start with agar or how to do it. Did you just buy agar Petri dishes?




Only if u want to spend WAY more money... u can make them cheap as hell and it sounds intimidating but it's actually easy AF  to pour your own plates...

If u got a SAB then all u need is petris and agar and ull be set to make much healthier cultures and you can start cloning... or if u wanna do it now go get some glad mini round containers and make some no pours... if u have agar all u need is a few bucks worth of stuff at walmart...


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InvisibleSpunkyMonkey88
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Re: Injecting sterile water? [Re: cronicr]
    #26437003 - 01/16/20 03:23 PM (4 years, 1 month ago)

Quote:

cronicr said:
So now your just gonna tell him to spawn jars at 50% colonization...good job, there  you go u got the answer you wanted to hear, good luck :leaving:




If u r referring to something I said... that is definitely not what I meant... obviously should never spawn anything that isn't 100% just telling him bacterial jars can still produce...once fully colonized


Edited by SpunkyMonkey88 (01/16/20 03:24 PM)


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OfflineA.k.aM
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Re: Injecting sterile water? [Re: DNA_Trip]
    #26437004 - 01/16/20 03:24 PM (4 years, 1 month ago)

To start agar I bought a tub for SAB, a small bad of premixed lme agar, and two sleeves of plates.
And a media bottle, but you can use other stuff instead.

Everything prob cost like 40 dollars. Then once I knew I was into it bought a case of 500 and bigger bags of lme and agar so I could mix my own ratios.


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OfflineDNA_Trip
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Re: Injecting sterile water? [Re: cronicr]
    #26437050 - 01/16/20 03:48 PM (4 years, 1 month ago)

No, I'm very reluctant in doing so, I wasn't planning to. My main concern is harm and what can happen. The last several people have told me I'm being to paranoid and to just do it. I'm still weary and cautious about it, but they're just giving me their experiences is all, they're not doing anything wrong...

P.S. I know you're still referring to my picture of my jars from a couple days ago, they are way more complete than that, but not for certain it's a 100% colonized like my last run.


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OfflineA.k.aM
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Re: Injecting sterile water? [Re: DNA_Trip]
    #26437056 - 01/16/20 03:55 PM (4 years, 1 month ago)

There’s very few things that could grow that can hurt you, and they’re rare and if they did pop up easy to see.

I looked into it a lot before I started too and it seems like you’d have to go out of your way to get sick.

As long as you’re not eating or inhaling large amounts of weird looking mold you’re good.

Plus the dehydrator will take care of pretty much anything in there even if you somehow missed a large patch of mold on a mushroom.


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OfflineDNA_Trip
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Re: Injecting sterile water? [Re: SpunkyMonkey88]
    #26437071 - 01/16/20 04:03 PM (4 years, 1 month ago)

This is how my jars came out this time for some reason. I originally had 3, opened one up last night to take a smell, and it smelled like fresh mushrooms. But this is what brought me to creating this thread because I didn’t understand why they’re like this this time.



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OfflineA.k.aM
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Re: Injecting sterile water? [Re: DNA_Trip]
    #26437084 - 01/16/20 04:08 PM (4 years, 1 month ago)

If you only opened it real quick to smell it it’s probably not any worse off.

I feel you man I never really bothered learning to ID spawn problems cuz I only did syringe to grain grows but now that I’m doing agar I’m working on it.

I still have trouble picking bacteria out but the easiest way for me so far is to look for bald spots on the grains. You can usually see where it looks like sometime is stopping the myc from growing any further on the edges of those spots.


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OfflineDNA_Trip
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Re: Injecting sterile water? [Re: A.k.a]
    #26437096 - 01/16/20 04:16 PM (4 years, 1 month ago)

Well I chunked it though after opening it up. Yeah that is how some of it looks. I did my shake last week, and all of a sudden colonization was looking like this. I thought maybe the grains were too dry or something, but it's just not getting there all the way. Did this ever happen to you in any of your syringe to grain grows? Also, where did you get your agar? I need to start off simple since I don't know anything about it. Just looking it up there are so many different teks for different things.


Edited by DNA_Trip (01/16/20 04:20 PM)


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OfflineA.k.aM
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Re: Injecting sterile water? [Re: DNA_Trip]
    #26437106 - 01/16/20 04:20 PM (4 years, 1 month ago)

I alway use wbs, I definitely had some sketchy jars but never lost one. I did have some that grew normally until I shook them and then they took forever to recover, and when they did the myc wasn’t bright white and healthy looking anymore but it still grew.

I got at least one decent flush out of all of them though.

I never smelled anything until like a week after I spawned them and then a few had this weird spicy fruit kind of smell going on.


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OfflineDNA_Trip
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Re: Injecting sterile water? [Re: A.k.a]
    #26437113 - 01/16/20 04:27 PM (4 years, 1 month ago)

So now that you're doing agar, you just stick to agar? Where did you get your agar from? I'm looking it up, there are so many types, and so many teks, I don't know what is for what.

Also, do you think I should try a shoebox each? Or put both into one shoebox?


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OfflineA.k.aM
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Re: Injecting sterile water? [Re: DNA_Trip]
    #26437115 - 01/16/20 04:33 PM (4 years, 1 month ago)

I’d do one each with one quart of coir each.

I got all my stuff on amazon. I had a ton of little things to buy so I signed up for the free month of amazon prime and bought everything I could think of while the shipping was free.

Agar is really cheap and easy. I just started like a month or so ago myself, my first clone tubs were spawned 8 days ago.

The best part I’m finding out is you’ll end up with all these plates full of myc from old transfers that are clean so anytime I want to grow some I can just grab a plate of that type and put it in a jar.


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OfflineDNA_Trip
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Re: Injecting sterile water? [Re: A.k.a]
    #26437127 - 01/16/20 04:40 PM (4 years, 1 month ago)

One quart of coir doesn't seem like it will give me the necessary sub height needed which is between 2-4 inches correct?

What is the type of agar you bought?


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InvisiblebodhisattaMDiscordReddit
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Re: Injecting sterile water? [Re: DNA_Trip]
    #26437152 - 01/16/20 04:58 PM (4 years, 1 month ago)

Quote:

bodhisatta said:
Maybe this will help

All these are bad. Everyone of them




All these are good





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Re: Injecting sterile water? [Re: DNA_Trip]
    #26437160 - 01/16/20 05:02 PM (4 years, 1 month ago)

Quote:

DNA_Trip said:
One quart of coir doesn't seem like it will give me the necessary sub height needed which is between 2-4 inches correct?

What is the type of agar you bought?





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I'm tired do me a favor


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Re: Injecting sterile water? [Re: cronicr]
    #26437166 - 01/16/20 05:05 PM (4 years, 1 month ago)

The necessary height is between 1 grain of spawn tall and 6 inches or more.

Quote:

RogerRabbit said:
Quote:

IAmABanana said:
Once pin heads form, stop misting the casing. Water is not the only factor in determining fruit size. Another major factor is substrate depth. What species is this anyway? (I'm assuming cubensis).

The casing doesn't really hold much moisture, it provides a micro climate for primordia growth. The water content should not be an issue on the first flush.




Sorry, but that is flat-out wrong.  I can't just let it slide.  I've worked for many years to overcome bad advice that was given out in the early days when mushrooms growing was not understood.

NEVER stop misting.  Growing mushrooms require moisture AND fresh air.  If you're providing the proper amount of fresh air, your fruits and your substrate will dry out.  This leads to small fruits.  You MUST mist from the pin stage all the way to mature fruits if you want a proper flush.

Quote:

The casing doesn't really hold much moisture



Again, incorrect.  The purpose of a casing layer(when used) is to hold moisture to support the flush.  By misting, or even pouring water directly on the casing layer surface, the mycelium that has partially colonized the casing can absorb this moisture and carry it to the substrate, where it can help the developing mushrooms to grow.

The reason deeper substrates support larger flushes and fruits is because larger substrates hold more moisture.  If you give proper moisture control to small substrates, they will perform well too.  It's all about water, as can be seen by the picture below of very large mushrooms on a very small substrate.  It's all about misting and fresh air, from initiation through harvest.
RR






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OfflineA.k.aM
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Re: Injecting sterile water? [Re: bodhisatta]
    #26437192 - 01/16/20 05:27 PM (4 years, 1 month ago)

Hey bod those bad ones you posted, the bottom left pic can you tell the ones with uncolonized areas are bad because the myc is too thick? The rhizo growth does look different also, seems longer and fatter.

All I know to look for now is the leading edge getting kind of thin and uneven but those jars don’t have that from what I can tell.

It seems like all the healthy ones are totally covered but the grain is still visible.



That rr pic with the jar is awesome, have to blow it up everytime I come across it.


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LAGM2020


Edited by A.k.a (01/16/20 05:28 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Injecting sterile water? [Re: A.k.a]
    #26437215 - 01/16/20 05:39 PM (4 years, 1 month ago)

Yes and it slowing down and not colonizing further is also a pretty good sign of a big problem


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OfflineA.k.aM
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Re: Injecting sterile water? [Re: bodhisatta]
    #26437225 - 01/16/20 05:44 PM (4 years, 1 month ago)

Ok cool thanks.

Good or bad?


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OfflineDNA_Trip
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Re: Injecting sterile water? [Re: A.k.a]
    #26437401 - 01/16/20 07:30 PM (4 years, 1 month ago)

Those would be bad then right considering my jars also and what bodhisatta just posted.


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Re: Injecting sterile water? [Re: DNA_Trip]
    #26437420 - 01/16/20 07:43 PM (4 years, 1 month ago)

I’m not sure that’s why I asked, I have a lot of trouble distinguishing between bacterial wet spots and condensation wet spots.


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