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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Injecting sterile water? [Re: DNA_Trip]
#26436091 - 01/16/20 04:44 AM (4 years, 1 month ago) |
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Seems Quote:
DNA_Trip said: I'm not sure if that was intended to be sarcastic towards me and my jars... I'm just wanting to learn, chat, and talk with other cultivators on here and learn man.
Seems like you want to tell everyone how wrong they are. If you know better than us try your jars out post pics prove us wrong. No harm in that.
No one here tried to save you the hassle and tell you to start new jars now so you get mushrooms ASAP.
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DNA_Trip
Stranger

Registered: 10/02/19
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Re: Injecting sterile water? [Re: Samskara92]
#26436147 - 01/16/20 05:45 AM (4 years, 1 month ago) |
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I’m just concerned how this could of happened because for it to happen to all three of my jars is concerning and I’m trying to understand what happened or what the source was so I know since I’m going to have to do this all over in the near future. I can’t think of anything that I did and it’s weird that all 3 of my jars are like that.
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DNA_Trip
Stranger

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Re: Injecting sterile water? [Re: bodhisatta]
#26436151 - 01/16/20 05:49 AM (4 years, 1 month ago) |
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You’re a legend on here, there’s no way I’m not listening to you Bodhisatta. I’m thinking over everything I did, and trying to pinpoint what could of happened and what the source was, etc. It’s just upsetting and concerning that how did all of my jars are like that. I would understand if it was a single one or something, but for all three...? I can’t think of what I may of done wrong, that’s what is troubling me.
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Samskara92
Certified Hot Dropper



Registered: 08/29/19
Posts: 334
Loc: Pochinki
Last seen: 8 days, 11 hours
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Re: Injecting sterile water? [Re: DNA_Trip]
#26436159 - 01/16/20 05:56 AM (4 years, 1 month ago) |
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If you injected you jars with MS syringe, than it's more likely they'll contam than not. If you want clean results, start looking into doing agar, and cleaning up cultures before inoculation.
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The Mycologist
Explorer

Registered: 05/06/16
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Re: Injecting sterile water? [Re: DNA_Trip]
#26436197 - 01/16/20 06:27 AM (4 years, 1 month ago) |
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You can pc your jars 2 hours. and post pics of your agar.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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DNA_Trip
Stranger

Registered: 10/02/19
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Last seen: 3 years, 1 month
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I didn’t do agar. I did a spore syringe. What would PC those jars for 2 hours do? For a grain transfer?
Edited by DNA_Trip (01/16/20 07:14 AM)
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trubblesome
Stranger



Registered: 11/09/19
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Re: Injecting sterile water? [Re: DNA_Trip]
#26436297 - 01/16/20 07:44 AM (4 years, 1 month ago) |
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I've had contamination that looked like this before. I don't know exactly what it is but you can see it has shriveled those grains that aren't colonizing. no matter what those granules are, the fact those berries haven't colonized with mycelium but are shriveling up means something you can't see is eating them.
PC'ing for 2 hours would kill whatever contam is growing on them so you can dump them out without unloading their nastiness in to your house. some contams can be hazardous to your health, which is why you shouldn't take a big whiff of a jar you suspect contam'd, especially if you don't know what it is.
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DNA_Trip
Stranger

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Re: Injecting sterile water? [Re: bodhisatta]
#26436338 - 01/16/20 08:27 AM (4 years, 1 month ago) |
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I’m just feeling over it concerned and upset as to what wrong or what happened...
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TheStallionMang
Do U know who yur fuckin with?


Registered: 10/18/17
Posts: 4,569
Loc:
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Re: Injecting sterile water? [Re: DNA_Trip]
#26436354 - 01/16/20 08:38 AM (4 years, 1 month ago) |
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Shit happens, try again
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trubblesome
Stranger



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Re: Injecting sterile water? [Re: DNA_Trip] 1
#26436412 - 01/16/20 09:09 AM (4 years, 1 month ago) |
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Quote:
DNA_Trip said: I’m just feeling over it concerned and upset as to what wrong or what happened...
Quote:
DNA_Trip said: I didn’t do agar. I did a spore syringe.
spore syringe to grain is what happened. learn agar, it's easy, and you'll know that you're only putting clean mycelium in to your grain jars. The time I got this contamination was from spore syringe -> LC -> grain. That was a mistake. Now i'm using agar and never looking back.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Even the best spore syringes are not axenic. Spores directly to grain is always a gamble. Sometimes you could get 95% good jars sometimes its 50/50 sometimes its 100% bad
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DNA_Trip
Stranger

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Re: Injecting sterile water? [Re: bodhisatta]
#26436779 - 01/16/20 12:46 PM (4 years, 1 month ago) |
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Would you spawn these to see what you get? Is there any harm in that? Or is that dangerous?
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A.k.a
Stranger



Registered: 10/27/19
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Re: Injecting sterile water? [Re: DNA_Trip]
#26436823 - 01/16/20 01:09 PM (4 years, 1 month ago) |
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No harm, if you have the space and supplies you might as well just for experience.
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LAGM2020     
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: Injecting sterile water? [Re: A.k.a]
#26436865 - 01/16/20 01:41 PM (4 years, 1 month ago) |
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Is this the only three jars you got or something...
I'd just do it man. So many people told me to dump these 4 or 5 jars I had. Said they aren't worth spawning. Someone even told me to throw the whole jar out so I dont spread anything (just bacteria)...
I didnt listen and spawned all of them in the same mono... I also got way more mushrooms then if I would've dumped the jars out...
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DNA_Trip
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Re: Injecting sterile water? [Re: A.k.a]
#26436971 - 01/16/20 03:09 PM (4 years, 1 month ago) |
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Yeah I'll probably do it tomorrow. I was just worried about introducing any contamination into the bulk substrate, and then some poisonous mushrooms will grow or something...? Does that sound too far fetched and I'm just being paranoid?
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DNA_Trip
Stranger

Registered: 10/02/19
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Last seen: 3 years, 1 month
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Yes, these were the only 3 jars I made from a B+ syringe. I'm only down to 2 because last night after being on the thread and getting other input from people, I popped one open to see if it passed the smell test, and it smelled like fresh mushrooms. So now I'm down to 2. I'm going to spawn to bulk tomorrow then, I'm just worried about introducing any bacteria or anything that people are saying I have in my jars into my bulk substrate and growing some bacterial poisonous mushrooms or something. Or am I just being too paranoid?
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: Injecting sterile water? [Re: DNA_Trip]
#26436983 - 01/16/20 03:13 PM (4 years, 1 month ago) |
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I'll take "things that will never happen" for $500
Lol dude ya ur paranoid for sure... if that could happen then there would be crazy news coverage over hippies dying from poisonous mushrooms...
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: Injecting sterile water? [Re: DNA_Trip]
#26436990 - 01/16/20 03:16 PM (4 years, 1 month ago) |
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Quote:
DNA_Trip said: Yes, these were the only 3 jars I made from a B+ syringe. I'm only down to 2 because last night after being on the thread and getting other input from people, I popped one open to see if it passed the smell test, and it smelled like fresh mushrooms. So now I'm down to 2. I'm going to spawn to bulk tomorrow then, I'm just worried about introducing any bacteria or anything that people are saying I have in my jars into my bulk substrate and growing some bacterial poisonous mushrooms or something. Or am I just being too paranoid?
Bacteria is fine to eat in most cases it's in your yogurt on your meat and on your skin...
If your jar was 100% colonized when u opened it it is still fine...
Most jars have some level of bacteria in them from what I've gathered...
I even grew a whole tray of bacterial mushrooms where the sub even turned yellow ish gold and had a tray that was overrun with pseudomonas bacteria... all turned out fine...
Pretty sure most bacteria dies after dehydrating them anyways..
Edited by SpunkyMonkey88 (01/16/20 03:19 PM)
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DNA_Trip
Stranger

Registered: 10/02/19
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Last seen: 3 years, 1 month
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I don't even know where to start with agar or how to do it. Did you just buy agar Petri dishes?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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So now your just gonna tell him to spawn jars at 50% colonization...good job, there you go u got the answer you wanted to hear, good luck
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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