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OfflineEclipse3130
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Registered: 10/06/13
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Is it possible to have a dirty flow hood?
    #26435604 - 01/15/20 08:13 PM (4 years, 14 days ago)

Y/N

Is it possible your success rate could be due to the actual insides of your flow hood? Especially if it was purchased used?


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"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


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OfflineDancing with Bears
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Re: Is it possible to have a dirty flow hood? [Re: Eclipse3130]
    #26435650 - 01/15/20 08:43 PM (4 years, 14 days ago)

Yes. They have a shelf life. I believe 2-5 years is the common expectancy (depending on environment, quality of filter, use frequency, and effectiveness of pre-filter)
I would buy a shitty off brand new before buying anything used. That is a bummer man. Hope you can at least salvage the box you made for it.


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OfflineEclipse3130
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Registered: 10/06/13
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Re: Is it possible to have a dirty flow hood? [Re: Dancing with Bears]
    #26435656 - 01/15/20 08:48 PM (4 years, 14 days ago)

I purchased one used which is probably the first mistake. Envirco hood.

But theres still a chance it is my human error, as I'm new to using hoods. I'm hoping and betting on that

I will do a few more serious precise and clean transfers from clean material and see if they fail to bacteria again. If they keep failing I'll blame it on the hood and go back to a box for the time being.


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"


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OfflineGrowtech
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Registered: 11/09/19
Posts: 165
Last seen: 2 years, 5 months
Re: Is it possible to have a dirty flow hood? [Re: Eclipse3130]
    #26435794 - 01/15/20 10:22 PM (4 years, 14 days ago)

One way to test it would be to leave Petri dishes (with agar/media of course) in front of it for awhile.  Like just let it sit there for a few hours with it on, then close up dishes and see if anything grows.  At least then you take your aseptic work (mostly) out of the equation.


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