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OfflineDNA_Trip
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Injecting sterile water?
    #26434585 - 01/15/20 09:39 AM (4 years, 1 month ago)

I believe my grains may be too dry, and that’s why they’re colonizing very slowly even after my shake and haven’t reached full colonization yet. Someone on here told me to try injecting 5 cc’s of sterile water and shaking again. Has anyone tried this and how were the results and your experiences? Or should I forget about the sterile water? Will they still eventually reach full colonization no matter what?


Edited by DNA_Trip (01/15/20 09:40 AM)


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OfflineBLINKfan420
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Re: Injecting sterile water? [Re: DNA_Trip]
    #26434592 - 01/15/20 09:44 AM (4 years, 1 month ago)

Your grains need to be hydrated properly from the get go.  Hence the bringing them to boil for 10 - 15 minutes and then letting them dry out for a bit until they pass the toilet paper test. 

I'm not sure but I wouldn't think that injecting some sterile water into the jars would help in the same way that having the grains properly hydrated would. :shrug:

Let's see what some others say first.

BLiNK


--------------------



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OfflineThe Mycologist
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Re: Injecting sterile water? [Re: DNA_Trip]
    #26434593 - 01/15/20 09:46 AM (4 years, 1 month ago)

I wouldnt do that. Its about internal grain moisture. If you followed a tek they are probably fine. You dont want to see visible moisture in your jars.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
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InvisibleTheStallionMang
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Re: Injecting sterile water? [Re: DNA_Trip]
    #26434596 - 01/15/20 09:46 AM (4 years, 1 month ago)

We need pics or at least more info to evaluate. From what I've seen, nobody makes their grains too dry and I've never heard of injecting water in so that sounds risky to me.

They will not reach full colonization no matter what though.  Could be contams are stopping things but need pics or more info


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OfflineDNA_Trip
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Re: Injecting sterile water? [Re: TheStallionMang]
    #26434710 - 01/15/20 11:20 AM (4 years, 1 month ago)

They will reach full colonization at some point? Or will never?


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OfflineDNA_Trip
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Re: Injecting sterile water? [Re: BLINKfan420]
    #26434715 - 01/15/20 11:24 AM (4 years, 1 month ago)

I soaked my grains for 24 hours, and then boiled for 10 minutes and let the steam evaporate. It’s not my first rodeo. I just haven’t seen colonization this slow, but maybe I just need to be patient. I shook my jars one week ago, and it’s been one week since and they are still not fully colonized. Someone said my grains look to dry, and to inject some sterile water and reshake to redistribute moisture. Maybe nothing is wrong, who knows, and this is just taking a lot longer to colonize, but it’s been 2 weeks so far since I inoculated and one week since I shook.


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OfflineDNA_Trip
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Re: Injecting sterile water? [Re: BLINKfan420]
    #26434720 - 01/15/20 11:28 AM (4 years, 1 month ago)

I did everything you said as far as prepping my grain. I just don’t understand the slow colonization after one week since I shook it. I’m used to them already fully colonizing about 5 days after my shake. It’s been one week since I shook, two weeks since inoculation, and it’s still only 75% colonized. I keep thinking it might be stalling out on me, but when compared to a couple days ago it seems to have colonized a bit more. It’s just going so slow. Usually after I shake it’s only several days and they should be fully colonized. Do you think another shake would help?


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Offlineattednev
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Re: Injecting sterile water? [Re: DNA_Trip]
    #26434751 - 01/15/20 11:48 AM (4 years, 1 month ago)

Dont add anything. I've had jars that looked waaay too dry still colonize. It just took longer.


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Invisiblewildernessjunkie
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Re: Injecting sterile water? [Re: attednev]
    #26434953 - 01/15/20 01:27 PM (4 years, 1 month ago)

Dont inject anything else. Poor recovery after a shake is a good indicator that there may be some bacteria in there. Posting pics always helps.


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OfflinecronicrFacebook
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Re: Injecting sterile water? [Re: wildernessjunkie]
    #26434983 - 01/15/20 01:42 PM (4 years, 1 month ago)

Quote:

DNA_Trip said:
Here you, a picture of each of my 3 jars.





Quote:

cronicr said:

They look contaminated to me:shrug:




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Invisiblewildernessjunkie
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Re: Injecting sterile water? [Re: cronicr]
    #26434993 - 01/15/20 01:50 PM (4 years, 1 month ago)

Quote:

cronicr said:
Quote:

DNA_Trip said:
Here you, a picture of each of my 3 jars.





Quote:

cronicr said:

They look contaminated to me:shrug:







Nice catch Cron...

Also, sterile water isn't gonna fix a problem that looks like yeast to me.


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OfflineDNA_Trip
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Re: Injecting sterile water? [Re: wildernessjunkie]
    #26435081 - 01/15/20 02:27 PM (4 years, 1 month ago)

Where is the yeast you guys keep saying??? Those are gypsum granules, not yeast. Yeast would have white granules on the grains themselves and also smell. I didn’t say there was poor recovery, it bounced back just fine, I was just expecting it to be fully colonized by now. I’m still well within the time frame also of colonization. Some just take longer than others based on different variables correct? I mean what yeast???


Edited by DNA_Trip (01/15/20 02:31 PM)


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OfflineDNA_Trip
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Re: Injecting sterile water? [Re: cronicr]
    #26435124 - 01/15/20 02:56 PM (4 years, 1 month ago)

If I popped one open and took a whiff shouldn't it smell then if it's yeast?


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InvisibleTheStallionMang
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Re: Injecting sterile water? [Re: DNA_Trip]
    #26435218 - 01/15/20 04:02 PM (4 years, 1 month ago)

Have you been leaving the foil on while it colonizes?  Usually the foil is taken off and the micro-pore tape or poly-fill allows for gas exchange.  Maybe they suffocated?


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OfflinecronicrFacebook
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Re: Injecting sterile water? [Re: TheStallionMang]
    #26435221 - 01/15/20 04:03 PM (4 years, 1 month ago)

Foil won't suffocate jars


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OfflineDNA_Trip
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Re: Injecting sterile water? [Re: TheStallionMang]
    #26435237 - 01/15/20 04:09 PM (4 years, 1 month ago)

Yeah foil doesn't do that, and has never been a problem for me. I think I'm going to try shaking again and see what happens. I'm debating whether I should pop one open and take a whiff....? If it's yeast (I don't see any signs people are saying) then it should smell bad correct?


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OfflineDNA_Trip
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Re: Injecting sterile water? [Re: cronicr]
    #26435247 - 01/15/20 04:11 PM (4 years, 1 month ago)

Correct, and that's never been a problem for me. Do you think I should re shake again? I'm also debating whether I should pop one open and give it a whiff...? If it's yeast like you guys think (I don't see how it can be, that is gypsum, not yeast granules) then it should smell bad correct?


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InvisibleTheStallionMang
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Re: Injecting sterile water? [Re: DNA_Trip]
    #26435248 - 01/15/20 04:13 PM (4 years, 1 month ago)

Sometimes you can smell it thru the poly-fill or tape. Try that before popping it open


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OfflineDNA_Trip
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Re: Injecting sterile water? [Re: TheStallionMang]
    #26435279 - 01/15/20 04:23 PM (4 years, 1 month ago)

There are no smells coming through any of the poly. Would you be able to smell it through the poly for sure? I thought it needs to be opened? Or would you definitely smell a contamination through the poly?


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OfflineDNA_Trip
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Re: Injecting sterile water? [Re: cronicr]
    #26435281 - 01/15/20 04:26 PM (4 years, 1 month ago)

I've never shaken a jar more than once, but do you think a re shake could help with anything? Maybe it will colonize the rest I need?


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