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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Returning Cultivator’s questions on agar.
#26430424 - 01/12/20 10:21 PM (4 years, 1 month ago) |
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I have just return from taking a decent break from cultivating and was attempting to pick up where I left off. Last time I allowed 3 bags of potatoes to rot in my kitchen cause all my agar to completely get screwed. That aside, I remember struggling with agar attempting to do about 10-15 plates at once as I was trying to keep 6 monos going. Is there a good place to start with agar cloning, syringes, or spore prints from a fresh grow? I guess before I start another mono should I begin with agar or inject straight into jars and get a spore print? I remember spore work much better than the syringes any advice would help.
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Growtech
Stranger

Registered: 11/09/19
Posts: 165
Last seen: 2 years, 5 months
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Re: Returning Cultivator’s questions on agar. [Re: PiptheGreAtest]
#26430480 - 01/12/20 11:19 PM (4 years, 1 month ago) |
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I'd start with agar. If you have ms syringe, put a drop of that on agar and spread it around (with inoculation loop or whatever works for you). Syringes are most often than not bacterial... do a couple transfers to get a non-contaminated plate. Then go agar to grain.
Too much work to try and do major isolation work from a syringe unless you are just wanting to nerd out and do it for fun.
But you'll be much happier starting your grain (or whatever you want to use) with something contamination-free. Then do whatever you want... clone, get prints, etc.
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Re: Returning Cultivator’s questions on agar. [Re: Growtech]
#26430706 - 01/13/20 05:31 AM (4 years, 1 month ago) |
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That seems like a good place to start. Can you direct me to a good agar tek forum. I remember doing agar but had a huge contamination problem but could of been the potatoes. I remember 1 master plate of agar can creat like 10 more agar plates and A2G transfer tiny pieces from plate to grain so one plate easily do 5-7 jars. Correct line of thinking?
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tramalot
Stranger


Registered: 12/13/19
Posts: 199
Loc: USA
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Re: Returning Cultivator’s questions on agar. [Re: PiptheGreAtest]
#26430727 - 01/13/20 06:16 AM (4 years, 1 month ago) |
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Violet ? seems like a good place for some of the newest "tek's"
-------------------- It's the Journey I enjoy!
Edited by tramalot (01/13/20 06:17 AM)
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Kimble
Idiot

Registered: 03/08/18
Posts: 508
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Re: Returning Cultivator’s questions on agar. [Re: tramalot]
#26430731 - 01/13/20 06:27 AM (4 years, 1 month ago) |
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Bigger wedge of agar makes the jar colonize faster too bro.
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Re: Returning Cultivator’s questions on agar. [Re: Kimble]
#26430869 - 01/13/20 08:57 AM (4 years, 1 month ago) |
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Not to keen on Violet, trying to do larger quantity(kg) so monotub would likely be the best way although keeping grain jars colonized from what I remember is easy from afar plates. Is it ok to store colonized agar in fridge?
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Growtech
Stranger

Registered: 11/09/19
Posts: 165
Last seen: 2 years, 5 months
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Re: Returning Cultivator’s questions on agar. [Re: PiptheGreAtest]
#26430998 - 01/13/20 10:19 AM (4 years, 1 month ago) |
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Quote:
PiptheGreAtest said: Not to keen on Violet, trying to do larger quantity(kg) so monotub would likely be the best way although keeping grain jars colonized from what I remember is easy from afar plates. Is it ok to store colonized agar in fridge?
Yes, you can keep colonized agar in the fridge to stall growth.
This is probably a good place to start for you: https://www.shroomery.org/forums/showflat.php/Number/21922023
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Re: Returning Cultivator’s questions on agar. [Re: Growtech]
#26433437 - 01/14/20 03:16 PM (4 years, 1 month ago) |
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Was refreshing on that tek last night, let me run something by the experience audience here. I’m regaining my train of thought of the processes I was using and want to know if this is the most effective way to create high yielding grows in the shortest period of time. I began my process with isolating a few containment free agar dishes which I transferred into blender less liquid inoculate, here first question how many ml of sterile water can 1 petting fish be effective with and how many quarts can you inoculate with that dish? From what I remember it’s like 25-50ml per quart which brings me to my next question. I was using large spawn bags with 6qt in each and inoculating them with the liquid is it one Petri dish to a bag so 300ml? Than I was using the bucket tek to prepare the bulk substrate, here I was struggling is it necessary to sanitize substrate, from what I understand the spawn should be strong enough that it doesn’t contaminate or so you don’t kill the nutrients within the substrate? I was using a 50/50 Verm coir substrate and seen decent yields is there a better yielding substrate not as prone to contamination? I really appreciate the help getting these process all straightened out if anyone has advice how to speed the process up or increase yields that would be great.
Edited by PiptheGreAtest (01/14/20 03:18 PM)
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