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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: SpunkyMonkey88]
#26433363 - 01/14/20 02:43 PM (4 years, 1 month ago) |
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It's not your pc cycle . Molds are killed off even with pastirinzing temps so unless you just sat them in hot tap water that's not your issue. Your issue is inoculant , if it was your filters you would see it start at the top of your grain and would likely have more than one type of contaminate , a shitty technique you may lose half your jars but get lucky with the other half, the common factor here is your inoculant
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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smokedtrolli
Prologue



Registered: 04/01/19
Posts: 224
Last seen: 1 day, 20 hours
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: gizmo1]
#26433413 - 01/14/20 03:06 PM (4 years, 1 month ago) |
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Quote:
gizmo1 said:
Can't say with 100% certainty but it could be. Not venting combined with low sterilization times could very well do it.
Can we reiterate the discussion of sterility during inoculation? My understanding was that that is extremely important yet a few of you are saying you can get away with open-air inoculation? I'm confused.
-------------------- Money is too valuable to be moral.
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smokedtrolli
Prologue



Registered: 04/01/19
Posts: 224
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: cronicr]
#26433416 - 01/14/20 03:09 PM (4 years, 1 month ago) |
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Quote:
cronicr said: It's not your pc cycle . Molds are killed off even with pastirinzing temps so unless you just sat them in hot tap water that's not your issue. Your issue is inoculant , if it was your filters you would see it start at the top of your grain and would likely have more than one type of contaminate , a shitty technique you may lose half your jars but get lucky with the other half, the common factor here is your inoculant
So basically there was mold on that plate that I didn't notice. I'm willing to believe that I guess; perhaps it was premature or something and hadn't yet released spores?
-------------------- Money is too valuable to be moral.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
#26433427 - 01/14/20 03:12 PM (4 years, 1 month ago) |
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If you're using needles and injection ports you can do inoculation open air
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
#26433428 - 01/14/20 03:13 PM (4 years, 1 month ago) |
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Yeah there are molds that don't tend to sporulate till they get fresh air, more nutrition, broken colony ECT, best bet is to start fresh with new filters and post pics of your cultures before going to grain
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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smokedtrolli
Prologue



Registered: 04/01/19
Posts: 224
Last seen: 1 day, 20 hours
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: cronicr]
#26433433 - 01/14/20 03:15 PM (4 years, 1 month ago) |
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Quote:
cronicr said: Yeah there are molds that don't tend to sporulate till they get fresh air, more nutrition, broken colony ECT, best bet is to start fresh with new filters and post pics of your cultures before going to grain
I have two other plates that are colonized but the lid is covered with condensation from the inside; supplying a clear picture would be difficult. Use or discard?
-------------------- Money is too valuable to be moral.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
#26433436 - 01/14/20 03:16 PM (4 years, 1 month ago) |
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Make transfers and take pics of the donor plate afterward
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 1 day, 8 hours
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: cronicr]
#26433441 - 01/14/20 03:19 PM (4 years, 1 month ago) |
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You can put something warm on the plate to clear up condensation real quick too if you want. Sounds like a lot of people use a cup of hot water
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LAGM2020     
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smokedtrolli
Prologue



Registered: 04/01/19
Posts: 224
Last seen: 1 day, 20 hours
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: cronicr]
#26433442 - 01/14/20 03:19 PM (4 years, 1 month ago) |
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Quote:
cronicr said: Make transfers and take pics of the donor plate afterward
Gotcha. Unrelated: what's the max amount of time sterilized grain should be kept without being inoculated? Just prepped a bunch of grain that I'd rather not waste.
-------------------- Money is too valuable to be moral.
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
#26436495 - 01/16/20 10:04 AM (4 years, 1 month ago) |
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Quote:
smokedtrolli said:
Quote:
cronicr said: Make transfers and take pics of the donor plate afterward
Gotcha. Unrelated: what's the max amount of time sterilized grain should be kept without being inoculated? Just prepped a bunch of grain that I'd rather not waste.
Of its properly sterilized, many months .. I would think until it dries out or the structure of the grains start to degrade...
I've used jars that were 3 months old with no problems whatsoever
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