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rickyswamps
Bad Apple



Registered: 11/08/18
Posts: 1,192
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
#26431702 - 01/13/20 04:09 PM (4 years, 1 month ago) |
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What kind of lids? pics?
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
#26431704 - 01/13/20 04:12 PM (4 years, 1 month ago) |
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Quote:
smokedtrolli said: I shook most of them after not seeing any mold and having some mycelium growth off of the wedge. Of the ones I did not shake, the contamination actually did originate from the top.
If you didn't see any growth (mushroom or otherwise) from anywhere but the top then you could probably forget the PC theory I suggested and assume it was from the lids or the agar or sloppy innlocation technique...
All u can do at this point is take some transfers from that slice u have left and tighten up your technique when you make your second attempt...
I think this is super weird... I've never seen 100% contamination rate before except for dumb shit like open air inoculated grain jars or something crazy like that..
I'm also very new to this as well so....
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StygianKnight
A Mushroom

Registered: 03/12/12
Posts: 2,717
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: SpunkyMonkey88]
#26431762 - 01/13/20 04:56 PM (4 years, 1 month ago) |
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How old are those jars and how long after the shake? Thatโs an impressive amount of penicillium!
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smokedtrolli
Prologue



Registered: 04/01/19
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: StygianKnight]
#26432951 - 01/14/20 10:37 AM (4 years, 1 month ago) |
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Quote:
StygianKnight said: How old are those jars and how long after the shake? Thatโs an impressive amount of penicillium!
2-3 days after the shake, the jars are probably a week old. Pretty aggressive stuff.
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smokedtrolli
Prologue



Registered: 04/01/19
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: rickyswamps]
#26432954 - 01/14/20 10:39 AM (4 years, 1 month ago) |
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Quote:
rickyswamps said: What kind of lids? pics?
1/4inch holes stuffed with polyfill. I'm not sure if that necessitates a picture or not considering half of the people who have grown w/ grains do the same thing. Nothing really special.
-------------------- Money is too valuable to be moral.
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
#26432958 - 01/14/20 10:41 AM (4 years, 1 month ago) |
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Quote:
smokedtrolli said:
Quote:
rickyswamps said: What kind of lids? pics?
1/4inch holes stuffed with polyfill. I'm not sure if that necessitates a picture or not considering half of the people who have grown w/ grains do the same thing. Nothing really special.
Ya that's pretty hard to fuk up 9/9times... weird dude.
I cant process how this is possible unless you licked your scalpel between jars or something
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smokedtrolli
Prologue



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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: SpunkyMonkey88]
#26432960 - 01/14/20 10:44 AM (4 years, 1 month ago) |
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Quote:
SpunkyMonkey88 said:
Quote:
smokedtrolli said:
Quote:
rickyswamps said: What kind of lids? pics?
1/4inch holes stuffed with polyfill. I'm not sure if that necessitates a picture or not considering half of the people who have grown w/ grains do the same thing. Nothing really special.
Ya that's pretty hard to fuk up 9/9times... weird dude.
I cant process how this is possible unless you licked your scalpel between jars or something
I think it's my SAB actually. The bottom lacks uniformity so it isn't flush with the table, leaving space for air to enter under the SAB. That's my only guess. The colonized agar plate was left long enough for anything that wasn't mycelium to develop and reveal itself.
Probably gonna go buy a new container and make another SAB. And just be more rigorous with cleaning the work area before hand too.
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SpunkyMonkey88
Stranger



Registered: 10/08/19
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
#26432970 - 01/14/20 10:53 AM (4 years, 1 month ago) |
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Ya dude that sounds like a good plan. My table isn't uniformly flat either. It's made of 3 1' wide boards with a 1/4" gap in between them. So I lay down a big piece of plastic then a layer of paper toweling then i spray it down...
I've worked with just under 100 agar plates always pouring more than I need so they always sit for a week before using and I've only ever seen 1 plate contaminate before using it...
Just how I do shit and it works well for me...
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A.k.a
Stranger



Registered: 10/27/19
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
#26432975 - 01/14/20 10:56 AM (4 years, 1 month ago) |
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I have no real knowledge about this but it seems likely it has something to do with the grains. Maybe the jars werenโt sterile?
If the agar was good that and the grains are really the only common denominators. I canโt imagine it being the SAB, it seems like you could do it open air and not get that much/consistent contamination.
Definitely weird.
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LAGM2020     
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: A.k.a]
#26432984 - 01/14/20 11:01 AM (4 years, 1 month ago) |
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Quote:
A.k.a said: I have no real knowledge about this but it seems likely it has something to do with the grains. Maybe the jars werenโt sterile?
If the agar was good that and the grains are really the only common denominators. I canโt imagine it being the SAB, it seems like you could do it open air and not get that much/consistent contamination.
Definitely weird.
That's what I was saying at first but dude seems pretty confident he didnt fuk up the PC cycle...
He said he ran it for 1.5 hrs I always go for 2 so I dont know if that 30 mins could of left that contaminate in every jar or not...
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: SpunkyMonkey88]
#26433017 - 01/14/20 11:45 AM (4 years, 1 month ago) |
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Quote:
SpunkyMonkey88 said:
Quote:
smokedtrolli said:
Quote:
rickyswamps said: What kind of lids? pics?
1/4inch holes stuffed with polyfill. I'm not sure if that necessitates a picture or not considering half of the people who have grown w/ grains do the same thing. Nothing really special.
Ya that's pretty hard to fuk up 9/9times... weird dude.
I cant process how this is possible unless you licked your scalpel between jars or something
Even licking the scalpel couldn't cause this. More likely that would cause a pretty bad case of bacteria.
OP 1.5 hours ain't bad but for oats I'd definitely increase to 2 hours and that also depends on your elevation. Also I didn't see anyone ask did you vent your PC before building pressure? This is important. I'd do another run. Stuff polyfil real tight, vent pc, and sterilize for 2 hours this time. Use the same culture but transfer to a new plate and post pics here so we can try to help you identify if anything is hiding in there.
With any luck you will have clean spawn in no time.
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smokedtrolli
Prologue



Registered: 04/01/19
Posts: 224
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: gizmo1]
#26433095 - 01/14/20 12:53 PM (4 years, 1 month ago) |
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Quote:
gizmo1 said: Also I didn't see anyone ask did you vent your PC before building pressure? This is important.
Considering I don't know what you mean by "vent", no, lol. Oops? I usually just everthing clean w/ bleach and soap before starting over again.
-------------------- Money is too valuable to be moral.
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
#26433109 - 01/14/20 01:01 PM (4 years, 1 month ago) |
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He means putting the jars in the PC and the lid on the PC but leaving the "jiggler/rocker" off while the steam vents out for 10 mins or so... they say that it is pretty important .. I always do for that reason but I've also forgot and used to not vent until reading that with out any notable contaminations...
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: SpunkyMonkey88]
#26433127 - 01/14/20 01:09 PM (4 years, 1 month ago) |
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Quote:
SpunkyMonkey88 said: He means putting the jars in the PC and the lid on the PC but leaving the "jiggler/rocker" off while the steam vents out for 10 mins or so... they say that it is pretty important .. I always do for that reason but I've also forgot and used to not vent until reading that with out any notable contaminations...
This just means you weren't pcing as long as you thought you were well not at temp anyways. If using a presto steam still escapes so you will eventually get the temps up but if you are only pcing for a hour and a half without venting then who knows how long you were actually at sterilization temps. You want only steam in the pc not steam and air pockets. The temperature of the air pockets will be much lower than the temperature of the steam. Always vent your PC.
-------------------- Trade List ๐๐๐ 6 hole Mini Monos
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smokedtrolli
Prologue



Registered: 04/01/19
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: gizmo1]
#26433130 - 01/14/20 01:11 PM (4 years, 1 month ago) |
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Quote:
gizmo1 said:
Quote:
SpunkyMonkey88 said: He means putting the jars in the PC and the lid on the PC but leaving the "jiggler/rocker" off while the steam vents out for 10 mins or so... they say that it is pretty important .. I always do for that reason but I've also forgot and used to not vent until reading that with out any notable contaminations...
This just means you weren't pcing as long as you thought you were well not at temp anyways. If using a presto steam still escapes so you will eventually get the temps up but if you are only pcing for a hour and a half without venting then who knows how long you were actually at sterilization temps. You want only steam in the pc not steam and air pockets. The temperature of the air pockets will be much lower than the temperature of the steam. Always vent your PC.
Do you think that was the source of my penicillium tragedy?
-------------------- Money is too valuable to be moral.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
#26433144 - 01/14/20 01:16 PM (4 years, 1 month ago) |
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Quote:
smokedtrolli said:
Quote:
gizmo1 said:
Quote:
SpunkyMonkey88 said: He means putting the jars in the PC and the lid on the PC but leaving the "jiggler/rocker" off while the steam vents out for 10 mins or so... they say that it is pretty important .. I always do for that reason but I've also forgot and used to not vent until reading that with out any notable contaminations...
This just means you weren't pcing as long as you thought you were well not at temp anyways. If using a presto steam still escapes so you will eventually get the temps up but if you are only pcing for a hour and a half without venting then who knows how long you were actually at sterilization temps. You want only steam in the pc not steam and air pockets. The temperature of the air pockets will be much lower than the temperature of the steam. Always vent your PC.
Do you think that was the source of my penicillium tragedy?
Can't say with 100% certainty but it could be. Not venting combined with low sterilization times could very well do it.
-------------------- Trade List ๐๐๐ 6 hole Mini Monos
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A.k.a
Stranger



Registered: 10/27/19
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: gizmo1]
#26433173 - 01/14/20 01:24 PM (4 years, 1 month ago) |
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Sounds likely.
Venting I always thought you let it run until a solid cone of steam was coming out.
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LAGM2020     
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: A.k.a]
#26433237 - 01/14/20 01:53 PM (4 years, 1 month ago) |
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So a pressure cooker that wasn't vented but still reads X-psi is actually cooler than the same PC vented at the same PSI?
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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,234
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: SpunkyMonkey88]
#26433251 - 01/14/20 01:57 PM (4 years, 1 month ago) |
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Quote:
SpunkyMonkey88 said: So a pressure cooker that wasn't vented but still reads X-psi is actually cooler than the same PC vented at the same PSI?
A write up from Bod
The Importance of Venting a Pressure Cooker or Autoclave
BOD's Easy AF how to use your pressure cooker for mycology TEK
-------------------- All I need are some tasty waves, a cool buzz, and Iโm fine. Whatever you decide wonโt really impact our survival Close your eyes, and do the best that you can
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: Camera93]
#26433322 - 01/14/20 02:24 PM (4 years, 1 month ago) |
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Quote:
Camera93 said:
Quote:
SpunkyMonkey88 said: So a pressure cooker that wasn't vented but still reads X-psi is actually cooler than the same PC vented at the same PSI?
A write up from Bod
The Importance of Venting a Pressure Cooker or Autoclave
BOD's Easy AF how to use your pressure cooker for mycology TEK
Seems like an interesting read I'm gonna check it out when I'm off work...
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