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OfflineJarhead3521S
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Registered: 09/26/19
Posts: 515
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Re: Let's All Grow Mushrooms 2020 [Re: trubblesome]
    #26432002 - 01/13/20 07:18 PM (4 years, 1 month ago)

1/13/2020: FH construction has commenced!  Unfortunately my lazy ass left plywood out leaned up on the back porch and things got a little warped, so we’re flattening things back out.  Snatched (3)20x25x2 hepa from work, stacking them in.  Have a 1/3hp 115v blower from a furnace to power it.  I plan on getting a speed controller and will use my anomometer to dial it in.  Still have the cabinet to cover the blower to build too.....SAB has been good to me, but I made my arm holes too far apart and it’s killing my shoulders.  T1s are also looking good, should know by end of week or sooner if I need to make another transfer or send it to the grains.



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InvisibleYogiBear
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Registered: 08/24/19
Posts: 845
Re: Let's All Grow Mushrooms 2020 [Re: Durgin]
    #26432035 - 01/13/20 07:45 PM (4 years, 1 month ago)

Quote:

Durgin said:
Quote:

YogiBear said:
Hey there Durgin. I do no pours with PP5 petris (in my signature) and do a quick wipe on my lids when they cool in front of my flow hood with no contamination. Maybe this could work for you?





That could work with a hood, but definitely not a SAB. The water in these is also between the edges though, so it's more work and contamination risk, definitely not ideal. I'm just going to go back to regular pouring - I'm pretty solid at that (maybe 1-2% contam rate in my SAB) so not a big deal.

May need some of those pp5 plates though when they're back in stock. I've quickly figured out why more people don't use glass, but at the same time I do prefer reusable supplies - I don't have to feel guilty about taking extra transfers when it's just a little extra agar.




I totally get that having the SAB and all. If I get to much more crazy I will be doing regular pours. Just tapped out on my myc budget right now and using what I have in my artillery. I'm only doing 2 transfer per plate due to only having 24 PP5s to work with total.


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Offlinefootpath
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Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 3 months
Re: Let's All Grow Mushrooms 2020 [Re: Jarhead3521]
    #26432036 - 01/13/20 07:46 PM (4 years, 1 month ago)

Pushin' on.

1 • 13 • 20

•  T1 Day 7  •




Gonna transfer out from all but one that I'll attempt on grains. (And transfer that one at that point, too.)
I have a sneaking suspicion that I accidentally made my last batch of agar way too nutritious - hence the exclusively tomentose growth.
I transferred some of my other cultures that perform almost exclusively rhizomorphic to this recent batch of plates only to find them fuzzin' out...


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InvisibleYogiBear
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Registered: 08/24/19
Posts: 845
Re: Let's All Grow Mushrooms 2020 [Re: Jarhead3521]
    #26432037 - 01/13/20 07:47 PM (4 years, 1 month ago)

Quote:

Jarhead3521 said:
1/13/2020: FH construction has commenced!  Unfortunately my lazy ass left plywood out leaned up on the back porch and things got a little warped, so we’re flattening things back out.  Snatched (3)20x25x2 hepa from work, stacking them in.  Have a 1/3hp 115v blower from a furnace to power it.  I plan on getting a speed controller and will use my anomometer to dial it in.  Still have the cabinet to cover the blower to build too.....SAB has been good to me, but I made my arm holes too far apart and it’s killing my shoulders.  T1s are also looking good, should know by end of week or sooner if I need to make another transfer or send it to the grains.







One of my sons is a welder. Other is a graphic artist. Twins, go figure.


Edited by YogiBear (01/13/20 07:58 PM)


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InvisibleYogiBear
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Registered: 08/24/19
Posts: 845
Re: Let's All Grow Mushrooms 2020 [Re: footpath]
    #26432066 - 01/13/20 08:01 PM (4 years, 1 month ago)

Quote:

footpath said:
Pushin' on.

1 • 13 • 20

•  T1 Day 7  •




Gonna transfer out from all but one that I'll attempt on grains. (And transfer that one at that point, too.)
I have a sneaking suspicion that I accidentally made my last batch of agar way too nutritious - hence the exclusively tomentose growth.
I transferred some of my other cultures that perform almost exclusively rhizomorphic to this recent batch of plates only to find them fuzzin' out...






What kind of light setup do you have going there bro? With the whole split light thing going?


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OfflineJarhead3521S
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Registered: 09/26/19
Posts: 515
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Last seen: 13 days, 21 hours
Re: Let's All Grow Mushrooms 2020 [Re: YogiBear]
    #26432079 - 01/13/20 08:07 PM (4 years, 1 month ago)

Quote:

YogiBear said:
Quote:

Jarhead3521 said:
1/13/2020: FH construction has commenced!  Unfortunately my lazy ass left plywood out leaned up on the back porch and things got a little warped, so we’re flattening things back out.  Snatched (3)20x25x2 hepa from work, stacking them in.  Have a 1/3hp 115v blower from a furnace to power it.  I plan on getting a speed controller and will use my anomometer to dial it in.  Still have the cabinet to cover the blower to build too.....SAB has been good to me, but I made my arm holes too far apart and it’s killing my shoulders.  T1s are also looking good, should know by end of week or sooner if I need to make another transfer or send it to the grains.







One of my sons is a welder. Other is a graphic artist. Twins, go figure.




I’m hvac service tech by trade, but I like to burn rods and weld shit/fix old implements that I have cheap or free.....turned a straight blade for a tractor into a nice wide muck blade for mini excavator bucket, that worked out amazingly when we were clearing our property and ran into the rainy season.


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Offlinefootpath
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Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 3 months
Re: Let's All Grow Mushrooms 2020 [Re: YogiBear]
    #26432080 - 01/13/20 08:08 PM (4 years, 1 month ago)

Just spliced two photos with opposing light sources.

Mounted plate + mounted camera = seamless splicing.


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Offlinespiritlands
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Registered: 06/21/10
Posts: 1,616
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Re: Let's All Grow Mushrooms 2020 [Re: A.k.a]
    #26432170 - 01/13/20 08:54 PM (4 years, 1 month ago)

Quote:

A.k.a said:
Is there any reason not to take a transfer from a spot that just germinated? I have a bunch of spots that are by themselves that are still barely visible.



I don't like to and won't bc I want to take a transfer away from anything that came off the print. I'll prob take my t1 tonight. I'm slow playing my pitris till I see something I like.


--------------------


Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:




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InvisibleBuddaking
Genetics
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Registered: 12/18/09
Posts: 1,775
Loc: No Mans Land
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Re: Let's All Grow Mushrooms 2020 [Re: spiritlands] * 1
    #26432188 - 01/13/20 09:00 PM (4 years, 1 month ago)

:whathesaid:

Only time i transfer right off is if i am on a rescue mission. Let em go a little longer & make fewer more precise transfers before going to grain


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  AMU Q & A


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OfflineInthepit
Aum Mani Padme Hum
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Registered: 08/20/19
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Re: Let's All Grow Mushrooms 2020 [Re: Durgin]
    #26432364 - 01/13/20 11:09 PM (4 years, 1 month ago)

Quote:

Durgin said:
I've quickly figured out why more people don't use glass,



Ok, I hate to nit pick, but it's going to drive me crazy since I was really Jonesing for the whole glass petri, innoc loop, lab coat thang!

:specialralph:

What did you figure out about glass petris?

Call me a nerd (wanna be), I even had a journal for my aquarium, but I just love this whole equipment, research aspect.

This grow along, CBK!, has been fantastic at flushing out details, things the pros didn't even realize were important for us laymen.
:popcorn:


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


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Offlinen0bel
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Re: Let's All Grow Mushrooms 2020 [Re: Inthepit]
    #26432391 - 01/13/20 11:29 PM (4 years, 1 month ago)

Merrrrrrr sub too dry


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InvisibleCaps McGee
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Registered: 10/28/17
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Re: Let's All Grow Mushrooms 2020 [Re: n0bel]
    #26432409 - 01/13/20 11:46 PM (4 years, 1 month ago)

Looks on to me :shrug:


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Offlinen0bel
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Re: Let's All Grow Mushrooms 2020 [Re: Caps McGee]
    #26432458 - 01/14/20 01:19 AM (4 years, 1 month ago)

What about that weird crinkle cap tho?


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Offlineroarkell
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Registered: 08/29/19
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Re: Let's All Grow Mushrooms 2020 [Re: n0bel]
    #26432467 - 01/14/20 01:38 AM (4 years, 1 month ago)

I usually get one or two weirdos outta every box... not weird enough to call a mutant but still oddball enough... lol


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OfflineRandalf the Grey
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Re: Let's All Grow Mushrooms 2020 [Re: Inthepit]
    #26432515 - 01/14/20 02:55 AM (4 years, 1 month ago)

Quote:

Inthepit said:
Quote:

Durgin said:
I've quickly figured out why more people don't use glass,



Ok, I hate to nit pick, but it's going to drive me crazy since I was really Jonesing for the whole glass petri, innoc loop, lab coat thang!

:specialralph:

What did you figure out about glass petris?

Call me a nerd (wanna be), I even had a journal for my aquarium, but I just love this whole equipment, research aspect.

This grow along, CBK!, has been fantastic at flushing out details, things the pros didn't even realize were important for us laymen.
:popcorn:



I don't use glass petris but I totally get where youre coming from. I tried to build a collapsible "clean room" early on before I realized I don't know what I'm doing. Now I have my SAB and mag stirrer\hot plate and such set up in my "lab" which is an isolated room used only for "lab" work. I have a white coat that I use only for this work that stays in the lab. I love using my cell treat petris and the whole set up because, honestly, it means I get to play scientist like a kid again.
Just saying, you dont need glass to play scientist...


--------------------
Randalf the Grey
Lost in the right direction
A wizard is never late..........but I can try
Randalf's Book of Genesis: In The Beginning - Teks, ratios, recipes and log entries
LAGM2O2O Grow Log
Genesis II: Electric Boogaloo - The Restart


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OfflineInthepit
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Re: Let's All Grow Mushrooms 2020 [Re: Randalf the Grey]
    #26432525 - 01/14/20 03:21 AM (4 years, 1 month ago)

Ohhh Randalf! " and mag stirrer\hot plate " I love it when you talk dirty!  :doge:


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Offlineroarkell
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Re: Let's All Grow Mushrooms 2020 [Re: Inthepit]
    #26432566 - 01/14/20 04:49 AM (4 years, 1 month ago)

Lol... I've done brf cakes in the past but once I started with agar I definitely got the "white coat itch"... I agree tho, you dont need glass petris to be a scientist!


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OfflineRandalf the Grey
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Re: Let's All Grow Mushrooms 2020 [Re: roarkell] * 1
    #26432586 - 01/14/20 05:10 AM (4 years, 1 month ago)

Quote:

Inthepit said:
Ohhh Randalf! " and mag stirrer\hot plate " I love it when you talk dirty!  :doge:



Haha, just wait til I pull out my 10ml pipette.

^yeah, I don't know what that means either...

If you haven't tried them, I love the celltreat plastic plates. Biggest issue is disposal but I Just try to find other uses for them when I'm done.

Quote:

roarkell said:
Lol... I've done brf cakes in the past but once I started with agar I definitely got the "white coat itch"... I agree tho, you dont need glass petris to be a scientist!



Didn't help that I was doing mimosa hostilis extractions before I start mush cult so already had jars and bottles of liquid and powdered chemical. That's why I got the 10ml pipettes mentioned above. Great for pulling off that top layer. Throw in some beakers and stirrer, and all that goes with mush cult like syringes and SAB and sterility. I was hooked instantly.
Once I hit the agar phase (which was pretty early on in cult career) I was full on delusional. Feeling like professor Farnsworth from universe 420.
:goodnews:

I know some of you are legitimate mycologists and other biology or scientific fields and I don't want to take away from that or imply that I am on that level. I am not. But I love learning and knowledge and all that goes along with this and I'll continue to play scientist every chance I get.


--------------------
Randalf the Grey
Lost in the right direction
A wizard is never late..........but I can try
Randalf's Book of Genesis: In The Beginning - Teks, ratios, recipes and log entries
LAGM2O2O Grow Log
Genesis II: Electric Boogaloo - The Restart


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OfflineRumpRanger
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Re: Let's All Grow Mushrooms 2020 [Re: Randalf the Grey]
    #26432594 - 01/14/20 05:23 AM (4 years, 1 month ago)

:takingnotes:


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OfflineA.k.aM
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Re: Let's All Grow Mushrooms 2020 [Re: RumpRanger]
    #26432696 - 01/14/20 07:47 AM (4 years, 1 month ago)

Randolf I used to run mescaline and lsa extractions and whenever I had a step where you only wanted the top layer of liquid I always wished I had one of those badass glass funnel things you could drain the lower stuff with.

Pipette is def the best way but got too tedious for me so I would just use non corrosives so I could put it in a bag then once it had separated snip a little bit of a bottom corner off to let it leak out.


In other news it looks like my rw has germination, completing the set!  Toc is by far the fastest so far.


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LAGM2020


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