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OfflineKizzle
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Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 7 days
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Re: Cloning to grain [Re: Psilosion]
    #26391887 - 12/20/19 04:40 AM (5 years, 29 days ago)

Quote:

exactly what cron said the only reason it even worked for me was cuz it was a tissue sample from the inside of a fruit. i do not advocate even doin that ever i only did it cuz i was in a pinch and the fact it worked was prolly mostly luck. always use agar if you can otherwise be prepared for failure cuz most likely thats what will happen if your taking a fruit from the fc.




It's not luck. If you do it correctly inner mushroom tissue will produce a clean culture more often than not, even straight to grain.


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Offlinejcm4620
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Re: Cloning to grain [Re: Kizzle]
    #26391907 - 12/20/19 04:54 AM (5 years, 29 days ago)

ya i know that but its not a good procedure to follow is more so what im getting at. atleast i dont suggest doing it that way if it can be avoided. id rather be sire its clean by putting it on agar first.


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PANAEOLUS FRUITING MADE SIMPLE


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OfflinecronicrMFacebook
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Re: Cloning to grain [Re: jcm4620] * 1
    #26414659 - 01/03/20 04:12 PM (5 years, 14 days ago)



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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflinecronicrMFacebook
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Re: Cloning to grain [Re: cronicr]
    #26432157 - 01/13/20 08:45 PM (5 years, 4 days ago)



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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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InvisibleComebackKid
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Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cloning to grain [Re: cronicr]
    #26432232 - 01/13/20 09:25 PM (5 years, 4 days ago)

What variety is that cron?
Lookin good


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself

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OfflinecronicrMFacebook
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Re: Cloning to grain [Re: ComebackKid]
    #26432241 - 01/13/20 09:31 PM (5 years, 4 days ago)

A weird pe lol, it has tendency to drop spores, I actually received a PE print a few years ago and was suspect of it actually being pe but it's certainly pe and it yields very well, this isolate came from a monster I grew last year


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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InvisibleComebackKid
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Re: Cloning to grain [Re: cronicr]
    #26432249 - 01/13/20 09:37 PM (5 years, 4 days ago)

Neat lookin fruits.
I assume the potency is there eh


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself

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OfflinecronicrMFacebook
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Re: Cloning to grain [Re: ComebackKid]
    #26435086 - 01/15/20 02:29 PM (5 years, 2 days ago)


That middle fruit dropped a few spores


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflinecronicrMFacebook
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Re: Cloning to grain [Re: cronicr]
    #26437137 - 01/16/20 04:46 PM (5 years, 1 day ago)


Between the the five bottles I'll be over 2 ounces so from agar pin to a at to five muda bottles in just over a month.


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineEdmunter
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Registered: 05/01/13
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Re: Cloning to grain [Re: cronicr]
    #26652316 - 05/06/20 06:40 AM (4 years, 8 months ago)

Im trying this.  Ive loadsa plates that will pin soon

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OfflineA.k.aM
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Registered: 10/27/19
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Re: Cloning to grain [Re: Edmunter]
    #26652368 - 05/06/20 07:09 AM (4 years, 8 months ago)

Oh what.

It wouldn’t let me post in here yesterday.

Me too Ed I’ve got piles of plates with pins right now.


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LAGM2020

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Invisiblealaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 2,492
Loc: The 49th Dimension
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Re: Cloning to grain [Re: Kizzle]
    #26652501 - 05/06/20 08:43 AM (4 years, 8 months ago)

Quote:

Kizzle said:
Quote:

exactly what cron said the only reason it even worked for me was cuz it was a tissue sample from the inside of a fruit. i do not advocate even doin that ever i only did it cuz i was in a pinch and the fact it worked was prolly mostly luck. always use agar if you can otherwise be prepared for failure cuz most likely thats what will happen if your taking a fruit from the fc.




It's not luck. If you do it correctly inner mushroom tissue will produce a clean culture more often than not, even straight to grain.




Been my experience as well. :thumbup:  Also, cool post.  Nothing like beating the clock.


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THE 49TH MYCOJOURNAL
Oyster Buckets

FREE WILL IS A SHADOW

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