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mimir
Professional Noob

Registered: 01/21/19
Posts: 212
Loc: Europe
Last seen: 1 month, 17 hours
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LC Clumping
#26431683 - 01/13/20 03:53 PM (4 years, 1 month ago) |
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This is my first attempt at growing bulk. I have prepped around 17 pounds of WBS and have a lot of jars to inoculate (I wanted some leeway for errors, and WBS was pretty cheap). I have 5 clean Petris, which are obviously not going to be enough to inoculate all the jars in one go and I will have to make G2G transfers for sure. On the side, I have tried my hand at making LC, which you can see in the following pictures:

I have tested the LC and I'm still waiting on the results, but it seems to me that it might be clean. I have gotten little to no contaminants on agar so I'm hopeful. This will still not be my main source of inoculant, I don't wanna have to throw away all my grains, and I will only try it on a couple of small jars to see how it goes. My question is, when I tested it I could feel a large big clump in the center. How would you deal with this? My original plan was to pour the LC into the receiving jars, but I see some people like to use syringes to inoculate. I could also sterilize a scalpel or a long glass rod and manually break up the clump before pouring.
As to my recipe, I used the same recipe I use for PDA without the agar (20g/L of dextrose and potato broth from boiling potatoes).
Edited by mimir (01/13/20 04:02 PM)
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narmaduke
Stranger


Registered: 08/23/17
Posts: 197
Last seen: 1 year, 1 month
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Re: LC Clumping [Re: mimir]
#26431703 - 01/13/20 04:09 PM (4 years, 1 month ago) |
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your LC looks like you put agar in it
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 20 hours, 43 minutes
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Or it wasn’t ever shaken.
Lots of people use those magnetic stirrers.
Otherwise you’ll eventually get a mat on top like you’re starting to. The pics of shrooms growing on top of lc are pretty sweet though.
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LAGM2020     
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mimir
Professional Noob

Registered: 01/21/19
Posts: 212
Loc: Europe
Last seen: 1 month, 17 hours
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Re: LC Clumping [Re: A.k.a]
#26431745 - 01/13/20 04:44 PM (4 years, 1 month ago) |
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Quote:
narmaduke said: your LC looks like you put agar in it
I did not put any agar in it except for the wedge I used to inoculate
Quote:
A.k.a said: Or it wasn’t ever shaken.
Lots of people use those magnetic stirrers.
Otherwise you’ll eventually get a mat on top like you’re starting to. The pics of shrooms growing on top of lc are pretty sweet though.
I indeed have a mat of gelatinous stuff on top. I did shake it but I am using a polyfill lid and I couldn't shake it too hard or I would've risked wetting the filter. Is there any way to salvage it? The bottom part is still liquid. I will put something in it (marbles maybe) next time I try, until I can afford a magnetic stir plate
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: LC Clumping [Re: A.k.a]
#26431747 - 01/13/20 04:45 PM (4 years, 1 month ago) |
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Quote:
A.k.a said: Or it wasn’t ever shaken.
Lots of people use those magnetic stirrers.
Otherwise you’ll eventually get a mat on top like you’re starting to. The pics of shrooms growing on top of lc are pretty sweet though.
Hmm that sounds interesting... like shrooms growing on an island inside of an LC?
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