Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Buy Bali Kratom Powder, Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: < Back | 1 | 2 | 3 | Next >  [ show all ]
Invisiblerickyswamps
Bad Apple
I'm a teapot


Registered: 11/08/18
Posts: 1,192
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
    #26431702 - 01/13/20 04:09 PM (4 years, 1 month ago)

What kind of lids?  pics?


Extras: Filter Print Post Top
InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
    #26431704 - 01/13/20 04:12 PM (4 years, 1 month ago)

Quote:

smokedtrolli said:
I shook most of them after not seeing any mold and having some mycelium growth off of the wedge. Of the ones I did not shake, the contamination actually did originate from the top.




If you didn't see any growth (mushroom or otherwise) from anywhere but the top then you could probably forget the PC theory I suggested and assume it was from the lids or the agar or sloppy innlocation technique...

All u can do at this point is take some transfers from that slice u have left and tighten up your technique when you make your second attempt...

I think this is super weird... I've never seen 100% contamination rate before except for dumb shit like open air inoculated grain jars or something crazy like that..


I'm also very new to this as well so....


Extras: Filter Print Post Top
InvisibleStygianKnight
A Mushroom

Registered: 03/12/12
Posts: 2,717
Trusted Cultivator
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: SpunkyMonkey88]
    #26431762 - 01/13/20 04:56 PM (4 years, 1 month ago)

How old are those jars and how long after the shake?
Thatโ€™s an impressive amount of penicillium!


Extras: Filter Print Post Top
Offlinesmokedtrolli
Prologue
I'm a teapot User Gallery


Registered: 04/01/19
Posts: 224
Last seen: 1 day, 20 hours
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: StygianKnight]
    #26432951 - 01/14/20 10:37 AM (4 years, 1 month ago)

Quote:

StygianKnight said:
How old are those jars and how long after the shake?
Thatโ€™s an impressive amount of penicillium!




2-3 days after the shake, the jars are probably a week old. Pretty aggressive stuff.


--------------------
Money is too valuable to be moral.


Extras: Filter Print Post Top
Offlinesmokedtrolli
Prologue
I'm a teapot User Gallery


Registered: 04/01/19
Posts: 224
Last seen: 1 day, 20 hours
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: rickyswamps]
    #26432954 - 01/14/20 10:39 AM (4 years, 1 month ago)

Quote:

rickyswamps said:
What kind of lids?  pics?




1/4inch holes stuffed with polyfill. I'm not sure if that necessitates a picture or not considering half of the people who have grown w/ grains do the same thing. Nothing really special.


--------------------
Money is too valuable to be moral.


Extras: Filter Print Post Top
InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
    #26432958 - 01/14/20 10:41 AM (4 years, 1 month ago)

Quote:

smokedtrolli said:
Quote:

rickyswamps said:
What kind of lids?  pics?




1/4inch holes stuffed with polyfill. I'm not sure if that necessitates a picture or not considering half of the people who have grown w/ grains do the same thing. Nothing really special.




Ya that's pretty hard to fuk up 9/9times... weird dude.

I cant process how this is possible unless you licked your scalpel between jars or something


Extras: Filter Print Post Top
Offlinesmokedtrolli
Prologue
I'm a teapot User Gallery


Registered: 04/01/19
Posts: 224
Last seen: 1 day, 20 hours
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: SpunkyMonkey88]
    #26432960 - 01/14/20 10:44 AM (4 years, 1 month ago)

Quote:

SpunkyMonkey88 said:
Quote:

smokedtrolli said:
Quote:

rickyswamps said:
What kind of lids?  pics?




1/4inch holes stuffed with polyfill. I'm not sure if that necessitates a picture or not considering half of the people who have grown w/ grains do the same thing. Nothing really special.




Ya that's pretty hard to fuk up 9/9times... weird dude.

I cant process how this is possible unless you licked your scalpel between jars or something




I think it's my SAB actually. The bottom lacks uniformity so it isn't flush with the table, leaving space for air to enter under the SAB. That's my only guess. The colonized agar plate was left long enough for anything that wasn't mycelium to develop and reveal itself.

Probably gonna go buy a new container and make another SAB. And just be more rigorous with cleaning the work area before hand too.


--------------------
Money is too valuable to be moral.


Extras: Filter Print Post Top
InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
    #26432970 - 01/14/20 10:53 AM (4 years, 1 month ago)

Ya dude that sounds like a good plan. My table isn't uniformly flat either. It's made of 3 1' wide boards with a 1/4" gap in between them. So I lay down a big piece of plastic then a layer of paper toweling then i spray it down...

I've worked with just under 100 agar plates always pouring more than I need so they always sit for a week before using and I've only ever seen 1 plate contaminate before using it...

Just how I do shit and it works well for me...


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 1 day, 8 hours
Trusted Cultivator
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
    #26432975 - 01/14/20 10:56 AM (4 years, 1 month ago)

I have no real knowledge about this but it seems likely it has something to do with the grains. Maybe the jars werenโ€™t sterile?

If the agar was good that and the grains are really the only common denominators. I canโ€™t imagine it being the SAB, it seems like you could do it open air and not get that much/consistent contamination.

Definitely weird.


--------------------
LAGM2020


Extras: Filter Print Post Top
InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: A.k.a]
    #26432984 - 01/14/20 11:01 AM (4 years, 1 month ago)

Quote:

A.k.a said:
I have no real knowledge about this but it seems likely it has something to do with the grains. Maybe the jars werenโ€™t sterile?

If the agar was good that and the grains are really the only common denominators. I canโ€™t imagine it being the SAB, it seems like you could do it open air and not get that much/consistent contamination.

Definitely weird.




That's what I was saying at first but dude seems pretty confident he didnt fuk up the PC cycle...

He said he ran it for 1.5 hrs I always go for 2 so I dont know if that 30 mins could of left that contaminate in every jar or not...


Extras: Filter Print Post Top
Invisiblegizmo1
Other User Gallery


Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM Flag
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: SpunkyMonkey88]
    #26433017 - 01/14/20 11:45 AM (4 years, 1 month ago)

Quote:

SpunkyMonkey88 said:
Quote:

smokedtrolli said:
Quote:

rickyswamps said:
What kind of lids?  pics?




1/4inch holes stuffed with polyfill. I'm not sure if that necessitates a picture or not considering half of the people who have grown w/ grains do the same thing. Nothing really special.




Ya that's pretty hard to fuk up 9/9times... weird dude.

I cant process how this is possible unless you licked your scalpel between jars or something



Even licking the scalpel couldn't cause this. More likely that would cause a pretty bad case of bacteria.

OP 1.5 hours ain't bad but for oats I'd definitely increase to 2 hours and that also depends on your elevation.
Also I didn't see anyone ask did you vent your PC before building pressure? This is important.
I'd do another run. Stuff polyfil real tight, vent pc, and sterilize for 2 hours this time. Use the same culture but transfer to a new plate and post pics here so we can try to help you identify if anything is hiding in there.

With any luck you will have clean spawn in no time.


--------------------
Trade List
๐Ÿ–•๐Ÿ–•๐Ÿ–•
6 hole Mini Monos


Extras: Filter Print Post Top
Offlinesmokedtrolli
Prologue
I'm a teapot User Gallery


Registered: 04/01/19
Posts: 224
Last seen: 1 day, 20 hours
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: gizmo1]
    #26433095 - 01/14/20 12:53 PM (4 years, 1 month ago)

Quote:

gizmo1 said:
Also I didn't see anyone ask did you vent your PC before building pressure? This is important.





Considering I don't know what you mean by "vent", no, lol. Oops? I usually just everthing clean w/ bleach and soap before starting over again.


--------------------
Money is too valuable to be moral.


Extras: Filter Print Post Top
InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
    #26433109 - 01/14/20 01:01 PM (4 years, 1 month ago)

He means putting the jars in the PC and the lid on the PC but leaving the "jiggler/rocker" off while the steam vents out for 10 mins or so... they say that it is pretty important
.. I always do for that reason but I've also forgot and used to not vent until reading that with out any notable contaminations...


Extras: Filter Print Post Top
Invisiblegizmo1
Other User Gallery


Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM Flag
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: SpunkyMonkey88]
    #26433127 - 01/14/20 01:09 PM (4 years, 1 month ago)

Quote:

SpunkyMonkey88 said:
He means putting the jars in the PC and the lid on the PC but leaving the "jiggler/rocker" off while the steam vents out for 10 mins or so... they say that it is pretty important
.. I always do for that reason but I've also forgot and used to not vent until reading that with out any notable contaminations...



This just means you weren't pcing as long as you thought you were well not at temp anyways. If using a presto steam still escapes so you will eventually get the temps up but if you are only pcing for a hour and a half without venting then who knows how long you were actually at sterilization temps. You want only steam in the pc not steam and air pockets. The temperature of the air pockets will be much lower than the temperature of the steam. Always vent your PC.


--------------------
Trade List
๐Ÿ–•๐Ÿ–•๐Ÿ–•
6 hole Mini Monos


Extras: Filter Print Post Top
Offlinesmokedtrolli
Prologue
I'm a teapot User Gallery


Registered: 04/01/19
Posts: 224
Last seen: 1 day, 20 hours
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: gizmo1]
    #26433130 - 01/14/20 01:11 PM (4 years, 1 month ago)

Quote:

gizmo1 said:
Quote:

SpunkyMonkey88 said:
He means putting the jars in the PC and the lid on the PC but leaving the "jiggler/rocker" off while the steam vents out for 10 mins or so... they say that it is pretty important
.. I always do for that reason but I've also forgot and used to not vent until reading that with out any notable contaminations...



This just means you weren't pcing as long as you thought you were well not at temp anyways. If using a presto steam still escapes so you will eventually get the temps up but if you are only pcing for a hour and a half without venting then who knows how long you were actually at sterilization temps. You want only steam in the pc not steam and air pockets. The temperature of the air pockets will be much lower than the temperature of the steam. Always vent your PC.




Do you think that was the source of my penicillium tragedy?


--------------------
Money is too valuable to be moral.


Extras: Filter Print Post Top
Invisiblegizmo1
Other User Gallery


Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM Flag
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: smokedtrolli]
    #26433144 - 01/14/20 01:16 PM (4 years, 1 month ago)

Quote:

smokedtrolli said:
Quote:

gizmo1 said:
Quote:

SpunkyMonkey88 said:
He means putting the jars in the PC and the lid on the PC but leaving the "jiggler/rocker" off while the steam vents out for 10 mins or so... they say that it is pretty important
.. I always do for that reason but I've also forgot and used to not vent until reading that with out any notable contaminations...



This just means you weren't pcing as long as you thought you were well not at temp anyways. If using a presto steam still escapes so you will eventually get the temps up but if you are only pcing for a hour and a half without venting then who knows how long you were actually at sterilization temps. You want only steam in the pc not steam and air pockets. The temperature of the air pockets will be much lower than the temperature of the steam. Always vent your PC.




Do you think that was the source of my penicillium tragedy?



Can't say with 100% certainty but it could be. Not venting combined with low sterilization times could very well do it.


--------------------
Trade List
๐Ÿ–•๐Ÿ–•๐Ÿ–•
6 hole Mini Monos


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 1 day, 8 hours
Trusted Cultivator
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: gizmo1]
    #26433173 - 01/14/20 01:24 PM (4 years, 1 month ago)

Sounds likely.

Venting I always thought you let it run until a solid cone of steam was coming out.


--------------------
LAGM2020


Extras: Filter Print Post Top
InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: A.k.a]
    #26433237 - 01/14/20 01:53 PM (4 years, 1 month ago)

So a pressure cooker that wasn't vented but still reads X-psi is actually cooler than the same PC vented at the same PSI?


Extras: Filter Print Post Top
OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,234
Last seen: 3 hours, 48 minutes
Trusted Cultivator
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: SpunkyMonkey88]
    #26433251 - 01/14/20 01:57 PM (4 years, 1 month ago)

Quote:

SpunkyMonkey88 said:
So a pressure cooker that wasn't vented but still reads X-psi is actually cooler than the same PC vented at the same PSI?




A write up from Bod

The Importance of Venting a Pressure Cooker or Autoclave

BOD's Easy AF how to use your pressure cooker for mycology TEK


--------------------
All I need are some tasty waves, a cool buzz, and Iโ€™m fine.

Whatever you decide wonโ€™t really impact our survival
Close your eyes, and do the best that you can



Extras: Filter Print Post Top
InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: 9/9 oat jars contam'd with what appears to be Penicillium. Why? [Re: Camera93]
    #26433322 - 01/14/20 02:24 PM (4 years, 1 month ago)

Quote:

Camera93 said:
Quote:

SpunkyMonkey88 said:
So a pressure cooker that wasn't vented but still reads X-psi is actually cooler than the same PC vented at the same PSI?




A write up from Bod

The Importance of Venting a Pressure Cooker or Autoclave

BOD's Easy AF how to use your pressure cooker for mycology TEK




Seems like an interesting read I'm gonna check it out when I'm off work...


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | Next >  [ show all ]

Shop: PhytoExtractum Buy Bali Kratom Powder, Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* PF jar contams twice (now 3) in a row. upupup 5,821 18 05/30/19 02:12 PM
by Yowtzie
* Jar Contam and Pressure cooker Dilauded 1,452 4 12/28/02 01:09 PM
by Suntzu
* Post deleted by Moe Howard dog 1,013 4 06/29/02 10:47 AM
by Odd_Snail
* Rye jar contams. Joshua 2,551 6 01/22/02 03:49 AM
by MAIA
* -==No pinning in jars!==- CONTAMED!? arcadium 1,396 6 11/17/02 05:19 AM
by zeta
* bad smell, no visible contam Zebedee 2,841 4 02/24/02 02:06 AM
by Zebedee
* Post deleted by MOE THE MAD SCIENTIST
( 1 2 all )
vatoloco 10,090 21 04/30/02 03:27 AM
by Zebedee
* So I Opened a Jar......... Anonymous 525 3 01/23/03 09:53 AM
by SixCee

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,299 topic views. 29 members, 195 guests and 54 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.007 seconds on 14 queries.