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OfflineKnottedFungi
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Registered: 12/09/19
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Noob AGAR question
    #26430095 - 01/12/20 05:55 PM (4 years, 17 days ago)

I'm attempting to go from MSS to AGAR. I used pasty plates in a SAB with this MEA . I have no experience to justify my thinking but I don't think the directions(ratios) are good for spore germination or I could be needlessly second guessing myself. Does anyone have experience with this particular MEA, should my next batch have less MEA to make it less firm/more soupy?

The first batch of agar / pasty plates  I mistakenly used 3m transpore tape that melted, I waited 5 days for any signs of any growth which didn't happen so I ditched them. I made some more plates with polyfill and those are on day 3 with no signs of growth.

I guess I'm just being impatient.


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OfflineJohnRainy
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Re: Noob AGAR question [Re: KnottedFungi]
    #26430105 - 01/12/20 06:01 PM (4 years, 17 days ago)

The directions sound right. 

3 days isn't very long for spores.  That would be quick to see mycelium by then.


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Invisiblewildernessjunkie
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Re: Noob AGAR question [Re: KnottedFungi]
    #26430111 - 01/12/20 06:03 PM (4 years, 17 days ago)

You're being impatient.

Spores can take up to 3 weeks to germinate. Just keep waiting.

That agar is fine. I would ditch that tape, and you can replace it with Cling Wrap or just put the plate in a ziploc.


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Offlinerido
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Re: Noob AGAR question [Re: wildernessjunkie]
    #26430130 - 01/12/20 06:17 PM (4 years, 17 days ago)

Welcome to the forums!

I agree, 3-5 days isn't long enough to wait on spores to germinate. Plates are small enough that it doesn't hurt to keep them around longer, even if it's just to see how the growth progresses over time.


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OfflineKnottedFungi
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Re: Noob AGAR question [Re: rido]
    #26430835 - 01/13/20 08:37 AM (4 years, 16 days ago)

Thank you. I was being impatient. I checked my second batch of plates this morning and 3 out of 4 have the tiniest circular shaped growth pattern barely visible when held at an angle - the prepared MEA+colorant has a distinct sheen to it and the growth disrupts it.

I guess now I get to be impatient with finding out whether I'm growing something I want or not! Thank you again.


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Offlinerido
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Re: Noob AGAR question [Re: KnottedFungi]
    #26431751 - 01/13/20 04:48 PM (4 years, 16 days ago)

Quote:

KnottedFungi said:
Thank you. I was being impatient. I checked my second batch of plates this morning and 3 out of 4 have the tiniest circular shaped growth pattern barely visible when held at an angle - the prepared MEA+colorant has a distinct sheen to it and the growth disrupts it.

I guess now I get to be impatient with finding out whether I'm growing something I want or not! Thank you again.




That's great news! Fingers crossed that it's something good! I didn't see you mention it; what kind of prints are you using (paper or foil) and how are you transferring spores to the agar?


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OfflineKnottedFungi
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Re: Noob AGAR question [Re: rido]
    #26437595 - 01/16/20 10:13 PM (4 years, 12 days ago)

I started with a MSS of 'Blue Meanies'. Not Panaeolus cyanescens but the cubensis specimen by the same name. I didn't know there was contention with this variety or more so it's name until after I started this process.

Four of four plates have growth - I've made one transfer from one of the plates to another to isolate and am now waiting for that plate to show growth. I'm unsure what I'll do afterwards, I'm fairly confident I don't have enough experience to tell whether I'll need to further isolate or not but I've prepared a LC jar which will be the next step.


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OfflineKnottedFungi
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Re: Noob AGAR question [Re: rido]
    #26437664 - 01/16/20 11:11 PM (4 years, 12 days ago)

Quote:

rido said:
Quote:

KnottedFungi said:
Thank you. I was being impatient. I checked my second batch of plates this morning and 3 out of 4 have the tiniest circular shaped growth pattern barely visible when held at an angle - the prepared MEA+colorant has a distinct sheen to it and the growth disrupts it.

I guess now I get to be impatient with finding out whether I'm growing something I want or not! Thank you again.




That's great news! Fingers crossed that it's something good! I didn't see you mention it; what kind of prints are you using (paper or foil) and how are you transferring spores to the agar?




Oops to more directly answer your question not using prints, but spore syringe. To isolate I'm doing agar to agar in a SAB using a sterilized scalpel.


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