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HALUSN8
HALUSN8

Registered: 01/01/20
Posts: 7
Loc: Brisbane
Last seen: 4 years, 10 days
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Why are my jars contaminated only after shaking?
#26419961 - 01/06/20 08:13 PM (4 years, 1 month ago) |
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Hi guys, I just flushed my first batch of shrooms which weren’t a total failure. 2 of 4 tubs got contaminated but I still got a whole ounce dry so it could have been worse. Anyways the first batch of jars I was inoculating, I left in a clean tub with the lid on and no holes. I shook them once a week to mix them around to spread the mycelium. Now I’ve made another 9 jars. At first they were all fine until, I started shaking them up to mix them. Then they started growing large amounts of black stuff in side so I threw them. I had about 3 left. They were growing mycelium fine, until I shook these ones up and now they are starting to go black also. Should I leave them in a tub with the lid again to prevent containment’s? Why are they only starting to fuck out when I shake them to mix the grains?
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cli_hlt
Beshroomed



Registered: 10/29/11
Posts: 462
Loc: EU
Last seen: 1 month, 28 days
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Re: Why are my jars contaminated only after shaking? [Re: HALUSN8]
#26424064 - 01/09/20 07:23 AM (4 years, 1 month ago) |
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Grain jars need FAE, put some holes on the lids and fill/tape them with something (cotton wool, micropore tape etc.). The mycelium suffocates in a closed jar.
Contams need time to develop, there might be no connection with shaking, or shaking might just sped them up not *caused* contamination. Since there are no holes, no contamination could get in. (Right?)
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WeavieWonder
Time Travel Sucks



Registered: 08/18/16
Posts: 1,455
Loc: USA
Last seen: 1 month, 22 days
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Re: Why are my jars contaminated only after shaking? [Re: cli_hlt]
#26426584 - 01/10/20 01:36 PM (4 years, 1 month ago) |
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The problem with your jars is not where you are storing them. I store my colonizing jars on a shelf in a room. When grains dont recover after a shake, it means contamination is present. Whatever you inoculated that jar with was contaminated with mold. Other potential problems include improperly sterilized grains, or the filter on the jar is diffective.
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HALUSN8
HALUSN8

Registered: 01/01/20
Posts: 7
Loc: Brisbane
Last seen: 4 years, 10 days
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Re: Why are my jars contaminated only after shaking? [Re: cli_hlt]
#26427393 - 01/10/20 11:04 PM (4 years, 1 month ago) |
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There’s a hole in all my jars lids with poly fill...
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HALUSN8
HALUSN8

Registered: 01/01/20
Posts: 7
Loc: Brisbane
Last seen: 4 years, 10 days
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Re: Why are my jars contaminated only after shaking? [Re: HALUSN8]
#26427397 - 01/10/20 11:05 PM (4 years, 1 month ago) |
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Hmmmm I have no idea as I use a still air box. This is my second lot of inoculating the worst I did 8 jars not a problem. I’ll have another crack. This time I made jars from spore syringes I made not agar. See what happens
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cli_hlt
Beshroomed



Registered: 10/29/11
Posts: 462
Loc: EU
Last seen: 1 month, 28 days
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Re: Why are my jars contaminated only after shaking? [Re: HALUSN8]
#26427475 - 01/11/20 01:47 AM (4 years, 1 month ago) |
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Oh, I misunderstood that, I'm sorry.
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TheStallionMang
Do U know who yur fuckin with?


Registered: 10/18/17
Posts: 4,574
Loc:
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Re: Why are my jars contaminated only after shaking? [Re: HALUSN8]
#26428834 - 01/11/20 09:30 PM (4 years, 1 month ago) |
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Spore syringes are considered failry dirty. It's best to put it to agar to clean it up and then transfer it to grains. A SAB works fine for agar work. Check out this tek for agar dishes and prep
https://www.shroomery.org/forums/showflat.php/Number/19208976#19208976
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