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InvisibleJessicaRabbit88
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I'm a teapot User Gallery

Registered: 09/16/19
Posts: 78
Loc: Far Far Away
Re: Let's All Grow Mushrooms 2020 [Re: Randalf the Grey] * 1
    #26428343 - 01/11/20 03:19 PM (4 years, 1 month ago)

Extra precaution since I removed them from the SAB :shrug:


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OfflineRandalf the Grey
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Re: Let's All Grow Mushrooms 2020 [Re: footpath]
    #26428356 - 01/11/20 03:26 PM (4 years, 1 month ago)

I've seen some of you taking about trying some crosses and it got me thinking. This grow along has had me just as motivated and excited as when I got my very first spores. I'm going to hit the search bar when I get finished with some stuff but would love to hear some of you insight\teks\experience.

I'm thinking my RW would be a good option as it would give 2 visual indicators. I thought perhaps APE would be a good second option. Though it would negate the albino indicator of the RW, they are more easily identifiable in shape than my other option(Z, PFC, PR,CR,GT). So whatdaya think; white Rusty penis cross?
I have plenty of plates and agar and want to play around a bit.


--------------------
Randalf the Grey
Lost in the right direction
A wizard is never late..........but I can try
Randalf's Book of Genesis: In The Beginning - Teks, ratios, recipes and log entries
LAGM2O2O Grow Log
Genesis II: Electric Boogaloo - The Restart


Edited by Randalf the Grey (01/11/20 03:27 PM)


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OfflinePopaCap
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Registered: 07/23/18
Posts: 458
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Re: Let's All Grow Mushrooms 2020 [Re: footpath]
    #26428385 - 01/11/20 03:39 PM (4 years, 1 month ago)

Quote:

footpath said:
If I ever get a print of those, I'm definitely naming my culture Super PenESS.



I’ll be taking a bunch of swabs, so you’re more than welcome to one when it’s that time :super:


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InvisibleComebackKid
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Posts: 3,951
Loc: ked in the trunk of a car
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Re: Let's All Grow Mushrooms 2020 [Re: Asura]
    #26428459 - 01/11/20 04:23 PM (4 years, 1 month ago)

Quote:

Asura said:
Quote:

ComebackKid said:

You gunna do your cube grow the same way you do pans?






Not quite. I'll fruit right from the bag instead of moving to a tray.




Awh yeah good call.
Have you ever tried fruiting pans from the bag? or do you have to break it up cause of overlay or something?


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineJarhead3521S
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Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid] * 1
    #26428490 - 01/11/20 04:39 PM (4 years, 1 month ago)

Well I went out of town last night up to hunting lodge for a workday, took a space trip while we were hanging out and came home to all kinds of plates that decided to germ while I stepped out for the night! I’ll be headed back to the house after dinner to start making transfers and see if we can’t get some good growth going......seems to always happen when you turn your back for a little while.


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InvisibleJessicaRabbit88
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Registered: 09/16/19
Posts: 78
Loc: Far Far Away
Re: Let's All Grow Mushrooms 2020 [Re: footpath]
    #26428539 - 01/11/20 05:10 PM (4 years, 1 month ago)

Quote:

footpath said:
footpath's Grow Log

Species: Cubensis

Variety: Chitwan

Method: MS Syringe → Agar → Rye → Coir



Stuff & Things

  • Spores: Chitwan, Nepal  • Petri Dishes: Nest 100mm x 15mm  • Media Bottles: Cadamada 500mL  • Agar: Now Agar Powder  • LME: Breiss Light Dry Malt Extract

  • Knife: X-acto  • Torch: Bernzomatic WK2301








1 • 1 • 20

•  SporesAgar  •


I don't really bother streaking from a spore syringe. Figure more tools = more contam vectors. After wrapping it up, I'll usually gently tilt the plate around to carefully disperse the solution a bit.





1 • 3 • 20

•  Spore Germination  •



Blessed by some cat voodoo and voila! - fungus sex.





1 • 4 • 20

•  Spore Germination Progress  •



Just wanted to update to show the efficacy of simply just tilting around a droplet from a spore syringe as a method of skewing the culture about, hopefully acting as a pseudo-streaking.
Plus give big ups to the vendor I can't mention for clean AF syringes.





1 • 6 • 20

•  T1  •


Took some early transfers away from some probably bacteria.
Then I got kinda high and made that video :uhoh:





1 • 7 • 20

•  T1 Day 1  •




I don't usually take transfers that early. Got impatient, because social.
But I think there's some cute little strains a'formin' here...

1 • 8 • 20

•  T1 Day 2  •




Uploaded these in the same order as above. Didn't take them from the same angle, 'cause butts.
One hitchhiker (img2). Growth is pretty haywire, but relatively concentric. T2's for sure, but I'll probably end up throwing some of these to grain just to see.

1 • 9 • 20

•  T1 Day 3  •




Two of them were botch jobs.
That's what I get for taking transfers so early. :cookiemonster:


1 • 10 • 20

•  T1 Day 4  •




Moving along.
Wonder if I'm just transferring mold around...











I really like the size of your SAB.

Who makes it and what size is it?

Twice I've stopped myself from buying the Sterilite 110 qt tub because it just doesn't feel tall enough. But if BOD likes and uses it, no reason for me not to...


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Offlinefootpath
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Registered: 07/16/19
Posts: 1,367
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Re: Let's All Grow Mushrooms 2020 [Re: JessicaRabbit88]
    #26428565 - 01/11/20 05:30 PM (4 years, 1 month ago)

It is also the 110qt Sterilite. I'll have to add that to my Stuff & Things.

I wouldn't mind a bigger one.
If you get into ordering cases of petri dishes, they usually come in 20 or 25 stacks that are impossible to open upright in it.
It works, but it's a delicate juggle to unsheathe them.

Honestly, I see myself building a flowhood before upgrading to a bigger tote, however. So... it's obviously not that limiting.


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OfflineRandalf the Grey
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Re: Let's All Grow Mushrooms 2020 [Re: footpath]
    #26428590 - 01/11/20 05:46 PM (4 years, 1 month ago)

I have a 110 ish (I think like 112 or something) but not a sterlite. I think it's longer rather than taller. I works great except for bags and opening petri sleeves. Though, I have more often than not at this point opened the sleeves outside SAB and pulled plates out and quickly into SAB. This hasn't caused in contams as far as I can tell. I feel like opening in SAB is more risky just due to the amount of movement it takes.
Was thinking about using my old SAB and moding it to sit on its side just got bags.


--------------------
Randalf the Grey
Lost in the right direction
A wizard is never late..........but I can try
Randalf's Book of Genesis: In The Beginning - Teks, ratios, recipes and log entries
LAGM2O2O Grow Log
Genesis II: Electric Boogaloo - The Restart


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OfflineAsuraS
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Registered: 08/01/11
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Loc: Texas
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Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid] * 1
    #26428740 - 01/11/20 08:01 PM (4 years, 1 month ago)

Quote:

ComebackKid said:
Awh yeah good call.
Have you ever tried fruiting pans from the bag? or do you have to break it up cause of overlay or something?




I haven't. I think CaptainFuture has done it like that. The pans seem to like a shallow sub (2") and the yields
are better from a tray (based on pics of bag grows I've seen). It moves so quick that breaking it up
doesn't even make it skip a beat. It's ready to fruit 5 days or so from being put in a tray.


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OfflineCootermonkey
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Re: Let's All Grow Mushrooms 2020 [Re: Asura]
    #26428865 - 01/11/20 09:58 PM (4 years, 1 month ago)


First 2 are my Tex orange cap,the other crappy photos are my Pesa
noticed the growth last night but seein as i have em in my room and my 9 month pregnant wife was wanting to sleep and givin me "ima beat you looks" i decided it was wiser to turn off the lights and wait for today to take a couple pics hahaxD
I really like these mini ziploc screw tops though, only 4 come in the package but theyre sturdy little shits. Ima definetly buy more and just do away with my mini rounds and the square ones.
Im so happy to be able to cultivate again, i missed all this so much. Cant wait for the fruits of my relaxation to come in


--------------------
MyLAGM2020Growlog


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Offlinefootpath
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Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 3 months
Re: Let's All Grow Mushrooms 2020 [Re: Cootermonkey]
    #26428907 - 01/11/20 10:38 PM (4 years, 1 month ago)

1 • 11 • 20

•  T1 Day 5  •




I think three of five are now compromised. Maybe not, however.
I'll be happy if even one plate is good enough to take to grain.





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InvisibleYogiBear
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Registered: 08/24/19
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Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid]
    #26429094 - 01/12/20 03:30 AM (4 years, 1 month ago)

Quote:

ComebackKid said:
ComebackKid's Grow Log

JAN 10 2020
Things are really getting out of control now. I've got cultures out the ying yang over here in the germination station.
Put some Pan Cyan and Pan Cambo spores to agar today.


Trying something new. Thanks to Mary Fairchild for the gift :hug:




CBK... what type of containers are those for your pan knock ups? If they are PP5 I'd like the source please. 😍


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OfflineSamskara92
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Registered: 08/29/19
Posts: 334
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Re: Let's All Grow Mushrooms 2020 [Re: YogiBear]
    #26429247 - 01/12/20 07:26 AM (4 years, 1 month ago)

Took three transfers from both KSSS plates last night, will update log and post pictures later this evening


--------------------
:jellyfish: We're all just Motion in the Dust:jellyfish:


LAGM 2020 Grow Log - KSSS
CBK's Proper Surface Moisture/ FAE Tek


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Invisibleeatyualive
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Posts: 19,026
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Re: Let's All Grow Mushrooms 2020 [Re: Samskara92]
    #26429390 - 01/12/20 09:49 AM (4 years, 1 month ago)

I got leap off on my liquid inoculate.



Ive actually fruited pans invitro in spawn bags that have a filter patch on them.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


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OfflineJarhead3521S
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Re: Let's All Grow Mushrooms 2020 [Re: eatyualive] * 1
    #26429429 - 01/12/20 10:15 AM (4 years, 1 month ago)

1/12/20: T1 transfers on TOC and PESA, isolation T3 on a GT plate I’ve been messing with from MS.  I’ve got 2 of the original 5 PE6 plates left to pull transfers on, wanted to let them grow out a bit more first.




--------------------


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OfflineTheWitchfinder

Registered: 08/28/13
Posts: 76
Loc: Salem
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Re: Let's All Grow Mushrooms 2020 [Re: Jarhead3521]
    #26429452 - 01/12/20 10:27 AM (4 years, 1 month ago)

I think I am ready to make my first transfers. The AA+ swipes are looking great. I'm working on getting pictures uploaded/OP updated.


--------------------
TheWitchfinder's LAGM2020 Log


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InvisibleComebackKid
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Re: Let's All Grow Mushrooms 2020 [Re: Jarhead3521]
    #26429453 - 01/12/20 10:27 AM (4 years, 1 month ago)

Yogi

I ordered the wrong ones but this is the thread for holy grail no pour dishes


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineRandalf the Grey
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Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid]
    #26429478 - 01/12/20 10:47 AM (4 years, 1 month ago)

Quote:

ComebackKid said:
Yogi

I ordered the wrong ones but this is the thread for holy grail no pour dishes




Those for real look like the holy Grail of no pours. I may actually give no pours another shot with those.
Will likely burn through the rest of my petris during this grow along.


--------------------
Randalf the Grey
Lost in the right direction
A wizard is never late..........but I can try
Randalf's Book of Genesis: In The Beginning - Teks, ratios, recipes and log entries
LAGM2O2O Grow Log
Genesis II: Electric Boogaloo - The Restart


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OfflineA.k.aM
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Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid]
    #26429482 - 01/12/20 10:49 AM (4 years, 1 month ago)

Is there any reason not to take a transfer from a spot that just germinated? I have a bunch of spots that are by themselves that are still barely visible.


--------------------
LAGM2020


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Offlinefootpath
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Posts: 1,367
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Re: Let's All Grow Mushrooms 2020 [Re: A.k.a]
    #26429494 - 01/12/20 10:58 AM (4 years, 1 month ago)

Something else may not have germinated yet.

edit: or replicated enough to observe.


Edited by footpath (01/12/20 11:02 AM)


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