Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules

Jump to first unread post Pages: 1
OfflineNewbiegrower123
Spiritual enthusiast
Female

Registered: 02/08/19
Posts: 17
Loc: Québec, Canada Flag
Last seen: 2 months, 12 days
Failure after failure
    #26422400 - 01/08/20 06:20 AM (4 years, 1 month ago)

Hello fellow mushrooms enthusiasts!

I have grown Psylocibe Cubensis once in the past and it worked. I'm starting to think it was the beginner's chance...

I have tried a couple of times in the past few months to grow different strains (Space Coast, Blue Meanie, Stropharia) and also the latest one; Golden Teacher

For the first ones, I had waited 2 months to inoculate my jars with the syringes and figured they might had gone bad so I tried again with Golden Teacher. It has been more than 10 days now since I inoculated and no mycelium. I need someone to tell me what I'm doing wrong!

Here is how I proceeded;

I ordered two spore syringes by mail. I received them but since it's winter here, they froze in the mailbox before I got to them. I then thawed them slowly and inoculated them the next day. I used the two syringes for 12 half pint jars, one of them seemed to have the spores stuck on the side of the needle so I only inoculated 4 jars with it. (Am I using too much spore liquid per jar? Is it normal that I could see the black spores in the liquid?)

I used BRF tek with a ratio of 2 parts verm, 1 part BRF and 1 part water. I sterilized them for 90 minutes and let the jars cool overnight in the pot. (I don't think I did anything wrong during this process)

Here is where I think there is a problem;
My jars are in the basement where the wood stove is (we heat up the house with it). Now the only activity in my jars is some yellowish humid thing, aligned with the holes in my lids. Is it too dry because of the wood stove?

I don't regulate the temperature in the basement, sometimes it's too cold I think and sometimes maybe too hot. (I read that the yellowish thing might be "mycelium piss" because of the heat)

PLEASE HELP ME! I AM DESPERATE!

Thank you so much, I love you guys! <3

(I can post pictures of my jars later)


--------------------
Your friend


Extras: Filter Print Post Top
OfflineThe Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 13 days, 27 minutes
Re: Failure after failure [Re: Newbiegrower123]
    #26422407 - 01/08/20 06:32 AM (4 years, 1 month ago)

Sometimes germination times can take a bit.

Colder temps may play a roll.

The only way to get out of that guessing game is to use agar.

Varieties (golden teacher etc) dont really have a bearing on method.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


Extras: Filter Print Post Top
OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery

Folding@home Statistics
Registered: 05/12/07
Posts: 69,595
Loc: The Inexpressible... Flag
Last seen: 7 hours, 5 minutes
Trusted Cultivator
Re: Failure after failure (moved) [Re: Newbiegrower123]
    #26422430 - 01/08/20 07:01 AM (4 years, 1 month ago)

This thread was moved from The Pub.

Reason:
Mush Cult is here to help :thumbup:


Extras: Filter Print Post Top
OfflineSocrateshroom
сталкер


Registered: 09/05/18
Posts: 1,840
Loc: Westworld
Last seen: 1 month, 4 days
Re: Failure after failure [Re: Newbiegrower123] * 2
    #26422515 - 01/08/20 08:26 AM (4 years, 1 month ago)

Post pics of your jars.

I've only experienced a real issue once (and it was due to improper and lengthy storage of a syringe) which lead to no growth (most likely inviable spores at that point).

The Mycologist is right, agar work is the standard for doing this. (and also that growth sometimes takes a while)

But if your like me and you like brf jars :hehehe: you need to go back, step by step, and check your process.

Did you follow the PF Tek 2.0 guide exactly?

Should look something like this

  • Have clean jars or wash/dry jars.

  • Fill jars with your mixture of 2 parts verm/1brf/1water (make sure you spent a good time thoroughly mixing it all together.)and your dry verm layer then foil them up.

  • Immediately after filling all jars, sterilize in pot or pressure cooker (I personally suggest a pressure cooker. I've never done it any other way and I've never had a problem. I always did it for 60-90 mins without issue)

  • Let cool overnight (or minimum 8 hours)

  • When you are ready to remove from your pressure cooker/pot, prep your area. I wash everything down with 70% ISO. Get your Still Air Box ready (wash the entire thing down with iso). I also saw a suggestion that you should spray the walls with a soapy mixture since debris and such will stick to that. I've always done that but I don't know if it is actually necessary. Get your nitrile or such gloves on, wash them down with the iso.

  • Get your jars out, into your still air box and inoculate. Inoculate as close to the wall of the jar as you can. You should see liquid squirting against the wall of the jar. Put the jar aside (inside the SAB) and repeat until done. I let my jars sit in my still air box while I clean up outside the SAB. Then onto a shelf and wait.


Some things I've noticed:

  • I've inoculated dead center before as an experiment. That took forever to see growth because they germinated from the center and worked their way out. That's why I now do it basically against the glass, so I can track it better.

  • 0.5cc of liquid per inoculation point (1cc per jar) is more than enough. You can get away with less, I just have a heavy finger.



And, to tackle some things:

Quote:

Newbiegrower123 said:

I used the two syringes for 12 half pint jars, one of them seemed to have the spores stuck on the side of the needle so I only inoculated 4 jars with it. (Am I using too much spore liquid per jar? Is it normal that I could see the black spores in the liquid?)






As above, a 10cc syringe of solution should be enough for at least 10 jars. So 2 syringes should be good for at least 20 jars.

Did you see the clump of spores? Hold the syringe up to the light and see if it is clear or if there are spores floating. If there us a clump (which happens when the syringe is stationary) just vigorously shake to break it up.

Make sure you are buying from sponsored vendors. And you mentioned that they froze? Perhaps that is the issue. How did you thaw them? Check with someone more knowledgable whether the freezing or your thawing process was enough to kill the spores.


Quote:

Newbiegrower123 said:
Here is where I think there is a problem;
My jars are in the basement where the wood stove is (we heat up the house with it). Now the only activity in my jars is some yellowish humid thing, aligned with the holes in my lids. Is it too dry because of the wood stove?

I don't regulate the temperature in the basement, sometimes it's too cold I think and sometimes maybe too hot. (I read that the yellowish thing might be "mycelium piss" because of the heat)






We need pictures to see the yellowish thing you speak of.

The ideal growth temperature for the jars is between 68-75 Fahrenheit (At least that has worked for me). Make sure to check with a trusted cultivator if that temperature range is correct. I read that anything below can stall growth and above 80ish benefits the growth of bacteria. I'd suggest a thermometer or hygrometer to keep an eye on the temperature there.


--------------------


Edited by Socrateshroom (01/08/20 08:28 AM)


Extras: Filter Print Post Top
OfflineLookingLurker
Stranger
Registered: 01/03/20
Posts: 47
Last seen: 1 year, 3 months
. [Re: Socrateshroom]
    #26422547 - 01/08/20 09:01 AM (4 years, 1 month ago)

.


Edited by LookingLurker (06/21/21 10:05 PM)


Extras: Filter Print Post Top
OfflineNewbiegrower123
Spiritual enthusiast
Female

Registered: 02/08/19
Posts: 17
Loc: Québec, Canada Flag
Last seen: 2 months, 12 days
Re: Failure after failure [Re: LookingLurker] * 1
    #26422685 - 01/08/20 10:18 AM (4 years, 1 month ago)

Thank you so much guys, I will process all this info tonight and see what I will do.


--------------------
Your friend


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 14 hours, 10 minutes
Trusted Cultivator
Re: Failure after failure [Re: Newbiegrower123] * 1
    #26422703 - 01/08/20 10:27 AM (4 years, 1 month ago)

I’m curious what they say about freezing.

Make sure you shake them up really well before using them too. I’ve had a couple that were a pain in the ass. Started as one giant ball and only wanted to break into three smaller ones until like five minutes of shaking them.


--------------------
LAGM2020


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,865
Last seen: 17 hours, 12 minutes
Trusted Cultivator
Re: Failure after failure [Re: A.k.a] * 2
    #26423180 - 01/08/20 03:34 PM (4 years, 1 month ago)

Freezing spores in a liquid damages them. But it's worth noting people have successfully used frozen syringes. As for the conditions in your basement the cold can definitely slow down germination. The dry air shouldn't be an issue since they're in a jar and not a lot of time has passed. If you're seeing condensation suddenly appearing it may be a sign something is growing.


--------------------


Extras: Filter Print Post Top
OfflineNewbiegrower123
Spiritual enthusiast
Female

Registered: 02/08/19
Posts: 17
Loc: Québec, Canada Flag
Last seen: 2 months, 12 days
Re: Failure after failure [Re: Kizzle]
    #26425186 - 01/09/20 06:12 PM (4 years, 1 month ago)

Tomorrow will be the two weeks deadline and I just checked them and nothing...

I  live in Quebec, Canada and I need to find a vendor that ships here and who will be able to send my spores without them freezing. I can't seem to find anything interesting in the sponsors list!

Maybe I should try reddit? Maybe there is a better way to grow them? is it more complicated with prints?

If anyone knows a better tek than the BRF cakes please let me know... I looked up agar and it seems way more complicated. I was also thinking of making an incubator that could control temperature and humidity so if you got any ideas I'm open to anything!

I'll keep looking in other threads as well

Thank you guys so much


--------------------
Your friend


Extras: Filter Print Post Top
InvisibleDigit
Surreal Estate Agent
Registered: 08/10/15
Posts: 69
Loc: tierra incognita
Re: Failure after failure [Re: Newbiegrower123]
    #26425261 - 01/09/20 07:02 PM (4 years, 1 month ago)

I had some syringes that got accidentally frozen in my fridge and they were as dead as dead gets.
You can get a print instead, but then you'd need to start with agar as well, which as you say is a lot more complicated.
However agar is the "right" way to do things (arguably) and very much worth learning how to do if you intend to pursue this hobby further.  You'll also have a much higher success rate, eventually, if you take the time to learn about it.
As far as temperature control you definitely should keep your jars within the 68-78 farenheit range, whatever that is in degrees C. I like to keep mine on the warm side of the range for faster growth. Make an incubator from an insulated beer cooler or an old mini-fridge if you can't heat the space with a regular heater. There are plenty of teks out there for heated incubators. I made one from a mini-fridge with a low-wattage light bulb inside it, on a thermostat. It works great.

Best of luck


--------------------
Question everything.
Believe nothing.
Think for yours3lf.


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 14 hours, 10 minutes
Trusted Cultivator
Re: Failure after failure [Re: Digit]
    #26425302 - 01/09/20 07:28 PM (4 years, 1 month ago)

If you get a print you could sterilize the syringe and then put the print in it and avoid agar.

Agar is awesome and so much easier than it seems, but I totally understand not wanting to go that route. I wanted nothing to do with it until I was a few grows deep and hooked.

All the Petri pictures used to annoy the shit out of me now I can’t get enough.


--------------------
LAGM2020


Extras: Filter Print Post Top
InvisibleF-Bomb
Stranger
I'm a teapot User Gallery
Registered: 10/28/19
Posts: 119
Re: Failure after failure [Re: Newbiegrower123]
    #26425354 - 01/09/20 08:04 PM (4 years, 1 month ago)

Quote:

Newbiegrower123 said:
.....spores freezing. I can't seem to find anything interesting in the sponsors list!

Maybe I should try reddit? Maybe there is a better way to grow them? is it more complicated with prints?



The postal service offers low price climate controlled secure boxes inside of most offices, as do many other private companies. The postal service rules are more strict regarding (non)opening of packages.

Reddit can be a valuable tool, lots of spore trading happening there.

If you grow from a print you'll need to either make a syringe yourself or scrape spores onto agar from the print.  Keep in mind that prints are (IMO) always dirty so there's still a good chance you'll experience issues with MS solution to jars. 

I make agar in the microwave in 15 mins or so, it's really painless and there's lots of information about doing it all over the web.  You'll get to see the entire process up close on the plate, selecting and transferring the "best" parts to another plate to isolate, etc... The science behind everything is fascinating to me so it keeps me coming back.

A sleeve of 60mm plates is $5 or less in most cases, and a pouch of agar is a few pennies.  You can use leftover water from boiling grain (from making jars) as the nutrient liquid to make your agar. 

With agar you can make a single print or syringe last quite a long time.  Just maintain your cultures on plates or in slants (tubes) in a refrigerator.  Sealed with parafilm they can live for a couple years before you need to renew them.


Extras: Filter Print Post Top
OfflineNewbiegrower123
Spiritual enthusiast
Female

Registered: 02/08/19
Posts: 17
Loc: Québec, Canada Flag
Last seen: 2 months, 12 days
Re: Failure after failure [Re: F-Bomb] * 1
    #26427715 - 01/11/20 08:51 AM (4 years, 1 month ago)

I think you guys convinced me to try agar...

I'll look into that!

Thank you so much I was desperate and needed these kind pieces of advice


--------------------
Your friend


Extras: Filter Print Post Top
OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery


Folding@home Statistics
Registered: 05/12/07
Posts: 69,595
Loc: The Inexpressible... Flag
Last seen: 7 hours, 5 minutes
Trusted Cultivator
Re: Failure after failure [Re: Newbiegrower123]
    #26427866 - 01/11/20 10:41 AM (4 years, 1 month ago)

If u havent read this mega-thread, i highly recommend reading it. Lots of excellent links to read: https://www.shroomery.org/forums/showflat.php/Number/26011749


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules


Similar ThreadsPosterViewsRepliesLast post
* Temperature Variation Theory ExtravagantDream 3,400 3 07/08/03 01:33 PM
by ExtravagantDream
* Temperatures Vhal 1,225 7 04/04/05 07:26 PM
by Vhal
* Colonization Temperature Alice_Bodut 7,083 3 09/07/02 05:48 PM
by SixTango
* Plans for my terarrium marx2k 908 2 07/20/01 09:24 PM
by aluminum_can
* Rye water problem
( 1 2 all )
Damn_Skippy 4,215 26 02/08/09 12:32 PM
by Roggen_Rocker
* MAJOR BREAKTHROUGH - FASTER PINNING VIA TEMPERATURE CYCLING
( 1 2 all )
Hannibal88 18,183 25 07/08/07 09:29 AM
by RogerRabbit
* basement greatlakes 1,215 8 08/21/05 09:20 PM
by hyphae
* ceramic space heater for fruiting in basement? sup3rhuman 1,777 5 06/19/06 10:05 AM
by 2FiNiTe

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
482 topic views. 31 members, 157 guests and 42 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.006 seconds on 12 queries.