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OfflineA.k.aM
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Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid]
    #26426936 - 01/10/20 05:13 PM (4 years, 1 month ago)

Does nutrient content matter for spore plates or just softness?

I know for spores 10 days isn’t that long but I have so many plates swiped i feel like something should’ve grown by now.


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InvisibleComebackKid
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Re: Let's All Grow Mushrooms 2020 [Re: A.k.a]
    #26426955 - 01/10/20 05:26 PM (4 years, 1 month ago)

I like to be on the nutrient rich side for germination although I doubt that's really making much of a difference in your case.
10 days isn't uncommon my guess would be they're just stubborn


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

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Offlinetealseal
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Re: Let's All Grow Mushrooms 2020 [Re: A.k.a]
    #26427014 - 01/10/20 06:13 PM (4 years, 1 month ago)

Quote:

A.k.a said:
Does nutrient content matter for spore plates or just softness?

I know for spores 10 days isn’t that long but I have so many plates swiped i feel like something should’ve grown by now.




Mine are just now looking transferable and im at 10 days. I may give it a day or 2 but id say its pretty normal..


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OfflineRandalf the Grey
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Re: Let's All Grow Mushrooms 2020 [Re: tealseal]
    #26427025 - 01/10/20 06:20 PM (4 years, 1 month ago)

I have noticed that most people on here seem to use a one-to-one ratio for agar to nutrients. I'm using a two-to-one ratio of nutrients to agar. That is 7.5 grams of agar and 15 grams of malt extract to 500ml of water and seem to get pretty quick germ on all my plates (except cyans because they hate me). Most recent was my z strain that had visible growth on day 3


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Randalf the Grey
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Randalf's Book of Genesis: In The Beginning - Teks, ratios, recipes and log entries
LAGM2O2O Grow Log
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OfflineNikoyo
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Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid]
    #26427029 - 01/10/20 06:21 PM (4 years, 1 month ago)

Quote:

ComebackKid said:
Going to lose my mind trying to keep after all these cultures pretty soon tho :lol:



Tell me about it, Ive got 7 other projects in the works ontop of the two going on for this :rush:


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InvisibleSmartattack
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Re: Let's All Grow Mushrooms 2020 [Re: Nikoyo]
    #26427091 - 01/10/20 06:49 PM (4 years, 1 month ago)

Thinking one or two more days I'm going to drop one plate to LC and other one to master jar. I already know this myc loves my LC as the stuff I started on Christmas has already formed huge mass.

:nowaydude:


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InvisibleComebackKid
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Re: Let's All Grow Mushrooms 2020 [Re: Nikoyo] * 2
    #26427114 - 01/10/20 07:00 PM (4 years, 1 month ago)

Quote:

Nikoyo said:
Quote:

ComebackKid said:
Going to lose my mind trying to keep after all these cultures pretty soon tho :lol:



Tell me about it, Ive got 7 other projects in the works ontop of the two going on for this :rush:



It's amazing how fast the myc colonizes your brain and starts controlling your thoughts and actions.
Only just got back into this hobby recently and I told myself I was going to keep it scaled down... couple months later I'm already thinking of adding another shelf and building a GH :facepalm3:

Tis a slippery slope



This could possibly help with germination.
I started digging the tip of my swab into the soft agar to see what happens. (Usually just swipe the surface)
I have a few pics.

These two are the germ plates from this thread.
First plate I swiped on the surface of the agar. The second I swiped in and dug a spot into the agar on the left.

The spot where I dug the swab in had germination first...


This is a P cambo swab from an old baggie that I didnt even know I had till a couple weeks ago.


she's a real beauty eh? :facepalm:

Swiped the surface 17 days ago and then dug the swab into the agar... and that's where the germination happened.


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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Offlinetrubblesome
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Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid] * 1
    #26427137 - 01/10/20 07:12 PM (4 years, 1 month ago)

Update 1/10:

both my LC test plates came up clean, so yesterday I took the remainder of the LC, decanted most of the liquid off the top, and dumped about 15 ml of nicely developed snot balls of liquid myc on to two new jars, one for each LC. then I took a sample from the remainder and observed it under the scope just to verify it was still clean. It looked good, but I gram stained it anyway to help me better see any bacteria that might be present. while doing that I got this pic of what I think is a clamp connection at 400x:




honestly I think the pours from yesterday will do better than the ones I poured too early, it's way more vigorous and is already showing signs of growth on the oats, the ones I poured too early are only just now showing growth. might have shot myself in the foot there trying to get a jump start! 

I also took a step back and realized that I'm probably taking on too much at once, and fucked around in my dishes too much to be actually tracking them waiting for a single spore to germ before contamination took over. so I swiped new dishes specifically for that project and I'll be growing nice looking transfers out like normal from the two transfers I already took. To be honest it's probably a waste of time and doing a fresh streak from whatever I harvest next will probably produce a single spore germination faster than the print I have. but I did it anyway because I was curious about trying out a weird method of spore application. it wasn't great but you know, not everything works: 



for the regular streaks I lightly drew my loop across some of the areas of my print with a finer spore load, then carefully turned the loop so that those spores were facing "up" on the loop. Then I streaked the plate with the bottom side of the loop touching the agar, so that the movement would more lightly draw spores off the top of the loop and increase my chances of finding single spore locations to cut from later. These were very sucessful, I found several locations with individual spores I'll be cutting out to move when I have time.

The less successful plates, labeled "scatter," I lightly drew the loop on the spore print in the same manner, but then I held the loop about 4" above the plate, and used the tip of a flame sterilized scalpel to pull back the loop and then let go, the idea being a scatter of spores would be propelled on to the plates and I could find numerous locations with single spores in easy to pluck locations. I don't think I let the plate sit long enough before moving my hands and putting the lid back on for any spores except the larger clumps to land on the surface of the agar, and scanning the surface of the plates for spores that could be literally anywhere ended up not being very fun and kind of straining on the eyes so I'll be focusing on the light streaking method for now, it works fine enough.


from the grocery store swabs, I have growth!!



everything but the maitake germinated, which is what I guessed would happen. I don't even think there are any maitake spores on that plate. I'm working the maitake in a clone method from Tradd Cotter where it's dunked underwater with just the base exposed to force growth out of the soft tissue at the base. no spores. I made a separate journal for these where I go in to that in more detail: Growing grocery store mushrooms

That's all for now - I think I'm at the point where I can just sit back and watch for a little while.




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InvisibleHobbit GDF
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Re: Let's All Grow Mushrooms 2020 [Re: trubblesome] * 1
    #26427310 - 01/10/20 09:45 PM (4 years, 1 month ago)

So I updated OP. I am going to let the transfers grow out a little bit before next transfers.


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OfflineSFS96
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Re: Let's All Grow Mushrooms 2020 [Re: Hobbit GDF] * 1
    #26427320 - 01/10/20 09:54 PM (4 years, 1 month ago)

Quote:

ComebackKid said:
Quote:

SFS96 said:
Finally did my first ape transfers.






Awh yee
Besides the bacteria the growth is lookin pretty nice eh? Making me want to grow some ape again soon



Yeah I’m pretty happy how the growth is looking. I think the 3 transfers I made from this plate are going to do great. I have 2 more ape plates I need to transfer from just haven’t had time today. Iv been busy constructing a hood :rockon:


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How I make and preserve tea


Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood


Edited by SFS96 (01/10/20 09:58 PM)


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OfflineAsuraS
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Re: Let's All Grow Mushrooms 2020 [Re: SFS96]
    #26427373 - 01/10/20 10:33 PM (4 years, 1 month ago)

I finally have some shit going down. Did T1 transfers of pan jam and aussie tonight.
My one plate of z-strain is starting to move.

My plan is to catch up to you guys a bit and take that z-strain to LC and then do
a direct inoculation to sterile sub in a myco bag.


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Offlinejcm4620
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Re: Let's All Grow Mushrooms 2020 [Re: Asura]
    #26427418 - 01/10/20 11:49 PM (4 years, 1 month ago)

damn asura i wish id have known u were gnna do some z id have been more than happy  to send u a print  from the ones i just did


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OfflineInthepit
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Re: Let's All Grow Mushrooms 2020 [Re: DcevLearn]
    #26427460 - 01/11/20 01:10 AM (4 years, 1 month ago)

DcevLearn said:
Quote:

Luckily kept good notes
  • The S plate is 1.4% SAGARI 125ml H20 / 1.75g AGAR / 1.75g LME
  • The L plates are 0.6% Low Nute 125ml H20 / 2.5g AGAR / 0.75g LME




  • Are you back yet?
    I just have a quick question. I'm going to try SAGARI method, so I'm confused by "1.4% sagari". Can you expand on that a little more?

    For the Grow Logs it's really cool for noobs like meeee to see details like these agar recipies!  :popcorn:


    Edited by Inthepit (01/11/20 01:19 AM)


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    OfflineInthepit
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    Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid]
        #26427502 - 01/11/20 02:50 AM (4 years, 1 month ago)

    Are people preferring a swab over an inoculation loop?
    So far the only advantage, I read about, is not introducing more water from LC or syringe. Otherwise a loop seems more straight forward.

    Quote:

    ComebackKid said:
    This could possibly help with germination.
    I started digging the tip of my swab into the soft agar to see what happens. (Usually just swipe the surface)
    I have a few pics.

    These two are the germ plates from this thread.
    First plate I swiped on the surface of the agar. The second I swiped in and dug a spot into the agar on the left.






    --------------------
    :sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


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    OfflineA.k.aM
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    Re: Let's All Grow Mushrooms 2020 [Re: Inthepit] * 1
        #26427557 - 01/11/20 05:18 AM (4 years, 1 month ago)

    Pretty sure I finally got something happening on a couple of the softer plates I streaked a few days in....so excite.


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    OfflineOne of Us
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    Re: Let's All Grow Mushrooms 2020 [Re: A.k.a] * 1
        #26427598 - 01/11/20 06:14 AM (4 years, 1 month ago)

    1/11/2020: Here is where we are today.

    Golden Halo t1's



    Malaysian t1.  It has a nice rhizo section that I will pull from in the next day or two


    Here is a malaysian germ plate that has only one colony (and no contams!):


    Gymnopilus luteofolius t1.  I am not sure if this looks right. Definitely different from psilocybe mycelium


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    LAGM 2021
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    InvisibleComebackKid
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    Re: Let's All Grow Mushrooms 2020 [Re: Inthepit] * 1
        #26427660 - 01/11/20 07:43 AM (4 years, 1 month ago)

    Quote:

    Asura said:
    I finally have some shit going down. Did T1 transfers of pan jam and aussie tonight.
    My one plate of z-strain is starting to move.

    My plan is to catch up to you guys a bit and take that z-strain to LC and then do
    a direct inoculation to sterile sub in a myco bag.




    You gunna do your cube grow the same way you do pans?

    Quote:

    Inthepit said:
    Are people preferring a swab over an inoculation loop?
    So far the only advantage, I read about, is not introducing more water from LC or syringe. Otherwise a loop seems more straight forward.





    It's not that I have a preference I just litterally have never owned, made or tried  using a loop. Swabs have been readily available


    --------------------
    :amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

    Look around you... Everything you see exists inside the mind.
    Consciousness, the awareness that is experiencing this mind,
    is peering in from outside the universe.

    Our individual experiences are all part of the universe's experience of itself


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    OfflineGrimsweeper
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    Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid] * 1
        #26427691 - 01/11/20 08:24 AM (4 years, 1 month ago)

    :whathesaid:
    I've used swabs since I started and never saw a reason to switch. Sterile packs of 200 are pretty cheap.


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    InvisibleBph
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    Re: Let's All Grow Mushrooms 2020 [Re: Grimsweeper] * 1
        #26427740 - 01/11/20 09:09 AM (4 years, 1 month ago)

    I started with a loop and switched to swabs and will never go back. I like the fact the whole thing is sterile out of the pack (or jar). I like how it disperses the spores when you streak it. Loops suck tbh. It works sure but I find the swabs to save me from having one big clump of myc. I get small organized colonies right out of the gate


    Edited by Bph (01/11/20 12:27 PM)


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    InvisibleBph
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    Re: Let's All Grow Mushrooms 2020 [Re: A.k.a]
        #26427751 - 01/11/20 09:13 AM (4 years, 1 month ago)

    I started with a loop and switched to swabs and will never go back. I like the fact the whole thing is sterile out of the pack. I like how it disperses the spores when you streak it. Loops suck tbh. It works sure but I find the swabs to save me from having one big clump of myc. I get small organize colonies right out of the gate
    Quote:

    A.k.a said:
    Does nutrient content matter for spore plates or just softness?

    I know for spores 10 days isn’t that long but I have so many plates swiped i feel like something should’ve grown by now.





    Hang in there. My Lucy GT spores just started to do something from.12/7. I was about to throw it out.


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