|
thekickingbird


Registered: 11/22/19
Posts: 26
Last seen: 2 years, 11 months
|
Need your advice on SP to Agar
#26426351 - 01/10/20 11:22 AM (4 years, 19 days ago) |
|
|
Dear expert mycologists,
After waiting for 2 months I just get my GT and a Tamenensis SP order. Currently I live in Argentina and acquiring them were so difficult, plus the prints are small (less than 1x1 inch or 2x2cm).
So I need your advice to maximice the change of success. I was thinking on doing spore print to agar directly because I have found a lot of information in this forums saying that there is better because you can cut and "clean" contamination on agar. However there are much more tek's on agar to syringes or LC.
What do you think? SP to Agar or SP to Syringe? Are there other safer options?
Thanks a lot in advance!
|
WeavieWonder
Time Travel Sucks



Registered: 08/18/16
Posts: 1,455
Loc: USA
Last seen: 1 month, 3 days
|
|
SP >> agar for best results. Any other way and you are gambling.
|
BLINKfan420
Jedi



Registered: 07/06/12
Posts: 1,078
Last seen: 5 months, 16 hours
|
Re: Need your advice on SP to Agar [Re: WeavieWonder]
#26426508 - 01/10/20 01:10 PM (4 years, 19 days ago) |
|
|
Spore print > agar.
Hands down. There is no debate.
As soon as you get a shroom print that biotch.
BLiNK
--------------------
  All my pictures are drawn from imagination. I don't even know what a mushroom is.
Edited by BLINKfan420 (01/10/20 01:10 PM)
|
Drboomer
The lord magnificent



Registered: 09/22/19
Posts: 957
|
Re: Need your advice on SP to Agar [Re: BLINKfan420]
#26426539 - 01/10/20 01:19 PM (4 years, 19 days ago) |
|
|
Spore print to agar I've used the same tiny little print to do 10 plates.
|
Bph
Stranger



Registered: 10/11/18
Posts: 1,466
|
Re: Need your advice on SP to Agar [Re: Drboomer]
#26426567 - 01/10/20 01:27 PM (4 years, 19 days ago) |
|
|
Use a sterile swab. Either buy them pre sterilized from a medical supplier or put some q tips in a jar and PC it for 20 min. Touch the swab to the print then draw a W across your agar dish. No point to make a syringe unless your only going to do cakes.
|
thekickingbird


Registered: 11/22/19
Posts: 26
Last seen: 2 years, 11 months
|
Re: Need your advice on SP to Agar [Re: Bph]
#26427061 - 01/10/20 06:34 PM (4 years, 19 days ago) |
|
|
Thanks a lot for your advice guys!! I'll do SP > agar and I'll keep you updated!!
|
thekickingbird


Registered: 11/22/19
Posts: 26
Last seen: 2 years, 11 months
|
|
Update: 5 days later and this is how the GT petris look like:

The Tampanensis ones didn't show any signs of mycilium or contamination so far. I think the SP we're too light or thin and I should scrap harder next time. I also did 2 kind of inoculation, one with a homemade inoculation tool and the other with the scalpel. Both esterilized and flame esterilized between plates.
What do you think about this petri? It is my very first time so I'm eager for your advice.
Thanks!!!!!
|
Jooby
Stranger



Registered: 11/13/19
Posts: 174
Last seen: 2 years, 1 month
|
|
Quote:
thekickingbird said: Update: 5 days later and this is how the GT petris look like:

The Tampanensis ones didn't show any signs of mycilium or contamination so far. I think the SP we're too light or thin and I should scrap harder next time. I also did 2 kind of inoculation, one with a homemade inoculation tool and the other with the scalpel. Both esterilized and flame esterilized between plates.
What do you think about this petri? It is my very first time so I'm eager for your advice.
Thanks!!!!!
I'm new to agar as well. That said, I would def take some transfers from that now.
|
tramalot
Stranger


Registered: 12/13/19
Posts: 199
Loc: USA
|
|
Quote:
thekickingbird said: Update: 5 days later and this is how the GT petris look like:

The Tampanensis ones didn't show any signs of mycilium or contamination so far. I think the SP we're too light or thin and I should scrap harder next time. I also did 2 kind of inoculation, one with a homemade inoculation tool and the other with the scalpel. Both esterilized and flame esterilized between plates.
What do you think about this petri? It is my very first time so I'm eager for your advice.
Thanks!!!!!
I was told by somebody who has done this before it's best to take a small swab like mentioned above but then put it in a sterile shot glass and add a millilitre or 2 of sterile water and then swish the swab and then suck them up into the syringe and let them sit for 24 hours and hydrate a little bit and then just use one tiny drop on each plate.
I'm new and I'm still learning so take what I say with a grain of salt....
-------------------- It's the Journey I enjoy!
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 34 minutes
|
Re: Need your advice on SP to Agar [Re: tramalot]
#26438283 - 01/17/20 10:27 AM (4 years, 12 days ago) |
|
|
I think you got two methods mixed together unless it’s just one I haven’t heard yet.
Usually with the swab after stirring the drops in the shot glass you then swab the plate to spread spores on it.
Idk a ton about it though so totally possible there’s a reason so suck it back up then drip on the plate beside dilution of spores.
--------------------
LAGM2020     
Edited by A.k.a (01/17/20 10:29 AM)
|
thekickingbird


Registered: 11/22/19
Posts: 26
Last seen: 2 years, 11 months
|
Re: Need your advice on SP to Agar [Re: A.k.a]
#26438352 - 01/17/20 11:11 AM (4 years, 12 days ago) |
|
|
Thanks for your feedback!!!!! I'm kinda traumatized of syringes, before I get this spores I tried a syringe I won in a cubes workshop with no success.
But I'll give a try with the tiny Tampanensis SP, can you send me a link to that Tek you are talking about?
Thank a lot !!!
|
thekickingbird


Registered: 11/22/19
Posts: 26
Last seen: 2 years, 11 months
|
|
Guys I have an update on my agar, what do you think, is this mycilium healthy??
Thanks!
Edited by thekickingbird (01/20/20 09:35 PM)
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
Transfer time
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
thekickingbird


Registered: 11/22/19
Posts: 26
Last seen: 2 years, 11 months
|
Re: Need your advice on SP to Agar [Re: cronicr]
#26444296 - 01/21/20 05:21 AM (4 years, 8 days ago) |
|
|
Perfect, thanks a lot for your advice!
|
|