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rido



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What the hell did I isolate? "Pan cyan" LC to agar, black growth under mycelium
#26425629 - 01/09/20 11:20 PM (4 years, 19 days ago) |
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So I had a very light Pan cyan AU print on cardstock. I had a hard time getting spores off of it. I tried a moistened Q-tip to agar, tried flaking spores off with a razor, and ended up accidentally dropping the print itself into LC. This was in an attempt to scrape spores off with a razor.
A week or so later, growth! It sure looked like mycelium. I put it onto some grain in a "learn by doing" adventure and saw the growth to be light and wispy. This grain was prepared way too dry and the jar stalled, but I still had some of the original LC.
I finally got around to getting a PC and actual petri dishes, so I decided to try and clean up the LC. The first couple transfers brought bacteria, but these last few plates appear to be bacteria free.
These plates are MDA. All three of these plates were transfers from different colonies on the previous plate. I haven't seen any signs of bacterial contamination. Growth starts light and wispy, looks healthy, and then gets this darkening underneath itself.

The bottom left plate is a bottom view. The bottom view of the other two is similar.
Did I isolate something other than Pan cyan?
Edited by rido (01/09/20 11:29 PM)
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BLINKfan420
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Re: What the hell did I isolate? "Pan cyan" LC to agar, black growth under mycelium [Re: rido]
#26425653 - 01/09/20 11:35 PM (4 years, 19 days ago) |
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The top and bottom right plates look like Trichoderma to me. The bottom left looks like some other contamination. I've never grown Cyans but I'm under the impression that the mycelium does not look like that. Maybe whispier but white none-the-less.
BLiNK
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  All my pictures are drawn from imagination. I don't even know what a mushroom is.
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rido



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Re: What the hell did I isolate? "Pan cyan" LC to agar, black growth under mycelium [Re: BLINKfan420]
#26425684 - 01/10/20 12:02 AM (4 years, 19 days ago) |
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Quote:
BLINKfan420 said: The top and bottom right plates look like Trichoderma to me. The bottom left looks like some other contamination. I've never grown Cyans but I'm under the impression that the mycelium does not look like that. Maybe whispier but white none-the-less.
BLiNK
Thanks! I'm also under the impression that it shouldn't look like this. Luckily I have a separate Pan cyan Estero (Florida) print that was much darker. I just started it on agar a few days ago and it's got a few spots that have germinated already.
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jcm4620
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Re: What the hell did I isolate? "Pan cyan" LC to agar, black growth under mycelium [Re: rido]
#26426016 - 01/10/20 05:32 AM (4 years, 19 days ago) |
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ya that dont look good at all. im new to pans also and i still dont have the eye down on the mycelium yet. so here are a cpl pics maybe they will help ya. one is a pan cyan clone(tissue sample) on agar and 1 is of it after was put on grain. im sorry for the bad pic of the dish i know its hard to c with the cling wrap but i had ran out of para film at the time. anyways hope this helps ya a lil

Edited by jcm4620 (01/10/20 05:34 AM)
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rido



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Re: What the hell did I isolate? "Pan cyan" LC to agar, black growth under mycelium [Re: jcm4620]
#26426044 - 01/10/20 06:18 AM (4 years, 19 days ago) |
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That helps a ton actually! Having all three to look at - a cube plate, my three "pan" plates, and your actual pan plate makes it very clear. Into the trash they go. Oops!
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jcm4620
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Re: What the hell did I isolate? "Pan cyan" LC to agar, black growth under mycelium [Re: rido]
#26426278 - 01/10/20 10:15 AM (4 years, 19 days ago) |
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it happens man keep at it
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MLPismyOPSEC
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Re: What the hell did I isolate? "Pan cyan" LC to agar, black growth under mycelium [Re: rido] 1
#26426295 - 01/10/20 10:29 AM (4 years, 19 days ago) |
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Bottom left one looks like you're staring into T̵̠̣̝̠̃͐̐̈̅͗̚͘͜͝Ḩ̴̠̯̯̋E̴̘̮͓̖͙͍̻̥͊͊̀ ̵̢̬͚̳̠͇͙̰̝̪͚͔͉́͛̑͘͘̚͜͝V̴̛̯͇̒̿̈́̄̅́̂̍̓̓̈́̃̉̕͜O̶̧̧̼͉̩̗̺͔̙̊ͅḮ̴̡͕̭̙̘̠̫̹̼̑͜D̴͇̯͓͕̱͚̤͚̥̅̊̆̈́͗̂̽̒̕͘̚͠
These two threads should give you a good idea what to look for! https://www.shroomery.org/forums/showflat.php/Number/17231150/fpart/1/vc/1 https://www.shroomery.org/forums/showflat.php/Number/25987049/fpart/1
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rido



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Re: What the hell did I isolate? "Pan cyan" LC to agar, black growth under mycelium [Re: MLPismyOPSEC]
#26427106 - 01/10/20 06:54 PM (4 years, 19 days ago) |
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Quote:
MLPismyOPSEC said: Bottom left one looks like you're staring into T̵̠̣̝̠̃͐̐̈̅͗̚͘͜͝Ḩ̴̠̯̯̋E̴̘̮͓̖͙͍̻̥͊͊̀ ̵̢̬͚̳̠͇͙̰̝̪͚͔͉́͛̑͘͘̚͜͝V̴̛̯͇̒̿̈́̄̅́̂̍̓̓̈́̃̉̕͜O̶̧̧̼͉̩̗̺͔̙̊ͅḮ̴̡͕̭̙̘̠̫̹̼̑͜D̴͇̯͓͕̱͚̤͚̥̅̊̆̈́͗̂̽̒̕͘̚͠
These two threads should give you a good idea what to look for! https://www.shroomery.org/forums/showflat.php/Number/17231150/fpart/1/vc/1 https://www.shroomery.org/forums/showflat.php/Number/25987049/fpart/1
Thank you for those links. I've seen those threads before, but having something in front of me to compare to makes it so much more obvious.
New plate of Pan cyan is off to a good start I think. I know the red-circled colonies are bacterial, and the yellow circled colonies look nearly identical to how one of the plates above started. That leads me to believe the blue circled colonies are Pan cyan. Thoughts?
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MLPismyOPSEC
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Re: What the hell did I isolate? "Pan cyan" LC to agar, black growth under mycelium [Re: rido]
#26427150 - 01/10/20 07:21 PM (4 years, 18 days ago) |
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Always good to have reference material! The blue circle looks promising, i'm not as familiar with pan myc as i am cube myc, but it also doesn't look like mold just yet.
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rido



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Re: What the hell did I isolate? "Pan cyan" LC to agar, black growth under mycelium [Re: MLPismyOPSEC]
#26427198 - 01/10/20 07:56 PM (4 years, 18 days ago) |
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Do you agree that the yellow circle does look like mold, then? They are very feint but there are three in there.
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Near Dylan
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Re: What the hell did I isolate? "Pan cyan" LC to agar, black growth under mycelium [Re: rido]
#26427203 - 01/10/20 08:01 PM (4 years, 18 days ago) |
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Ive never seen a grow screw up so bad that it opens a black hole
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rido



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Re: What the hell did I isolate? "Pan cyan" LC to agar, black growth under mycelium [Re: Near Dylan]
#26427409 - 01/10/20 11:19 PM (4 years, 18 days ago) |
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Quote:
Near Dylan said: Ive never seen a grow screw up so bad that it opens a black hole
That makes two of us!
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MLPismyOPSEC
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Re: What the hell did I isolate? "Pan cyan" LC to agar, black growth under mycelium [Re: rido]
#26427642 - 01/11/20 07:13 AM (4 years, 18 days ago) |
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Yeah, i think the yellow circle is mold. You'll know if it starts to overtake the whole plate within a couple days.
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rido



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Re: What the hell did I isolate? "Pan cyan" LC to agar, black growth under mycelium [Re: MLPismyOPSEC]
#26428364 - 01/11/20 03:30 PM (4 years, 18 days ago) |
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I'm a bit less sure about this now.
 The blue-circled colonies (two total) look like there's some discoloration forming. There are a total of six of the yellow circled colonies; yellow squares are the location of colonies that aren't visible in the image.
All of the growth is on the swab-path so I'm not really sure about any of it. Thoughts?
Edit: I did some more searching/comparing and I'm a bit more confident again that the two blue-circled colonies are Pan cyan.
Edited by rido (01/12/20 01:05 AM)
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rido



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Re: What the hell did I isolate? "Pan cyan" LC to agar, black growth under mycelium [Re: rido]
#26430098 - 01/12/20 05:57 PM (4 years, 17 days ago) |
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Okay I have a very similar contam on a plate of cube spores (same as the yellow circled colonies above) so I'm going to go ahead with my assumption that the blue circled colonies are legitimately Pan cyan.
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