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InvisibleJohnW
Trip Advisor

Registered: 12/14/19
Posts: 32
Transferring In Vitro Pin To Agar?
    #26423119 - 01/08/20 02:57 PM (4 years, 21 days ago)

So I've got this little baby mush growing in one of my cake jars. I'm assuming that's what everyone is referring to as a pin. It's the first mush I've ever grown. :cool:

I'm wondering if when I birth the jar I can take that little guy and throw it on agar? Would it convert back to myc? Should I cut it in half or something?



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OfflineBLINKfan420
Jedi
I'm a teapot


Registered: 07/06/12
Posts: 1,078
Last seen: 5 months, 16 hours
Re: Transferring In Vitro Pin To Agar? [Re: JohnW]
    #26423125 - 01/08/20 03:01 PM (4 years, 21 days ago)

You could cut that pin into several pieces and put it to agar and get some mycelium growing, yes.

BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


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InvisibleJohnW
Trip Advisor

Registered: 12/14/19
Posts: 32
Re: Transferring In Vitro Pin To Agar? [Re: BLINKfan420]
    #26423128 - 01/08/20 03:03 PM (4 years, 21 days ago)

Ok Cool! Thx!


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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Transferring In Vitro Pin To Agar? [Re: JohnW]
    #26423169 - 01/08/20 03:27 PM (4 years, 21 days ago)

You don't have to cut it. You could just throw the whole thing on agar. It will grow. For best results, clean the jar with iso and do it in a SAB (this may be obvious, just making sure)


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InvisibleJohnW
Trip Advisor

Registered: 12/14/19
Posts: 32
Re: Transferring In Vitro Pin To Agar? [Re: verum subsequentis]
    #26423181 - 01/08/20 03:34 PM (4 years, 21 days ago)

Yes, being sterile is the plan. I'm assuming it's still sterile in the jar. Thanks for making sure! I think I'll prob end up doing two plates with it, just to be safe.

That jar has just finished colonizing so I'm going to give it another week.


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Offlineverum subsequentis
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Re: Transferring In Vitro Pin To Agar? [Re: JohnW]
    #26423192 - 01/08/20 03:45 PM (4 years, 21 days ago)

Nice


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OfflineBLINKfan420
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I'm a teapot


Registered: 07/06/12
Posts: 1,078
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Re: Transferring In Vitro Pin To Agar? [Re: verum subsequentis]
    #26423250 - 01/08/20 04:19 PM (4 years, 21 days ago)

I would agree that noc'ing up at least two plates is a smart move.  Contaminations will find their way in and the plates you have going the better your chances are.

BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


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InvisibleJohnW
Trip Advisor

Registered: 12/14/19
Posts: 32
Re: Transferring In Vitro Pin To Agar? [Re: BLINKfan420]
    #26423284 - 01/08/20 04:50 PM (4 years, 21 days ago)

Yeah, that's what I'm thinking too.  Not a bad idea to have a backup.

Man, I love that pic of the mush on the cake at the bottom of your posts.


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OfflineA.k.aM
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Re: Transferring In Vitro Pin To Agar? [Re: JohnW]
    #26423398 - 01/08/20 06:10 PM (4 years, 21 days ago)

lol I just sent a message earlier asking about that pic.

What kind is it???

The cap shape/pattern on the big ones is perfect.


--------------------
LAGM2020


Edited by A.k.a (01/08/20 06:11 PM)


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InvisibleJohnW
Trip Advisor

Registered: 12/14/19
Posts: 32
Re: Transferring In Vitro Pin To Agar? [Re: A.k.a]
    #26423436 - 01/08/20 06:49 PM (4 years, 21 days ago)

Quote:

A.k.a said:
lol I just sent a message earlier asking about that pic.

What kind is it???

The cap shape/pattern on the big ones is perfect.




Haha! It looks like the mush on the Petty video "Don't Come Around Here No More"


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OfflineBLINKfan420
Jedi
I'm a teapot


Registered: 07/06/12
Posts: 1,078
Last seen: 5 months, 16 hours
Re: Transferring In Vitro Pin To Agar? [Re: JohnW]
    #26423725 - 01/08/20 10:22 PM (4 years, 21 days ago)

Golden Teachers from multispore. Way back in like 2010 when I first got into this stuff.

:rockon:

BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


Edited by BLINKfan420 (01/08/20 10:22 PM)


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