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DoctorDoggo
Experienced
Registered: 01/08/20
Posts: 6
Last seen: 3 years, 5 months
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Humans - I am a dog, need halp, agar first time
#26422993 - 01/08/20 01:41 PM (4 years, 21 days ago) |
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Hello humans,
I was going to put this in the contamination forum, however not all questions are not contamination related so forgive me if I made a mistake.
I am a golden retriever. I am what you would call a microbiologist (if you were to extend that classification beyond your own species) who is growing some fungus in malt extract agar. My friend, a mixed breed dog, dropped off a spore print of what I was told was P. cubensis B+.
After sealing up my jars in my SAB I had to leave for a very important business trip (dog business, nothing of human concern), but the caretaker of my jars reported no growth up until precisely three weeks.
The first sign of growth were the two very large colonies that you see below. Several days later the smaller colonies popped up and I instructed my colleague to place the jar inside a ziplock to store inside the nearest human refrigerator until my return.
It is winter here and indoor temperatures are lower than ideal, between 65-68 degrees Fahrenheit.
My questions to you humans are: what is the yellowish mucous color that you see on the two large colonies, and what do you think is the large circular mycelium looking growth on one of the two large colonies?
My first thought is that the yellowish color is not a bacterial or fungal contaminate, partly because my control jar (no spores) is clean and my aseptic technique is very good. However, before I transfer those colonies to a different agar jar I'd like to know your thoughts. My feeling is the two larger colonies are simply overgrown.
Additionally, do you think I should take samples of the larger colonies or the smaller colonies? I'm trying to select only the best colonies; what phenotypic criteria do you look for when selecting, if any?
Pictures:
TOP:



Bottom:

Side (two large colonies)

I apologize for the poor quality. I am a dog. It is hard to use a camera.
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JohnW
Trip Advisor

Registered: 12/14/19
Posts: 32
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Re: Humans - I am a dog, need halp, agar first time [Re: DoctorDoggo]
#26423040 - 01/08/20 02:10 PM (4 years, 21 days ago) |
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I'm a newb so take what I say with a grain of salt. I've heard a little about myc piss. And also have a little yellow on a couple of my agar plates. It might be normal.
It is my understanding that you should pick the most vigorous growth.
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Kmacmo
The aborted pin



Registered: 08/14/19
Posts: 1,675
Loc: Central hemisphere
Last seen: 7 hours, 34 minutes
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Re: Humans - I am a dog, need halp, agar first time [Re: DoctorDoggo]
#26423057 - 01/08/20 02:19 PM (4 years, 21 days ago) |
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You dirty animal, shouldn't be near agar or mycelium. I love dogs but why don't you go take a shit or chase a cat or something.
A humans refrigerator can be very dirty best to use a mini-fridge dedicated to your mush stuff. The two big yellow bits do look like mycelium with some kinda bacteria/contam growing on top of them.
I would take lots of transfers from the small ones (steering clear of the yellow stuff) since its a germination plate you ideally just want to take transfers as soon as you see growth giving contams less chance to infect the entire plate. Pictures are not the best so hard to tell properly but you should search and read about mycelium agar sectoring and rhizmorphic growth... Once you know what your looking for you you'll be able to look at your plates/jars and know what bits look good and such.
If the large colonies did not have that yellow on them I would target them for transfers.
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footpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 2 months
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Re: Humans - I am a dog, need halp, agar first time [Re: Kmacmo]
#26423065 - 01/08/20 02:24 PM (4 years, 21 days ago) |
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feldman114
Stragler


Registered: 09/06/19
Posts: 3,365
Loc: Bravos
Last seen: 3 years, 9 months
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Re: Humans - I am a dog, need halp, agar first time [Re: footpath]
#26423072 - 01/08/20 02:28 PM (4 years, 21 days ago) |
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Those are contams imo. Looks like bacteria growing on myc.
Doesn’t matter how your control jar is doing cause you knocked up with spores.
I’d transfer from the small ones.
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DoctorDoggo
Experienced
Registered: 01/08/20
Posts: 6
Last seen: 3 years, 5 months
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Re: Humans - I am a dog, need halp, agar first time [Re: feldman114]
#26423124 - 01/08/20 03:00 PM (4 years, 21 days ago) |
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Thank you, humans. You are very helpful!
Much love.
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