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OfflineFetteredFaux
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Registered: 11/11/19
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Contaminated petri dishes?
    #26421860 - 01/07/20 07:49 PM (4 years, 22 days ago)

Heya!
First time posting on here but I've been lurking around for quite awhile. I've been pretty successful with BFR cakes so far, but I wanted to try and work with some agar to get a feel for it, but I'm not too sure if it went well.



I used BOD's Comprehensive Agar Tek
specifically his BFR agar mix so there's some sediment in there from it, but I was wondering, is this mycelium? and if so, is this safe to inoculate a grain jar with?

Thanks!


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Offlinecli_hlt
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Re: Contaminated petri dishes? [Re: FetteredFaux]
    #26424058 - 01/09/20 07:18 AM (4 years, 20 days ago)

That looks like healthy mycelium to me. The top-right one might be contaminated with bacteria (spots on the furface). The others looks perfectly fine. :greatjob:


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Invisiblewildernessjunkie
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Re: Contaminated petri dishes? [Re: cli_hlt]
    #26431853 - 01/13/20 05:44 PM (4 years, 16 days ago)

The one on the top right is no good. I would do some transfers with the rest.


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OfflineFetteredFaux
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Re: Contaminated petri dishes? [Re: wildernessjunkie]
    #26432926 - 01/14/20 10:15 AM (4 years, 15 days ago)

Just inoculated my first grain jars with some of the cleaner looking agar.

Thanks for the replies and insight!


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OfflinePinconning1
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Re: Contaminated petri dishes? [Re: FetteredFaux]
    #26434660 - 01/15/20 10:45 AM (4 years, 14 days ago)

This question just seems kind of redundant. Why do you have petri dishes if your asking if they are contaminated. What are you even doing with them?


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Invisiblewildernessjunkie
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Re: Contaminated petri dishes? [Re: Pinconning1]
    #26434976 - 01/15/20 01:34 PM (4 years, 14 days ago)

Quote:

Pinconning1 said:
This question just seems kind of redundant. Why do you have petri dishes if your asking if they are contaminated. What are you even doing with them?






Just like everything else, agar has a bit of a learning curve. It takes some practice to learn to identify what looks good and what looks bad. Plates frequently are contaminated, and they are supposed to do that. So that we can isolate the desired organism away from the undesired. Its when we stop seeing those undesireables, that we know that we are good to go.


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