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trubblesome
Stranger



Registered: 11/09/19
Posts: 406
Last seen: 11 months, 4 days
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Re: Let's All Grow Mushrooms 2020 [Re: MooseMomma] 1
#26421218 - 01/07/20 02:22 PM (4 years, 1 month ago) |
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I'd also suggest acquiring or making your own loop. put a squirt of that spore syringe in a clean shot glass or an unused sterile petri dish, then dip a (sterilized by flame and then cooled) loop in it and draw an S/Z pattern with it across the plate so that the spores and the bacteria can get more separation.
re: soot, ime soot is the product of incomplete combustion from whatever fuel your heat source uses. soot = needs more air. a properly dialed in burner/torch/whatever won't leave soot. A fisher burner is the tool I miss the most from the lab, but I'm not quite at the point where I'm gonna pipe gas to my mush cult space.
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SFS96
AstroMan



Registered: 12/09/18
Posts: 2,144
Loc: Valleys Of Neptune
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Re: Let's All Grow Mushrooms 2020 [Re: trubblesome]
#26421231 - 01/07/20 02:30 PM (4 years, 1 month ago) |
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Most of my plates are fucked. Looks like I got a bad batch of syringes. Only the ape swab I used has decent growth. I think I’m going to put some different spores to plates in a bit.
-------------------- How I make and preserve tea
Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood
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MooseMomma
Crazy Old Pitbull Lady


Registered: 12/20/19
Posts: 18
Loc: Coastal Carolina
Last seen: 2 years, 8 months
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Re: Let's All Grow Mushrooms 2020 [Re: SFS96]
#26421355 - 01/07/20 03:26 PM (4 years, 1 month ago) |
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I’m in the same boat, I only had spores in syringes and they were both contaminated. Won’t use that vendor again. Someone else on the forum thread took pity on me and is sending me spores. I feel blessed and appreciate the Shroomery community so mush! DM me.
-------------------- The people who say it cannot be done should stay out of the way of those who are doing it.
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Thufir_Hawat
Mentat


Registered: 03/18/17
Posts: 399
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Re: Let's All Grow Mushrooms 2020 [Re: MooseMomma] 1
#26421363 - 01/07/20 03:30 PM (4 years, 1 month ago) |
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Finally got some action on one plate out of 6, the LGT! Clearly follows my swab pattern and since I am not seeing anything else growing on any of the spore plates I'm feeling good about my technique and pours at least. Really hoping the PE and PF:RS take off eventually.
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 4 months
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Re: Let's All Grow Mushrooms 2020 [Re: Thufir_Hawat] 1
#26421381 - 01/07/20 03:41 PM (4 years, 1 month ago) |
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Short Update:
January 7th, 2020.
Pleasantly surprised with the vigor in this PR. I wrote the previous notes for Jan 6th because I hadn't gone to sleep yet so for me it was still the 6th, but it was more like 6am on the 7th. My PR plates already has visible myc growth coming from the transfer wedge less than 12 hours after transfer. Doesn't show up that well on my camera, so I will skip the photo for now.
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
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Re: Let's All Grow Mushrooms 2020 [Re: MooseMomma] 1
#26421382 - 01/07/20 03:44 PM (4 years, 1 month ago) |
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Quote:
MooseMomma said: I’m in the same boat, I only had spores in syringes and they were both contaminated. Won’t use that vendor again. Someone else on the forum thread took pity on me and is sending me spores. I feel blessed and appreciate the Shroomery community so mush! DM me.
Did you use agar??
You should be able to clean up contaminated spore syringes, unless they are really fucked maybe it would be difficult for a beginner but, good learning experience nonetheless.
If you haven't, I recommend starting to use agar in your grow procedure. It will make life much easier and reduce your contamination. Also you will never run out of spores. With agar a spore syringe could last forever.
So I have some bigger spots, Definitely germination confirmed and I am just going to take some transfers since the growth is really faint. The growth seems super light, I was experimenting with new agar recipe I am gonna transfer a couple wedges to the old recipe just to see if there is much difference. I haven't grown this variety out yet.
 
Soon I will update my main post more with all the information (make it into a grow log)
Also I don't think I said it but hats off to CBK for this thread...really awesome for the community and fun to participate. Also giving me some motivation to work a little quicker .
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
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Re: Let's All Grow Mushrooms 2020 [Re: sh4d0ws]
#26421384 - 01/07/20 03:46 PM (4 years, 1 month ago) |
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Sorry moosemama
I missed your agar posts. Maybe tooo much weed 
What I started doing recently was squirting spores onto a sterile swab and swabbing the plate. It's been working good for me. And the swab absorbs the excess water so it actually works quite well. I just made sure to get a good drop of spores on there and swabbed away.
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MooseMomma
Crazy Old Pitbull Lady


Registered: 12/20/19
Posts: 18
Loc: Coastal Carolina
Last seen: 2 years, 8 months
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Re: Let's All Grow Mushrooms 2020 [Re: sh4d0ws]
#26421405 - 01/07/20 04:01 PM (4 years, 1 month ago) |
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Yes, my syringes were completely contaminated and the contaminated solution with the spores in it start growing really quick so the spores in that solution don’t have a chance to germinate. I did really well with spore syringe solution to agar beck in December with a spore syringe from a different vendor. My P Cyan on agar is growing like gang busters. I used the agar to inoculate a grain jar and 3 more plates, all are growing great. I am going to use the grain jar with a hardwood sawdust substrate in an outdoor bed designed to replicate the forest floor with oak mulch and hardwood sawdust and then cased with moss.
-------------------- The people who say it cannot be done should stay out of the way of those who are doing it.
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Tattersail



Registered: 04/11/18
Posts: 285
Last seen: 2 years, 1 day
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Re: Let's All Grow Mushrooms 2020 [Re: sh4d0ws] 1
#26421414 - 01/07/20 04:06 PM (4 years, 1 month ago) |
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Im seeing a teeny poof of white fuzz in the TOC plate, yay! Hopefully there will be something to transfer at the weekend. If it's not mould, that is.
I noticed a lot of soot on my DIY innoc loops too, though it may have been because I used a candle to flame with.
-------------------- LAGM2021 Trades We may lose or we may win, but we'll never be here again
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footpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 3 months
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Re: Let's All Grow Mushrooms 2020 [Re: Tattersail] 2
#26421627 - 01/07/20 06:04 PM (4 years, 1 month ago) |
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Let's All Grow Mushrooms 2020 [Re: footpath]
#26421628 - 01/07/20 06:07 PM (4 years, 1 month ago) |
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Standard berznzomatic for me no alc or soaking of the blade and I use an exacto as my scalp all.
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TeaforTwo
Flight Attendant




Registered: 09/22/19
Posts: 1,005
Loc: Psychonaut City, Mile Hi!
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Re: Let's All Grow Mushrooms 2020 [Re: eatyualive]
#26421644 - 01/07/20 06:17 PM (4 years, 1 month ago) |
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thats tight footpath, movin it- cool vid and that music, it pairs well
gotta get pics off camera, thought it'd be simpler than the sms/email game. nothing really exciting, some growth and looks good so far, will transfer in few days. in other news, we're printing and have cloned two purple mystic fruits that weighed in at 50g and 39g respectively, and respectfully! came from brf cakes shred to 6q shoebox on 7Dec, first anything out of it.
-------------------- See a mush cult pic you love? add it here: January 2020 PoM nominations thread! (up vote your faves to ensure they are included in final vote!). Community selected winner receives a 6mo Supporter account upgrade and/or other prizes from our awesome admin team! Looking for something? Try Psilosopherr's Tek Compendium and pixelpopper's essentialzzz ForTheWin! Be well, friend. Stay Strong and Beautiful, Always, and in All Ways~ Look Forward, Seek Truth, Find Real, Go Further~
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,776
Loc: A thought
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Re: Let's All Grow Mushrooms 2020 [Re: eatyualive] 1
#26421645 - 01/07/20 06:18 PM (4 years, 1 month ago) |
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We should all make guesses as to the total dry weight of active fungus this event adds to the earth by the first day of 2021!
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 4 months
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Re: Let's All Grow Mushrooms 2020 [Re: Smartattack]
#26421647 - 01/07/20 06:20 PM (4 years, 1 month ago) |
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Think I finally got this video thing figured out. Not nearly as good as footpath though.
Edited by Randalf the Grey (01/07/20 06:34 PM)
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footpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 3 months
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Re: Let's All Grow Mushrooms 2020 [Re: TeaforTwo] 1
#26421649 - 01/07/20 06:21 PM (4 years, 1 month ago) |
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Yeah, something about agar transfers just shouts bossa nova waiting room to me.
edit: Not showing up for me, Randalf. Looks like you might need to just copy the video link from vimeo and put that in the embed tool.
Edited by footpath (01/07/20 06:28 PM)
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TheWitchfinder

Registered: 08/28/13
Posts: 76
Loc: Salem
Last seen: 3 years, 5 months
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Re: Let's All Grow Mushrooms 2020 [Re: footpath]
#26421652 - 01/07/20 06:23 PM (4 years, 1 month ago) |
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Your videos and documentation are awesome, footpath. Keep it up.
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 4 months
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Re: Let's All Grow Mushrooms 2020 [Re: footpath] 1
#26421655 - 01/07/20 06:23 PM (4 years, 1 month ago) |
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I usually listen to Michel Petrucciani or some other smooth jazz while doing SAB work. Recently fell in love with esbjorn svensson trio and rymden.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,386
Loc: where?
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YouTube blocked the music from one of my sab vids
Maybe dio wasnt a good choice
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,776
Loc: A thought
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Re: Let's All Grow Mushrooms 2020 [Re: mushboy]
#26421671 - 01/07/20 06:29 PM (4 years, 1 month ago) |
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Dio is always the best choice.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Let's All Grow Mushrooms 2020 [Re: footpath] 1
#26421673 - 01/07/20 06:29 PM (4 years, 1 month ago) |
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JAN 7 Noticing some germination on the second plate now. Made my first transfer from the first plate.
 Only took one transfer. Kept the germ plates just in case something goes wrong but should be fine
Also put 3 drops of KSSS spore solution to agar and swished it around a bit.
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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