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InvisibleRRedBBeard
SE Connecticut
Male
Registered: 05/28/12
Posts: 385
Loc: SE CT
Does this chestnut LC look ok?
    #26402513 - 12/27/19 05:36 AM (4 years, 1 month ago)

I got a commercially-produced chestnut mush grow kit a week ago, and (hopefully!) pulled sterile cells from the bag before opening it. A blank, sterile LC jar was injected with this, and almost nothing was immediately visible in the new jar. This pic shows explosive, 'fluffy' growth after 6 days, and I'll noc an agar jar with it today. Are the dark 'granules' that developed in these a cause for concern?
Thanks.




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InvisibleLotLizard
Stranger

Registered: 10/21/18
Posts: 92
Re: Does this chestnut LC look ok? [Re: RRedBBeard]
    #26402964 - 12/27/19 12:18 PM (4 years, 1 month ago)

Could be rust from your lids if you aren’t using plastic. Hard to tell off of the picture and information. Agar is really the only way to tell if it’s good or not.


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InvisibleRRedBBeard
SE Connecticut
Male
Registered: 05/28/12
Posts: 385
Loc: SE CT
Re: Does this chestnut LC look ok? [Re: LotLizard]
    #26403063 - 12/27/19 01:55 PM (4 years, 1 month ago)

I added a drop of unsulfured molasses to the recipe, otherwise the cultures tend to be clear.


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InvisibleRRedBBeard
SE Connecticut
Male
Registered: 05/28/12
Posts: 385
Loc: SE CT
Re: Does this chestnut LC look ok? [Re: RRedBBeard]
    #26411809 - 01/02/20 02:53 AM (4 years, 1 month ago)

This chestnut LC is encouraging. I made a syringe from it, and the myc continues to reproduce, the liquid is still crystal clear in both cultures. The agar jar test isn't doing anything yet, but I'm going to make a supplemented sawdust bag and see what happens....


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InvisibleRRedBBeard
SE Connecticut
Male
Registered: 05/28/12
Posts: 385
Loc: SE CT
Re: Does this chestnut LC look ok? [Re: RRedBBeard]
    #26420399 - 01/07/20 05:17 AM (4 years, 1 month ago)

The agar/pholiota adiposa sprang to life, and seems well colonized after 10 days (pictured). I just PC'd a grow bag with ~5 pounds of amended sawdust, so we'll see how the p.adiposa LC does with that. I made 7 brf jars at the same time, and want to see what this wood-lover does in that medium. If the jar looks good, then I'll use it to noc another, larger sawdust bag.The pic is as clear as I can get it.
I'll keep you all posted!



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