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ZenZone



Registered: 02/18/17
Posts: 931
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Re: Portugal Hunting & Brown spots on cap [Re: stickp1]
#26423298 - 01/08/20 05:00 PM (4 years, 21 days ago) |
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Quote:
stickp1 said: Sorry mr. Zone newb here, don't know what you mean...
By "feeding" them I meant that maybe you could spread some wood chips, woody debris, finely chopped wood in the are where they grow, so that they could thrive and by next season they would fruit in greater numbers
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Doc9151
Mycologist



Registered: 02/23/17
Posts: 13,753
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Re: Portugal Hunting & Brown spots on cap [Re: ZenZone]
#26423464 - 01/08/20 07:09 PM (4 years, 21 days ago) |
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PM sent
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  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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stickp1
Stranger

Registered: 01/06/20
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Re: Portugal Hunting & Brown spots on cap [Re: ZenZone]
#26423942 - 01/09/20 03:49 AM (4 years, 20 days ago) |
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I see now, thanks for clearing that up. I will definitely consider it!
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Anglerfish
hearing things



Registered: 09/08/10
Posts: 18,643
Loc: Norvegr
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Re: Portugal Hunting & Brown spots on cap [Re: stickp1]
#26423962 - 01/09/20 04:18 AM (4 years, 20 days ago) |
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You should be able to get these sequenced in your neighbouring country at alvalab.es. I believe you need to request a DNA extraction plus an ITS sequence. It will probably cost you around 20 € plus postage.
Alan Rockefeller should however be able to give you the correct instructions for these procedures. Wondering why he hasn't chimed in on this thread. Maybe you should change the post title and include "possible P. azurescens" or something like that?
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stickp1
Stranger

Registered: 01/06/20
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Re: Portugal Hunting & Brown spots on cap [Re: Anglerfish]
#26425026 - 01/09/20 04:30 PM (4 years, 20 days ago) |
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I pmed mr. Rockefeller.
Meanwhile caught a few more small caramels today, perhaps the rhizomorphs help to clarify what the species is. Also one of the fellows seemed like he was being used as substrate for something else...
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Anglerfish
hearing things



Registered: 09/08/10
Posts: 18,643
Loc: Norvegr
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Re: Portugal Hunting & Brown spots on cap [Re: stickp1]
#26426232 - 01/10/20 09:31 AM (4 years, 19 days ago) |
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Such ropey rhizomorphic mycelium is apparently characteristic of several of the woodloving species of Psilocybe.
That second picture looks really like P. azurescens though.
I really hope we get to the bottom of what this actually is.
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adfhweaga
Stranger
Registered: 07/18/19
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Re: Portugal Hunting & Brown spots on cap [Re: Anglerfish]
#26426336 - 01/10/20 11:11 AM (4 years, 19 days ago) |
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Quote:
Anglerfish said: You should be able to get these sequenced in your neighbouring country at alvalab.es. I believe you need to request a DNA extraction plus an ITS sequence. It will probably cost you around 20 € plus postage.
Alan Rockefeller should however be able to give you the correct instructions for these procedures. Wondering why he hasn't chimed in on this thread. Maybe you should change the post title and include "possible P. azurescens" or something like that?
Wait, what. You can have any biological material sequenced for 20 EUR?!
I feel like I just traveled to the future! I had no idea. Super interesting.
Also I often go to Lisbon. I'm totally going to "visit Sintra". PM me if anybody wants to join
Edited by adfhweaga (01/10/20 11:11 AM)
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Anglerfish
hearing things



Registered: 09/08/10
Posts: 18,643
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Re: Portugal Hunting & Brown spots on cap [Re: adfhweaga]
#26427533 - 01/11/20 04:17 AM (4 years, 18 days ago) |
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Quote:
adfhweaga said: Wait, what. You can have any biological material sequenced for 20 EUR?!
Just an estimate on the cost of extraction and a basic ITS sequence. More thorough analysis would obviously cost more. I never sent them anything myself, I got the prices from an associate.
It's all here, though:
http://www.alvalab.es/orders.html
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stickp1
Stranger
Registered: 01/06/20
Posts: 17
Loc: Portugal
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Re: Portugal Hunting & Brown spots on cap [Re: Anglerfish]
#26448519 - 01/23/20 04:12 PM (4 years, 6 days ago) |
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Hello again fellas. Update on consumption.
Dried a few of these batches in the sun with a black t-shirt over them until cracker-dry after which stored them in a tupperware in fridge for a few days. See pictures below.
1st time:
Very low dose to check how I would react. ~0.5g was chopped with a knife and placed on steaming kettle water (w/ a bit of lemon juice) where it was steeped for 5-10 min. Only drank the water. T+1h Felt the body heavy and yawned a lot, had a little trouble concentrating on what I was doing. Heart rate seemed irregular for up to T+5h. Otherwise no effect. Bear in mind I have never tried Psilocybe before.
2nd time (2 days later):
~1g was chopped up. Placed in pan filled with water (+ bit lemon juice) on low heat and stirred intermittently until water was almost boiling (bubbles on the pan's surface), which took ~15 min. Felt pretty much the same as before with heavy body but almost unnoticeable. T+3h After going outside to smoke a cig I notice my eyelids feel heavy and overall my body feels slightly strange, mind is clear. T+4h Walking becomes strange like I have some problem with my legs (can't stop from swinging my hip when taking a step), but just walking normally, when jumping or running it disappears. I notice also that I can't blink my eyes properly, and the motor ability of my arms and hands seems affected, specially keeping arm/hand/finger raised for a few seconds, it started shaking and losing strength, a bit like if had fallen asleep on it.
I had heard of woodlover's paralysis, so I didn't worry a lot. In any case it was pretty disappointing to only experience what I had no interest in experiencing, and I will abstain from repeating such an experience. If anyone has any solid insight on how preparation can mitigate these effects while increasing the psychoactive compounds' concentration, or how in other parts of the process (picking, drying...) one can avoid this shit paralysis I would appreciate it. Or anything I may be doing wrong for that matter.
For now this little mofo will take a hiatus from experimenting with his bod...
  
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Anglerfish
hearing things



Registered: 09/08/10
Posts: 18,643
Loc: Norvegr
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Re: Portugal Hunting & Brown spots on cap [Re: stickp1]
#26448635 - 01/23/20 05:19 PM (4 years, 6 days ago) |
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Sounds weird. If these mushrooms are what we believe, you should at least get some kind of psychedelic effect. Maybe you're just immune in some way, apparently it happens to some people - given that you never have taken psilocybin previously. I'm kind of sensitive to it, and a half gram of liberty caps - that apparently are weaker than your assumed P. azurescens - will definitely make me trippy if not properly tripping.
Do you still have some dried material? Any chance you could send some to Alvalab for sequencing?
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stickp1
Stranger
Registered: 01/06/20
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Loc: Portugal
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Re: Portugal Hunting & Brown spots on cap [Re: Anglerfish]
#26449707 - 01/24/20 10:20 AM (4 years, 5 days ago) |
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Yes I still have some, and they are still appearing at one location. Yes I will do some research and perhaps try to get the sequencing done... Thanks for the feedback!
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Anglerfish
hearing things



Registered: 09/08/10
Posts: 18,643
Loc: Norvegr
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Re: Portugal Hunting & Brown spots on cap [Re: stickp1]
#26449734 - 01/24/20 10:34 AM (4 years, 5 days ago) |
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You're not taking any kind of medication regularly, are you?
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Shroomhunts
Hunter Gatherer



Registered: 05/07/18
Posts: 2,928
Loc: PA
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Re: Portugal Hunting & Brown spots on cap [Re: Anglerfish]
#26449909 - 01/24/20 12:25 PM (4 years, 5 days ago) |
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I have a buddy who can go to Walmart on 7g, some people are just naturally resistant to them
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      You never kno
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jonnyvanbatman
Portugal

Registered: 02/15/20
Posts: 2
Loc: Portugal
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Re: Portugal Hunting & Brown spots on cap [Re: Shroomhunts]
#26486685 - 02/15/20 08:24 AM (3 years, 11 months ago) |
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iNaturalist Observations
Hey! I have been going to Sintra to identify mushrooms and will be start living there for my internship. I have been studying these supposed azurescens and am currently talking to someone who will analyze them and confirm a species that can be still not confirmed in Portugal. I added a link where i put all my observations and there are 4 or 5 from this species in the last months. Alan Rockefeller identified them as azurescens but in the older observations on Mushroom Observer that were made there is a discussion on wether these are azurescens or bohemica, and there are pictures of microscopy done by the guy who found them! If any of you know the microscopic features I would love to know some opinions, and I have a lot of photos on my observations that can help.
Glad there are other people interested and looking forward to learn more about psilocybes in Portugal!
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Doc9151
Mycologist



Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
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Re: Portugal Hunting & Brown spots on cap [Re: jonnyvanbatman]
#26486772 - 02/15/20 09:38 AM (3 years, 11 months ago) |
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Quote:
jonnyvanbatman said: iNaturalist Observations
Hey! I have been going to Sintra to identify mushrooms and will be start living there for my internship. I have been studying these supposed azurescens and am currently talking to someone who will analyze them and confirm a species that can be still not confirmed in Portugal. I added a link where i put all my observations and there are 4 or 5 from this species in the last months. Alan Rockefeller identified them as azurescens but in the older observations on Mushroom Observer that were made there is a discussion on wether these are azurescens or bohemica, and there are pictures of microscopy done by the guy who found them! If any of you know the microscopic features I would love to know some opinions, and I have a lot of photos on my observations that can help.
Glad there are other people interested and looking forward to learn more about psilocybes in Portugal!
if this person doesn't come through, I will be willing to pay for the ITS sequencing to get an answer. PM me if it doesn't work out, it's worth the $15-$20 in my opinion to see what they are and expand the known distribution ranges.
--------------------
  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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stickp1
Stranger
Registered: 01/06/20
Posts: 17
Loc: Portugal
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Re: Portugal Hunting & Brown spots on cap [Re: jonnyvanbatman]
#26487041 - 02/15/20 12:46 PM (3 years, 11 months ago) |
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Hey there jonny, very good to hear from you! From what I see, we have been going to very similar places just a few days apart ahah Funny enough I am also waiting for confirmation on the exact species, as I don’t have the tools myself. I will share as soon as I am able. Feel free to pm me, I feel there is plenty to discuss!
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adfhweaga
Stranger
Registered: 07/18/19
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Re: Portugal Hunting & Brown spots on cap [Re: Doc9151]
#26558980 - 03/26/20 11:23 AM (3 years, 9 months ago) |
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Quote:
Doc9151 said:
Quote:
jonnyvanbatman said: iNaturalist Observations
Hey! I have been going to Sintra to identify mushrooms and will be start living there for my internship. I have been studying these supposed azurescens and am currently talking to someone who will analyze them and confirm a species that can be still not confirmed in Portugal. I added a link where i put all my observations and there are 4 or 5 from this species in the last months. Alan Rockefeller identified them as azurescens but in the older observations on Mushroom Observer that were made there is a discussion on wether these are azurescens or bohemica, and there are pictures of microscopy done by the guy who found them! If any of you know the microscopic features I would love to know some opinions, and I have a lot of photos on my observations that can help.
Glad there are other people interested and looking forward to learn more about psilocybes in Portugal!
if this person doesn't come through, I will be willing to pay for the ITS sequencing to get an answer. PM me if it doesn't work out, it's worth the $15-$20 in my opinion to see what they are and expand the known distribution ranges.
I'll put in 10 EUR on top of that.
It would be super cool that the shroom grows in Portugal. What Portugal season would it grow in best?
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SarahP
beginner

Registered: 11/06/20
Posts: 22
Loc: Portugal
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Re: Portugal Hunting & Brown spots on cap [Re: stickp1]
#27355622 - 06/19/21 08:04 PM (2 years, 7 months ago) |
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Hi, I live near Sintra.
Any news about this?
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Lalalaaz
Stranger

Registered: 12/04/21
Posts: 1
Last seen: 1 year, 9 months
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Re: Portugal Hunting & Brown spots on cap [Re: stickp1]
#27568928 - 12/04/21 10:58 AM (2 years, 1 month ago) |
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Found this in Sintra today…
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Doc9151
Mycologist


Registered: 02/23/17
Posts: 13,753
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Re: Portugal Hunting & Brown spots on cap [Re: Lalalaaz] 1
#27571411 - 12/06/21 01:31 PM (2 years, 1 month ago) |
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That looks like a weathered Psilocybe azurescens.
--------------------
  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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