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Arkansashunter
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24/24 jars failed help a rookie?
#26419717 - 01/06/20 05:32 PM (4 years, 23 days ago) |
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Hey guys,
Thanks in advance for any help.
I recently got into attempting cultivation and have done lots of research and felt like it was going well. Had good growth starting even though it was a little slow.
24 / 24 jars turned bad with what I believe (was told by community here) was Bacillus. Awful smell in the jars smearing when shaking etc.
I have some agar plates that I did at the same time and have no noticable contamination.
I'm trying to figure out where I went wrong. I did a whole lot of jars expecting to lose a few but not 100% failure.
I am using oats and pressure cooked them for 2 hours at 15psi.
I am using a SAB and I sterilized the box after every few transfers just in case. I flamed sterilized between each transfer but that means going in and out of my box with my arms. Could this be my weak spot?
My jars were 3/4 full or maybe a little more. Am I failing due to poor GE? I have 2 holes in the lid with micropore tape over the holes. But I was wondering if my jars being too full limited the GE. Some jars were a little moist after PC could this be the culprit?
I probably have 6 agar plates with no obvious contaminants 3 of which are starting to pin in the container after 5 weeks.
Does anybody have any ideas where I'm dropping the ball before I waste these genetics?
One more question. Should I dump my whole agar plate into my jars? Should I remove the pin before dropping it in?
Sorry for the rambling mess. I'm just disheartened and looking for the holes in my game.
Thanks again
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Tormato  
The Goddess Kali Meh 😛




Registered: 07/01/17
Posts: 6,067
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Using micropore tape as your GE filter is where you went wrong ....use polyfil or SFD in the future.
Dropping agar into jars I usually cut the agar into 6 wedges...1 wedge per jar...it's really up to you.
I remove the pins before dropping
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Diego


Registered: 12/30/19
Posts: 346
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Re: 24/24 jars failed help a rookie? [Re: Tormato]
#26419754 - 01/06/20 05:58 PM (4 years, 23 days ago) |
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Ive never used oats but 2 hours seems like a long time
-------------------- Some do it for the income But we do it for the outcome Some of us are active While others just let their mouths run - Chali 2na & Jurassic 5
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Tormato  
The Goddess Kali Meh 😛




Registered: 07/01/17
Posts: 6,067
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Re: 24/24 jars failed help a rookie? [Re: Diego]
#26419756 - 01/06/20 05:59 PM (4 years, 23 days ago) |
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Quote:
Diego said: Ive never used oats but 2 hours seems like a long time
90-120 mins is the norm @ 15-18psi
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Diego


Registered: 12/30/19
Posts: 346
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Re: 24/24 jars failed help a rookie? [Re: Tormato]
#26419785 - 01/06/20 06:20 PM (4 years, 23 days ago) |
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Is this standard for all grains or just oats in particular?
-------------------- Some do it for the income But we do it for the outcome Some of us are active While others just let their mouths run - Chali 2na & Jurassic 5
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Tormato  
The Goddess Kali Meh 😛




Registered: 07/01/17
Posts: 6,067
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Re: 24/24 jars failed help a rookie? [Re: Diego] 1
#26419792 - 01/06/20 06:26 PM (4 years, 23 days ago) |
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Quote:
Diego said: Is this standard for all grains or just oats in particular?
All grains
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Diego


Registered: 12/30/19
Posts: 346
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Re: 24/24 jars failed help a rookie? [Re: Tormato]
#26419797 - 01/06/20 06:28 PM (4 years, 23 days ago) |
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Thanks dude
-------------------- Some do it for the income But we do it for the outcome Some of us are active While others just let their mouths run - Chali 2na & Jurassic 5
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Tormato  
The Goddess Kali Meh 😛




Registered: 07/01/17
Posts: 6,067
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Re: 24/24 jars failed help a rookie? [Re: Diego]
#26419805 - 01/06/20 06:37 PM (4 years, 23 days ago) |
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No prob!
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Arkansashunter
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Re: 24/24 jars failed help a rookie? [Re: Tormato]
#26420218 - 01/06/20 11:59 PM (4 years, 22 days ago) |
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Tormato I hope I tagged you correctly thanks for the reply.
I have seen a Tek using the post office bags as a filter I would guess is technically a SFB. Is this sufficient or should I order something more official.
I'm not questioning your knowledge but I'm curious how confident you are that the poly tape was my issue? It would definitely explain the 100% failure. I just want to stop questioning myself. I dont want to lose the genetics. Hope that was respectful enough. Thanks so much for your time Tormato.
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JohnRainy
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Polyfill works like a hot damn.
 
Synthetic filter disks are very convenient and durable.
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cronicr



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Re: 24/24 jars failed help a rookie? [Re: JohnRainy]
#26420247 - 01/07/20 12:52 AM (4 years, 22 days ago) |
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Micropore tape works for a lot of people but needs to be at least 2 layers. So just to clarify you inoculated all jars with wedges?
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Tormato  
The Goddess Kali Meh 😛




Registered: 07/01/17
Posts: 6,067
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26420308 - 01/07/20 02:49 AM (4 years, 22 days ago) |
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Quote:
Arkansashunter said: Tormato I hope I tagged you correctly thanks for the reply.
I have seen a Tek using the post office bags as a filter I would guess is technically a SFB. Is this sufficient or should I order something more official.
I'm not questioning your knowledge but I'm curious how confident you are that the poly tape was my issue? It would definitely explain the 100% failure. I just want to stop questioning myself. I dont want to lose the genetics. Hope that was respectful enough. Thanks so much for your time Tormato.
If your drilling holes in the lids and just covering them with 1 layer of micropore tape that's going to be a failure point IMO. Even with 2 layers I still don't trust it like I do with polyfil or SFD....I'm old sorry 
Quote:
cronicr said: So just to clarify you inoculated all jars with wedges?
That was me Whole plate or I'll cut wedges...just depends what I'm working with
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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cronicr



Registered: 08/07/11
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Re: 24/24 jars failed help a rookie? [Re: Tormato]
#26420310 - 01/07/20 02:54 AM (4 years, 22 days ago) |
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He never actually said he used agar for the jars , just that he also had some going
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26420335 - 01/07/20 03:45 AM (4 years, 22 days ago) |
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My poly fill jars with a silicon injection port on oats and some results from oats in the background.
I had rye go bad like that. Smelled like sour mash.
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Arkansashunter
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Re: 24/24 jars failed help a rookie? [Re: YogiBear] 1
#26420730 - 01/07/20 10:25 AM (4 years, 22 days ago) |
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Thanks for everyones time.
I did use agar in the jars. I cut them up with a flame sterilized scalpel and transferred a couple pieces in each jar.
The growth was going well in most jars but then turned for the worst.
I will pick up some poly fill tomorrow when I make it into the city.
I have been using micropore tape on the holes in my agar plates too. Should I bail on that as well?
Last menial question I have is tyvek an adequate material for SFD? Or should I wait for polyfill?
Thanks again Tormato and Cronicr
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cronicr



Registered: 08/07/11
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No I use micropore on my agar jars it doesn't let contams through . I would ditch the oats
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Diego


Registered: 12/30/19
Posts: 346
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26420847 - 01/07/20 11:32 AM (4 years, 22 days ago) |
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Quote:
Arkansashunter said: Thanks for everyones time.
I did use agar in the jars. I cut them up with a flame sterilized scalpel and transferred a couple pieces in each jar.
The growth was going well in most jars but then turned for the worst.
I will pick up some poly fill tomorrow when I make it into the city.
I have been using micropore tape on the holes in my agar plates too. Should I bail on that as well?
Last menial question I have is tyvek an adequate material for SFD? Or should I wait for polyfill?
Thanks again Tormato and Cronicr
IQuote:
cronicr said: No I use micropore on my agar jars it doesn't let contams through . I would ditch the oats
Does micropore tape work better than parafilm? It sure as hell is cheaper
-------------------- Some do it for the income But we do it for the outcome Some of us are active While others just let their mouths run - Chali 2na & Jurassic 5
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: 24/24 jars failed help a rookie? [Re: Diego]
#26420853 - 01/07/20 11:36 AM (4 years, 22 days ago) |
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No! It's a sticky mess on plates lol
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Diego


Registered: 12/30/19
Posts: 346
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26420886 - 01/07/20 11:50 AM (4 years, 22 days ago) |
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Quote:
cronicr said: No! It's a sticky mess on plates lol
Ok. You said jar. My bad dude. Shit i thought I found a corner to cut! Haha
-------------------- Some do it for the income But we do it for the outcome Some of us are active While others just let their mouths run - Chali 2na & Jurassic 5
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A.k.a
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Re: 24/24 jars failed help a rookie? [Re: Diego]
#26420897 - 01/07/20 11:56 AM (4 years, 22 days ago) |
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Use the tyvek. When I was doing syringe to wbs jars I used tyvek with silicone on top then mp tape over it after injection just to be safe.
The only time a jar was a total loss I didn’t let the ship dry enough and when I took the jar out of the pc the silicon was stuck to the foil and pulled the tyvek off with it when I unwrapped it.
I just slapped micropore tape over the hole instead of putting tyvek back. It made it to the shake and then never recovered.
It still blows my mind how many oat jars go bacterial. Wbs forever for me.
--------------------
LAGM2020     
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Dartho
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Re: 24/24 jars failed help a rookie? [Re: A.k.a]
#26421119 - 01/07/20 01:28 PM (4 years, 22 days ago) |
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Although I am just gearing up for my first grow, and have no working knowledge: I think we need to hear more about your grain prep. I'm thinking perhaps the moisture level wasn't where you need it one way or another, I'm thinking too wet.
At least that's more info we can get to draw conclusions.
-------------------- Dartho - Chaotic good 'Sith lord' elf from Elfwood, near Dreamland. Will force lightning tickle you until you pee your pants.
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Zachsonpub
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Quote:
Arkansashunter said: I am using a SAB and I sterilized the box after every few transfers just in case.
You'll read this time and time again, but you aren't trying to sanitize the box, you're just trying to keep the air still (Still Air box)... So don't wipe anything down between transfers unless you knock the box off your surface or something extreme. If that does happen, wipe shit down, let the air settle like you did at the start.
You've got it right on you agar plates it appears, so it may be the lids as mentioned before.
Quote:
One more question. Should I dump my whole agar plate into my jars? Should I remove the pin before dropping it in?
I dump it all, I don't care about pins. It should all be sterile, or close to it. I often have a plate per jar or 2 that I'll slice up, dump in the receiving grains and it's colonized in about a week.
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FreeCanadianHugs
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I think like the poster above me. SAB boxes are not sterile as much as weighted down the contaminates. Yes clean your SAB before use, but most importantly spray inside the SAB so the mist falls down and as it falls bonds with fine air particles and brings them to the floor of your SAB. This allows for a much cleaner working area at higher elevation in your SAB. When you bring things in and out of your SAB spray as you just created air currents that will disturb the contaminates and bring them up to a higher level in the SAB again.
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FreeCanadianHugs
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50th post
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SpunkyMonkey88
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Re: 24/24 jars failed help a rookie? [Re: A.k.a]
#26421157 - 01/07/20 01:50 PM (4 years, 22 days ago) |
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Quote:
A.k.a said: Use the tyvek. When I was doing syringe to wbs jars I used tyvek with silicone on top then mp tape over it after injection just to be safe.
The only time a jar was a total loss I didn’t let the ship dry enough and when I took the jar out of the pc the silicon was stuck to the foil and pulled the tyvek off with it when I unwrapped it.
I just slapped micropore tape over the hole instead of putting tyvek back. It made it to the shake and then never recovered.
It still blows my mind how many oat jars go bacterial. Wbs forever for me.
What do you mean? You're saying there's a trend on here with oats and bacterial jars?
you got me here bro.
I really don't think the type of grain could ever dictate contamination rates.
If you PC your grains properly they should be 100% sterile.
Edited by SpunkyMonkey88 (01/07/20 01:50 PM)
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cronicr



Registered: 08/07/11
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No pats are horrible for bacteria, some grains contain more endospores than others
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Arkansashunter
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26421172 - 01/07/20 02:01 PM (4 years, 22 days ago) |
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I was feeling so dang confident after doing so well with the Agar I just couldnt figure out where I went wrong.
I'm very greatful for all of the response. I'll try to find something else to use as spawn my next visit to town.
I'm way out in the sticks as you could imagine oats were available.
Does anyone have any recommendations besides WBS. Nothing against WBS just incase i cant find a good source.
Thanks again everyone
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cronicr



Registered: 08/07/11
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Wheat rye millet
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Zachsonpub
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26421190 - 01/07/20 02:08 PM (4 years, 22 days ago) |
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If you're in the sticks, chances are you have feeds stores around. I get millet at mine. So easy to work with. Oats easy af, too.
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HappyHigh
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Re: 24/24 jars failed help a rookie? [Re: Zachsonpub]
#26421250 - 01/07/20 02:39 PM (4 years, 22 days ago) |
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I would say you need to work on technique. I use no SAB just my kitchen, gloves, a spore print, and a butter knife. scrape off spores whisk in oats (24/hr after PC) use a lid with single layer 3m micrpore and even sometimes no lid ..tinfoil... no contams. i don't even use a breathing mask i do turn off all airflow, fans, a/c.
-------------------- Had I the heavens' embroidered cloths, Enwrought with golden and silver light, The blue and the dim and the dark cloths Of night and light and the half light, I would spread the cloths under your feet: But I, being poor, have only my dreams; I have spread my dreams under your feet; Tread softly because you tread on my dreams.
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SpunkyMonkey88
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26421267 - 01/07/20 02:45 PM (4 years, 22 days ago) |
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Quote:
cronicr said: No pats are horrible for bacteria, some grains contain more endospores than others
Was this in response to my comment where i stated type of grain shouldn't play a role in contamination?
I get that one grain can have more (endo)spores then the next... but shouldn't a proper PC cycle kill them anyways?
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cronicr



Registered: 08/07/11
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In theory it should but often it doesn't
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Diego


Registered: 12/30/19
Posts: 346
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Quote:
SpunkyMonkey88 said:
Quote:
cronicr said: No pats are horrible for bacteria, some grains contain more endospores than others
Was this in response to my comment where i stated type of grain shouldn't play a role in contamination?
I get that one grain can have more (endo)spores then the next... but shouldn't a proper PC cycle kill them anyways?
Usually soaking them long enough will cause them to germinate so the pc will kill them
-------------------- Some do it for the income But we do it for the outcome Some of us are active While others just let their mouths run - Chali 2na & Jurassic 5
Edited by Diego (01/07/20 02:50 PM)
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cronicr



Registered: 08/07/11
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Re: 24/24 jars failed help a rookie? [Re: Diego]
#26421286 - 01/07/20 02:56 PM (4 years, 22 days ago) |
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No hydrating the grain is all you need, longer soak times can result in more endospores than you started with. Once an endospores germinated it's no longer an endospores it's just bacteria, soaking softens everything making it easier to eradicate
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Diego


Registered: 12/30/19
Posts: 346
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26421300 - 01/07/20 03:00 PM (4 years, 22 days ago) |
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Quote:
cronicr said: No hydrating the grain is all you need, longer soak times can result in more endospores than you started with. Once an endospores germinated it's no longer an endospores it's just bacteria, soaking softens everything making it easier to eradicate
I soak 12 hrs then pc. Whats your method?
-------------------- Some do it for the income But we do it for the outcome Some of us are active While others just let their mouths run - Chali 2na & Jurassic 5
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: 24/24 jars failed help a rookie? [Re: Diego]
#26421303 - 01/07/20 03:02 PM (4 years, 22 days ago) |
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For oats? Hydrate and pc, they stopped carrying them here though so I'm using millet or wheat
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Diego


Registered: 12/30/19
Posts: 346
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26421313 - 01/07/20 03:06 PM (4 years, 22 days ago) |
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I buy wbs by the ton for my turkey so its what i use. Is it not the same method?
-------------------- Some do it for the income But we do it for the outcome Some of us are active While others just let their mouths run - Chali 2na & Jurassic 5
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Zachsonpub
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26421314 - 01/07/20 03:06 PM (4 years, 22 days ago) |
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I was actually pissed with millet using a tek where you just add dry millet, water and a lil gypsum to jar then pc. My only prob was that I couldn't safely fit 10 in my presto as 3 would be on their side and would leak that water.
Recently was reading a book and they mentioned just putting a lid under the jar at the lid to prop them up during PC cycle. Something so obvious...
Anyway, sorry for the
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SpunkyMonkey88
Stranger



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Re: 24/24 jars failed help a rookie? [Re: Zachsonpub]
#26421328 - 01/07/20 03:15 PM (4 years, 22 days ago) |
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Quote:
Zachsonpub said: I was actually pissed with millet using a tek where you just add dry millet, water and a lil gypsum to jar then pc. My only prob was that I couldn't safely fit 10 in my presto as 3 would be on their side and would leak that water.
Recently was reading a book and they mentioned just putting a lid under the jar at the lid to prop them up during PC cycle. Something so obvious...
Anyway, sorry for the 
Ha I dont let stuff like that bother me, I'm happy to fit 7 jars in my presto .. that's pretty dam good IMO...
Plus I dont like the idea of having jars on their sides... I'll just stick to the 7...
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Diego


Registered: 12/30/19
Posts: 346
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Quote:
SpunkyMonkey88 said:
Quote:
Zachsonpub said: I was actually pissed with millet using a tek where you just add dry millet, water and a lil gypsum to jar then pc. My only prob was that I couldn't safely fit 10 in my presto as 3 would be on their side and would leak that water.
Recently was reading a book and they mentioned just putting a lid under the jar at the lid to prop them up during PC cycle. Something so obvious...
Anyway, sorry for the 
Ha I dont let stuff like that bother me, I'm happy to fit 7 jars in my presto .. that's pretty dam good IMO...
Plus I dont like the idea of having jars on their sides... I'll just stick to the 7...
Cant you get a divider and put some pints up top? I have the same pc. Never done it but it looks like there's room
-------------------- Some do it for the income But we do it for the outcome Some of us are active While others just let their mouths run - Chali 2na & Jurassic 5
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StygianKnight
A Mushroom

Registered: 03/12/12
Posts: 2,717
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Re: 24/24 jars failed help a rookie? [Re: Zachsonpub]
#26421337 - 01/07/20 03:19 PM (4 years, 22 days ago) |
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If all the jars went bad with the exact same thing and only that thing, then there’s a good chance there’s a reservoir of it somewhere that you are transferring to them. While bacteria can certainly exist in the air, it’s more of a contact transfer from one item to another or through your own breathing and sneezing, etc traveling on microdroplets. So it’s probably on something (potentially even the agar), and getting transferred into the jars.
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SpunkyMonkey88
Stranger



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Re: 24/24 jars failed help a rookie? [Re: Diego]
#26421344 - 01/07/20 03:21 PM (4 years, 22 days ago) |
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Quote:
Diego said:
Quote:
SpunkyMonkey88 said:
Quote:
Zachsonpub said: I was actually pissed with millet using a tek where you just add dry millet, water and a lil gypsum to jar then pc. My only prob was that I couldn't safely fit 10 in my presto as 3 would be on their side and would leak that water.
Recently was reading a book and they mentioned just putting a lid under the jar at the lid to prop them up during PC cycle. Something so obvious...
Anyway, sorry for the 
Ha I dont let stuff like that bother me, I'm happy to fit 7 jars in my presto .. that's pretty dam good IMO...
Plus I dont like the idea of having jars on their sides... I'll just stick to the 7...
Cant you get a divider and put some pints up top? I have the same pc. Never done it but it looks like there's room
Do you mean stack some pints upright ontop of the quarts? If so then no I don't think so. Maybe a layer of 1/2 pints ontop but that would be it...
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Diego


Registered: 12/30/19
Posts: 346
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Quote:
SpunkyMonkey88 said:
Quote:
Diego said:
Quote:
SpunkyMonkey88 said:
Quote:
Zachsonpub said: I was actually pissed with millet using a tek where you just add dry millet, water and a lil gypsum to jar then pc. My only prob was that I couldn't safely fit 10 in my presto as 3 would be on their side and would leak that water.
Recently was reading a book and they mentioned just putting a lid under the jar at the lid to prop them up during PC cycle. Something so obvious...
Anyway, sorry for the 
Ha I dont let stuff like that bother me, I'm happy to fit 7 jars in my presto .. that's pretty dam good IMO...
Plus I dont like the idea of having jars on their sides... I'll just stick to the 7...
Cant you get a divider and put some pints up top? I have the same pc. Never done it but it looks like there's room
Do you mean stack some pints upright ontop of the quarts? If so then no I don't think so. Maybe a layer of 1/2 pints ontop but that would be it...
Idk. Im at work. Might be worth a check
-------------------- Some do it for the income But we do it for the outcome Some of us are active While others just let their mouths run - Chali 2na & Jurassic 5
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Zachsonpub
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Re: 24/24 jars failed help a rookie? [Re: Diego]
#26421561 - 01/07/20 05:29 PM (4 years, 22 days ago) |
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Quote:
Diego said:
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SpunkyMonkey88 said:
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Diego said:
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SpunkyMonkey88 said:
Ha I dont let stuff like that bother me, I'm happy to fit 7 jars in my presto .. that's pretty dam good IMO...
Plus I dont like the idea of having jars on their sides... I'll just stick to the 7...
Cant you get a divider and put some pints up top? I have the same pc. Never done it but it looks like there's room
Do you mean stack some pints upright ontop of the quarts? If so then no I don't think so. Maybe a layer of 1/2 pints ontop but that would be it...
Idk. Im at work. Might be worth a check
Probably could, but I just like to have that extra 3 qts fit so if I'm trying to PC 30 jars, it's just 3 runs instead of 5. After heat up, venting, cook, then cool down, for me that's about 2.5hrs x 2.
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SpunkyMonkey88
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Registered: 10/08/19
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Re: 24/24 jars failed help a rookie? [Re: Zachsonpub]
#26421607 - 01/07/20 05:53 PM (4 years, 22 days ago) |
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Ya I guess in that instance itd be logical to cram it full but I've never had to PC more than 14 at a time anyways ...
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Arkansashunter
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I used Psilicon Oat Spawn Preparation Tek Pretty much to a T but now looking back he is using a filter disc not pore tape. Boil grains until hydrated strain grains pressure cook 2 hours at 15psi Then the next day I took them to SAB to transfer.
I also should mention I did this in 3 separate sessions. I did 8 then after my agar transfers I did 10 more then 6 more.
My agar is just Potato dex agar 500ml water 5 grams potato 9 grams agar. Most of my Agar dishes tuned out really well unless of course its growing the bacteria that im transferring. That being said I have 6 agar plates that are 5 weeks old with no sign of contamination from the same days I transferred to jars.
For the record I did have a 2 layers of micropore tape but I feel like it was likely my failure point after hearing the advice.
Im going to start some new Jars today I just went down and bought some fresh clean jars to cut out any doubts about the previously contaminated jars.
Im going to try Tyvek over my holes this time and Im going to transfer to more agar as insurance.
Also Im going to fill my jars just over half way this time. Not really worried about production I just need to get some success under my belt.
The Tyvek just seemed like it wouldnt exchange air well. I was probably wrong in assuming more air GE was better.
You guys make up such a great community thanks again for the responses.
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Diego


Registered: 12/30/19
Posts: 346
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You should of seen bacteria on your plates if it were there
-------------------- Some do it for the income But we do it for the outcome Some of us are active While others just let their mouths run - Chali 2na & Jurassic 5
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Arkansashunter
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Re: 24/24 jars failed help a rookie? [Re: Diego]
#26422207 - 01/07/20 11:47 PM (4 years, 21 days ago) |
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Thanks everyone
Figured I would show I'm not a complete dummy. This is a current agar plate of mine. This is from a cube I picked locally.
I simply tapped the cap onto an agar plate to get some spores and I also took a small section of the stem and then just transferred away until no contamination. Each time I would transfer I would make 3-5 plates per plate just to be safe. Mostly just for security. I can easily pick more next summer but that's a long way away.
I started trying to isolate some before all my jars contaminated and I got discouraged.
I said thanks alot already but you guys picked me up and got me motivated again so thank you all.

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LogicaL Chaos
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Thats pretty unusual to have a 100% fail rate. Hmmmmm....i would guess the grains werent processed/PC'd right or your agar was infected. Odd stuff.
I find its easier to work with small batches when trying something new. Then when you have confidence in your process, u can up the volume without having a total failure.
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YogiBear



Registered: 08/24/19
Posts: 845
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Quote:
LogicaL Chaos said: Thats pretty unusual to have a 100% fail rate. Hmmmmm....i would guess the grains werent processed/PC'd right or your agar was infected. Odd stuff.
I find its easier to work with small batches when trying something new. Then when you have confidence in your process, u can up the volume without having a total failure.

I've lost big batches too... TOTALLY sucks.
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TIS87
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Re: 24/24 jars failed help a rookie? [Re: YogiBear]
#26464584 - 02/02/20 03:00 AM (3 years, 11 months ago) |
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Did you purge the air in the PC?
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FrankRhizo
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Re: 24/24 jars failed help a rookie? [Re: TIS87] 1
#26464827 - 02/02/20 08:25 AM (3 years, 11 months ago) |
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Try and do a control jar next batch. If you swear it's not your cultures then it's in the grain prep or your sterile technique.
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