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InvisibleStygianKnight
A Mushroom

Registered: 03/12/12
Posts: 2,717
Trusted Cultivator
Re: 24/24 jars failed help a rookie? [Re: Zachsonpub]
    #26421337 - 01/07/20 03:19 PM (4 years, 22 days ago)

If all the jars went bad with the exact same thing and only that thing, then there’s a good chance there’s a reservoir of it somewhere that you are transferring to them.
While bacteria can certainly exist in the air, it’s more of a contact transfer from one item to another or through your own breathing and sneezing, etc traveling on microdroplets.  So it’s probably on something (potentially even the agar), and getting transferred into the jars.


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InvisibleSpunkyMonkey88
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Registered: 10/08/19
Posts: 1,331
Re: 24/24 jars failed help a rookie? [Re: Diego]
    #26421344 - 01/07/20 03:21 PM (4 years, 22 days ago)

Quote:

Diego said:
Quote:

SpunkyMonkey88 said:
Quote:

Zachsonpub said:
I was actually pissed with millet using a tek where you just add dry millet, water and a lil gypsum to jar then pc. My only prob was that I couldn't safely fit 10 in my presto as 3 would be on their side and would leak that water.

Recently was reading a book and they mentioned just putting a lid under the jar at the lid to prop them up during PC cycle. Something so obvious...

Anyway, sorry for the  :derail:




Ha I dont let stuff like that bother me, I'm happy to fit 7 jars in my presto
.. that's pretty dam good IMO...

Plus I dont like the idea of having jars on their sides... I'll just stick to the 7...



Cant you get a divider and put some pints up top? I have the same pc. Never done it but it looks like there's room




Do you mean stack some pints upright ontop of the quarts? If so then no I don't think so. Maybe a layer of 1/2 pints ontop but that would be it...


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InvisibleDiego


Registered: 12/30/19
Posts: 346
Re: 24/24 jars failed help a rookie? [Re: SpunkyMonkey88]
    #26421388 - 01/07/20 03:48 PM (4 years, 22 days ago)

Quote:

SpunkyMonkey88 said:
Quote:

Diego said:
Quote:

SpunkyMonkey88 said:
Quote:

Zachsonpub said:
I was actually pissed with millet using a tek where you just add dry millet, water and a lil gypsum to jar then pc. My only prob was that I couldn't safely fit 10 in my presto as 3 would be on their side and would leak that water.

Recently was reading a book and they mentioned just putting a lid under the jar at the lid to prop them up during PC cycle. Something so obvious...

Anyway, sorry for the  :derail:




Ha I dont let stuff like that bother me, I'm happy to fit 7 jars in my presto
.. that's pretty dam good IMO...

Plus I dont like the idea of having jars on their sides... I'll just stick to the 7...



Cant you get a divider and put some pints up top? I have the same pc. Never done it but it looks like there's room




Do you mean stack some pints upright ontop of the quarts? If so then no I don't think so. Maybe a layer of 1/2 pints ontop but that would be it...



Idk. Im at work. Might be worth a check


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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OfflineZachsonpub
Friend
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Registered: 03/02/16
Posts: 299
Last seen: 8 months, 20 days
Re: 24/24 jars failed help a rookie? [Re: Diego]
    #26421561 - 01/07/20 05:29 PM (4 years, 22 days ago)

Quote:

Diego said:
Quote:

SpunkyMonkey88 said:
Quote:

Diego said:
Quote:

SpunkyMonkey88 said:

Ha I dont let stuff like that bother me, I'm happy to fit 7 jars in my presto
.. that's pretty dam good IMO...

Plus I dont like the idea of having jars on their sides... I'll just stick to the 7...



Cant you get a divider and put some pints up top? I have the same pc. Never done it but it looks like there's room




Do you mean stack some pints upright ontop of the quarts? If so then no I don't think so. Maybe a layer of 1/2 pints ontop but that would be it...



Idk. Im at work. Might be worth a check




Probably could, but I just like to have that extra 3 qts fit so if I'm trying to PC 30 jars, it's just 3 runs instead of 5. After heat up, venting, cook, then cool down, for me that's about 2.5hrs x 2.


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InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: 24/24 jars failed help a rookie? [Re: Zachsonpub]
    #26421607 - 01/07/20 05:53 PM (4 years, 22 days ago)

Ya I guess in that instance itd be logical to cram it full but I've never had to PC more than 14 at a time anyways ...


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OfflineArkansashunter
Stranger
Registered: 07/15/19
Posts: 56
Last seen: 3 years, 9 months
Re: 24/24 jars failed help a rookie? [Re: SpunkyMonkey88]
    #26421695 - 01/07/20 06:40 PM (4 years, 22 days ago)

I used Psilicon Oat Spawn Preparation Tek
Pretty much to a T but now looking back he is using a filter disc not pore tape.
Boil grains until hydrated
strain grains
pressure cook 2 hours at 15psi
Then the next day I took them to SAB to transfer.

I also should mention I did this in 3 separate sessions. I did 8 then after my agar transfers I did 10 more then 6 more. 

My agar is just Potato dex agar 500ml water 5 grams potato 9 grams agar.
Most of my Agar dishes tuned out really well unless of course its growing the bacteria that im transferring.
That being said I have 6 agar plates that are 5 weeks old with no sign of contamination from the same days I transferred to jars.

For the record I did have a 2 layers of micropore tape but I feel like it was likely my failure point after hearing the advice.

Im going to start some new Jars today I just went down and bought some fresh clean jars to cut out any doubts about the previously contaminated jars.

Im going to try Tyvek over my holes this time and Im going to transfer to more agar as insurance.

Also Im going to fill my jars just over half way this time.
Not really worried about production I just need to get some success under my belt.

The Tyvek just seemed like it wouldnt exchange air well. I was probably wrong in assuming more air GE was better.

You guys make up such a great community thanks again for the responses.


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InvisibleDiego


Registered: 12/30/19
Posts: 346
Re: 24/24 jars failed help a rookie? [Re: Arkansashunter] * 1
    #26421711 - 01/07/20 06:48 PM (4 years, 22 days ago)

You should of seen bacteria on your plates if it were there


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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OfflineArkansashunter
Stranger
Registered: 07/15/19
Posts: 56
Last seen: 3 years, 9 months
Re: 24/24 jars failed help a rookie? [Re: Diego]
    #26422207 - 01/07/20 11:47 PM (4 years, 21 days ago)

Thanks everyone

Figured I would show I'm not a complete dummy. This is a current agar plate of mine. This is from a cube I picked locally.

I simply tapped the cap onto an agar plate to get some spores and I also took a small section of the stem and then just transferred away until no contamination.
Each time I would transfer I would make 3-5 plates per plate just to be safe. Mostly just for security. I can easily pick more next summer but that's a long way away.

I started trying to isolate some before all my jars contaminated and I got discouraged.

I said thanks alot already but you guys picked me up and got me motivated again so thank you all.





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OnlineLogicaL ChaosM
Ascension Energy & Alien UFOs
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Folding@home Statistics
Registered: 05/12/07
Posts: 69,328
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Last seen: 1 minute, 22 seconds
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Re: 24/24 jars failed help a rookie? [Re: Arkansashunter]
    #26422348 - 01/08/20 04:20 AM (4 years, 21 days ago)

Thats pretty unusual to have a 100% fail rate. Hmmmmm....i would guess the grains werent processed/PC'd right or your agar was infected. Odd stuff.

I find its easier to work with small batches when trying something new. Then when you have confidence in your process, u can up the volume without having a total failure.


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InvisibleYogiBear
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Registered: 08/24/19
Posts: 845
Re: 24/24 jars failed help a rookie? [Re: LogicaL Chaos]
    #26422387 - 01/08/20 05:45 AM (4 years, 21 days ago)

Quote:

LogicaL Chaos said:
Thats pretty unusual to have a 100% fail rate. Hmmmmm....i would guess the grains werent processed/PC'd right or your agar was infected. Odd stuff.

I find its easier to work with small batches when trying something new. Then when you have confidence in your process, u can up the volume without having a total failure.




:whathesaid:

I've lost big batches too... TOTALLY sucks.


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OfflineTIS87
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Registered: 11/22/19
Posts: 123
Last seen: 10 months, 1 day
Re: 24/24 jars failed help a rookie? [Re: YogiBear]
    #26464584 - 02/02/20 03:00 AM (3 years, 11 months ago)

Did you purge the air in the PC?


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OfflineFrankRhizo
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Registered: 11/28/17
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Re: 24/24 jars failed help a rookie? [Re: TIS87] * 1
    #26464827 - 02/02/20 08:25 AM (3 years, 11 months ago)

Try and do a control jar next batch. If you swear it's not your cultures then it's in the grain prep or your sterile technique.


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