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Dartho
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Registered: 01/02/20
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Re: 24/24 jars failed help a rookie? [Re: A.k.a]
#26421119 - 01/07/20 01:28 PM (4 years, 22 days ago) |
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Although I am just gearing up for my first grow, and have no working knowledge: I think we need to hear more about your grain prep. I'm thinking perhaps the moisture level wasn't where you need it one way or another, I'm thinking too wet.
At least that's more info we can get to draw conclusions.
-------------------- Dartho - Chaotic good 'Sith lord' elf from Elfwood, near Dreamland. Will force lightning tickle you until you pee your pants.
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Zachsonpub
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Quote:
Arkansashunter said: I am using a SAB and I sterilized the box after every few transfers just in case.
You'll read this time and time again, but you aren't trying to sanitize the box, you're just trying to keep the air still (Still Air box)... So don't wipe anything down between transfers unless you knock the box off your surface or something extreme. If that does happen, wipe shit down, let the air settle like you did at the start.
You've got it right on you agar plates it appears, so it may be the lids as mentioned before.
Quote:
One more question. Should I dump my whole agar plate into my jars? Should I remove the pin before dropping it in?
I dump it all, I don't care about pins. It should all be sterile, or close to it. I often have a plate per jar or 2 that I'll slice up, dump in the receiving grains and it's colonized in about a week.
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FreeCanadianHugs
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Registered: 08/07/19
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I think like the poster above me. SAB boxes are not sterile as much as weighted down the contaminates. Yes clean your SAB before use, but most importantly spray inside the SAB so the mist falls down and as it falls bonds with fine air particles and brings them to the floor of your SAB. This allows for a much cleaner working area at higher elevation in your SAB. When you bring things in and out of your SAB spray as you just created air currents that will disturb the contaminates and bring them up to a higher level in the SAB again.
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FreeCanadianHugs
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50th post
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SpunkyMonkey88
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Registered: 10/08/19
Posts: 1,331
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Re: 24/24 jars failed help a rookie? [Re: A.k.a]
#26421157 - 01/07/20 01:50 PM (4 years, 22 days ago) |
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Quote:
A.k.a said: Use the tyvek. When I was doing syringe to wbs jars I used tyvek with silicone on top then mp tape over it after injection just to be safe.
The only time a jar was a total loss I didn’t let the ship dry enough and when I took the jar out of the pc the silicon was stuck to the foil and pulled the tyvek off with it when I unwrapped it.
I just slapped micropore tape over the hole instead of putting tyvek back. It made it to the shake and then never recovered.
It still blows my mind how many oat jars go bacterial. Wbs forever for me.
What do you mean? You're saying there's a trend on here with oats and bacterial jars?
you got me here bro.
I really don't think the type of grain could ever dictate contamination rates.
If you PC your grains properly they should be 100% sterile.
Edited by SpunkyMonkey88 (01/07/20 01:50 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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No pats are horrible for bacteria, some grains contain more endospores than others
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Arkansashunter
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26421172 - 01/07/20 02:01 PM (4 years, 22 days ago) |
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I was feeling so dang confident after doing so well with the Agar I just couldnt figure out where I went wrong.
I'm very greatful for all of the response. I'll try to find something else to use as spawn my next visit to town.
I'm way out in the sticks as you could imagine oats were available.
Does anyone have any recommendations besides WBS. Nothing against WBS just incase i cant find a good source.
Thanks again everyone
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Wheat rye millet
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Zachsonpub
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26421190 - 01/07/20 02:08 PM (4 years, 22 days ago) |
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If you're in the sticks, chances are you have feeds stores around. I get millet at mine. So easy to work with. Oats easy af, too.
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HappyHigh
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Re: 24/24 jars failed help a rookie? [Re: Zachsonpub]
#26421250 - 01/07/20 02:39 PM (4 years, 22 days ago) |
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I would say you need to work on technique. I use no SAB just my kitchen, gloves, a spore print, and a butter knife. scrape off spores whisk in oats (24/hr after PC) use a lid with single layer 3m micrpore and even sometimes no lid ..tinfoil... no contams. i don't even use a breathing mask i do turn off all airflow, fans, a/c.
-------------------- Had I the heavens' embroidered cloths, Enwrought with golden and silver light, The blue and the dim and the dark cloths Of night and light and the half light, I would spread the cloths under your feet: But I, being poor, have only my dreams; I have spread my dreams under your feet; Tread softly because you tread on my dreams.
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SpunkyMonkey88
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Registered: 10/08/19
Posts: 1,331
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26421267 - 01/07/20 02:45 PM (4 years, 22 days ago) |
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Quote:
cronicr said: No pats are horrible for bacteria, some grains contain more endospores than others
Was this in response to my comment where i stated type of grain shouldn't play a role in contamination?
I get that one grain can have more (endo)spores then the next... but shouldn't a proper PC cycle kill them anyways?
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cronicr



Registered: 08/07/11
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Last seen: 2 years, 8 days
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In theory it should but often it doesn't
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Diego


Registered: 12/30/19
Posts: 346
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Quote:
SpunkyMonkey88 said:
Quote:
cronicr said: No pats are horrible for bacteria, some grains contain more endospores than others
Was this in response to my comment where i stated type of grain shouldn't play a role in contamination?
I get that one grain can have more (endo)spores then the next... but shouldn't a proper PC cycle kill them anyways?
Usually soaking them long enough will cause them to germinate so the pc will kill them
-------------------- Some do it for the income But we do it for the outcome Some of us are active While others just let their mouths run - Chali 2na & Jurassic 5
Edited by Diego (01/07/20 02:50 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: 24/24 jars failed help a rookie? [Re: Diego]
#26421286 - 01/07/20 02:56 PM (4 years, 22 days ago) |
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No hydrating the grain is all you need, longer soak times can result in more endospores than you started with. Once an endospores germinated it's no longer an endospores it's just bacteria, soaking softens everything making it easier to eradicate
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Diego


Registered: 12/30/19
Posts: 346
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26421300 - 01/07/20 03:00 PM (4 years, 22 days ago) |
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Quote:
cronicr said: No hydrating the grain is all you need, longer soak times can result in more endospores than you started with. Once an endospores germinated it's no longer an endospores it's just bacteria, soaking softens everything making it easier to eradicate
I soak 12 hrs then pc. Whats your method?
-------------------- Some do it for the income But we do it for the outcome Some of us are active While others just let their mouths run - Chali 2na & Jurassic 5
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: 24/24 jars failed help a rookie? [Re: Diego]
#26421303 - 01/07/20 03:02 PM (4 years, 22 days ago) |
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For oats? Hydrate and pc, they stopped carrying them here though so I'm using millet or wheat
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Diego


Registered: 12/30/19
Posts: 346
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26421313 - 01/07/20 03:06 PM (4 years, 22 days ago) |
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I buy wbs by the ton for my turkey so its what i use. Is it not the same method?
-------------------- Some do it for the income But we do it for the outcome Some of us are active While others just let their mouths run - Chali 2na & Jurassic 5
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Zachsonpub
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Re: 24/24 jars failed help a rookie? [Re: cronicr]
#26421314 - 01/07/20 03:06 PM (4 years, 22 days ago) |
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I was actually pissed with millet using a tek where you just add dry millet, water and a lil gypsum to jar then pc. My only prob was that I couldn't safely fit 10 in my presto as 3 would be on their side and would leak that water.
Recently was reading a book and they mentioned just putting a lid under the jar at the lid to prop them up during PC cycle. Something so obvious...
Anyway, sorry for the
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SpunkyMonkey88
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Registered: 10/08/19
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Re: 24/24 jars failed help a rookie? [Re: Zachsonpub]
#26421328 - 01/07/20 03:15 PM (4 years, 22 days ago) |
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Quote:
Zachsonpub said: I was actually pissed with millet using a tek where you just add dry millet, water and a lil gypsum to jar then pc. My only prob was that I couldn't safely fit 10 in my presto as 3 would be on their side and would leak that water.
Recently was reading a book and they mentioned just putting a lid under the jar at the lid to prop them up during PC cycle. Something so obvious...
Anyway, sorry for the 
Ha I dont let stuff like that bother me, I'm happy to fit 7 jars in my presto .. that's pretty dam good IMO...
Plus I dont like the idea of having jars on their sides... I'll just stick to the 7...
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Diego


Registered: 12/30/19
Posts: 346
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Quote:
SpunkyMonkey88 said:
Quote:
Zachsonpub said: I was actually pissed with millet using a tek where you just add dry millet, water and a lil gypsum to jar then pc. My only prob was that I couldn't safely fit 10 in my presto as 3 would be on their side and would leak that water.
Recently was reading a book and they mentioned just putting a lid under the jar at the lid to prop them up during PC cycle. Something so obvious...
Anyway, sorry for the 
Ha I dont let stuff like that bother me, I'm happy to fit 7 jars in my presto .. that's pretty dam good IMO...
Plus I dont like the idea of having jars on their sides... I'll just stick to the 7...
Cant you get a divider and put some pints up top? I have the same pc. Never done it but it looks like there's room
-------------------- Some do it for the income But we do it for the outcome Some of us are active While others just let their mouths run - Chali 2na & Jurassic 5
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