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OfflineArkansashunter
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Registered: 07/15/19
Posts: 56
Last seen: 3 years, 9 months
24/24 jars failed help a rookie?
    #26419717 - 01/06/20 05:32 PM (4 years, 23 days ago)

Hey guys,

Thanks in advance for any help.

I recently got into attempting cultivation and have done lots of research and felt like it was going well. Had good growth starting even though it was a little slow.

24 / 24 jars turned bad with what I believe (was told by community here) was Bacillus.
Awful smell in the jars smearing when shaking etc.

I have some agar plates that I did at the same time and have no noticable contamination.

I'm trying to figure out where I went wrong.
I did a whole lot of jars expecting to lose a few but not 100% failure.

I am using oats and pressure cooked them for 2 hours at 15psi.

I am using a SAB and I sterilized the box after every few transfers just in case.
I flamed sterilized between each transfer but that means going in and out of my box with my arms. Could this be my weak spot?

My jars were 3/4 full or maybe a little more. Am I failing due to poor GE? I have 2 holes in the lid with micropore tape over the holes. But I was wondering if my jars being too full limited the GE. Some jars were a little moist after PC could this be the culprit?

I probably have 6 agar plates with no obvious contaminants 3 of which are starting to pin in the container after 5 weeks.

Does anybody have any ideas where I'm dropping the ball before I waste these genetics?

One more question. Should I dump my whole agar plate into my jars? Should I remove the pin before dropping it in?

Sorry for the rambling mess. I'm just disheartened and looking for the holes in my game.

Thanks again


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InvisibleTormatoMFacebookDiscordReddit
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Re: 24/24 jars failed help a rookie? [Re: Arkansashunter]
    #26419746 - 01/06/20 05:53 PM (4 years, 23 days ago)

Using micropore tape as your GE filter is where you went wrong ....use polyfil or SFD in the future.

Dropping agar into jars I usually cut the agar into 6 wedges...1 wedge per jar...it's really up to you.

I remove the pins before dropping


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InvisibleDiego


Registered: 12/30/19
Posts: 346
Re: 24/24 jars failed help a rookie? [Re: Tormato]
    #26419754 - 01/06/20 05:58 PM (4 years, 23 days ago)

Ive never used oats but 2 hours seems like a long time


--------------------
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But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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Re: 24/24 jars failed help a rookie? [Re: Diego]
    #26419756 - 01/06/20 05:59 PM (4 years, 23 days ago)

Quote:

Diego said:
Ive never used oats but 2 hours seems like a long time



90-120 mins is the norm @ 15-18psi


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Helpful Threads

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Tormato's Q&A Thread Post Questions Here or PM me!

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Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


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InvisibleDiego


Registered: 12/30/19
Posts: 346
Re: 24/24 jars failed help a rookie? [Re: Tormato]
    #26419785 - 01/06/20 06:20 PM (4 years, 23 days ago)

Is this standard for all grains or just oats in particular?


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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Re: 24/24 jars failed help a rookie? [Re: Diego] * 1
    #26419792 - 01/06/20 06:26 PM (4 years, 23 days ago)

Quote:

Diego said:
Is this standard for all grains or just oats in particular?



All grains


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Helpful Threads

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Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


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InvisibleDiego


Registered: 12/30/19
Posts: 346
Re: 24/24 jars failed help a rookie? [Re: Tormato]
    #26419797 - 01/06/20 06:28 PM (4 years, 23 days ago)

Thanks dude


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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Re: 24/24 jars failed help a rookie? [Re: Diego]
    #26419805 - 01/06/20 06:37 PM (4 years, 23 days ago)

No prob!


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Helpful Threads

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Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


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OfflineArkansashunter
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Registered: 07/15/19
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Last seen: 3 years, 9 months
Re: 24/24 jars failed help a rookie? [Re: Tormato]
    #26420218 - 01/06/20 11:59 PM (4 years, 22 days ago)

Tormato
I hope I tagged you correctly thanks for the reply.

I have seen a Tek using the post office bags as a filter I would guess is technically a SFB. Is this sufficient or should I order something more official.

I'm not questioning your knowledge but I'm curious how confident you are that the poly tape was my issue? It would definitely explain the 100% failure. I just want to stop questioning myself. I dont want to lose the genetics.
Hope that was respectful enough. Thanks so much for your time Tormato.


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OfflineJohnRainy
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Registered: 07/09/19
Posts: 1,244
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Re: 24/24 jars failed help a rookie? [Re: Arkansashunter]
    #26420245 - 01/07/20 12:47 AM (4 years, 22 days ago)

Polyfill works like a hot damn.





Synthetic filter disks are very convenient and durable.


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OfflinecronicrFacebook
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Re: 24/24 jars failed help a rookie? [Re: JohnRainy]
    #26420247 - 01/07/20 12:52 AM (4 years, 22 days ago)

Micropore tape works for a lot of people but needs to be at least 2 layers.
So just to clarify you inoculated all jars  with wedges?


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: 24/24 jars failed help a rookie? [Re: cronicr]
    #26420308 - 01/07/20 02:49 AM (4 years, 22 days ago)

Quote:

Arkansashunter said:
Tormato
I hope I tagged you correctly thanks for the reply.

I have seen a Tek using the post office bags as a filter I would guess is technically a SFB. Is this sufficient or should I order something more official.

I'm not questioning your knowledge but I'm curious how confident you are that the poly tape was my issue? It would definitely explain the 100% failure. I just want to stop questioning myself. I dont want to lose the genetics.
Hope that was respectful enough. Thanks so much for your time Tormato.



If your drilling holes in the lids and just covering them with 1 layer of micropore tape that's going to be a failure point IMO. Even with 2 layers I still don't trust it like I do with polyfil or SFD....I'm old sorry :shrug:


Quote:

cronicr said:
So just to clarify you inoculated all jars  with wedges?




That was me :lol: Whole plate or I'll cut wedges...just depends what I'm working with


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Helpful Threads

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Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


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OfflinecronicrFacebook
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Re: 24/24 jars failed help a rookie? [Re: Tormato]
    #26420310 - 01/07/20 02:54 AM (4 years, 22 days ago)

He never actually said he used agar for the jars , just that he also had some going


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleYogiBear
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Registered: 08/24/19
Posts: 845
Re: 24/24 jars failed help a rookie? [Re: cronicr]
    #26420335 - 01/07/20 03:45 AM (4 years, 22 days ago)

My poly fill jars with a silicon injection port on oats and some results from oats in the background.

I had rye go bad like that. Smelled like sour mash.



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OfflineArkansashunter
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Registered: 07/15/19
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Re: 24/24 jars failed help a rookie? [Re: YogiBear] * 1
    #26420730 - 01/07/20 10:25 AM (4 years, 22 days ago)

Thanks for everyones time.

I did use agar in the jars. I cut them up with a flame sterilized scalpel and transferred a couple pieces in each jar.

The growth was going well in most jars but then turned for the worst.

I will pick up some poly fill tomorrow when I make it into the city.

I have been using micropore tape on the holes in my agar plates too. Should I bail on that as well?

Last menial question I have is tyvek an adequate material for SFD? Or should I wait for polyfill?

Thanks again Tormato and Cronicr


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OfflinecronicrFacebook
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Re: 24/24 jars failed help a rookie? [Re: Arkansashunter]
    #26420737 - 01/07/20 10:27 AM (4 years, 22 days ago)

No I use micropore on my agar jars it doesn't let contams through .
I would ditch the oats


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleDiego


Registered: 12/30/19
Posts: 346
Re: 24/24 jars failed help a rookie? [Re: cronicr]
    #26420847 - 01/07/20 11:32 AM (4 years, 22 days ago)

Quote:

Arkansashunter said:
Thanks for everyones time.

I did use agar in the jars. I cut them up with a flame sterilized scalpel and transferred a couple pieces in each jar.

The growth was going well in most jars but then turned for the worst.

I will pick up some poly fill tomorrow when I make it into the city.

I have been using micropore tape on the holes in my agar plates too. Should I bail on that as well?

Last menial question I have is tyvek an adequate material for SFD? Or should I wait for polyfill?

Thanks again Tormato and Cronicr



I
Quote:

cronicr said:
No I use micropore on my agar jars it doesn't let contams through .
I would ditch the oats



Does micropore tape work better than parafilm? It sure as hell is cheaper


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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OfflinecronicrFacebook
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Re: 24/24 jars failed help a rookie? [Re: Diego]
    #26420853 - 01/07/20 11:36 AM (4 years, 22 days ago)

No! It's a sticky mess on plates lol


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleDiego


Registered: 12/30/19
Posts: 346
Re: 24/24 jars failed help a rookie? [Re: cronicr]
    #26420886 - 01/07/20 11:50 AM (4 years, 22 days ago)

Quote:

cronicr said:
No! It's a sticky mess on plates lol



Ok. You said jar. My bad dude. Shit i thought I found a corner to cut! Haha


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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OfflineA.k.aM
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Re: 24/24 jars failed help a rookie? [Re: Diego]
    #26420897 - 01/07/20 11:56 AM (4 years, 22 days ago)

Use the tyvek. When I was doing syringe to wbs jars I used tyvek with silicone on top then mp tape over it after injection just to be safe.

The only time a jar was a total loss I didn’t let the ship dry enough and when I took the jar out of the pc the silicon was stuck to the foil and pulled the tyvek off with it when I unwrapped it.

I just slapped micropore tape over the hole instead of putting tyvek back. It made it to the shake and then never recovered.

It still blows my mind how many oat jars go bacterial.
Wbs forever for me.


--------------------
LAGM2020


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