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YogiBear



Registered: 08/24/19
Posts: 845
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YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙
#26418839 - 01/06/20 07:10 AM (4 years, 23 days ago) |
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YogiBear's No Pour Agar Tek ( this is a flow hood tek )
🍄🍄🍄🍄🍄🍄🍄🍄🍄

⚜️ THESE PP5 PETRIS ARE TOTALLY REUSABLE ⚜️ ⚜️ SIMILAR TO REGULAR PETRIS IN SHAPE AND FUNCTION ⚜️
👎 THEY DO FADE A LITTLE OVER TIME BUT NOT BAD 👎 👎 THE LIDS DO WARP A LITTLE 👎

⚜️ YOGIBEAR'S AGAR MIX ⚜️
Hanna Magnetic Stirrer (2in stir pill) 4 cup Pyrex Measuring Cup 250ml Cold Bottled Water Add 5g LME Stir 2-3 minutes Cloudy -> Microwave 2 mins on high Clear -> Stir in 5g Telephone Agar Add 3 Drops Food Color Stir well and Pour 12 plates (20cc each)

⚜️ WRAP 2 PLATES WITH ALUMINUM FOIL ⚜️

⚜️ LOAD IN THE PC ⚜️ ( 24 fit easily into a 16qt Presto )
⚜️ PC 30 MINUTES @ 17.6393645 PSI ⚜️

⚜️ LET IT DROP TO ZERO ⚜️
⚜️ TURN ON FLOW HOOD ⚜️
⚜️ COOL, WIPE AND BAG UP ⚜️ ( lay out to cool in front of flow hood ) ( quick wipe on lids for moisture w/paper towel ) ( a new paper towel is sterile, I use 1 per plate ) ( individually wrapped in sandwich bags ) ( OR use para-film or Saran Wrap instead if you'd prefer )

⚜️ CLEAN AND CLEAR ⚜️

⚜️ HOUSTON... WE ARE READY FOR LIFT OFF !!! ⚜️

🍄🍄🍄🍄🍄🍄🍄🍄🍄
AND REMEMBER TO RECYCLE AS MUCH AS YOU CAN ALL MY FOIL, PAPER TOWELS ARE USED ONCE IN THE LAB THEN THEY GO TO THE KITCHEN

Edited by YogiBear (02/06/20 10:48 AM)
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26419003 - 01/06/20 09:41 AM (4 years, 23 days ago) |
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Yo that's pretty cool and all but I'm all about pouring plates these days...
Sandwich bag thing seems sketchy tho, all that air in the bag is contaminated and those lids on those plates are loose AF...
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: SpunkyMonkey88]
#26419016 - 01/06/20 09:47 AM (4 years, 23 days ago) |
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Quote:
SpunkyMonkey88 said: Yo that's pretty cool and all but I'm all about pouring plates these days...
Sandwich bag thing seems sketchy tho, all that air in the bag is contaminated and those lids on those plates are loose AF...
I do everything in front of my flow hood so not too worried about that as I open the bag to let it flood with pure air. 👍
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26419037 - 01/06/20 10:06 AM (4 years, 23 days ago) |
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Quote:
YogiBear said:
Quote:
SpunkyMonkey88 said: Yo that's pretty cool and all but I'm all about pouring plates these days...
Sandwich bag thing seems sketchy tho, all that air in the bag is contaminated and those lids on those plates are loose AF...
I do everything in front of my flow hood so not too worried about that as I open the bag to let it flood with pure air. 👍
Oo ok ya then in that case no biggy as those baggies are manufactured with molten plastic and therefore be sterile on the inside...
So that's the only thing I could see being an issue..
Sweet idea tho... if i was doing no pours I'd much rather do these just for the sake of the clear lid.
So if you'd buying petris and all then why dont you just pour sterile plates? is it because you wanna be able to reuse them or something?
Side note: after realizing your a grandpa and now seeing how you are able to make your on GIFs and shit I'm seriously flabbergasted...I thought grandpa's and grandma's were supposed to have a hard Time even looking at a smart phone or computer haha... dude I dont even know how to do that shit!
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MrBovineJoni
Glip-Glop

Registered: 11/28/19
Posts: 264
Last seen: 13 days, 16 hours
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26419038 - 01/06/20 10:09 AM (4 years, 23 days ago) |
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Great write up - I always appreciate good quality pics; hope this helps someone out here. Don't take it the wrong way but the first thought that popped in my head when I saw this TEK - does he/she use it themselves even if they have flowhood available? That should make pouring agar with close to 100% sterile as possible
I have just recently started with pouring (not tried non-pours ever) because I was getting annoyed just by thinking about individually (or in packs of 2 or 4) packaging plates in sandwich bags. For bigger quantities or higher plate turnover rate this seems like a lot of busy work.
While I'm still far from perfect sterile technique - I prepare a large media bottle in advance and keep it in the fridge, then I microwave it and let it cool down for pouring - can get 20-30 plates done in a few minutes with a good success rate. Then I can store them later - then cool them down while still stacked in the SAB and put their 20 stack bag packet back on for storage (or use right away).
At the moment I'm at the "eager agar" mycology stage when I want to see and try lot's of stuff so I go through plates much faster than I would like to prep no-pours.
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: SpunkyMonkey88]
#26419523 - 01/06/20 03:28 PM (4 years, 23 days ago) |
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Quote:
SpunkyMonkey88 said:
Quote:
YogiBear said:
Quote:
SpunkyMonkey88 said: Yo that's pretty cool and all but I'm all about pouring plates these days...
Sandwich bag thing seems sketchy tho, all that air in the bag is contaminated and those lids on those plates are loose AF...
I do everything in front of my flow hood so not too worried about that as I open the bag to let it flood with pure air. 👍
Oo ok ya then in that case no biggy as those baggies are manufactured with molten plastic and therefore be sterile on the inside...
So that's the only thing I could see being an issue..
Sweet idea tho... if i was doing no pours I'd much rather do these just for the sake of the clear lid.
So if you'd buying petris and all then why dont you just pour sterile plates? is it because you wanna be able to reuse them or something?
Side note: after realizing your a grandpa and now seeing how you are able to make your on GIFs and shit I'm seriously flabbergasted...I thought grandpa's and grandma's were supposed to have a hard Time even looking at a smart phone or computer haha... dude I dont even know how to do that shit!
I have had jobs as a web designer, book publisher graphic artist and designed ads for one of those companies that sends coupon mailers to every house in America. That's where my eye for design and software comes from.
Also lots of network experience and network protection for old school internet service providers.
I only use iPhone for image work...
Try GIPHY CAM app for making gifs 😎👍
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: MrBovineJoni]
#26419543 - 01/06/20 03:42 PM (4 years, 23 days ago) |
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I do such small batches so I like the no pour tek the best
If I had a lab then yes, I'd do normal pours.
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Psicomb



Registered: 01/13/18
Posts: 4,635
Loc: the womb
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26419544 - 01/06/20 03:44 PM (4 years, 23 days ago) |
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What are you wiping your lids with? That seems to add an unnecessary risk
--------------------
When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something - nick sand
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: Psicomb]
#26419558 - 01/06/20 03:55 PM (4 years, 23 days ago) |
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Quote:
Psicomvb said: What are you wiping your lids with? That seems to add an unnecessary risk
I could just tilt and dump the moisture out onto a paper towel but I want to get as much out as possible so I just pop the lid up in the flow hood stream and use a fresh paper towel folded up to about 2in square and use the edge.
Definitely use a new paper towel for each petri 👍
Edited by YogiBear (01/07/20 06:19 AM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26419876 - 01/06/20 07:25 PM (4 years, 23 days ago) |
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So are these reusable? That’s a great price. Do you still have to wrap with parafilm or saran?
Also why 17.9 psi?
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: eatyualive]
#26419911 - 01/06/20 07:40 PM (4 years, 23 days ago) |
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: eatyualive] 1
#26420135 - 01/06/20 10:09 PM (4 years, 23 days ago) |
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Quote:
eatyualive said: So are these reusable? That’s a great price. Do you still have to wrap with parafilm or saran?
Also why 17.9 psi?
Yes these PP5 are reusable Eats and the 17.927456 is a complete playful joke my friend 😎. I guess I should edit the OP to reflect that.
I only use the sandwich bags because my hands are way to shaky to work with the para-film and wrap. I can just pop open a new bag in the flowhood stream, drop the petri in and done.
Edited by YogiBear (01/06/20 11:37 PM)
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Hobbyist
Citizen


Registered: 08/15/10
Posts: 805
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26420394 - 01/07/20 05:08 AM (4 years, 22 days ago) |
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Quote:
YogiBear said: I could just tilt and dump the moisture in some but I just pop the lid up in the flow hood stream and use a fresh paper towel folded up to about 2in square and use the edge. A new paper towel for each petri.
So you use a paper towel on the inside of your petri? Aren't the paper towels inherently unclean?
-------------------- Everything i say is completely hypothetical...
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: Hobbyist]
#26420443 - 01/07/20 06:24 AM (4 years, 22 days ago) |
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Quote:
Hobbyist said:
Quote:
YogiBear said: I could just tilt and dump the moisture in some but I just pop the lid up in the flow hood stream and use a fresh paper towel folded up to about 2in square and use the edge. A new paper towel for each petri.
So you use a paper towel on the inside of your petri? Aren't the paper towels inherently unclean?
Another job I had back in the day was as a body piercer and I was taught by the State health inspector that paper towels are sterile by nature of the manufacturing process as long as they are used once and tossed.
So I tear one off, fold it into a 2in square, wipe and done.
I will be making animated gifs in the next couple of days of this process. Just didn't have anyone to hold the camera for me when I did this write up.
Will bump the thread when the are added to the OP
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26420465 - 01/07/20 06:46 AM (4 years, 22 days ago) |
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Haha yeah I thought that was funny with the 17.9psi! :thimbup: good find.
Ok so you would still need parafilm or Saran Wrap for these if you were not using the plastic bag correct? Or do they kinda snap on?
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: eatyualive]
#26420475 - 01/07/20 06:52 AM (4 years, 22 days ago) |
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Quote:
eatyualive said: Haha yeah I thought that was funny with the 17.9psi! :thimbup: good find.
Ok so you would still need parafilm or Saran Wrap for these if you were not using the plastic bag correct? Or do they kinda snap on?
They are like regular petris and would need para-film, correct.
Updated the OP to reflect this.
Edited by YogiBear (01/07/20 07:05 AM)
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Hobbyist
Citizen


Registered: 08/15/10
Posts: 805
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26422358 - 01/08/20 04:35 AM (4 years, 21 days ago) |
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Quote:
YogiBear said:
Quote:
Hobbyist said:
Quote:
YogiBear said: I could just tilt and dump the moisture in some but I just pop the lid up in the flow hood stream and use a fresh paper towel folded up to about 2in square and use the edge. A new paper towel for each petri.
So you use a paper towel on the inside of your petri? Aren't the paper towels inherently unclean?
Another job I had back in the day was as a body piercer and I was taught by the State health inspector that paper towels are sterile by nature of the manufacturing process as long as they are used once and tossed.
So I tear one off, fold it into a 2in square, wipe and done.
I will be making animated gifs in the next couple of days of this process. Just didn't have anyone to hold the camera for me when I did this write up.
Will bump the thread when the are added to the OP
I gotcha. Well, do you keep your paper towels in a sterile environment or just sitting on the counter?
Wouldn't they pick up contaminations sitting in open air..?
-------------------- Everything i say is completely hypothetical...
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: Hobbyist]
#26422377 - 01/08/20 05:29 AM (4 years, 21 days ago) |
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Quote:
Hobbyist said:
Quote:
YogiBear said:
Quote:
Hobbyist said:
Quote:
YogiBear said: I could just tilt and dump the moisture in some but I just pop the lid up in the flow hood stream and use a fresh paper towel folded up to about 2in square and use the edge. A new paper towel for each petri.
So you use a paper towel on the inside of your petri? Aren't the paper towels inherently unclean?
Another job I had back in the day was as a body piercer and I was taught by the State health inspector that paper towels are sterile by nature of the manufacturing process as long as they are used once and tossed.
So I tear one off, fold it into a 2in square, wipe and done.
I will be making animated gifs in the next couple of days of this process. Just didn't have anyone to hold the camera for me when I did this write up.
Will bump the thread when the are added to the OP
I gotcha. Well, do you keep your paper towels in a sterile environment or just sitting on the counter?
Wouldn't they pick up contaminations sitting in open air..?
My body piercing room was tiny with no air flow when the door was shut so I would have to assume from that and the State Health Inspectors statement that's its both the room and paper towels being sterile at the manufacturing process. She just said "use fresh towels" for each procedure/person/client I had.
None of my customers ever got infections.
As the pictures above show after a 3 day waiting period... no contamination.
BTW I use proper Flow Hood technique and keep the lid I'm wiping closer to me when I wipe so there is no chance of paper towel "stuff" getting in the plate and always let the air flow blow on the inside of the lid for a second or two before putting the lid back on.
I will be making animated gifs for this procedure but I don't have a way rigged to hold my phone. Usually have one of my kids hold it for me and they are all still on vacation right now.
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Jive Ass Turkey
Stranger

Registered: 12/04/19
Posts: 36
Last seen: 2 years, 8 months
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26422840 - 01/08/20 11:48 AM (4 years, 21 days ago) |
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I've been using these for a while now. I bought the 75 and 100mm dishes. I just toss them in a big ziploc container with a vent (covered with tyvek and MP tape). I was positive they'd boil over but they all came out perfectly (of course not 100% if you count the condensation, lol). I've had some dishes sitting for over a month and saran wrapped with not signs of contam. I use about (I think) about 8-10ml agar mix in the 75 and 12.5ml in the 100mm.
I still have some prepped and laying around but switched to 4 and 8 oz mason jars because of how easy the agar just comes right off of them. Using SAGARI tek the agar sometimes sticks to plastics a little too much and makes it a pain.
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: Jive Ass Turkey]
#26423651 - 01/08/20 09:37 PM (4 years, 21 days ago) |
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Quote:
Jive Ass Turkey said: I've been using these for a while now. I bought the 75 and 100mm dishes. I just toss them in a big ziploc container with a vent (covered with tyvek and MP tape). I was positive they'd boil over but they all came out perfectly (of course not 100% if you count the condensation, lol). I've had some dishes sitting for over a month and saran wrapped with not signs of contam. I use about (I think) about 8-10ml agar mix in the 75 and 12.5ml in the 100mm.
I still have some prepped and laying around but switched to 4 and 8 oz mason jars because of how easy the agar just comes right off of them. Using SAGARI tek the agar sometimes sticks to plastics a little too much and makes it a pain.
Thanks Jive Ass Turkey. That's a creative solution indeed. So many ways to get things done.
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nevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 2 months, 6 days
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26424061 - 01/09/20 07:19 AM (4 years, 20 days ago) |
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So one could use these polypropylene petris for poured plates as well, and get around the wasted plastic issue, right?
-------------------- My LAGM 2020 Journal
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: nevereataamanita] 1
#26424155 - 01/09/20 08:42 AM (4 years, 20 days ago) |
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They are PP5 and totally reusable 😎👍
They are promoted as substitute for glass petris.
Edited by YogiBear (01/09/20 04:08 PM)
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TedsDead


Registered: 01/03/17
Posts: 4,998
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26424199 - 01/09/20 09:12 AM (4 years, 20 days ago) |
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thanks, yogi! might have to try these. I dont even wanna say how much i spent on plates last yr
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: TedsDead]
#26424293 - 01/09/20 10:04 AM (4 years, 20 days ago) |
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How’s the condensation with these things yogi?
-------------------- Tek's I use LAGM2020
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: staytrippy420]
#26424933 - 01/09/20 03:26 PM (4 years, 20 days ago) |
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Quote:
TedsDead said: thanks, yogi! might have to try these. I dont even wanna say how much i spent on plates last yr
I can see that for sure... this is my first big adventure past PastyPlates and wanted to get away from as many ongoing expenses on the front end of my setup. This does work good for me as I am small time but would hot pour plates if I had a tons to do.
Quote:
staytrippy420 said: How’s the condensation with these things yogi?
The condensation is about the same for almost any no pour so it's def humid on the lids (not horrible) and that's why I do the wipe with the paper towel after they cool. Out of my first 30 plates I had 2 that had a couple of drips of water on the agar.
TEK UPDATE
ANIMATED GIFS WERE ADDED FOR WIPING LIDS
STILL NEED A GIF FOR BAGGING THE PLATES... COMING SOON
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26425039 - 01/09/20 04:37 PM (4 years, 20 days ago) |
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Theses are great for pouring
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: cronicr]
#26425068 - 01/09/20 04:58 PM (4 years, 20 days ago) |
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So how do you go about reusing them? Sterilize? Run a pressure cooker cycle then repour?
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: eatyualive]
#26425228 - 01/09/20 06:38 PM (4 years, 20 days ago) |
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Quote:
eatyualive said: So how do you go about reusing them? Sterilize? Run a pressure cooker cycle then repour?
Since I do a "no pour" tek when I'm done with them I just do a quick soap wash, rinse, and dry, fill with fresh agar, PC, use, repeat.
I have never done a regular pour tek so I will let CRONICR answer for that procedure.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: eatyualive]
#26425336 - 01/09/20 07:52 PM (4 years, 20 days ago) |
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Quote:
eatyualive said: So how do you go about reusing them? Sterilize? Run a pressure cooker cycle then repour?
Filter patch bag pc them for later use
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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nevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 2 months, 6 days
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: cronicr]
#26441899 - 01/19/20 04:02 PM (4 years, 10 days ago) |
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I had an order of 24 of these PP5 petris arrive yesterday. I poured agar into 13 of them and transferred wedges to a couple of them. I had some observations that might be helpful to somebody else considering trying them out.
First of all, I was a bit surprised to see they arrive individually sealed in small plastic bags. That could be seen as a positive or a negative thing, I guess, but it's a bit more of a hassle than wiping down one long sleeve with iso, and making one scalpel slice to open them up.
Not a surprise, but they are significantly less clear than polystyrene petris. I also noticed they are slightly larger in diameter than the "100 mm" petris that I have been using.

This may have been mentioned earlier in the thread, but the sides of the lid comes down a lot farther over the bottom plate than on the petris I'm used to. The negative consequence of this is that I had a significantly harder time picking them up in one hand without lifting the lid inadvertently. This is because my fingers aren't making contact with the sides of the bottom plate--lifting just one dish works okay because I can squeeze the sides enough to pinch the bottom plate, but the taller the stack gets that harder it is to lift. Possibly could be solved by having a single lid on the bottom of a stack to just serve as a platform.

Another flaw is that the ring on top of the lid that facilitates stacking without tipping is lower profile than I'm used to. As a consequence, stacks are a lot more tenuous.
Finally, cling wrap doesn't stick to this plastic much at all, so you have to have your wrap pretty much all the away around before it starts to feel secure. A bit trickier to wrap but not impossible.
I'm going to keep trying to work with them because I like the idea of wasting less plastic (I do care about plastic waste, though less than my wife does, and anything I can do to avoid her getting annoyed with the hobby is probably worth the effort).
When it comes time to sterlize, I'm planning on doing so in a filter patch bag as cronicr recommends, and am hoping that just folding the bag and sealing with a binder clip will work?
Thanks for the heads up on these Yogi!
-------------------- My LAGM 2020 Journal
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: nevereataamanita]
#26442143 - 01/19/20 06:51 PM (4 years, 10 days ago) |
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I agree that they are not as clear as the normal petris for sure and I've been researching on different agar recipes to help with this very issues. I was using Stamets MEA mix and ditched it yesterday because it was too mucky. I don't wrap them so I didn't know about the sticking issue. Thanks for the heads up no that. I can add that to the tek on the next update.
The recipe that I'm going to use next is...
10g LME (light malt extract) 10g agar (telephone) 500ml of water
The picts I seen of these mixes are just about perfectly clear and should help get the best visibability from these plates.
https://www.shroomery.org/forums/showflat.php/Number/26440317#26440317
When I confirm this reciepe does indeed work I will update this tek to reflect the new mix 👍👍👍
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nevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 2 months, 6 days
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26442201 - 01/19/20 07:21 PM (4 years, 10 days ago) |
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That's pretty close to the recipe I use except less LME (8g). I don't think the clarity issue is all that bad, a lot better visibility than other no pour containers, right?
Edit: My biggest issue is picking them up in stacks inside a SAB... Just going to take some practice or some new tricks. I know you don't have to worry about that with your hood.
-------------------- My LAGM 2020 Journal
Edited by nevereataamanita (01/19/20 07:23 PM)
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: nevereataamanita]
#26442431 - 01/19/20 10:20 PM (4 years, 10 days ago) |
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Gotchya. You pour guys with SABs have my sympathy 💔🆘❗️
Yes. As much as I dig Pasty's plates as a first step as a beginner I'm more comfortable with a full on clear lid to see what's happening. Guys like Pasty can probably wave their hand over plates to see if they are contaminated 🤣 Sadly... I'm not there yet. 🥺
I will be getting media bottles before too long when I'm going full on but right now it's all experiments and don't need tons of plates.
It's funny how the whole mycology community can be affected by such a simple design difference as the height of the lid. I did wonder that myself when I got them but didn't blink since I'm using them like I am.
And why don't you have a FH young man? 🥰
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cronicr


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26442475 - 01/19/20 11:15 PM (4 years, 10 days ago) |
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Yes folding the bag with a binder clip is fine or zip ties ect
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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nevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 2 months, 6 days
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26442521 - 01/20/20 12:22 AM (4 years, 9 days ago) |
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Quote:
YogiBear said: And why don't you have a FH young man? 🥰
Man, it's a matter of space not cost! We have a very small house (which I actually really love) so my operation is confined to a shelf in a closet and I put the SAB on a kitchen counter when working. If I had a room (or walk-in closet) I could dedicate I would start building a hood in a second!
-------------------- My LAGM 2020 Journal
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: nevereataamanita]
#26442623 - 01/20/20 04:40 AM (4 years, 9 days ago) |
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I totally understand that. I divorced my wife a couple months ago and living with a friend in a spare bedroom so I get cramped.
I put my hood under a table and pull it out when needed.
It's my NO-SEE-UM FH 🤣
Edited by YogiBear (01/21/20 04:09 AM)
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nevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 2 months, 6 days
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26442992 - 01/20/20 11:09 AM (4 years, 9 days ago) |
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Oh man then I got no excuse!
-------------------- My LAGM 2020 Journal
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nevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 2 months, 6 days
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OK, so one more update. 100% of the first batch of PP5 petris went bacterial. Could be my shitty technique or maybe they didn't come sterile even though they were sealed. I'm going to PC them and try again.
-------------------- My LAGM 2020 Journal
Edited by nevereataamanita (01/28/20 08:57 AM)
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26471822 - 02/06/20 10:53 AM (3 years, 11 months ago) |
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UPDATED THE OP WITH MY NEW AGAR RECIPE AND PICTS
OP LINK HERE
Thanks for the help on getting clear plates everyone!!!
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#26471841 - 02/06/20 11:14 AM (3 years, 11 months ago) |
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I bought a bunch of these plates and eventually trashed them because they are too short at 11mm vs 15mm being standard height. This shortness makes them get more condensation on the lids when they cool and also the mycelium can end up growing right against the lid make it do weird things. You have to be more careful to pour just the right amount to keep the height down. Plus the visibility really is bad compared to a normal petri.
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YogiBear



Registered: 08/24/19
Posts: 845
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: sandman420]
#26471859 - 02/06/20 11:32 AM (3 years, 11 months ago) |
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Quote:
sandman420 said: I bought a bunch of these plates and eventually trashed them because they are too short at 11mm vs 15mm being standard height. This shortness makes them get more condensation on the lids when they cool and also the mycelium can end up growing right against the lid make it do weird things. You have to be more careful to pour just the right amount to keep the height down. Plus the visibility really is bad compared to a normal petri.
I use a 10cc syringe and put 20cc in each dish.
Using the LME/Telephone mix gives me clear plates compared to pre-made MEA mixes that I used in the past.
I do so few that I don't mind the clumsiness.
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greenladel

Registered: 05/27/20
Posts: 636
Last seen: 4 months, 13 days
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Re: YogiBear's No Pour Agar TEK (PP5 Petris) ❤️💛💚💙 [Re: YogiBear]
#27121536 - 01/02/21 05:20 AM (3 years, 26 days ago) |
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thank you for the tek 
i am curious how you get away with using 11mm high petris with a no-pour tek? i am currently doing the necessary learning before diving into agar and i have been seeing in general that people use deeper dishes to reduce splashing on the lid during the PC cycle. i would much rather use a shallow dish like petris with the convenience and safety of no-pour. what do you do different to them? or are they just being over-safe?
thank you
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