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Offline345536345
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Trying to get PE plates to pin
    #26401594 - 12/26/19 12:55 PM (4 years, 1 month ago)

I noticed a PE plate I recently made and kept around started to create what appear to be mycelial knots.  I'm trying to get it to fully pin - any tips?

This one plate has a bunch of bumps / knots while the others have little to none.  This plate also started to blue sooner than others although it has always looked clean.  I'm assuming the yellowed bumps there now are essentially aborts as they started as white knots that stopped growing.

I'm hoping to get it to pin.  I've misted it once a day (along with the other plates) to try and give them more favorable conditions.  It's purely a fun experiment so if it's all down the tubes it doesn't matter.  Any insight on how to proceed?  For reference, these fully colonized around 12/1.



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Offlineverum subsequentis
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Re: Trying to get PE plates to pin [Re: 345536345]
    #26401606 - 12/26/19 01:14 PM (4 years, 1 month ago)

Misting plates? Wow, i thought i'd seen it all. PE is a finicky variety and sometimes will only blob even given proper conditions. I'm not sure what to tell you about getting the plate to pin other that give it time.

So, are you aware that the plates are no longer sterile? That is, unless you took some pretty extreme steps to mist it with sterile water in a sterile environment.


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OfflineeLeSDenes
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Re: Trying to get PE plates to pin [Re: verum subsequentis]
    #26401607 - 12/26/19 01:16 PM (4 years, 1 month ago)

I have many PE plates from October and none of them are pinning yet, while the PE6 plates from November have all pinned. It just looks like it's taking longer and harder to get it to pin


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Offline345536345
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Re: Trying to get PE plates to pin [Re: verum subsequentis]
    #26401616 - 12/26/19 01:27 PM (4 years, 1 month ago)

Quote:

verum subsequentis said:
Misting plates? Wow, i thought i'd seen it all. PE is a finicky variety and sometimes will only blob even given proper conditions. I'm not sure what to tell you about getting the plate to pin other that give it time.

So, are you aware that the plates are no longer sterile? That is, unless you took some pretty extreme steps to mist it with sterile water in a sterile environment.




LOL!  Of course.  They're fully grown out and so I'd equate it to a colonized sub at this point.  I've already taken whatever wedges I needed long ago and this is just bonus round experimenting.  If it contams then whatever.

My prevailing thought was that the bluing was caused by lack of moisture and so I was trying to keep it going to the point of actual pins since it seemed to have "promise".    :grin:


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OnlineCamera93
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Re: Trying to get PE plates to pin [Re: 345536345]
    #26401802 - 12/26/19 03:53 PM (4 years, 1 month ago)



this took over a month to pin


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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Offline345536345
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Re: Trying to get PE plates to pin [Re: Camera93]
    #26401997 - 12/26/19 06:28 PM (4 years, 1 month ago)

Nice - did you do anything special or just let it sit?  I took off the parafilm surrounding the dish hoping some more FAE might help get it to pin but in the end it may have just accelerated the process to dry out the dish...


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OnlineCamera93
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Re: Trying to get PE plates to pin [Re: 345536345]
    #26402043 - 12/26/19 06:59 PM (4 years, 1 month ago)

just let it sit, those deli containers pry allow a ton of FAE


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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Offlinenewera
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Re: Trying to get PE plates to pin [Re: Camera93]
    #26402098 - 12/26/19 07:40 PM (4 years, 1 month ago)

Just leave It, my pe petris pin fine they just take awhile


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InvisibleCaps McGee
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Re: Trying to get PE plates to pin [Re: newera]
    #26402148 - 12/26/19 08:29 PM (4 years, 1 month ago)

Have you tried a proper casing?
:kittylaugh:

Quote:

newera said:
Just leave It, my pe petris pin fine they just take awhile



:whathesaid:

If its fully colonized, leave it unwrapped and popped open(no pours) itll pin eventually: patience is key in mush cult: PARTICULARLY with PE


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Offline99.99
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Re: Trying to get PE plates to pin [Re: Caps McGee]
    #26402197 - 12/26/19 09:31 PM (4 years, 1 month ago)

I couldn’t stop mine from pinning



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InvisibleCaps McGee
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Re: Trying to get PE plates to pin [Re: 99.99]
    #26402893 - 12/27/19 11:14 AM (4 years, 1 month ago)

It's not even fully colonized: likely stress fruiting Caused by contamination(bacteria of some sort imo)...

Edit: closer look, the center majority culture looks to be a mold of some sort: morphology is completely off for cubensis


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Offline99.99
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Re: Trying to get PE plates to pin [Re: Caps McGee]
    #26403230 - 12/27/19 04:34 PM (4 years, 1 month ago)

Fine ive got about 60 others that worked....ive moved on to better fungi APE are arngry little shits because  they’re all mutated and deformed



Golden teacher are much happier


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Offline345536345
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Re: Trying to get PE plates to pin [Re: 99.99]
    #26403558 - 12/27/19 07:46 PM (4 years, 1 month ago)

And as with most things in this hobby I guess I will just have to wait... 

:waitingpatiently:


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OfflineMH5109
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Re: Trying to get PE plates to pin [Re: 99.99]
    #26403560 - 12/27/19 07:48 PM (4 years, 1 month ago)

Just cloned 6 pe pins yesterday, the plates were inoculated 11/13 i believe, but only one group of plates pinned.


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Offline99.99
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Re: Trying to get PE plates to pin [Re: 345536345]
    #26403755 - 12/27/19 09:51 PM (4 years, 1 month ago)

Quote:

345536345 said:
And as with most things in this hobby I guess I will just have to wait... 

:waitingpatiently:




Very small agar plates would pin fast but that wont help you now 😪

For some reson everything i put on agar from fungi perecti pin


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Offline345536345
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Re: Trying to get PE plates to pin [Re: 99.99]
    #26416210 - 01/04/20 03:32 PM (4 years, 1 month ago)

Success!!! 



:idhitit:


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OfflineA.k.aM
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Re: Trying to get PE plates to pin [Re: 345536345]
    #26416344 - 01/04/20 05:04 PM (4 years, 1 month ago)

lol nice. Weird it pinned and bruised in the same spot.


99.99 are those cubes?? I assume so but that myc looks so thin.


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Offline99.99
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Re: Trying to get PE plates to pin [Re: A.k.a]
    #26416814 - 01/04/20 10:42 PM (4 years, 1 month ago)

Quote:

A.k.a said:
lol nice. Weird it pinned and bruised in the same spot.


99.99 are those cubes?? I assume so but that myc looks so thin.





Yes they are cubes... they are golden teacher the reason they look strange is fungi perfecti has change their agar Recipe
I didn’t like it at first but it’s actually easy to see contaminants instead of the mycelium being so dense you can’t see into it


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OfflineeLeSDenes
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Re: Trying to get PE plates to pin [Re: 99.99]
    #26416895 - 01/05/20 12:18 AM (4 years, 1 month ago)

got mine to pin a few days ago.


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