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OfflineCalicoCap
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Registered: 06/20/19
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Petri dish help *pics included* *updated 1/9
    #26414939 - 01/03/20 06:42 PM (4 years, 26 days ago)

Hey yall, i have a petri dish containing a PE6 transfer and it has quite a few yellow spots. I am wondering if its waste from the organism or mold.







Thank you thank you!


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Edited by CalicoCap (01/09/20 03:59 PM)


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OfflineKrip
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Re: Petri dish help *pics included* [Re: CalicoCap]
    #26415250 - 01/03/20 09:52 PM (4 years, 25 days ago)

They could be of mycelium metabolites or bacteria. Anyway, not normal, I would transfer from non yellow spot just to make sure, then look if more spots appear in the new transfer.

Keep us posted!


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OfflineMrBovineJoni
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Registered: 11/28/19
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Re: Petri dish help *pics included* [Re: Krip]
    #26415466 - 01/04/20 02:46 AM (4 years, 25 days ago)

Typically for dishes that you want to transfer you don't want the mycelium to cover more than 2/3 of the plate (ideally away from edges in the middle)so it does not touch edges (higher chance to pick up contamination). Transfer or preserve the plate by putting (extra wrap/bag can't hurt) it in a fridge for using later.

In the second pic (first pic is not very clear when zoomed in) I can clearly seen that the mycelium has reached the edge so it probably was good to transfer some time ago already but it looks rhyzomorphic and healthy apart from those suspect yellowish areas(could just be normal discolouration of agar. Could always just be metabolites but I have not seen many of them on healthy plates that are not very old in pictures here.

Kind of like in the thread here.

I add food colour to mine and after a few days some  areas that mycelium has colonised quite often lose colour and turn light brownish - as far as I have read this is normal (depends on the food colouring ingredients/type - some lose/change colour from mycelium).


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OfflineCalicoCap
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Re: Petri dish help *pics included* [Re: MrBovineJoni]
    #26417426 - 01/05/20 11:37 AM (4 years, 24 days ago)

Ill make some pastyplates today and isolate it. I let it grow out because of its spots, it developed one about 5 days in with a huge growth spurt. Ill try and isolate 3-4 pieces of it and will update in another post(maybe.)

Also the fact that it can eat the dye away is kind of wild.


Update! 1/9
Thanks for all your input yall and i think its only right that i post the results of the two transfers. Im a visual learner and always love to see a graphic when possible.

This is a transfer from a clean section of it. Thanks for the tips on how to do that.


This one came from one of the spots and as you can see its bad news bears. Its lost in the photo but its a dingy grey almost blue.



What i can take away from this is that i shouldnt give up on my agar work! I would have thrown it out a long time ago.


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Edited by CalicoCap (01/09/20 03:58 PM)


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