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InvisibleDeckardCain
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Registered: 09/22/12
Posts: 236
Cloning advice
    #26414756 - 01/03/20 05:19 PM (4 years, 26 days ago)

I’m looking to clone a fruit and put it to agar. I’ve tried 3 small small tissue peices but nothing is growing out, It’s been 4 days. I tried a bigger piece last night and it just looks like it’s going blue, and nothing growing out. All I want is fruiting myc to get off of multispore. Maybe the scalpel was too hot? I did cool it in agar. (Agar is 50/50 water and grain water) should I just take a pin and put it on a new plate even though there will be a lot of contamination on the pin? Or just try again ?


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OfflinecronicrFacebook
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Re: Cloning advice [Re: DeckardCain]
    #26414757 - 01/03/20 05:21 PM (4 years, 26 days ago)

Try a pin the growth is far more vigorous and will outrun contams


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InvisibleSir Pentinite
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Registered: 05/15/19
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Re: Cloning advice [Re: DeckardCain]
    #26414888 - 01/03/20 06:18 PM (4 years, 26 days ago)

Quote:

jerdope said:
(Agar is 50/50 water and grain water)




Is that a recipe that others use? It sounds pretty watered-down although I don't use grain soak water. I've had the scalpel hiss slightly when I cut a tissue sample, but it will still grow as long as there are some undamaged cells on the agar.


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- Terrence McKenna


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OfflineLevster
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Registered: 08/17/16
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Re: Cloning advice [Re: cronicr]
    #26415067 - 01/03/20 07:49 PM (4 years, 25 days ago)

Quote:

cronicr said:
Try a pin the growth is far more vigorous and will outrun contams




I was under the impression that we were to clone the healthiest mushroom (either stand alone or from a cluster) just before the veil breaks?


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OfflinecronicrFacebook
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Re: Cloning advice [Re: Levster]
    #26415090 - 01/03/20 08:08 PM (4 years, 25 days ago)

U certainly can...or u can clone pins


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It doesn't matter what i think of you...all that matters is clean spawn

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