|
c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
|
|
Quote:
Grimsweeper said: About 425-450ml per stack of 20 standard MEA. Yeah I don't know whats up I'm missing the plate pin party.
Are you doing the plate pin initiation dance?
If not that is probably the problem
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
Edited by c10h12n2o (08/05/20 01:51 AM)
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 13 minutes, 51 seconds
|
Re: Psilocybe natalensis [Re: c10h12n2o]
#26863968 - 08/05/20 08:09 AM (3 years, 5 months ago) |
|
|
This got me wondering so I went and dug up my plates. The tomentose plate is puffed up bad but I can see a pin pressed against the side, rhizo plate is blowing up I might have to take each of these to a plate.
--------------------
LAGM2020     
|
Nichrome
I'm a torso!



Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
Last seen: 1 hour, 42 minutes
|
Re: Psilocybe natalensis [Re: A.k.a]
#26864725 - 08/05/20 02:28 PM (3 years, 5 months ago) |
|
|
Shit, I've got LC that fruits lol.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
|
Grimsweeper
don't fear the sweeper


Registered: 01/29/18
Posts: 4,843
Loc: broom closet
Last seen: 6 days, 10 hours
|
Re: Psilocybe natalensis [Re: c10h12n2o]
#26864803 - 08/05/20 03:10 PM (3 years, 5 months ago) |
|
|
Quote:
c10h12n2o said:
Quote:
Grimsweeper said: About 425-450ml per stack of 20 standard MEA. Yeah I don't know whats up I'm missing the plate pin party.
Are you doing the plate pin initiation dance?
If not that is probably the problem
-------------------- When you clean a vacuum cleaner you are a vacuum cleaner Build yourself a Flow Hood in these 99 simple steps
 
|
One of Us
Stranger



Registered: 03/12/12
Posts: 1,140
Last seen: 6 days, 12 hours
|
|
I guess I never noticed the gill attachment until you guys pointed it out.

|
Grimsweeper
don't fear the sweeper


Registered: 01/29/18
Posts: 4,843
Loc: broom closet
Last seen: 6 days, 10 hours
|
Re: Psilocybe natalensis [Re: One of Us]
#26865619 - 08/05/20 10:55 PM (3 years, 5 months ago) |
|
|
What I've noticed about the veil is it breaks away from the cap then the stipe continues to grow far more than what you traditionally see in cubes or any species for that matter. So you'll have a mushroom 8 inches long with the veil remnants 4 inches down on the stipe.
Nothing picture worthy yet but the Melmac/Natal tub I crossed is moving pretty slow which leads me to believe they don't want to play nicely together. I have a melmac tub of the same ratio a few days behind it that's about to blow by.
-------------------- When you clean a vacuum cleaner you are a vacuum cleaner Build yourself a Flow Hood in these 99 simple steps
 
|
Solipsis
m̶a̶d̶ disappointed scientist


Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands
Last seen: 5 months, 18 days
|
|
Quote:
P. cubensis:

A=Basidiome B=Basidia C=Basidiospores D=Pleurocystidia E=Cheilocystidia
Quote:
Basidia 24-35 x 10-13 t~m , 4-spored, rarely 2- or 3-spored, hyaline, ventricose to subcylindric with a median constriction.
Pleurocystidia 18-30 x 6-13 t~m, scattered, thin walled, hyaline, ventricose, or subpyriform, or sometimes with acute apex, or submucronate with the apex obtuse to rounded.
Cheilocystidia 17-32 x 6- 10 t~m, abundant, forming a sterile band, hyaline, thin walled, fusoid-ventricose with obtuse to subcapitate apex, or mucronate, sornetimes sublageniform with long simple neck 4-18 t~m long, 3-5 t~m thick, but ocassionally irregularly branched.
https://www.mykoweb.com/systematics/literature/The%20Genus%20Psilocybe.pdf
Spores: https://upload.wikimedia.org/wikipedia/commons/thumb/d/dc/Psilocybe.cubensis.spores.jpg/1024px-Psilocybe.cubensis.spores.jpg
11.5–17 x 8–11 µm, subellipsoid - tho also seems if you look above, subhexagonal at the very least
Quote:
P. natalensis:
 
Gotta say i am not that experienced at this but don't the microscopic characteristics look quite similar (Despite some different words used for the morphology.)?
Can't really say what is too incongruent about the pleurocystidia and cheilocystidia.
Maybe just that the spores may have a different morphology. A little. Or not. Really need an expert or experienced mycologist to say if there is significant evidence here that these are not conspecific.
And/or just wait for the DNA analysis to potentially drive the point home? xD
Edited by Solipsis (08/06/20 03:09 AM)
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 13 minutes, 51 seconds
|
Re: Psilocybe natalensis [Re: Solipsis]
#26865967 - 08/06/20 08:07 AM (3 years, 5 months ago) |
|
|
Do you guys notice they get really soft and rubbery when they’re mature?
Totally forgot about this sweaterbox for the last week or two, didn’t miss much though.

Idk shit about microscopy stuff but on those charts aren’t the cube ones four pronged while natalensis looks like two and three?
--------------------
LAGM2020     
|
Solipsis
m̶a̶d̶ disappointed scientist


Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands
Last seen: 5 months, 18 days
|
Re: Psilocybe natalensis [Re: A.k.a]
#26866137 - 08/06/20 10:16 AM (3 years, 5 months ago) |
|
|
Nah both have 4-spored basidia but 2-spored and 3-spored are exceptions that occur with both. iirc
wow what are those crazies you are growing. I love crazies but i guess you didnt plan on dis.?
|
Celestialexplorer1



Registered: 03/25/20
Posts: 1,803
Loc: Floating in a tin can
Last seen: 2 months, 12 days
|
Re: Psilocybe natalensis [Re: Solipsis]
#26866291 - 08/06/20 11:25 AM (3 years, 5 months ago) |
|
|
Gotta be carful when pasturizing sand/coir and other casing in the same go because sand gets hotter quicker and if your monitoring the others and not that one your probably too hot.
--------------------
 To spend just one moment in eternity
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 13 minutes, 51 seconds
|
|
I just spawned the nastiest tub with the rest of my clone spawn.
I had like half a quart of coir left over and then while rounding up jars found an unopened three week old jar of over hydrated pasteurized manure compost that I made for pans.
I opened it and it looked clean and didn’t smell so I used it and once I got down into the middle of the jar I literally thought the dog shit under my chair for a minute.
So here we go clones!
--------------------
LAGM2020     
Edited by A.k.a (08/06/20 02:48 PM)
|
c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
|
Re: Psilocybe natalensis [Re: A.k.a]
#26867603 - 08/07/20 03:12 AM (3 years, 5 months ago) |
|
|
Bout to fruit a shoebox, is it a good idea to case these?
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
|
Grimsweeper
don't fear the sweeper


Registered: 01/29/18
Posts: 4,843
Loc: broom closet
Last seen: 6 days, 10 hours
|
Re: Psilocybe natalensis [Re: c10h12n2o]
#26867701 - 08/07/20 06:17 AM (3 years, 5 months ago) |
|
|
I've tried it every which way. IME casing and top layers blobbed worse but inconsistently. End results really seem to vary greatly on your culture.
-------------------- When you clean a vacuum cleaner you are a vacuum cleaner Build yourself a Flow Hood in these 99 simple steps
 
|
Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 3 days, 35 minutes
|
|
Yeah, I've had the same results with and without top layer. That's good and bad results from each. I finally got around to throwing this away. I thought it would never do anything. I guess I'll keep it around awhile longer. I've also got two with great surface conditions, no overlay, has knots and everything. Not doing a fuckin thing.
|
Grimsweeper
don't fear the sweeper


Registered: 01/29/18
Posts: 4,843
Loc: broom closet
Last seen: 6 days, 10 hours
|
Re: Psilocybe natalensis [Re: Crackatoa]
#26867855 - 08/07/20 08:22 AM (3 years, 5 months ago) |
|
|
Sucks to watch a tub reach maturity and still disappoint. My garden is loaded with old Subs that didn't do shit. Everything I have going now is from new MS so I have high hopes.
-------------------- When you clean a vacuum cleaner you are a vacuum cleaner Build yourself a Flow Hood in these 99 simple steps
 
|
Nichrome
I'm a torso!



Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
Last seen: 1 hour, 42 minutes
|
|
Is anybody trying outdoor beds?
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
|
One of Us
Stranger



Registered: 03/12/12
Posts: 1,140
Last seen: 6 days, 12 hours
|
Re: Psilocybe natalensis [Re: Nichrome]
#26868072 - 08/07/20 10:25 AM (3 years, 5 months ago) |
|
|
I don't think so, but a few of us have dumped contaminated subs outside and got fruits. They look much different.
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 13 minutes, 51 seconds
|
Re: Psilocybe natalensis [Re: One of Us]
#26868114 - 08/07/20 10:46 AM (3 years, 5 months ago) |
|
|
lol all my natal has been weird corkscrews. At least this guys not shredded.
I just checked the spawn date and blew my mind, 6-23. No wonder these are potent, talk about slow PE has nothing on these.

Idk if these things like restricted air or extreme humidity but on my other tubs pins only form on the bottom. I flip the sub then they mature and by then there’s new pins on the bottom again. The one sweater tub is about to be on its third flip.
--------------------
LAGM2020     
Edited by A.k.a (08/07/20 10:51 AM)
|
jbgtaa
extraterrestrial



Registered: 06/09/19
Posts: 1,785
Last seen: 3 years, 3 months
|
Re: Psilocybe natalensis [Re: A.k.a]
#26868293 - 08/07/20 12:43 PM (3 years, 5 months ago) |
|
|
my guys are really firm.
Also whats your box like? Just unmodded? I. Using those latching gasket boxes with the gasket taken out and unlatched, but lid on regular. I think they do like high moisture and CO2.
-------------------- If the thunder don't get ya, the lightning will. In another time's forgotten space, your eyes looked through your mother's face. Trade List Forever giving away prints. PM at anytime for a free print.
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 13 minutes, 51 seconds
|
Re: Psilocybe natalensis [Re: jbgtaa]
#26868776 - 08/07/20 05:10 PM (3 years, 5 months ago) |
|
|
Yeah just shoeboxes and the double size sweater boxes.
The sweater box is what’s really making me wonder cuz it’s flipped and there’s serious overlay which is now pressed into the bottom and sides with pins coming out of it. The myc overlay has got to be sealing off air.
I saved a spoonful of grain from my clone jar so I can hopefully make more spawn and try it with foil over the tub like a spawn run next.
Or maybe I’ll get lucky and get an actual pinset and just keep this culture going lol.
--------------------
LAGM2020     
|
|