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BlueAndOrange
Psychedelic success story…



Registered: 01/11/22
Posts: 1,076
Last seen: 2 hours, 13 minutes
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Quote:
BlueAndOrange said:
Quote:
BlueAndOrange said: Well, my charcoal agar plates have failed to inspire me, but they all do the same thing. I’m 100% sure it’s not contamination, but the wispiest mycelium ever. It’s almost impossible to photograph, but this is an inoculation plate with one drop from a MSS. In person it looks clean, just really thin weak mycelium.
I’m going to experiment with new agar recipes and restarting from spores, but this plate grew out clean looking. No obvious visible contaminants. So I sliced it up into quarters and dropped it into 4 quart jars of milo. Let’s see if this stuff can find some life in the grain.

I looked in on the grain jars this evening that I dropped this agar in. I can see what looks like mycelium growing from the agar into the grain in 2 of the 4 already. Maybe I’ll get nats out of these yet.
The pics are too crappy to see what’s happening. I’ll post some as soon as the mycelium grows out in the next couple days.
Still need to pour new plates to try again with fresh spores.
Okay, so tonight I’m seeing the mycelium growing into the grain. I think this experiment with the weak and wispy agar colonization might be working.
I might be moving towards nats!
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hummingbird

Registered: 06/29/14
Posts: 2,134
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Yeah, that's jumping off nicely!
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Rotnpins
🤮 Rotten-Pins 🍄



Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
Last seen: 1 year, 1 month
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Quote:
BlueAndOrange said:
Quote:
BlueAndOrange said:
Quote:
BlueAndOrange said: Well, my charcoal agar plates have failed to inspire me, but they all do the same thing. I’m 100% sure it’s not contamination, but the wispiest mycelium ever. It’s almost impossible to photograph, but this is an inoculation plate with one drop from a MSS. In person it looks clean, just really thin weak mycelium.
I’m going to experiment with new agar recipes and restarting from spores, but this plate grew out clean looking. No obvious visible contaminants. So I sliced it up into quarters and dropped it into 4 quart jars of milo. Let’s see if this stuff can find some life in the grain.

I looked in on the grain jars this evening that I dropped this agar in. I can see what looks like mycelium growing from the agar into the grain in 2 of the 4 already. Maybe I’ll get nats out of these yet.
The pics are too crappy to see what’s happening. I’ll post some as soon as the mycelium grows out in the next couple days.
Still need to pour new plates to try again with fresh spores.
Okay, so tonight I’m seeing the mycelium growing into the grain. I think this experiment with the weak and wispy agar colonization might be working.
I might be moving towards nats!

Very interesting.. I have a few similar plates.. I've been tempted to try the same thing to see what would happen. I'm glad you showed this. I may just have to give it a try.
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Encouraging. The reason I use grain broth for plates is to get the myc used to grain BEFORE it encounters it for the first time. The best results are coming from my current formula, which is 5g agar/5g LME/250ml fine strained grain broth. Probably the best recipe I've used for plates.
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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BlueAndOrange
Psychedelic success story…



Registered: 01/11/22
Posts: 1,076
Last seen: 2 hours, 13 minutes
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Quote:
PrimalSoup said: Encouraging. The reason I use grain broth for plates is to get the myc used to grain BEFORE it encounters it for the first time. The best results are coming from my current formula, which is 5g agar/5g LME/250ml fine strained grain broth. Probably the best recipe I've used for plates.
I will try this. I need to make up some agar and some grain jars.
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hummingbird

Registered: 06/29/14
Posts: 2,134
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Quote:
PrimalSoup said: Encouraging. The reason I use grain broth for plates is to get the myc used to grain BEFORE it encounters it for the first time. The best results are coming from my current formula, which is 5g agar/5g LME/250ml fine strained grain broth. Probably the best recipe I've used for plates.
Weird. I just tried some GWA plates, and it made everything I put to it look a lot like BlueAndOrange's charcoal plates. Really whispy and hard to see. Everything I transferred back onto my usual LME agar plates looks good. I used 10g agar to 500ml grain(oat) water.
Maybe if I added malt it would have worked better. I thought it might have been too high on nutrients and I should have halved it with regular water, but maybe it was too low on nutes.
My line of thinking was exactly what you said, plus I've seen/heard others speak highly of it.
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MysticMycologist
Dirt Sherpa



Registered: 10/14/21
Posts: 1,755
Loc: seeking samadhi
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Did you dilute the grain broth at all? If the agar is too high in nutrition, it can get very slow and wispy. That could definitely be the issue. GSA is my favorite, for the same reasons primalsoup mentions. I always dilute mine to around 50% strength though. I have even had good luck with as low as 20% grain broth. It is quite rich when full strength.
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Quote:
BlueAndOrange said:
Quote:
PrimalSoup said: Encouraging. The reason I use grain broth for plates is to get the myc used to grain BEFORE it encounters it for the first time. The best results are coming from my current formula, which is 5g agar/5g LME/250ml fine strained grain broth. Probably the best recipe I've used for plates.
I will try this. I need to make up some agar and some grain jars.
Here's the whole (current) description to use as a starting point:
Chop/grind 1/4 cup grain briefly (you want small chunks not flour), mix with 1 qt COLD water, put over med-high heat until reaches boil, immediately reduce to simmer (it WILL boil over rapidly), reduce to low-med heat and cook covered 15 minutes or so. Strain when done and refrigerate until use or freeze leftovers for later use. I dilute the result about 2:1 with tap water and strained it through the finest mesh filter I have (a tea strainer). This gets a pretty clear base for the agar.
GB/LME agar 5 g agar 250 ml strained grain broth agar (as above) 5 g LME
@Hummingbird, that's the result I had with grain water agar as well. Grain broth is a different beast as it gets into the good stuff inside the grains. Grain broth alone works pretty well, but grain broth + LME is the bomb.
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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hummingbird

Registered: 06/29/14
Posts: 2,134
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@MysticMycologist- Ok, I used the water from my grain soak...not diluted. It gets pretty dark, so I figured it was too high in nutrients...plus I know that higher nutrients in plates usually causes weaker looking mycelial growth.
@PrimalSoup- That's interesting. I've never heard of that route, but it sounds like it would work well. I sometimes add a pinch of grain/soy flour to my LME mix before cooking.
So, dilution with both grain water/broth. That's kind of what I was thinking. There seems to be conflicting info around on whether to dilute or not, but it makes sense to do so after seeing the results of not. Training mycelium to eat the grains you use before they hit the actual grains does seem like a good idea. I'll give it a shot again. Thanks for the info.
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CHUCK.HNTR
feral urbanite



Registered: 09/30/19
Posts: 2,255
Loc: SF, CA, USA
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Re: Psilocybe natalensis [Re: Forrester]
#27879951 - 07/27/22 11:08 PM (1 year, 5 months ago) |
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Quote:
nosf3r4tu said:
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CHUCK.HNTR said:
 Gonna start growing these soon, got a lot of thread history to catch up on!
Don't bother catching up. Just grow them like cubes and ask questions, if you have any.
Quote:
Forrester said: Lol, do take note that I said "shitty SAB", not "NO SAB". 
-------------------- "What is the practical application of a million universes?" -Alan Watts
   
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semilancreator
Stranger



Registered: 03/24/21
Posts: 54
Last seen: 3 days, 5 hours
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Re: Psilocybe natalensis [Re: CHUCK.HNTR] 6
#27880398 - 07/28/22 11:41 AM (1 year, 5 months ago) |
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Just wanted to drop these here. Natalensis fruiting outdoor in The Netherlands. Spent cake (clone culture on rye Spawn and coir) mixed with aged manure and a thin later of soil on top with some Ribwort Plantain as covercrop. Got these pics send To me while I am on vacation so cant Harvest Them atm but still I thought it was cool that a subtropical species fruits in the Netherlands on a rooftop!
Gotta fucking love the resilience of Natalensis
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Shewey
Crunchy


Registered: 04/15/22
Posts: 99
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Does anyone ever compensate jars. Im thinking of waiting a few days and taking a grain to agar then spawning it 1:1. Lighting sucks but im pretty sure its clean

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DERRAYLD
Constructus


Registered: 05/13/02
Posts: 9,284
Loc: South Africa
Last seen: 7 hours, 20 minutes
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Re: Psilocybe natalensis [Re: Shewey]
#27881358 - 07/29/22 06:04 AM (1 year, 5 months ago) |
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What do you mean by compensate?
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Guerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom
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Re: Psilocybe natalensis [Re: DERRAYLD]
#27881391 - 07/29/22 07:14 AM (1 year, 5 months ago) |
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Consolidate maybe?
-------------------- Being pissed on does not make you a real man.
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Shewey
Crunchy


Registered: 04/15/22
Posts: 99
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Re: Psilocybe natalensis [Re: Guerrilla]
#27881396 - 07/29/22 07:18 AM (1 year, 5 months ago) |
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Yea consolidate my bad
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Guerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom
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Re: Psilocybe natalensis [Re: Shewey]
#27881426 - 07/29/22 07:47 AM (1 year, 5 months ago) |
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Hell to the nah
-------------------- Being pissed on does not make you a real man.
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Shewey
Crunchy


Registered: 04/15/22
Posts: 99
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Re: Psilocybe natalensis [Re: Guerrilla]
#27881507 - 07/29/22 09:19 AM (1 year, 5 months ago) |
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Alrite spawning soon then
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smalltalk_canceled
Babnik



Registered: 07/13/20
Posts: 2,862
Last seen: 12 days, 7 hours
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Re: Psilocybe natalensis [Re: Shewey]
#27881777 - 07/29/22 01:56 PM (1 year, 5 months ago) |
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Natalensis "consolidates"itself lol
Even great looking grain colonization will usually have one hell of a aerial presence with NATS, at the top of the jar. It's majestic to open them sometimes.
Caveat is dryness, even NAT will leave a bone dry top without a shake
-------------------- Willpower is the one true virtue
  
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DoctorBenway
NatAlchemist


Registered: 06/08/22
Posts: 95
Last seen: 7 months, 28 days
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Quote:
smalltalk_canceled said: Natalensis "consolidates"itself lol
Even great looking grain colonization will usually have one hell of a aerial presence with NATS, at the top of the jar. It's majestic to open them sometimes.
Caveat is dryness, even NAT will leave a bone dry top without a shake
Ya lol i been running a bunch of brf pf tek cakes (waiting on this shoebox to do its thing) and the mycellia climbs right up thru the vermiculite filter xD legit had a set sitting 2 months cuz busy and i shit you not i literally had mycellia poking out of the nocc holes. I cant put cakes into 1/2 pint jars to dunk cuz that overlay just makes em into massive lump cakes lol, not that i think they need rehydration pretty sure that wild ass overlay creates a microclimate of its own... i birthed a set yesterday that wasnt fully colonized, had like a tiny spot yet at bottom of the jar after 8 days, but i was like fuck it... they gonna go faster if i put em out now hah
Edited by DoctorBenway (07/29/22 04:33 PM)
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smalltalk_canceled
Babnik



Registered: 07/13/20
Posts: 2,862
Last seen: 12 days, 7 hours
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If the bottom part is very bacterial, i just don't use this part. I take the good parts and leave the bacterial bottom, topping the bottom with coir. Have gotten some impressive fruiting from doing thus from very small amounts of grains.
Annoying to harvest, but economical
Since the bottom is struggling breaking them up is less meaningful.
It's no different than splitting a jar or a bag in two for making two subs
And it's common that bacterial jars are worst at the bottom
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (07/29/22 05:23 PM)
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