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OfflineFridgedoor
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Re: Psilocybe natalensis [Re: Fridgedoor] * 4
    #27856980 - 07/10/22 06:43 AM (1 year, 6 months ago)

On another note, I just went through some of my pics from previous grows and found one which shows some of my personal record Nat fruits. Unfortunately I can't remember if I ever weighed them...



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OfflineBlueAndOrange
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Re: Psilocybe natalensis [Re: YoshiTrainer] * 1
    #27857074 - 07/10/22 09:22 AM (1 year, 6 months ago)

Quote:

YoshiTrainer said:
What are you using for agar?




10g agar, 10g LME, hint of charcoal, 500ml water.


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Invisiblebridge2far


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Re: Psilocybe natalensis [Re: BlueAndOrange] * 1
    #27857079 - 07/10/22 09:30 AM (1 year, 6 months ago)

Maybe reduce the LME a bit. Just compared your recipe with D3’s. Maybe try…
10g agar
8g LME
500 ml water

If you’re using tap water, maybe try distilled water.


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OfflinePrimalSoup
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Re: Psilocybe natalensis [Re: BlueAndOrange] * 2
    #27857456 - 07/10/22 02:51 PM (1 year, 6 months ago)

Quote:

BlueAndOrange said:
Quote:

YoshiTrainer said:
What are you using for agar?




10g agar, 10g LME, hint of charcoal, 500ml water.




There's something they don't like given how they're growing and I'd blame the charcoal.  Try adding some grain water, or grain broth water (the latter made from boiling ground grain not whole) or some nutritional yeast. 

I'm now using 9g agar, 10g LME, and 500ml broth water.  They grow OK without the LME but aren't nearly as happy about it.  But before that it was 9g agar, 10g LME, 1g yeast and 1g rye flour + 500ml tap water and that always worked quite well, but I'm still working on the ideal formulation...

Edit oh shit - made the wrong mistake.  They don't like NOT having LME but will grow without it, so I'm putting it back in along with the broth water, if that helps.  But LME + yeast + rye flour worked fine.  Sorry for the spaced confusion there.



Edited by PrimalSoup (07/10/22 10:29 PM)


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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: PrimalSoup] * 2
    #27857534 - 07/10/22 03:39 PM (1 year, 6 months ago)

Yeah I thought it was mold at first, but all those plates look like normal germination with extremely weak growth.


I only ever use agar and lme but I have had some similar issues when I cook the agar up and then don’t use it. A couple times it seems like it must have separated out or something and some plates had tons of lme, others none, and also the stiffness was all over plate to plate.



Derrayled I can’t imagine 8g. If I was going to go that high it would be natal though, I have very similar experiences to what you described. Even the tendency to dwell on gnarly stuff from the past on cubensis. Natalensis was just pure joy and gratefulness with surprising coherency even during the peak.


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Invisiblehummingbird

Registered: 06/29/14
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Re: Psilocybe natalensis [Re: A.k.a]
    #27857719 - 07/10/22 05:53 PM (1 year, 6 months ago)

I was thinking maybe the charcoal too. I've never used charcoal in plates, but I've seen people do fine with it. Anyone in here using it in their mix for nats?

I just recently made my first GWA plates, and my germination(cubes) plates look really weak and whispy with those halos around the more visible myc too. I figured it had too much nutrition and I should have cut it with regular water before adding the agar...all the transfers I made back onto MEA look normal.

I always switch it up a little, but it's usually something like-
10-11g agar
7-8g malt
500ml tap water

Sometimes I'll add a pinch of soybean or grain flour, yeast, or this beer yeast nutrient mix I have, but not always.


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Invisiblesandman420
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Re: Psilocybe natalensis [Re: DERRAYLD] * 1
    #27857732 - 07/10/22 05:59 PM (1 year, 6 months ago)

Quote:

DERRAYLD said:
I took 4g Nats last night and then a further 4g a few hours later




fucking legend


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InvisibleBigwormS
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Re: Psilocybe natalensis [Re: DERRAYLD]
    #27857770 - 07/10/22 06:10 PM (1 year, 6 months ago)

Quote:

DERRAYLD said:
I took 4g Nats last night and then a further 4g a few hours later, sat in total darkness with ear buds in listening to carbon based lifeforms.

At this point I feel like Natalensis are a gift, I'm feeling a bit dissociated this morning because it really was one of the most amazing experiences I've had.
All oev in the dark, I traveled so far, saw so much but saw so little.

Nats are sneaky shape shifters as well, oev in the dark transitions into extreme cross over between waking dreams and then I realse it's outsmarted me and my eyes are closed again.
Once I got past the initial urge to sleep and pushed through the drowsiness everything changed and I became one with the universal ooze that exists all around us, the unconscious universal flow that we're all a part of.

Some strange realizations about my voyage and the loneliness of being but the amazing beauty and happiness being within the transcendental state.

It was and is the most relaxing,  bright, glowing,  positive mushroom I've taken for a long time.
The thought loops are non existent, I love mushrooms but situations in my life sometimes make me focus on the past and I get stuck in negativity, Nats just never do this, I never get stuck in a negative or concerning loop, it's just bliss and amazing visuals.

14 hrs later and I wouldn't mind doing it all again.




Damn, I hear 1g dry is good, 4g sounds like you were searching for God and beyond. This sounds like an amazing shroom! I never hear any negative about the experience. I can't wait till I get to try these out. I'm waiting on my spores to wake up on agar now.


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OfflineDoctorBenway
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Re: Psilocybe natalensis [Re: Hillbillyjohn] * 1
    #27857966 - 07/10/22 09:17 PM (1 year, 6 months ago)

I noccd a set 6*21 and birthed last wednesday they hadnt grown the tissue layer yet like the others i had started 5*19 (had to wait to birth due to vacation) when i put them out there was a few dense patches starting which is how the tissue layer began on the others and resembles how sclerotia looks to me invitro... anyways this is what those brf cakes are doing that didnt form the tissue layer invitro
L
The liquid isnt metabolites, water splashed on it when getting it out for pic xP also dark spot on the tissue is from bumping the glass as well and itll prolly bruise a bluish green by tomorrow... i have some lumps on another cake that are doing that xP weird stuff


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OfflineBlueAndOrange
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Re: Psilocybe natalensis [Re: hummingbird] * 2
    #27857991 - 07/10/22 09:33 PM (1 year, 6 months ago)

Quote:

hummingbird said:
I was thinking maybe the charcoal too. I've never used charcoal in plates, but I've seen people do fine with it. Anyone in here using it in their mix for nats?

I just recently made my first GWA plates, and my germination(cubes) plates look really weak and whispy with those halos around the more visible myc too. I figured it had too much nutrition and I should have cut it with regular water before adding the agar...all the transfers I made back onto MEA look normal.

I always switch it up a little, but it's usually something like-
10-11g agar
7-8g malt
500ml tap water

Sometimes I'll add a pinch of soybean or grain flour, yeast, or this beer yeast nutrient mix I have, but not always.




Alright. I’m pouring new plates this week. Using the agar and malt.

I’ll make some potato flake agar too while I’m at it. I have 4 media bottles. No charcoal, no food dye. Maybe things will get growing.

Had a 4g GT trip today that came with the message: you don’t need higher doses, you need better mushrooms.

I’m trying to get there.


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Offlinesan pedro guy
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Re: Psilocybe natalensis [Re: BlueAndOrange]
    #27857997 - 07/10/22 09:37 PM (1 year, 6 months ago)

Have you ever tried brf pucks?? Or making brf cakes with your syringe then you could make your own spore prints and have more control on how clean your spores are…or at least get a few grams you could give them a whirl while you zero in on the agar issue :shrug: just a thought….they are well worth the wait tho!


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OfflineBlueAndOrange
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Re: Psilocybe natalensis [Re: san pedro guy]
    #27858013 - 07/10/22 10:01 PM (1 year, 6 months ago)

Quote:

san pedro guy said:
Have you ever tried brf pucks?? Or making brf cakes with your syringe then you could make your own spore prints and have more control on how clean your spores are…or at least get a few grams you could give them a whirl while you zero in on the agar issue :shrug: just a thought….they are well worth the wait tho!




I’m determined to get these growing on agar and going to grain jars. Maybe I should learn to do a brf puck while I’m doing it.

I don’t think my spores are particularly dirty, I think my agar recipe is wrong for these. Lots to do this week I guess.


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Offlinesan pedro guy
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Re: Psilocybe natalensis [Re: BlueAndOrange]
    #27858037 - 07/10/22 10:35 PM (1 year, 6 months ago)

I might be mistaken but I thought the pucks grew similar to agar and could be donors back onto agar…


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Invisiblehummingbird

Registered: 06/29/14
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Re: Psilocybe natalensis [Re: san pedro guy]
    #27858044 - 07/10/22 10:41 PM (1 year, 6 months ago)

You could totally do that, or even inoculate grains with it too if you wanted...


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OfflinePrimalSoup
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Re: Psilocybe natalensis [Re: hummingbird] * 1
    #27858067 - 07/10/22 11:03 PM (1 year, 6 months ago)

Quote:

hummingbird said:
I was thinking maybe the charcoal too. I've never used charcoal in plates, but I've seen people do fine with it. Anyone in here using it in their mix for nats?

I just recently made my first GWA plates, and my germination(cubes) plates look really weak and whispy with those halos around the more visible myc too. I figured it had too much nutrition and I should have cut it with regular water before adding the agar...all the transfers I made back onto MEA look normal.

I always switch it up a little, but it's usually something like-
10-11g agar
7-8g malt
500ml tap water

Sometimes I'll add a pinch of soybean or grain flour, yeast, or this beer yeast nutrient mix I have, but not always.





Charcoal has to be the right kind, I've used it in the distant past because it was supposed to encourage germination but it never seemed to make much difference.

OTOH have a look at this plate poured last week (the last one it only got a partial fill) that I exposed to the kitchen air for a few seconds as a test of growth potential.  It's the new formula 9g agar 10g LME and 500ml grain broth.  Looks like a winner - don't see the lipstick bacteria colonies very often that's for sure!



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Invisiblehummingbird

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Re: Psilocybe natalensis [Re: PrimalSoup]
    #27858094 - 07/10/22 11:49 PM (1 year, 6 months ago)

Some nice diversity there!


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OfflinePrimalSoup
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Re: Psilocybe natalensis [Re: hummingbird]
    #27858108 - 07/11/22 12:05 AM (1 year, 6 months ago)

It kind of floored me, since I had opened it up for like ten seconds altogether.  Caught a bit of penicillin around the edge (glass petri) before I wrapped it up to keep all that, uhm, goodness inside the dish where it belongs. :laugh2:


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if you stand too close to the machine it'll start to eat you
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OfflineDERRAYLD
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Re: Psilocybe natalensis [Re: PrimalSoup]
    #27858110 - 07/11/22 12:06 AM (1 year, 6 months ago)

Nice color on the lipstick mold.


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OfflineBlueAndOrange
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Re: Psilocybe natalensis [Re: PrimalSoup] * 1
    #27858122 - 07/11/22 12:18 AM (1 year, 6 months ago)

I have charcoal agar plates with FP+ growing nice and strong. Seems to be working there.

Either way, straight agar and LME as well as potato flake agar are both things I can make easily with ingredients on hand, so I’ll try those this week.


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InvisibleMysticMycologist
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Re: Psilocybe natalensis [Re: BlueAndOrange] * 4
    #27858466 - 07/11/22 10:32 AM (1 year, 6 months ago)

5th flush on the outdoor natalensis. They come up almost after every rain. They are not pretty, but they make us melt.


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