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OfflineConsistentPublic
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Registered: 06/15/22
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Last seen: 1 year, 6 months
Re: Psilocybe natalensis [Re: karri0n]
    #27820947 - 06/15/22 03:39 PM (1 year, 7 months ago)

Thanks for responding.

While I cannot completely exclude the latent spores theory I find it highly unlikely as just a day or two prior to putting the spores in question to agar I did a deep clean of my lab space including running an air purifier for several days, sanitizing my SAB with alcohol, a day in the sun, and again with a 10% bleach solution. Used fresh sterile swabs only opened in the SAB, and did solipsis’ trick of immobilizing contams with soapy water inside the SAB. The lab space had been cleaned in this manner at least three times since I had tampanensis spores out. I also inoculated two other plates from the same print at the same time that grew out with normal nat fruits. The jar producing these stones was actually spawned before the other two, so it wasn’t a long waiting period like tamps.

When I found them I did a bit of research on it as I thought it was novel. I found an old post on mycotopia I believe of someone else with nats producing stones, and I believe there was another post on Reddit and even one on here from a few years ago of someone else with nat stones iirc so it’s not completely unheard of. I can try to find links later. I’ll post updates as I have them.


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OfflineConsistentPublic
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Re: Psilocybe natalensis [Re: A.k.a]
    #27820960 - 06/15/22 03:51 PM (1 year, 7 months ago)

I plan on both cloning and trying to fruit directly from stones in new sub after a drying out period. I’ve tried to wait these out in this tub but it’s going on 1.5-2 months in sub, and the stones haven’t grown in a week or two.


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OfflineConsistentPublic
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Re: Psilocybe natalensis [Re: V.L]
    #27820963 - 06/15/22 03:57 PM (1 year, 7 months ago)

Thanks for the reply

This tub already smells like nats and cubes. But I’ll double check the sclerotia when I harvest.


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OfflineConsistentPublic
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Re: Psilocybe natalensis [Re: Adas] * 1
    #27820967 - 06/15/22 03:59 PM (1 year, 7 months ago)

I’ve squeezed the ones protruding from the sub, and they are firm, just like other stones.


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Offlinesmalltalk_canceled
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Re: Psilocybe natalensis [Re: ConsistentPublic]
    #27820968 - 06/15/22 04:01 PM (1 year, 7 months ago)

Nice development anyhow.

Nats blowing up the home grower community in so many ways


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Edited by smalltalk_canceled (06/15/22 04:50 PM)


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InvisibleWall.E
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Registered: 06/05/20
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Re: Psilocybe natalensis [Re: smalltalk_canceled] * 1
    #27821001 - 06/15/22 04:36 PM (1 year, 7 months ago)

I’m not saying you’re wrong and Alan is available on this site, just send him a pm. I’m sure he’d love to sequence a nat stone.

On a similar note I thought I had an albino or leucistic tamp when it turned out to be a natalensis contamination





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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: Wall.E] * 1
    #27821016 - 06/15/22 04:47 PM (1 year, 7 months ago)

Lmao do you know how that happened??


There’s an old thread on here somewhere about cube and oyster “contamination” in grows.


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OfflineSirPsycho
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Re: Psilocybe natalensis [Re: A.k.a] * 1
    #27821169 - 06/15/22 06:24 PM (1 year, 7 months ago)

Well, this has taken a turn and then another turn.

That shit is hilarious wall.e


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InvisibleWall.E
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Re: Psilocybe natalensis [Re: SirPsycho] * 1
    #27821200 - 06/15/22 06:54 PM (1 year, 7 months ago)

No idea but it’s stature and cap had me believing I was onto something new and exciting, but nope, just the best contam someone could ask for.

No idea where I fucked up


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Life’s shit, but I’m loving it



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OfflineConsistentPublic
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Re: Psilocybe natalensis [Re: Wall.E] * 3
    #27821407 - 06/15/22 08:46 PM (1 year, 7 months ago)

https://

Harvested the Psilocybe natalensis sclerotia. Will be cloning the largest two sclerotia which were 19.7 and 18.6 grams respectively. The total weight of stones from one quart of spawn is 190.37 grams. Will take some others and allow them to dry for a week or two in the lab, then rehydrate and put to substrate to try for fruits.  There are at least a couple dozen small sclerotia that would make good samples for others to try growing them from.


Edited by ConsistentPublic (06/15/22 08:48 PM)


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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: ConsistentPublic] * 2
    #27821584 - 06/15/22 10:51 PM (1 year, 7 months ago)

Idk man that looks exactly like tamp stones.


I’m definitely curious but just can’t believe it without seeing fruits.


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InvisibleMycelium Juice
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Re: Psilocybe natalensis [Re: A.k.a] * 2
    #27821671 - 06/15/22 11:42 PM (1 year, 7 months ago)

Quote:

A.k.a said:
Actually just cloning to a plate should answer the question, tamp and natal have vastly different mycelium.




I agree with A.k.a :smoker:

An agar plate could have this answered pretty fast


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OfflineConsistentPublic
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Re: Psilocybe natalensis [Re: Mycelium Juice]
    #27821948 - 06/16/22 05:44 AM (1 year, 7 months ago)

https://https://mycotopia.net/topic/110649-psilocybe-natalensis-communal-grow/page-3

Here’s a mycotopia thread from last year where someone (newmoon) found natalensis sclerotia on brf cakes. It’s not completely novel, I just got lucky as hell to find them.


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InvisibleGuerrilla
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Re: Psilocybe natalensis [Re: ConsistentPublic]
    #27821971 - 06/16/22 06:07 AM (1 year, 7 months ago)

:waitingpatiently:


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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: Guerrilla] * 3
    #27822022 - 06/16/22 06:49 AM (1 year, 7 months ago)

I still think what was in the jars in that thread was just the big blobby myc that grows being compressed against the jar.

There was still no pictures of harvested stones anywhere, just jars with the stone looking growth on the glass, and they were no where near tamp sized hunks.



That said I wouldn’t be totally surprised if there was some subspecies that had developed sclerotia. I’ve definitely had overlay blobs that were halfway between mycelium and mushroom flesh.


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OfflineForresterM
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Re: Psilocybe natalensis [Re: A.k.a] * 1
    #27822078 - 06/16/22 07:19 AM (1 year, 7 months ago)

:callingbullshit:

Kidding... But I'm on the side saying that's gotta be a culture mixup or errant spore.


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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineConsistentPublic
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Re: Psilocybe natalensis [Re: A.k.a]
    #27822087 - 06/16/22 07:25 AM (1 year, 7 months ago)

Well I’ll keep posting updates as I have them. Cloning today and setting some aside to dry out for another attempt at fruits from the sclerotia.


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OfflineConsistentPublic
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Re: Psilocybe natalensis [Re: Forrester]
    #27822114 - 06/16/22 07:48 AM (1 year, 7 months ago)

Culture mixup is impossible. I had harvested my tamp sclerotia before I put these to agar. They did not cross paths at all. Errant spore is very unlikely as well due to multiple deep cleanings of the lab between the time I put tamp spores to agar and these nat spores. 4 months and at least three deep cleans in between them. I also use Solipsis’ technique of using soapy water to immobilize contams inside the SAB rather than ineffectively trying to kill them with iso. I started three other nat plates at the same time, two from the same print and one from swab labeled as natal super strength which turned out to be 100% natalensis. The myc looked identical on all plates from the same print. Had no clue it was gonna produce sclerotia until I went to spawn to bulk and thought I had bacterial contam till I started finding small stones. They were started on agar April 7, moved to grain on April 18, and spawned to bulk May 10.


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InvisibleWall.E
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Re: Psilocybe natalensis [Re: ConsistentPublic] * 4
    #27822157 - 06/16/22 08:32 AM (1 year, 7 months ago)

Quote:

ConsistentPublic said:
Culture mixup is impossible.




Look at this guy, the first human to never make a mistake


Edited by Wall.E (06/16/22 08:34 AM)


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OfflineConsistentPublic
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Re: Psilocybe natalensis [Re: Wall.E] * 2
    #27822166 - 06/16/22 08:46 AM (1 year, 7 months ago)

I’m not saying I’m infallible, but it is physically Impossible to mix up cultures when you only have the one going at a time. Im capable of reading quite well and could clearly read the print. Just because it hasn’t happened for yall (sclerotia from nats) doesn’t mean it can’t happen. Keep Occam’s razor in mind with everything, the simplest solution with the least moving parts is most often the correct one. I know what I inoculated with, it had been proven before this run. The tamps and nats were months apart temporally and latent spores from a tamp print that was used once months prior surviving multiple deep cleans of the lab is even more incredible than nats producing stones. I have more evidence to prove this as legit then y’all have trying to poke holes. The myc on agar looked identical to other nat plates going at the same time that produced fruits. The substrate and stones smell like nats/cubes not mexicana or tamps. I’m taking the steps to verify they are indeed natalensis sclerotia. And again what benefit would there be for me to lie about any of this? I also willing to get samples to anyone else who wants to work towards verifying it.

So maybe stop being naysayers and maybe try helping to verify this radical development that can vastly change the understanding of Psilocybe natalensis as a species. I thought this place was supposed to be the forefront of mycological experimentation.


Edited by ConsistentPublic (06/16/22 08:51 AM)


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