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chris77
Archaic Revivalist



Registered: 01/17/22
Posts: 1,868
Last seen: 6 hours, 18 seconds
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Quote:
Mr.Giggles said:
Quote:
smalltalk_canceled said: If a prostitute ever had a Shoebox
edit: vegan sex worker
Will the vegan prostitutes still eat your meat?
at least they might chew it?
-------------------- the observer is the observed j. krishnamurti
Edited by chris77 (06/02/22 12:44 AM)
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 24 minutes
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Quote:
bridge2far said:
Quote:
RiseOMorpheus said: Bridge2far,for whatever reason I didn’t think to ask what your surface conditions look like? When I ran the nats in a pasty ezdial, it kept beads on top of the overlay. It was kinda weird looking to me but the culture liked it! Guess it’s no different than wanting beads on your sub, because essentiallly the overlay has become the surface of the sub.
My conditions have been the same as what you describe. Even at max fae, it maintained moisture beads on the overlay. Any cube would have thrived in those conditions. Wish I knew what variable to fuck with to piss these nats off just enough to initiate pinning.
Overlay Consumption Report:
Ended up with 24 dry grams of overlay. Pulverized a bit and consumed 0.3g (my usual microdose weight). 15 minutes later I felt the first hints of lightness of head & body. Aaaaaaaaaaaaand that’s about all. The dose did boost my mood & motivation, which is what I hope to get from every microdose. But it was a very mild experience. Positive but mild.
Time for me to streak more plates and try again.
Was it comparable to your microdoses of fruit?
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LAGM2020     
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bridge2far


Registered: 10/09/21
Posts: 382
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Re: Psilocybe natalensis [Re: A.k.a] 1
#27802504 - 06/02/22 10:05 AM (1 year, 7 months ago) |
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Quote:
A.k.a said: Was it comparable to your microdoses of fruit?
This is my initial attempt to grow nats. So, I don’t yet have any experience microdosing nat fruit.
Compared to my usual microdose experience, the nat overlay was remarkably weaker.
Typical microdose for me is 0.3-0.4g b+. I’ve come to expect excessive yawning and a profound light feeling in my head & body for about an hour. During that hour I want to tune out the world, close my eyes, and breathe deeply. I’ll feel mentally sharp & internally motivated to power through my never-ending to-do list for the rest of the day and on through the afterglow day.
The nat overlay did provide mental sharpness & internal motivation all day yesterday but no afterglow feeling today. The overlay felt remarkably less potent & less profound than my usual experience. But, I have 24g of this overlay now. I’ll throttle up the dose until it equals my usual experience. I’m excited to get a better feeling of how the effects of nats differ from cubes.
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Rayfellow
Stranger



Registered: 10/30/21
Posts: 84
Last seen: 1 year, 27 days
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very curious if different species and even strains have a notable difference in effect when microdoseing. IV kinda been close minded and never believed anyone was able to actually tell a different in effect when mycro doseing. I have become much more open to the idea I had some Ovid's and the trip n afterglow we're incredible n I credibly different for me compared to any cubensis I ever had. this is a comparison with macro doses but opened my eyes to how wonderfully mushrooms are and how much more depth there is to them then ever thought there could be. I totally beilve Mico doseing different species are intirerly possibility for different effects between em and how they interact n effect us mentally n phisicaly. it's kinda been a profound lesson and draws me I to find out more and more and hear outhers experience s n thoughts and recommendations of spesies with vary ing effects.
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san pedro guy
Captain



Registered: 10/22/17
Posts: 1,806
Last seen: 1 year, 3 months
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Re: Psilocybe natalensis [Re: Rayfellow] 1
#27803047 - 06/02/22 05:36 PM (1 year, 7 months ago) |
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Second flush 2 days after 1st

Also, is this normal in nat world?
-------------------- Noob Grow Along 2022
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Ankhseramun
Wanderer



Registered: 10/11/21
Posts: 589
Loc: Cantina, Mos Eisley, Tatooine
Last seen: 1 year, 7 months
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I had one jar out of 10 do this to me and it was clearly bacterial. I top fruited it not to screw up all the others.
Edited by Ankhseramun (06/02/22 05:39 PM)
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san pedro guy
Captain



Registered: 10/22/17
Posts: 1,806
Last seen: 1 year, 3 months
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Gotcha…i usually don’t mix jars anyway…prob do a 1:1 ratio…i haven’t had many issues with bacterial spawn and from some of the early grows on this thread it looks as tho nats don’t give af about nothin!
-------------------- Noob Grow Along 2022
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 24 days, 19 hours
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Quote:
san pedro guy said: Gotcha…i usually don’t mix jars anyway…prob do a 1:1 ratio…i haven’t had many issues with bacterial spawn and from some of the early grows on this thread it looks as tho nats don’t give af about nothin!
They don't. Most of my nat grain jars have been bacterial cause my SAB sucks, they always work fine.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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san pedro guy
Captain



Registered: 10/22/17
Posts: 1,806
Last seen: 1 year, 3 months
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Re: Psilocybe natalensis [Re: Forrester] 1
#27804494 - 06/03/22 05:09 PM (1 year, 7 months ago) |
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coo! thanks for the reassurance, i’m finally going to test these out tomorrow morning, let y’all know how it goes…
-------------------- Noob Grow Along 2022
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,901
Loc: Rent free in your head
Last seen: 36 minutes, 27 seconds
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Alright, I'm gonna join in on he fun.

Here's some nat on millet. Wasn't even fully colonized yesterday and it's already all "let me out". This is day 7 from A2G, shaken at inoculation and 3 days in.
Gonna use this to inoculate 4 all in one bottles based of Muda's Bottle TEK coir recipe
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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MysticMycologist
Dirt Sherpa



Registered: 10/14/21
Posts: 1,755
Loc: seeking samadhi
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Re: Psilocybe natalensis [Re: SirPsycho] 1
#27804564 - 06/03/22 05:49 PM (1 year, 7 months ago) |
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I love how fast natalensis colonizes. It outran my fastest cube(Cambodian Gold clone) on grain and colonizing coir. Fruiting can be a little slow though. I bet it will be awesome in a bottle tek.
-------------------- Two eyes to look, One eye to see. Prying open my third eye 
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PBJ710
Strangler


Registered: 07/05/19
Posts: 2,623
Last seen: 18 days, 13 hours
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Re: Psilocybe natalensis [Re: SirPsycho] 3
#27804593 - 06/03/22 06:06 PM (1 year, 7 months ago) |
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Fingers crossed for something special from this plate that came from a clone of green worms.

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BlueAndOrange
Psychedelic success story…



Registered: 01/11/22
Posts: 1,076
Last seen: 4 hours, 58 seconds
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Re: Psilocybe natalensis [Re: PBJ710]
#27804774 - 06/03/22 07:58 PM (1 year, 7 months ago) |
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Seeing germination of what I hope is mycelium and not mold on my first nat plates. So excited to join the nat club!
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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So was natalensis ever sequenced or did the couple scientist just assume it's a new species?
-------------------- OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum... There are known knowns, there are known unknowns, there are also unknown unknowns. With great privilege comes great responsibility.
  Quantom Qups PROOF AND Soft Drops Turn your Swab to a Syringe and Syringe to Multiple Syringes! No Pours (QuantomStyal)Magic Fruit Leather DMT for IandI
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Guerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom
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Re: Psilocybe natalensis [Re: QM33]
#27805270 - 06/04/22 05:53 AM (1 year, 7 months ago) |
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Quote:
QM33 said: So was natalensis ever sequenced or did the couple scientist just assume it's a new species?
It was confirmed as new, but close to Cubensis.
https://www.shroomery.org/forums/showflat.php/Number/27501471#27501471
-------------------- Being pissed on does not make you a real man.
Edited by Guerrilla (06/04/22 05:54 AM)
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Quote:
Alan Rockefeller said: Psilocybe natalensis ITS sequence is now in Genbank:
https://www.ncbi.nlm.nih.gov/nuccore/OK491080
Okay found this, which seems to be the most up to date genetic info on this mushrooms.
What's the difference between this and cubensis?
Alan?
And I understood it was close. But what differences were recognized during sequencing I think would be really valuable information, and one of our moderators/members should have that info I believe?
Also besides the original documentation by the couple researchers, and Alan's tests, which don't seem to be revealed, there is nothing?
** Lol for a while I was getting nats and tamps confused, like their latins names.
I'm sure you've all seen the natal X cube project that's going around on Facebook. Looks promising. But it wouldn't surprise me to find out that nats are cubes. And it would make total since that the first "interspecific" hybrid we see in the psychadelic realm would be one that's easier to achieve by more closely related specimens.
** Wait......
https://www.shroomery.org/forums/showflat.php/Number/27501474
I'm going to compare these to the other trees I've seen..
Edited by QM33 (06/04/22 05:59 AM)
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Psilocybe natalensis [Re: QM33]
#27805279 - 06/04/22 06:06 AM (1 year, 7 months ago) |
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Nats have different active compounds. They’re genetically similar to cubes, but different. I know this might shock you QM, but mushrooms can look the same but be different species.
People have been crossing species of fungi for awhile.
Facebook is a nice place, why don’t you go live there instead?
-------------------- Life’s shit, but I’m loving it
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: Psilocybe natalensis [Re: QM33]
#27805290 - 06/04/22 06:16 AM (1 year, 7 months ago) |
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Quote:
Alan Rockefeller said: Someone sent me a Psilocybe natalensis ITS sequence a few months back, so I had a pretty good idea what P. natalensis was - but it wasn't my sequence so I didn't want to share it. This week I made a Psilocybe natalensis sequence in my lab, which matches the other P. natalensis sequence I have. I've also uploaded it to Genbank, the first sequence of this species there.
The strain is Natal Super Strength.
Photos of the collection: https://mushroomobserver.org/472561
Here is a tree I made which includes P. natalensis and related species, with P. allenii as an outgroup.

The Psilocybe cf. cubensis sequence is interesting - it's quite close to cubensis, and has some noise, but I don't think it's just a bad read of cubensis because it has some of the same nucleotide substitutions that Psilocybe ovoideocystidiata has. Gaston Guzman collected it in Nepal in October of 2001. Without studying the collection (it's in XAL) I can't tell if it's a new species or if it's something which hasn't been sequenced yet like Psilocybe pseudoaztecorum.
Other things you can see in this tree are that Penis Envy has the same ITS sequence as regular cubensis, Psilocybe ovoideocystidiata from the east coast is the same as it is on the west coast and Psilocybe thaiaerugineomaculans is the same as Psilocybe thaiduplicatocystidiata.
In case you'd like to experiment with making phylogenetic trees or analyze the natalensis sequence yourself, here's the FASTA file: http://images.mushroomobserver.org/natalensis.fas
I would. I can't seem to download it tho, is the link still good? Ide like to understand the key genetic differences between these two mushrooms.
Waleee
"" Nats have different active compounds. They’re genetically similar to cubes, but different. I know this might shock you QM, but mushrooms can look the same but be different species.
People have been crossing species of fungi for awhile.
Facebook is a nice place, why don’t you go live there instead?
What different compounds? Link? And nats actually don't necessarily look the same to me, similar sure, but not the same. You know organisms can actually look different and be same species?
Ya people having been doing alot for awhile. And theirs a big difference between mushrooms, fungi and interspecific hybrids.
Because I live here, and it's full of idiots like you, and sadly you retards have been leaking in here faster than we can get you out.. what a world.
Ban 
I could find several links documenting misclassification of fungi.
Edited by QM33 (06/04/22 06:17 AM)
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 12 days, 9 hours
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Re: Psilocybe natalensis [Re: QM33]
#27805292 - 06/04/22 06:22 AM (1 year, 7 months ago) |
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Many of your questions are answered in this thread, read back
-------------------- Willpower is the one true virtue
  
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Psilocybe natalensis [Re: QM33] 1
#27805293 - 06/04/22 06:23 AM (1 year, 7 months ago) |
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Which classification did Alan get wrong? I don’t care about Guzman or Stamets getting a mushroom wrong, they did that shit all the time. As far as I know Alan is pretty much the gold standard for sequencing and classification.
He’s also a mod here, does that make him more believable in that smooth brain of yours?
-------------------- Life’s shit, but I’m loving it
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