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OfflineHindsight
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Re: Psilocybe natalensis [Re: Southerner] * 4
    #27611598 - 01/08/22 11:49 AM (2 years, 20 days ago)

It looks like bruising to me but it's tough to tell for sure. Could be the way your tub is setup, the bottom left corner is getting too much dry air from the heater.

Here's another one of those crazy plates that are tomentose as the base layer, and then random rhizo overlays. I put this one to grain today:


Two of my shoeboxes have fully colonized:




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Offlinemistermushly

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Re: Psilocybe natalensis [Re: Hindsight]
    #27612127 - 01/08/22 08:17 PM (2 years, 19 days ago)

Giving P.Natalensis a go - so far without luck ...

I have no problem getting great results with cubes and I treated the Nat the same, as far as I understand that's the proper way -

multi-spore syringe to petri to LC to oats then colonized -
added to Coir + Verm in a dubtub at an average of 75f

It's now about a month in of being in fruiting conditions and I'm going to retire them -

Everything smelled fine but took on a kind of orange hue, with some weird greenish bruising and stalled like this at week 2.   





Could this be contamination or poor growing conditions or preparation?
I'm thinking I may try to obtain a new starter print or MS... these seem fun and don't quite feel like giving up on them yet.


Edited by mistermushly (01/08/22 08:23 PM)


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OnlineA.k.aM
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Re: Psilocybe natalensis [Re: mistermushly]
    #27612165 - 01/08/22 08:56 PM (2 years, 19 days ago)

That’s odd looking.


I think it’s probably dirty, but possibly some other species judging by the color and the weird growth on the sides.


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OfflineHindsight
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Re: Psilocybe natalensis [Re: A.k.a] * 1
    #27612186 - 01/08/22 09:12 PM (2 years, 19 days ago)

Yeah that looks really dirty to me - note all the uncolonized areas of coir visible through the sides of the tub. Your syringe produced good looking growth on agar? Any reason you went to LC from there instead of just knocking up your grain jars with agar wedges? Saves time and reduces vectors for contamination. You sure about your coir hydration? Didn’t go too wet? How did your jars look? Any pics of them?


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Offlinemistermushly

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Re: Psilocybe natalensis [Re: A.k.a]
    #27612270 - 01/08/22 10:11 PM (2 years, 19 days ago)

Quote:

A.k.a said:
That’s odd looking.


I think it’s probably dirty, but possibly some other species judging by the color and the weird growth on the sides.





Thanks AKA and Hindsight -

My typical flow is to create an LC right away from agar which seems to make duplicating a successful grow easier,
though this next round, I'll go with a wedge as you (A.K.A.) suggested.

The mycelium was kind of cotton-y and not too rhizomorphic, but I didn't think it was necessarily unhealthy. I didn't take a pic of the colonized jar, but it looked typically white - similar to cube.

The LC turned out kind of cloudy - but not sure if that's a definitive sign of contams - a lot of the cube LC's crank out healthy flushes that look similar...



Thanks for the advice! - I'll keep you posted on the next round. hoping to meet these guys in person!


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OfflineStrech
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Re: Psilocybe natalensis [Re: mistermushly] * 2
    #27612316 - 01/08/22 11:12 PM (2 years, 19 days ago)

I'll join in on the fun.



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OfflineMelgo
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Re: Psilocybe natalensis [Re: Strech] * 3
    #27612422 - 01/09/22 04:26 AM (2 years, 19 days ago)

My first ever Natalensis shoebox:



I've just put the shoebox into fruiting conditions, 10 days after spawning.
1 jar of spawn for 3 jars of substrate (CVG); and 1,5 jars of "casing" (CVG).

Fruiting temperatures are 18°C during the day and 20°C at night (winter here).
Let's see what's going to happen in the next few days - I'm very excited about this!
:awedance:

This is the mycelium it came from:


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OnlineA.k.aM
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Re: Psilocybe natalensis [Re: Melgo]
    #27612613 - 01/09/22 08:48 AM (2 years, 19 days ago)

Looks like you got the good type of overlay.


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OfflineForresterM
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Re: Psilocybe natalensis [Re: A.k.a]
    #27612723 - 01/09/22 09:57 AM (2 years, 19 days ago)

What's the bad type look like?


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OfflineMelgo
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Re: Psilocybe natalensis [Re: A.k.a]
    #27612734 - 01/09/22 10:08 AM (2 years, 19 days ago)

Quote:

A.k.a said:
Looks like you got the good type of overlay.




Cool!

It's thanks to you, actually :hatsoff:
Pages back in this thread you advised to select the most tomentose mycelium possible, and I did so.

I had some agar rounds where the mycelium got 1 to 2 centimeters thick, I didn't put any of these to grain.

I have one more jar where the mycelium was wispy on agar (but not as fast a colonizer as the one above). I'll spawn it soon and see what happens.


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InvisibleSoutherner
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Re: Psilocybe natalensis [Re: Melgo]
    #27613134 - 01/09/22 03:36 PM (2 years, 19 days ago)

Thanks Hindsight. It’s starting to form knots so I’m going to let it rock unless I see it getting crazy.

Does anyone have a print from some Nats that consistently overlay they’d be willing to swap? I have yet to have any overlay from these and interested in trying it. My original genetics are from crack.


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InvisibleMycoWeek
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Re: Psilocybe natalensis [Re: MycoWeek] * 5
    #27613374 - 01/09/22 07:25 PM (2 years, 18 days ago)

Here are progress of my grow:

Crazy mycelium, I got holes too close to surface, many area were dead and dried out, so I decided to take a fork and scrape off those dense mycelium.


Recovered nicely after scraping


Pinning, and covered with mycelium again.


About time to harvest, these mofos looks like Enoki.


1.5 inches of fluffy mycelium that feels like cotton candy.  pretty easy to harvest, where not too much substrate are attached to mushrooms. Simply pull and snip with a scissor.


Edited by MycoWeek (01/11/22 01:19 PM)


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Invisiblemilkboy
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Re: Psilocybe natalensis [Re: MycoWeek]
    #27613459 - 01/09/22 08:45 PM (2 years, 18 days ago)

Looks like your tub got snowed on :lol:



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OnlineA.k.aM
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Re: Psilocybe natalensis [Re: milkboy]
    #27613955 - 01/10/22 10:36 AM (2 years, 18 days ago)

I just caught a snippet of the nat discussion on discord the other day.


There does seem to be two main lines, one from grim that has a lot more instances of crazy overlay, and the other that psilopsychin and crack had that put up the crazy canopies of skinnier fruits.


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InvisibleTheDoobskerS
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Re: Psilocybe natalensis [Re: A.k.a]
    #27614175 - 01/10/22 02:10 PM (2 years, 18 days ago)

Nice that this is now confirmed. A lot of us assumed there were 2 lineages.

Then mine and any spores I've given out will be from that Crack lineage.

Does anybody know the origin? Did both lineages come from the same wild specimen or have there been multiple Natalensis collected from the wild?

I think it has been going around as a cube variety for years, right? Until recently when it was sequenced


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InvisibleMelliferous
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Re: Psilocybe natalensis [Re: TheDoobsker]
    #27614188 - 01/10/22 02:22 PM (2 years, 18 days ago)

Quote:

TheDoobsker said:
Nice that this is now confirmed. A lot of us assumed there were 2 lineages.

Then mine and any spores I've given out will be from that Crack lineage.

Does anybody know the origin? Did both lineages come from the same wild specimen or have there been multiple Natalensis collected from the wild?

I think it has been going around as a cube variety for years, right? Until recently when it was sequenced



Zif would know about that -- I think that's where these all came from but I could be wrong. Haven't followed it too closely


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InvisibleMycoWeek
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Re: Psilocybe natalensis [Re: A.k.a]
    #27614219 - 01/10/22 03:00 PM (2 years, 18 days ago)

Quote:

A.k.a said:
I just caught a snippet of the nat discussion on discord the other day.


There does seem to be two main lines, one from grim that has a lot more instances of crazy overlay, and the other that psilopsychin and crack had that put up the crazy canopies of skinnier fruits.




Lol. I guess mine exhibits both traits?😂


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OfflineFailboat
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Re: Psilocybe natalensis [Re: MycoWeek]
    #27614234 - 01/10/22 03:14 PM (2 years, 18 days ago)

Mine are still just fruiting in petris but I've seen long skinny fruits and wonderful twisty nat fruits. I think I need to take transfers from all my plate fruit clone plates and get some super clean growth before moving on. Running through some back log still. Too much old mediocre work slowing down newer better spawn, but if it don't mold it's worth while.

I need more shelves and a whole lot of cleaning everything.:lol:


Edited by Failboat (01/10/22 03:15 PM)


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OfflineArtemisia_Ale
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Re: Psilocybe natalensis [Re: Failboat]
    #27614275 - 01/10/22 04:17 PM (2 years, 18 days ago)

About to start that Rosecomb culture onto some grain and see if it carried over. The growth looks incredibly uniform, and not as fluffy... should be less overlay (I've heard someone here say clone-cultures don't seem to get it so bad anyway). Starting that penistyle one as well!


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InvisibleWall.E
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Re: Psilocybe natalensis [Re: Artemisia_Ale]
    #27614289 - 01/10/22 04:36 PM (2 years, 18 days ago)

Mine are from the Grimm lineage and I always seem to get overlay problems, but I also am bacteria's bitch and wouldn't be able to say with 100% guarantee that I've actually spawned a clean jar of nats.

Anyone who has gotten prints from me, got prints from this plate pin forward.



I never got any fruits from the original print that I had other than that one. I cloned it and everything I've ran afterwards came from that culture and subsequent prints.


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