|
nosf3r4tu


Registered: 03/26/19
Posts: 775
|
|
That's contam not genetics.
|
keyesvlm
Stranger
Registered: 10/05/21
Posts: 21
Last seen: 2 years, 12 days
|
Re: Psilocybe natalensis [Re: nektar61]
#27541536 - 11/13/21 08:54 AM (2 years, 2 months ago) |
|
|
Quote:
nektar61 said:
As for liquid culture, I'd recommend getting basic teks (especially good agar tek) down before you put time and energy into that. It's easy to grow a LOT of contam quickly with LC.
Yes, I know the learning curve is steep... I expected to have lots of failures and learn from them- I did ~25 agar plates of several different varieties... out of those I had obvious contam on 2 or 3. Most the rest grew bright white myc, but slowly (perhaps my agar slightly carmelized).
From those I attempted 3 LC- One is OBVIOUSLY bacterial, one is clear liquid with white stuff RAPIDLY growing in it (from a supposed Transkei plate that grew rapidly- perhaps TOO rapidly - while the agar growth was white it looks a little different than the other slower Transkei plates- THIS is the one I worry could be mold) The 3rd LC was Natalensis, and I am pretty certain this is good- crystal clear liquid with dense white snowflakes rapidly growing (I have it on a magnetic stirrer set to low speed 24/7) while the liquid is clear, the density of the snowflakes is such you cant see through it).
My intent was to have my first grow attempt be cubensis since there is so much more documentation on what works for those- but those LC are contaminated or questionable.
So I currently have 1 good LC of Natalensis. I will re-attempt LC of the other varieties, but figure I will go ahead and get started with the Natalensis since I have prepared rye berry bags and jars. So far it seems I have the sterilization of media down, the unused plates and jars show no contam, it is my transfer process (going from print to agar, or agar to LC) that really needs improvement.
For a beginner do you feel the SFF shoebox tek ( https://www.shroomery.org/forums/showflat.php/Number/26043820 )is the way to go? Should I follow that tek with a 1:2 or 1:3 spawn:substrate ratio? Should I use CVG instead of straight coir?
|
sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
|
Re: Psilocybe natalensis [Re: nosf3r4tu]
#27541546 - 11/13/21 09:06 AM (2 years, 2 months ago) |
|
|
Quote:
nosf3r4tu said: That's contam not genetics.
I've speculated in my head that there is some wet bubble infection (mycogone perniciosa) going around the nat prints, maybe more susceptible than cubes.
|
TheDoobsker
Amateur



Registered: 05/22/20
Posts: 799
|
|
Quote:
Artemisia_Ale said: The overlay is 100% genetic too, no amount of FAE stops it.
I don't think this is true. I inoculated 6 grain jars with the same MS culture. 4 went into a 66qt mono and 2 went into a faht 16qt tub. The 16qt had 0 overlay while the mono had a small amount (for nat standards).
It seems to me that overlay is influenced by environmental factors
Edited by TheDoobsker (11/13/21 10:56 AM)
|
nosf3r4tu


Registered: 03/26/19
Posts: 775
|
|
I should of been more specific. The metabolites indicate there is contam in the sub. Imo the overlay is strictly environment. Most likely due to too much nutrition in the sub and/or improper surface conditions.
|
nosf3r4tu


Registered: 03/26/19
Posts: 775
|
Re: Psilocybe natalensis [Re: nosf3r4tu]
#27541787 - 11/13/21 12:43 PM (2 years, 2 months ago) |
|
|
I only had overlay once (the only time) when I spawned to coir at 1/2 spawn/sub and a thin top layer of 1/4" coir. The same culture that produces this on straw/manure.
|
bagga
Day Tripper


Registered: 05/19/21
Posts: 334
Last seen: 8 months, 2 days
|
Re: Psilocybe natalensis [Re: nosf3r4tu]
#27541799 - 11/13/21 12:53 PM (2 years, 2 months ago) |
|
|
Question on spore drop - these guys have dropped veils but are not producing spores. Gills are still perfectly orange.
Just need more time?
-------------------- _______________________ For personal religious use only. Embrace infinity for a healthier mind.
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 2 hours, 46 minutes
|
Re: Psilocybe natalensis [Re: bagga]
#27541804 - 11/13/21 01:00 PM (2 years, 2 months ago) |
|
|
Probably, a lot of natal likes to break the veil really early.
A lot of mine are spotty though. They’re either super heavy prints or barely there/nothing at all.
--------------------
LAGM2020     
|
bagga
Day Tripper


Registered: 05/19/21
Posts: 334
Last seen: 8 months, 2 days
|
Re: Psilocybe natalensis [Re: nosf3r4tu]
#27541822 - 11/13/21 01:07 PM (2 years, 2 months ago) |
|
|
I spawned at 1:4, 1/2 coir case at spawn. Myc blew though it.
Then another 1/4 in jiffy case at 100%. Consumed that too. Just cause I wanted to see what would happen. Ended up pinning 2in off the sub.
I also have ketchup cups that are almost entirely covered (lid included) in myc. Other cups with relatively little.
I don't think it's sub nutrition driving crazy myc. Genetics makes most sense to me based on agar behavior. From Ms some cups did it, some didn't, cups are all identical nutrition and conditions. Only thing different in those cups is genes.
-------------------- _______________________ For personal religious use only. Embrace infinity for a healthier mind.
|
smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 12 days, 8 hours
|
Re: Psilocybe natalensis [Re: bagga]
#27541826 - 11/13/21 01:10 PM (2 years, 2 months ago) |
|
|
I believe we can clone/isolate away from the overlay, as quite a few grow pictures suggest.
Harvested some big caps today
-------------------- Willpower is the one true virtue
  
|
bagga
Day Tripper


Registered: 05/19/21
Posts: 334
Last seen: 8 months, 2 days
|
Re: Psilocybe natalensis [Re: A.k.a]
#27541828 - 11/13/21 01:11 PM (2 years, 2 months ago) |
|
|
Awesome, thanks!
I'm hoping to harvest this weekend, but I also want prints. Patience...
-------------------- _______________________ For personal religious use only. Embrace infinity for a healthier mind.
|
keyesvlm
Stranger
Registered: 10/05/21
Posts: 21
Last seen: 2 years, 12 days
|
Re: Psilocybe natalensis [Re: bagga]
#27541841 - 11/13/21 01:19 PM (2 years, 2 months ago) |
|
|
Does overlay significantly impair yield?
|
bagga
Day Tripper


Registered: 05/19/21
Posts: 334
Last seen: 8 months, 2 days
|
Re: Psilocybe natalensis [Re: keyesvlm]
#27541905 - 11/13/21 02:06 PM (2 years, 2 months ago) |
|
|
I would assume so, it's a lot of wasted growth. I'm a long long way from nosferatus canopy 
Even so, the Ms yield on my 1qt tubs seems similar to ms cubes. I'm not doing monos.
If the myc is active as some have said..welllll! That would change things a lot.
Side note- the tub I put in the fc did mat and stall a little, it really doesn't like a lot of air flow that the pans were getting.
I took it out and put a lid on it, and it fruited right away. Couple days behind the tubs I left lid on.
-------------------- _______________________ For personal religious use only. Embrace infinity for a healthier mind.
|
keyesvlm
Stranger
Registered: 10/05/21
Posts: 21
Last seen: 2 years, 12 days
|
Re: Psilocybe natalensis [Re: bagga]
#27541978 - 11/13/21 03:21 PM (2 years, 2 months ago) |
|
|
Quote:
bagga said: I would assume so, it's a lot of wasted growth. I'm a long long way from nosferatus canopy 
Even so, the Ms yield on my 1qt tubs seems similar to ms cubes. I'm not doing monos.
If the myc is active as some have said..welllll! That would change things a lot.
Side note- the tub I put in the fc did mat and stall a little, it really doesn't like a lot of air flow that the pans were getting.
I took it out and put a lid on it, and it fruited right away. Couple days behind the tubs I left lid on.
That is interesting- since (and this is NOT to dispute what you say, since it obviously works for you and I have ZERO experience) I have seen on this thread claims that Natalensis likes MORE FAE... I wonder if cultivation has led to different variants where what works for one is not optimal for another...?
Do you think the SFF shoebox tek described here: https://www.shroomery.org/forums/showflat.php/Number/26043820 would work well? with a 1:3 spawn to sub ratio? I would follow that tek precisely.... but might try a few boxes with CVG substrate instead of straight coir... I am really looking for any pointers someone with experience might give me.
Thank you so much for your help- I just inoculated some grain (rye berries) with Natalensis LC... so hopefully I will be putting your advice into practice soon.
|
Guerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom
|
Re: Psilocybe natalensis [Re: keyesvlm]
#27541993 - 11/13/21 03:33 PM (2 years, 2 months ago) |
|
|
Quote:
keyesvlm said:
Quote:
bagga said: I would assume so, it's a lot of wasted growth. I'm a long long way from nosferatus canopy 
Even so, the Ms yield on my 1qt tubs seems similar to ms cubes. I'm not doing monos.
If the myc is active as some have said..welllll! That would change things a lot.
Side note- the tub I put in the fc did mat and stall a little, it really doesn't like a lot of air flow that the pans were getting.
I took it out and put a lid on it, and it fruited right away. Couple days behind the tubs I left lid on.
That is interesting- since (and this is NOT to dispute what you say, since it obviously works for you and I have ZERO experience) I have seen on this thread claims that Natalensis likes MORE FAE... I wonder if cultivation has led to different variants where what works for one is not optimal for another...?
Do you think the SFF shoebox tek described here: https://www.shroomery.org/forums/showflat.php/Number/26043820 would work well? with a 1:3 spawn to sub ratio? I would follow that tek precisely.... but might try a few boxes with CVG substrate instead of straight coir... I am really looking for any pointers someone with experience might give me.
Thank you so much for your help- I just inoculated some grain (rye berries) with Natalensis LC... so hopefully I will be putting your advice into practice soon.
I tried Pasty’s EZ dialled tubs with Natalensis and it really didn’t like it. I’m gonna be switching over to unmodified tubs which I’ve seen very good success with, so I don’t think the more FAE is particularly true.
-------------------- Being pissed on does not make you a real man.
|
fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,269
|
Re: Psilocybe natalensis [Re: Guerrilla] 1
#27542187 - 11/13/21 06:59 PM (2 years, 2 months ago) |
|
|
Printing and cloned this big boi today

|
bagga
Day Tripper


Registered: 05/19/21
Posts: 334
Last seen: 8 months, 2 days
|
Re: Psilocybe natalensis [Re: Guerrilla]
#27542194 - 11/13/21 07:07 PM (2 years, 2 months ago) |
|
|
The tubs I'm using are effectively unmod. Low fae, fast variety cubes would have massive fuzzy feet with so little but the nats seem happy.
I had intended to move them to the FC, but the myc really hated it.
Low fae might be causing smaller caps and snakey growth tho?
Here's a side by side. Lid on all week vs spent the week in with the pans.


Lid on myc is a little taller but it's fluffy, less dense, and mostly pinned on top. FC tub is just starting to pin on top (48hrs with lid on), but is mostly side pins.
Also, I'm not sure this freaky shit is wet bubble. Mutation maybe? It's a kind of regular in pattern, solid myc tissue, and a massive growth in this tub. Easily more biomass than the shrooms and growing faster.


Top and sides.
Anyway, I'm not an expert on any of this, really strange tubs, I enjoy the zaniness of it.
-------------------- _______________________ For personal religious use only. Embrace infinity for a healthier mind.
|
fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,269
|
Re: Psilocybe natalensis [Re: bagga]
#27542197 - 11/13/21 07:08 PM (2 years, 2 months ago) |
|
|
You’re growin clouds yo!
|
bagga
Day Tripper


Registered: 05/19/21
Posts: 334
Last seen: 8 months, 2 days
|
Re: Psilocybe natalensis [Re: fahtster]
#27542203 - 11/13/21 07:15 PM (2 years, 2 months ago) |
|
|
Haha yeah, here's hoping it's active🤣🥳
I think I'd rather a tub of big boyz like that fruit you snagged tbh
Will be interesting to see how nats play in a Ziploc. Are you going to try bagging them?
-------------------- _______________________ For personal religious use only. Embrace infinity for a healthier mind.
|
nektar61
Into SporePlay



Registered: 07/04/20
Posts: 3,241
Loc: Cube Satellite
Last seen: 8 days, 17 hours
|
Re: Psilocybe natalensis [Re: bagga]
#27542205 - 11/13/21 07:19 PM (2 years, 2 months ago) |
|
|
Quote:
bagga said:

Jupiter mycelium
-------------------- -NEW? Start here.
|
|