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A.k.a
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Re: Psilocybe natalensis [Re: Wall.E]
#27451971 - 09/01/21 08:55 AM (2 years, 4 months ago) |
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That’s what I was going to say.
Idk if pheno is linked to potency but mine look like those and have all been really good.
I’d clone that huge one in the middle that’s wrinkly all the way down.
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smalltalk_canceled
Babnik


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Re: Psilocybe natalensis [Re: A.k.a]
#27451978 - 09/01/21 09:01 AM (2 years, 4 months ago) |
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Yeah man that's what I thought too. Gonna give ol' wrinkles a soapy booze bath
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A.k.a
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It’s interesting how they all look the same but half are smooth and half wrinkled.
You should separate them and see if one kind seems to be stronger.
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sponglord69
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Re: Psilocybe natalensis [Re: A.k.a]
#27452338 - 09/01/21 01:44 PM (2 years, 4 months ago) |
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Bit of a tangent, has anyone experimented with natalensis extracts?
Some fellas studied natalensis (cube or species? idk)
Quote:
There were six known compounds with natural antioxidant and anti-inflammatory activities extracted from the three P. natalensis mushroom extracts.
Quote:
Compound n-hexadecoid acid with known antioxidant and anti-inflammatory activity was found to be present in all the three extracts with different degrees of area percentage.
Quote:
Ethanol extracts had the highest area % of compounds like nonadecane and tetradecane, which are known to possess anti-inflammatory and antioxidant properties along with other medicinal benefits that were not derived from the water extracts.
Here is a chart displaying the results of mass spec testing

They concluded that
Quote:
The results proposed for the first time that the water and ethanol extracts of Psilocybe natalensis are safe in the concentrations used, and have antioxidant and anti-inflammatory properties.
The study ends with this statement
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The study recommends further investigations of the mushroom effects in vivo in this field.
This is fucking interesting at the very least. It's sad that mushrooms aren't being studied further.
Obviously the study means very little in the grand scheme of things but its neat.
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smalltalk_canceled
Babnik


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Re: Psilocybe natalensis [Re: A.k.a]
#27453871 - 09/02/21 04:39 PM (2 years, 4 months ago) |
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Okay Aka, let's do this!
Together. Because Ive never succeeded in cloning.

(1)
Starting with the target. I picked one straight, one wrinkly, including a small pin attached at the stem on the wrinkly one. Put them on a dishwashed plate.

(2)
Filled a bowl with iso for cleaning the fruits.

(3)
Easy tissue first. Managed to cut loose the pin, dipped it in iso, transfered to agar.
(4)

Then to the harder tissue. These fruits were hollow sadly. No obvious super central tissue, no radiant white. Managed to get some thready tissue, dipped in iso, taken to plate.
(5)

The tissue from inside looked horrible and likely to die.
Ended up taking some fluffy tissue from the stem too.
(6)

Very hard blue/green bruising tho. Probably potent.
Hopefully I'll get some growth this time
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Edited by smalltalk_canceled (09/02/21 04:39 PM)
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A.k.a
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That’s a super gnarly mushroom.
Did you cut the mushroom open? That’s the only potential issue I can see cuz the blade will push bacteria from the skin into the stem.
Usually you can pinch the bottom of the stem and it’ll pop so you can just pull it apart perfectly in half.

My first PE clone bruised almost black while it was sitting on the new plate. It looks good though as long as you got some myc onto the agar it’ll grow.
Even if there’s bacteria the myc will outrun it pretty quick.
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Fridgedoor
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Re: Psilocybe natalensis [Re: A.k.a]
#27454547 - 09/03/21 07:55 AM (2 years, 4 months ago) |
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Harvested some bigger specimens yesterday. I wish my scale wouldn't be out of order...
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TheDoobsker
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Re: Psilocybe natalensis [Re: Fridgedoor]
#27454832 - 09/03/21 12:35 PM (2 years, 4 months ago) |
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Finally got some Nat germinated 
I took transfer from that uniform culture but thinking I should go back for the weird one next to it. Seems like some of the best Nat grows have really crazy looking myc.
Definitely gotta catch up with reading this thread so I know what to expect with this species

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smalltalk_canceled
Babnik


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So basically for hollow fruits, just forget about biopsy or a central piece of tissue?
If you just split the stem, I don't see how one is even trying to get interior fruit tissue
Edit: yeah okay I see now
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Edited by smalltalk_canceled (09/03/21 01:01 PM)
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A.k.a
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Hollow ones work the same, just not as easy as solid stems which are unfortunately rare.
Just pinch the base so it pops, then peel it in half. Then with hollow stems I like to cut right alongside the hollow area so there’s kind of a loose strip and then cut a bit of it loose.
But really anything that’s only tissue that wasn’t exposed while growing is fine.
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BrownBear
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Re: Psilocybe natalensis [Re: A.k.a] 1
#27455321 - 09/03/21 07:55 PM (2 years, 4 months ago) |
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A few P Nat tubs 3 days after spawning. I am not used to colonization being this fast. What is the typical time frame from spawn to harvest with the Nat's?
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Re: Psilocybe natalensis [Re: BrownBear]
#27455343 - 09/03/21 08:16 PM (2 years, 4 months ago) |
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Depends what kind of overlay you get, but they’re usually pretty fast. At least a few days quicker than cubes.
Unless you get the horrible overlay and then it could be over a month.
Right now it’s the limbo phase where you wait to see if those rhizo fingers are going to swell up into a mat or if it’ll just knot up and pin.
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BrownBear
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Re: Psilocybe natalensis [Re: A.k.a]
#27455352 - 09/03/21 08:25 PM (2 years, 4 months ago) |
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Thanks, AKA. I appreciate the info.
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smalltalk_canceled
Babnik


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Re: Psilocybe natalensis [Re: A.k.a]
#27455903 - 09/04/21 11:59 AM (2 years, 4 months ago) |
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Wow so easy working with Aka , natalensis clone culture in 1 try
4/9 showing radiant mycelial growth

Much easy, cleanest of growths, no problemo isopropanolo.
Pin unsurprisingly showing best amount of growth and appearance:
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Edited by smalltalk_canceled (09/04/21 12:11 PM)
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smalltalk_canceled
Babnik


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Things moving forward real fast with natalensis, can you dig this growth *just * since the last post?
Its the natalensis "double growing" not me double posting.

It's time to expand the clone. Not much to say here avoided the bottom area, visible halo, the other had great looking ropey nice looking mycelium
Although I don't understand why doing it like this makes it a clone. The growth I'll be taking looks like any good mycelium.
Why does using mycelium from tissue make it a clone? Love to be able to explain this to my family or friends.
Why is taking mycelium grown from fruits different in the amount of genes or difference the transfer can express, from mycelium pre fruiting?
Edit: finally managed to narrow my question down
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Edited by smalltalk_canceled (09/05/21 07:14 PM)
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jomanda1990
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The mycelium that makes up fruiting bodies contains much less genetic variability than the mycelium that grows from spores. As you transfer again and again from a germination plate (spores), you are progressively reducing the genetic variability in the mycelium. A germination plate can contain millions of individual strains, but after, say, five transfers, you might have narrowed this down to 100 (numbers pulled directly from my arse just for illustrative purposes) I've read several times that fruiting bodies not always contain exclusively one genome and are actually a number of individual genomes cooperating, but I haven't seen any studies explaining it in detail.
A similar way to think about it is when you 'clone' succulent plants from individual leaves. The leaf produces an entire plant that has the exact same genome of the parent plant. The same essentially happens with the fruiting bodies of mushrooms, except that, as I said above, they may be 'multiple individuals' in a single fruiting body.
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Edited by jomanda1990 (09/05/21 07:17 PM)
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Wall.E
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Quote:
jomanda1990 said: The mycelium that makes up fruiting bodies contains much less genetic variability than the mycelium that grows from spores. As you transfer again and again from a germination plate (spores), you are progressively reducing the genetic variability in the mycelium. A germination plate can contain millions of individual strains, but after, say, five transfers, you might have narrowed this down to 100 (numbers pulled directly from my arse just for illustrative purposes) I've read several times that fruiting bodies not always contain exclusively one genome and are actually a number of individual genomes cooperating, but I haven't seen any studies explaining it in detail.
A similar way to think about it is when you 'clone' succulent plants from individual leaves. The leaf produces an entire plant that has the exact same genome of the parent plant. The same essentially happens with the fruiting bodies of mushrooms, except that, as I said above, they may be 'multiple individuals' in a single fruiting body.
There are hundreds of different dikaryotic strains inside a single mushroom, so it’s not you lol. This is why sometimes a clone will be exactly what you want or sometimes it can be just as various as a multispore grow. I’ve take clones from nats that will fan out in rhizomorphs on one side and be totally tomentose on the other side of the plate.
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jomanda1990
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Re: Psilocybe natalensis [Re: Wall.E]
#27457712 - 09/05/21 10:00 PM (2 years, 4 months ago) |
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Wow, hundreds of strains in a single fruit? I was expecting something below a dozen lmao.

So as you keep transfering to clean and/or expand clone cultures, you keep narrowing down the strains bit by bit? Or are they so intertwined/entangled that the little piece of colonized agar you take in your transfer has "all the necessary strains" that the source fruit body had? This is really interesting tbh
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Wall.E
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Nah you had it right, it’s just slimming down genetics and isolating. As far as I know (I’ve never slanted a proven clone so I’m not one to speak from experience) you have to clone a few times to get a solid performer. I think some people get lucky sometimes and have to do it once or twice but I’m not sure
And you could be right about it being closer to a dozen than my hundreds guess, I’ve posed this question fifty times in fifty different ways to get no one to answer me. So I’m just throwing that number out there, but since shit’s happening on a cellular level, it would be hard to really pinpoint the numbers at home.
I wrote this and then I tried cloning to prove my point but could never get nats to perform reliably lol
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A.k.a
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Re: Psilocybe natalensis [Re: Wall.E]
#27457950 - 09/06/21 07:01 AM (2 years, 4 months ago) |
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Some people think that the mycelium mutates often enough that even if you did manage to isolate down to one strain that it would mutate and start sectoring again.
It’s kind of shocking how little is known about mushrooms.
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